Humans ; Occupational Health ; Protective Devices

Objective Rat glial cell derived neurotrophic factor(GDNF) gene and tyrosine hydroxylase(TH) gene were widely used in gene therapy of Parkinson's Disease(PD).The present study was designed to investigate whether the two target genes GDNF and TH could express simultaneously in one adeno-associated virus(AAV)vector and to discuss the probability of using it in gene therapy of PD.Methods RNA of GDNF and TH in HEK293 packaging cells were examined by reverse transcription-polymerase chain reaction(RT-PCR).Expressions of GDNF and TH in infected bone marrow stromal cells(BMSCs) and rat brain coronal sections were examined with immunocytochemistry and immunohistochemistry assay.Results RNA of GDNF and TH were detected in HEK293 packaging cells.Expressions of this two genes were observed in both bone marrow stromal cells and brain sections of rats.Theexpression efficiency of AAV-LacZ was about 50% in HEK293 packaging cells and approximately 15% in infected BMSCs and the expressions of TH and GDNF on the AAV-GDNF/TH virus injected points both had significant differences(P

Objective To investigate the ECG atypical changes in acute myocardial infarction(AMI). Meth-ods 352 patients with AMI were selected sequentially and examined by electrocardiogram(ECG) ,Holter of 24h,my-ocardial enzymes testing and coronary angiography, and electrocardio-monitoring was performed at the same time. Data were analyzed by statistics. Results The incidence of ECG atypical changes in patients with AMI was 14. 5%. The ECG atypical changes including Q wavelet with the elevation value of ST segment, the delays of Q wave and the only changes of ST-T. Conclusion The above several kind of ECG performane are present in atypical AMI.

This paper discusses the meaning of the tutorial system on clinical practice;the qualifications,tasks and a responsibility of the tutors,expounds the role of tutors,and discusses the main problems of tutorial system of the undergraduate.

Objective To study the inductive effect of targeted UVB phototherapy on skin hyperpigmentation and its mechanism.Methods Ten brownish guinea pigs were used to develop experimental models.After depilation,four adjacent areas were selected on the back of each guinea pigs and served as the control,low-dose,moderate-dose and high-dose group to receive targeted UVB irradiation with a cumulative dose of O,2500,3500,4500 mJ/cm2,respectively.After 6-week irradiation,the guinea pigs were sacrificed and skin sampies were obtained.The hyperpigmentation induced by UVB irradiation was estimated by naked eyes,staining for melanocytes(Imokawa method)and melanin granules(Masson-Fontana staining),respectively.Immunohistochemistry was carried out to determine the level of nitric oxide synthase and HE staining to observe epidermal histological changes.Results A statistical difference was observed in the pigmentation score,quantity of melanin granules and dopa-positive melanocyte number among the four groups (P<0.05),and the moderatedose group was higher than the high-dose group in terms of these parameters.The expression of inducible nitric oxide synthase increased in a radiation dose-dependent manner,and the median value for inducible nitric oxide synthase expression level was 0.50,1.25,1.75,2.00 in the control,low-dose,moderate-dose and highdose group,respectively(P<0.05).Conclusions Targeted UVB phototherapy can induce hyperpigmentation of the skin in brownish guinea pigs in a dose-dependent manner,but higher dose may not work better.To irradiate with an initial dose close to or slightly higher than the minimum erythema dose may result in a satisfactory effect with reduced cumulative dose and potential risk for cancer.

Objective To study the influence of colon hydrotherapy on premature infants with jaundice.Methods Based on intravenously infusing yinzhihuang and albumin and enzyme inducer,the warmed normal saline was poured into colon by 10 mL/kg per 8 hours in 40 preterm infants,and compared with 44 cases of control group.Serum bilirubin of all patients were measured at 24,72,120,168,216 hours,respectively.The days of meconiumbeing eliminated completely were recorded.Results The bilirubinemia level in trial group were lower in trial group than that in control group at every time and the rate of hyperbilirubinemia of the test group was lower(?~2=4.073 P

With increasing attention to pains, the measurement of pain threshold has become an important part among researches to pains. Therefore, measuring Methods and their validity as well as reliability have direct influenced the Conclusion of the researchs. The approaches of pain threshold measurements, and the factors influenced the result were discussed in this paper.

