Objective To establish a HPLC method for the determination of effective components in Radix Notoginseng in San Xiong Zhitong Ointment.Methods Kromasil KR100-5 C18 column(250 mm?4.6 mm,5 ?m)and gradient elution were used.Solvent A was acetonitrile and solvent B was water.The detection wavelength was set at 203 nm.Results The four components were isolated well.The linearity was fine with the recoveries of 98 %～100 %.Conclusion The quantitative method for determining the ingredient of San Xiong Zhitong Ointment is simple,feasible and repeatable,and is beneficial for quality control of San Xiong Zhitong Ointment.