From the roots of phytolacca esculenta, six triterpenoid saponins were isolated. On the basis of spectral analysis and chemical properties, four of them were identified as 3-O-(?-D-glucopyranosyl-?-D-xylopyranosyl)-jaligonic acid-30-methyl ester, 3-O-?-D-xylo pyranosyl-jaligonic acid-30-methyl ester, 3-O- (?-D-glucopyranosyl-?-D-xylopyranosyl) -esculentic acid-30-methyl ester and 3-O-?-D-glucopyranosyl-jaligonic acid -30-methyl ester,and in sequence tentatively named as esculentoside"Jia""yi""bing'' and"ding" respectively.Pharmacological experiments showed that the total saponins and esculentoside "Jia" exert a considerable enhancement on phagocytic function of leucocytes and that the total saponins can promote DNA synthesis to make the DNA which was decreased by hydroxy-urea keep a normal level.

Six compounds were isolated from the whole herb of Phyllanthus urinaria L. They were id entified as ellagic acid (Ⅰ), 3, 3', 4-tri?O-methylellagic acid (Ⅱ), succinic acid (Ⅲ ), feru lic (Ⅳ ), ?-sitosterol-glucoside (Ⅴ ) and gallic acid (Ⅵ ) by means of spectral analysis and chemical reaction- Compound Ⅱ, Ⅲ, Ⅳ, and Ⅴwere isolated for the first time from this plant

Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .

AIM　To research the chemical constituents from dried roots of Uncaria yunanensis Hsia.C.C. METHODS　Modern chromatography was used to isolate chemical components. Their structure were identified by spectral analysis. RESULTS　Seven compounds were isolated and identified as 3β,6β,19α-trihydroxyurs-12-en-28 oic acid (I), 23-nor-24-esomethylene-3β,6β-19α-trihydroxyurs-12-en-28 oic acid (II), 3-oxo-6β,19α-dihydroxyurs-12-en-28 oic acid (III), oleanic acid (IV), 5,7,3′,4′-tetrahydroxy-flavan-3-ol (V), β-yohimbine (VI) and diangoutengjian I (VII). CONCLUSION　All of the above compounds were isolated for the first time from the root of this plant. Among them, compound VII is a new one.

Fresh hotbed chives solutions were extracted respectively by ether, acetic ether and n-butyl alcohol and protein constituents and non-protein constituents were obtained. Ether extractions (non-protein constituents) had the strongest antirriutagenic effects by the SOS chromotest Non-protein constituents of hotbed chives were analysed further by gas chromatography-mass spectrum and 30 chemical substances were isolated and identified. Their antimutagenic substances may be dimethyl disulfide, 4-methyl-2-pyridinethione, di-2-propenyl trisulfide, 3-(allylthio)-propionic acid.

Many constituents with novel structures have been isolated from marine brown algae,some of which possess various bioactivities.The terpenoids,lipids,steroids,phlorotannins and some other compounds isolated from brown algae through 1993~2000 were reviewed in this article.

Three active compounds were isolated from the marine bryozoan Bugula neritina living in the South China Sea by bioassay-guiding isolation method with a combination of extraction with suitable solvent and multiple column chromatography(Sephadex LH-20, ODS and preparative HPLC). Their structures were assigned as known bryostatin 4, bryostatin 5 and bryostatin 6 by intensive analysis of the data of high resolution 2DNMR(600 MHz,DQF-COSY,TOCSY,HMQC and ROESY). All these compounds were obtained from this bryozoan in the South China Sea for the first time and showed significant antineoplastic activities in vitro.

Many marine steroids with novel structures have been isolated, some of which possess various bioactivities. The new compounds reported through 1996~2000 were reviewed in this article.

Objective: To investigate the influence of esculentoside A（EsA） on production of IL-1 and TNF by rabbit synovial cells induced by LPS. Methods: levels of IL-1 and TNF in the supernatant of rabbit synovial cell were determined by examining proliferation of thymic cells and by bioassay L929 cells as target cells, respectively. Results: EsA in 5-40 μg/ml could significantly inhibit the production of IL-1 and TNF from rabbit synovial cells induced by LPS. Conclusion: EsA can inhibit the production of IL-1 and TNF from synovial cells. It suggests that EsA may play a role in improving the rheumatoid arthritis.

Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity.Methods Triterpene glycosides from H.scabra were separated and purified by chromatography on DA-101,silica gel,and reversed-phase silica gel column,as well as RP-HPLC.Their structures were elucidated on the basis of spectral data and chemical evidence.Results Three triterpene glycosides were identified as scabraside D (1),fuscocineroside C (2),and 24-dehydroechinoside A (3).Their inhibition on P-388,A549,MKN-28,HCT116,and MCF-7 cells were significant.Conclusion Scabraside D (1) is a new triterpene glycoside,and compounds 2 and 3 are isolated from H.scabra for the first time.The glycosides 1-3 show the in vitro cytotoxicity against five human tumor cell lines in comparison to 10-hydroxycamptothecin.