ObjectiveTo observe the effect of early rehabilitation and insulin treatment on acute cerebral infarction. Methods160 cases with acute cerebral infarction (without diabetes and the serum glucose were all above 6.7 mmol/L) were divided into treatment group and control group. Each case of both groups was treated with routine treatment, and those in the treatment group were treated with insulin instantly and early rehabilitation in the first day after onset when physical signs were stable and conditions didn't deteriorate. They were assessed with Neurological Impairment, Fugl-Meyer Assessment and Barthel Index 4 weeks after treatment. ResultsCompared with the control group, the neurological function improved obviously in the treated group (P＜0.05). ConclusionEarly rehabilitation and insulin treatment can improve neurological function and the activity of daily living.

Objective To observe the changes of SOD and MDA after the rabbits were exposed to acute hypoxia when cured by oxygen therapy.Methods New Zealand rabbits underwent hypoxia imitating the 4500-meter sea level in altitude chamber(group E,n=6),then some received oxygen therapy,the volume ratio of oxygen-nitrogen mixture was 25%,30% and 35% respectively for 10,5 and 3 hours as group B,C and D.Another 5 rabbits received no treatment as normal group.The rabbits samples were collected at the time points of 1,3,5,10 and 24 hours after the oxygen therapy started to detect serum biochemical indicators.Results Comparing with normal control group,serum SOD activity in simple hypoxia group noticeably decreased and the content of MDA noticeably increased;in three oxygen therapy groups,serum SOD activity in 25% oxygen therapy group was highest and the content of MDA was lowest.Serum SOD activity in 25% oxygen therapy group had ascendant tendency and the content of MDA had descendant tendency.Conclusions Low concentration oxygen therapy can prevent acute hypoxia-induced injury in rabbits,besides increase oxygen partial pressure,oxygen therapy may improve superoxide dismutase activity.

BACKGROUND: Chitosan/poly (3-hydroxybutyrate) (PHB) copolymer has been paid close attention for special biological source of Chitosan and PHB. However, there has been no proper method for them to polymerize effectively.OBJECTIVE: To prepare chitosan/PHB graft copolymer in a homogeneous medium, using a gentle initiator.DESIGN, TIME and SETTING: This study, a single-sample experiment, was performed at the Research Center of Chemistry, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province, China from August 2007 to October 2007.MATERIALS: Chitosan: DD=100%, Mη=123000, Kyoto, Japan. PHB was purchased from Aldrich chemicals and the molecular weight was 10000.METHODS: Chitosan was grafted with poly (3-hydroxybutyrate) (PHB) in acetic acid/dimethyl sulfoxide system, and benzoyl peroxide (BPO) was used as initiator. The reaction temperature was 85℃ and the reaction continued for 5 hours with nitrogen protection. Grafting reaction and the chemical structure of the copolymer were confirmed by infrared analysis, NMR and elemental analysis. The crystal form and thermal stability of copolymer were characterized with wide-angel X-ray diffraction (WAXD) and thermogravimetric/differential thermal analysis balance, respectively.MAIN OUTCOME MEASURES: The chemical structure of copolymer, crystal form as well as thermal stability.RESULTS: Grafting reaction was confirmed by infrared analysis, NMR and elemental analysis. Wide angle X-ray diffraction and thermogravimetric analysis indicated that graft copolymer was different from chitosan and PHB in crystalline morphology, and had a good thermal stability.CONCLUSION: Using BPO as initiator, chitosan/PHB grafting copolymer is prepared and it has a steady property.