Objective To explore the clinical application of anticoagulation therapy for patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods 55 patients with AECOPD were selected and divided into control group(24 cases) and treatment group(31 cases).Oxygen treatment,anti-infection treatment were applied to the patients of the control group.Treatment group was given anticoagulation treatment with heparin at the base treatment of the control group.The treatment efficacy,pulmonary functions,coagulation functions and inflammatory factors were tested and compared.Results There were no significant differences between two groups of total treatment efficacy(80.65％ vs 79.17％,x2 =1.304,P ＞0.05) ;PaCO2 decreased,pH and PaO2 increased significantly after treatments in the two groups(all P ＜ 0.05),which was more significantly in the treatment group (all P ＜0.05) ;FVC,FEV1 and FEV1/ FVC％ increased significantly after treatments in the two groups(all P ＜ 0.05),more significantly in the treatment group (all P ＜ 0.05) ; APTT,PT were prolonged,the level of DD and PAD decreased significantly after treatments in the two groups(all P ＜ 0.05),which was more significantly in the treatment group(all P ＜ 0.05) ;The level of IL-6,IL-10 and hs-CRP decreased significantly in all patients(all P ＜ 0.05),IL-6and hs-CRP were more significantly in the treatment group (all P ＜ 0.05),but the level of IL-10 of the treatment group was higher than that of the control group (P ＜ 0.05).Conclusion Pulmonary functions,Blood-gas indexes could be improved,inflammatory balance and coagulation status could be maintained by anti-coagulation treatment in patients with AECOPD.

Objective:To prepare levofloxacin-carboxymethyl-chitosan(LVFX/CMC) microspheres and to study their colon-targeted release in rats.Methods: KromasilRKR100-5C18(250 mm?4.6 mm)was used as the analytical column and the temperature was maintained at 50℃.The mobile phase consisted of a mixture of acetonitrile:0.1% trifluoroacetic acid(2080) pumped at a flow rate of 1.0 ml/min,with ?_(ex)295 nm and ?_(em)495 nm.Sixty healthy male SD rats were randomized into 2 groups: to receive gastric lavage with normal LVFX(40 mg,control group) and LVFX/CMC microspheres(40 mg,treatment group).HPLC was used to detect the concentrations of LVFX in the stomach,intestine,cecum,colon and blood in rats in both groups.Results: LVFX was detectable 3 h after LVFX/CMC microsphere lavage.Five hours after lavage,the levels of LVFX in cecum and colon were respectively(3.394?0.197) mg and(1.873?0.216) mg in treatment group,and(0.489?0.123) mg and((0.078?0.002)) mg in control group(P

Objective:To culture mesenchymal progenitor cells(MPC)from compact bone fragments in C57 mice in vitro and to study the feasibility of inducing directed differentiation of MPC into neuron-like cells in vitro. Methods:Bones of hind limbs of C57 mice were sheared into bone fragments and digested by collagenase type Ⅱ. Then,MPC were cultured in vitro and analyzed by flow cytometry for identification of its immunology phenotype. MPC of P3 in good growth were induced directionally by the supernatant cultured with primary neuron,and then detected the expression of neuronal specific markers neuron specific enolase(NSE)and neurofilament protein(NF)by immunocytochemical staining. Results:The primary MPC were cultured successfully and they grewwell after passage. Compared with that of the control group,the positive rate of CD29 and CD44 of MPC of antibody group had significant difference (P

Sanger sequencing technology is the most commonly used method for genetic analysis in inherited eye disease in the past few decades on account of its long DNA sequencing read length and high accuracy.However,application of this method is limited in genetically heterogeneous diseases because of the high economic and time cost.Next generation sequencing (NGS) technology is a high-throughput,cost-effective,and highly automated method which has been widely used since 2005.Although NGS is especially suitable for studies on genetically heterogeneous inherited eye disease,overdependence on the technique itself and neglect of the assessment of the phenotype may lead to misjudgment of the testing results and higher economic burden for the patients.So far,the most common method on researches of monogenic inherited eye disease is to combine NGS and Sanger sequencing technique.Precise evaluation of the clinical phenotype of patients is very important,as it is related with selection of detection methods and determination of disease-causing mutations.

Objective To study the possibility and effect of application of clinical pathways (CP) in patients with lumbar vertebrae protrusion undergoing resection of nucleus pulposus. Methods 280 patients with lumbar vertebrae protrusion undergoing resection of nucleus pulposus were selected and divided into the experimental group and the control group randomly. Each group had 140 cases. Clinical pathways was applied to the experimental group, while traditional nursing method was given to the control group. Treatment and nursing effect were compared between the two groups. Results The cost for hospitalization was lower, hospitalization time was shorter, the first time for functional exercise after operation was advanced and satisfaction degree of patients was increased in the experimental group compared with those of the control group. Conclusions Application of clinical pathway in patients with lumbar vertebrae protrusion undergoing resection of nucleus pulposus played a positive and effective role.

Objective To study the prevention of pressure ulcer for bed-ridden patients by simple turn-over pillow and turn-over mattress.Methods Self-made simple turn-over pillow and turnover mattress were used in 1300 patients who had high risk of pressure ulcer,and the application effect was observed.Results No pressure ulcer occurred in 1300 patients when they were discharged.Conclusions Simple turn-over pillow and turn-over mattress showed definite effect to prevent pressure ulcer,they have application value,and are worthy of wide spread.

The papers on information ecology theory in foreign and domestic libraries were analyzed and its appli-cation value in book acquisition was studied.The information ecosphere formed by readers, acquisition members and experts was put forward and how to make the information ecosystem reach its new balance by dynamic regula-tion of its main components through information flow.

To introduce the academic thoughts of traditional Chinese medicine(TCM)master GUO Zi-guang based on the analysis of clinical cases.His main academic thoughts are:①He proposes the theory of six meridians and their differentiation in the diagnosis and treatment of typhoid.The six meridians are bases for the treatment of all diseases,and their differentiation provides a fundamental principle for the differentiation of diseases.②He uses the method of combining the disease and syndrome in diagnosis and treatment,which widens the horizon of traditional differentiation and diagnosis in TCM and avoids the absence of syndrome in the practice of differentiation.In this way,the therapeutic effects of Chinese medicine can be guaranteed so as to meet the needs of medical and social development.③He emphasizes that it is fundamental to improve the therapeutic effects of TCM for its development.The approach is to combine disease and syndrome in diagnosis and treatment,and the way is to apply different methods in disease treatment.

Pelvic congestion syndrome plagues women's physical and mental health,the morbidity rate has increased in recent years.Based on TCM differentiation and classifications of syndrome,pathogenesis of pelvic congestion syndrome is divided into‘dampness-blood stasis’and‘deficiency of qi resulting blood stasis’2 types.Our Qushi Huayu Decoction and Yiqi Tongyu Decoction have been clinically proven to relieve pain,improve quality of life of the patients,and achieve better clinical effect.

Paeoniflorin in Chinese traditional patent medicine "Moluo granules" was determined byreversed phase HPLC.Optimum separation was achieved by employing C18 column as the stationary phase,MeOH-(CH3)2 CHOH-36% HOAc-H2O(25∶2∶2∶71) as the mobile phase.Theaverage recovery is 99.06%,and the coefficient of variation is 1.04%,The extraction is simple,the analytical method is rapid and accurate with good reproducibility.