Many marine steroids with novel structures have been isolated, some of which possess various bioactivities. The new compounds reported through 1996~2000 were reviewed in this article.

Many constituents with novel structures have been isolated from marine brown algae,some of which possess various bioactivities.The terpenoids,lipids,steroids,phlorotannins and some other compounds isolated from brown algae through 1993~2000 were reviewed in this article.

In this paper, a review and prospect to the progress in the studies on the chemical structures, pharmacological activities, methods of isolation and structural elucidation of as-terosaponins from starfish were reported.

Objective To investigate the chemical constituents of the sea cucumber Holothuria impatiens collected from the South China Sea.Methods The pure compounds were isolated by extraction,partition and multi-chromatography.Their structures were elucidated on the basis of spectral analysis and chemical evidence.Results Three triterpene glycosides were isolated and identified as holothurin A1(Ⅰ),holothurin A(Ⅱ) and pervicoside C(Ⅲ),together with a physiological substance of adenosine(Ⅳ).Conclusion All were isolated from Holothuria impatiens for the first time.Three glycosides showed significant cytotoxic activities.

Micromachining of capillary electrophoresis chips is described.The current in channels was determined versus the voltage applied on the chip.Using laser-induced fluorescence detection,the process of injection and separation on chips was recorded with charge-coupled device(CCD).With defferent voltge of reservoirs on glass chips,profiles of the sample plug were studied. The elctropherogram of hydrolysis product of fluorescein isothiocyanate (FTTC-OH) and arginine labeled by fluorescein isothiocyante(FTTC-Arg) was obtained.

Objective To investigate the surgical methods and clinical effect of repairing forefoot soft tissue defects with free peroneal artery perforator flap in elderly patients.Methods From June,2011 to April,2015,17 cases of forefoot soft tissue defects repaired with free peroneal artery perforator flap in elderly patients.There were 10 cases of male and female in 7 cases with an average age of 65.8 years old ranging from 60 to 74 years.Causes of injury:traffic accident in 7 cases,heavy crushing in 9 cases,electrical bums in 1 case.Injury part:6 cases on the left side and 11 cases on the right side.Metatarsus and phalanges fracture in 9 cases,tendon injury in 5 cases.Defect area:3.0 cm × 4.0 cm-6.3 cm × 11.2 cm.Results All flaps survived.All wounds were primary healing.Skin graft survived for the foot flap donor site,and no complicated with infection.All patients were followed up from 8 to 36 months with an average of 17.6 months.The appearance of flaps were good,slight bloated.The texture and color of the flaps were close to the recipient site.Flap feel were good.Accortling to (AOFAS)criteria system,the AOFAS score of last follow-up was (77.5±13.2).Excellent in 6 cases,good in 9 cases,fair in 2 cases.VAS score was (2.6±0.4).Conclusion The free peroneal artery perforator flap with the advantages of vascular anatomy constant,blood supply is reliable,thickness moderate,etc.It is a useful clinical method to repair forefoot soft tissue defects in elderly patients.

Objective:To screen for the bioactive marine organisms and to study the active constituents from them.Methods:Bioactivities of marine organisms extracts were screened by Pyricularia oryzae bioassay method and the constituents were isolated and identified by a combination of multi chromatography and spectral analysis.Results:Twenty ethanolic extracts and 5 aqueous extracts of the tested marine organisms showed activities causing morphological abnormality of P.oryzae mycelia.Fifty six compounds were obtained and identified from Sargassum carpophyllum,Ishige okamurai and Natarchus leachii freeri ,among which 28 compounds showed activities against P.oryzae and exhibited cytotoxicities on various cultured tumor cell lines,eight compounds showed inhibition effects on Fonsecaea pedrosoi in vitro .Conclusion:The screening data and bioactivity evaluation suggest the P.oryzae bioassay is suitable for preliminary screening of bioactive agents from marine organisms.

