The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480 cells were used as the research object, and untreated SW480 cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos-phate determination methods were used to detect proliferation, invasion and Na+-K+-ATPase activity expression level in SW480 cells. Results The physiological concentration of cisplatin (70 μmol/L) inhibited the proliferation of SW480 cells at 48 h. There was no significant difference in the inhibition rate at 48 h compared with 72 h and 96 h. Treatment with 70 μmol/L cisplatin for 48 h reduced the number of cells through Matrigel membrane matrix. The Na+-K+-ATPase activity was significantly increased in 35, 70 and 140 μmol/L cells after treatment with 17.5, 35, 70 and 140 μmol/L of cisplatin in SW480 cells for 48 h, and Na+-K+-ATPase reached the highest level at 70 μmol/L of cisplatin. Conclusion The decreased activity of Na+-K+-ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.

Protein fingerprinting technology(PFT) is a novel technology for laboratory diagnosis developed in recent five years.It has advantages of simple operation,testing quickly,high sensitivity and specificity.It is a revolutional progress for laboratory diagnosis.The application of PFT in medical field is mainly for the detection of diseases.The sensitivity and specificity in cancer detection are about 80%.Immunomic mass spectrometry(IMS) is a novel technology using the combined group antibodies for capture multi biomarkers and applying mass spectrometry to precisely analyze the modification or isoforms of the biomarker in single platform,whereas traditional assay could not be able to identify the variation of biomarkers.PFT and IMS have significantly influenced in cancer early detection,especially to evaluate cancers which did not express traditional tumor markers like AFP,CEA,etc.PFT and IMS have characteristics of early detection in the molecular and gene level.PFT and IMS are diagnostic technologies with bright future and potential applications.

The proteins and antigens of nine strains of Toxoplasma gondii were analyzed by SDS-PAGE and immunoblotting. These strains including RH strain, Zs-2 strain, CN strain and SHI, SH2, SH3, SH4, SH5, SH8 strains, were obtained from other laboratories or isolated from deformity fetus in our laboratory. SDS-PAGE analysis revealed that these strains were very close in the major bands of proteins and differences were observed only in part. There were common components in antigens of all strains using IgG antibody prepared from high-titre rabbit antisera raised against RH strain of Toxoplasma.

Objective The quantitation measurement of microalbuminuria was adopted to verify the feasibility of urinary microalbumin and ratio of mieroalbumin to creatinine (M/C) detection with dry hemieal method. Methods Totally 80 urine samples from the patients with clinically diagnosed dia-betes were tested by the immune quantitation method (immunoturhidimetric assay) and dry heroical method (HT2000 urine analyzer and test paper, Guilin Hnatong Company) simultaneously. Sarcosine oxidase method was applied to measuring creatinine level. Ten cases of outliers were removed. With the quantitation result of M/C as the reference standard, immune quatilyzation of the M/C as refer-ence, we compared and analyzed the results of the immune quantilization of urine microalbumin, those of the dry chemistry method and of the dry chemistry system detecting M/C. Results There was sig-nificant differences in test results of urinary microalbumin with the dry chemistry method and the im-mune quantitation method (P<0.01) The sensitivity, accuracy and Youden index of the microalbu-minuria testing had been observed to decrease in order in immune the dry chemistry method, the im-mune quantitation method and M/C quantitation detection method. Conclusion The sensitivity of semi-quantitation dry chemical method is satisfactory in detecting microalbumin, which may be used as a means of microalbumin screening. M/C detection with the dry chemical method isn't suitable for screening microalbumin in instant urine samples.

Intracerebral hemorrhage (ICH) is a common nervous system disease, its mortality and disability are very high. However, the mechanisms of brain injury after ICH have not yet been fully explained. "[he latest studies have suggested that iron overloading plays an important role in the brain injury after ICH. This article reviews the advances in research on the distribution and function of iron in the brain, the mechanisms of brain injury caused by iron overloading after ICH as well as the use of iron chelator.

ObjectiveTo investigate the effect of thalidomide on the proliferation and apoptosis in human pancreatic cancer cell SW1990 in vitro.MethodsSW1990 cell line was treated with thalidomide at different concentrations (3.125,6.25,12.5,25,50,100,200 and 400 μg/ml) for 24,48,72 h,and then cell proliferation were evaluated by MTT.Cell cycle was determined by flow cytometry,apoptosis was determined by annexin V/PI fluos staining,Bcl-2,Bax protein expression and Bcl-2/Bax ratios were measured by Western Blot in vitro.ResultsThalidomide inhibited the proliferation of SW1990 cells in a time and dosedependant manner.The proportion of G0/G1 phase of SW1990 cells with 200 μg/ml thalidomide treatment increased from (41.15 ± 2.23 ) ％ to (58.83 ± 2.33 ) ％,apoptosis rate increased from 2.6％ to 28.0％,the expression of Bax protein up-regulated from 0.17 ± 0.03 to 0.33 ± 0.04,the expression of Bcl-2 protein downregulated from 0.35 ± 0.02 to 0.17± 0.01,the ration of Bcl-2/Bax decreased from 2.17 ± 0.44 to 0.52 ±0.07.ConclusionsThalidomide can inhibit the proliferation of pancreatic cancer SW1990 cells by upregulating Bax,down-regulating Bcl-2,inducing cell apoptosis and cell Go/G1 phase arrest.

ObjectiveTo discuss the incidence of negative emotion and behavioral problems between left-behind and non-left-behind children in rural areas during hospitalization. Methods175 participants included 90 cases of non-left-behind children patients and 85 left-behind children patients.The negative emotion and behavioral problems of all children were recorded and compared. ResultsCompared with the non-left-behind children patients,left-behind children manifested negative emotions such as anger,anxiety,depression and fear,more probability and incidence of behavioral problems such as dysphonia,special learning disabilities,sleep disorders,twitch disease,multiple tourtte's syndrome,attention deficit disorder,enuresis sickness and so on.There was significant differences between the two groups. ConclusionsCompared with the non-left-behind children,left-behind children patients are more likely to have negative emotion and behavioral prob-lems,it may have lasting influence on children and eventually influence the development of the physical and mental health,cause learning and adaptation difficulties.So more care and attention should be given to left-behind children,and given timely discovery and timely treatment of negative emotions and behavioral problems.

Purpose　To explore the value of single cell isolation from tissue slides and single cell polymerase chain reaction(PCR) in the study of lymphy node germinal centers.　Methods　By single-cell isolation and single-cell PCR,rearranged immunoglobulin(Ig) genes were amplified from single Ki67(+) cells and single CD3(+) cells.The Ⅴ-region family specific primers were designed to Ig heavy chain leader region and light chain κ and λ framework region Ⅰ.　Results　PCR efficiency of Ki67(+) cells were 18.7% and 50.0% respectively in 2 lymph nodes.However,in 40 CD3(+) T cells,no Ig gene rearrangements were observed,which confirmed the efficiency and specificity of single cell isolation and single cell PCR.　Conclusions　Our study demonstrated the feasibility of single cell isolation from tissue slides and single cell PCR.This makes a sound basis for the use of technique in the further study of lymphoid diseases.

Animals ; Dental Implantation, Endosseous ; Dental Implants ; Dental Implants, Single-Tooth ; Denture, Complete, Immediate ; Denture, Partial, Immediate ; Humans ; Immediate Dental Implant Loading ; methods ; Jaw, Edentulous ; rehabilitation ; Jaw, Edentulous, Partially ; rehabilitation ; Mandible ; Maxilla