Objective:To explore the effect of self-efficacy enhancement in patients with femoral neck fracture.Methods:Using the convenience sampling, 80 patients with femoral neck fractures admitted to Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine from January 2019 to March 2021 were selected as the research object. According to the admission time, 40 patients admitted to the hospital from January to December 2019 were selected as the control group, and 40 patients admitted to the hospital from January 2020 to March 2021 were selected as the observation group. The control group received routine nursing, while the observation group was given self-efficacy enhancement. The differences in Self-efficacy Rehabilitation Outcome Scale (SER) scores, functional exercise compliance and Harris Hip Score (HHS) scores at different time points were compared between the two groups.Results:The SER score of the observation group was higher than that of the control group at the time of discharge, one month after operation, and three months after operation ( P<0.05) . The functional exercise compliance scores of the observation group were higher than those of the control group at the time of discharge, one month after operation, and three months after operation ( P<0.05) . The HHS score of the observation group was higher than that of the control group at the time of discharge, one month after operation, and three months after operation ( P<0.05) . Conclusions:Self-efficacy enhancement can enhance the self-efficacy of patients with femoral neck fracture, improve their compliance with functional exercise, and promote functional recovery of the hip joint.

Objective To investigate the clinical efficacy of Zhuangjin Yangxue decoction on Hepple stage Ⅰ and Ⅱ of osteochondral lesion of the talus(OLT).Methods From February 2021 to December 2022,a total of 62 cases of OLT were seleted from the Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine.According to random number table method,the patients were divided into observation group and control group,with 31 cases in each group.The control group was treated with Western medicine,and the observation group received an additional treatment of Zhuangjin Yangxue decoction alongside with Western medicine.Serum matrix metalloproteinase 13(MMP-13),tumor necrosis factor-α(TNF-α),ankle visual analogue scale(VAS)score,American Orthopedic Foot and Ankle Society(AOFAS)ankle-hind foot function score,TCM syndrome score and magnetic resonance observation of cartilage repair tissue(MOCART)score before and after treatment were compared between two groups.Results After 5 weeks and 3 months of treatment,MMP-13,TNF-α,VAS score and TCM syndrome score in two groups were significantly lower than before treatment,and AOFAS ankle-hind foot function score and MOCART score were significantly higher than before treatment(P<0.05).MMP-13,TNF-α and TCM syndrome scores in observation group were significantly lower than those in control group,and MOCART score was significantly higher than that in control group(P<0.05).After 5 weeks of treatment,VAS score of observation group was significantly lower than that of control group,and AOFAS ankle-hind foot function score was significantly higher than that of control group(P<0.05).Conclusion Zhuangjin Yangxue decoction shows a good short-term efficacy in treating Hepple stage Ⅰ and Ⅱ of OLT,with anti-inflammatory and analgesic effects,improved joint function,and promotion of cartilage repair.

Objective To observe the clinical efficacy of muscle energy technology in the rehabilitation of ankle fracture surgery.Methods From March 2021 to October 2023,98 patients with ankle fracture who underwent surgical treatment in Hangzhou Xiaoshan District Hospital of Traditional Chinese Medicine were selected.According to the difference of rehabilitation program after surgery,they were divided into observation group and control group,with 49 cases in each group.The control group was treated with routine rehabilitation training,and observation group was treated with muscle energy technology.The clinical efficacy,American Orthopaedic Foot and Ankle Society(AOFAS)score,ankle pain visual analogue scale(VAS)score,ankle active range of motion,and bone metabolism indexes[osteocalcin(OCN),bone-specific alkaline phosphatase(BALP),C-terminal telopeptide of type β-Ⅰ collagen(β-CTX)]were compared between two groups.Results Compared with control group,AOFAS score significantly increased and VAS score significantly decreased in observation group(P＜0.01).The active plantar flexion and dorsal extension range of motion of ankle joint in observation group were better than those in control group(P＜0.01).Compared with control group,the values of OCN and BALP in observation group increased significantly(P＜0.01),the value of β-CTX decreased significantly(P＜0.01),and the excellent rate of AOFAS score was higher than that in control group(P＜0.05).Conclusion Muscle energy technology can effectively relieve joint pain after ankle fracture surgery,regulate bone metabolism indicators,and improve ankle function.