Objective To study the histocompatibility of domestic new type nitinol alloy ventricular septal defect(VSD) occluder in order to test the feasibility for clinical application.Methods The domestic new type nitinol alloy VSD occluder was inserted into 20 healthy dogs with intravenous balanced anesthesia and tracheal intubation,assisted by animal breathing machine respiration.The dogs were given oral antibiotic and would be killed in several months after operation.According to different slaughter time(1,2,3,6 months),they were divided into 4 sections and including 5 dogs in each section.The occluder sites,the intimal coverage and the change of inflammatory cells were observed through gross anatomy,with the help of the light microscope and electron microscope.SPSS 11.0 software was used to analyze the data.Results The thickness of the endocardial enhanced with the time(P

Effect and safety of repeated embryo aspiration for reduction of muhifetal cervical pregnancy Was retrospectively evaluated.Three women with muhifetal cervical pregnancy successfully received conservative treatment of repeated embryo aspiration under the guidance of transvaginal ultrasound,and the gestational sac contents were aspirated completely.Local(2.5～5.0 mg injectable methotrexate,MTX) or systemic medication was used.Our findings showed that repeated embryo aspiration for reduction of cervical multifetal pregnancy could improve the coupe of disease,decrease systemic medication of MTX,and discontinue a cervical pregnancy within 8 weeks effectively and safely.However,the mminimum therapeutic dose of MTX remained to be explored.

Objective To analyze the clinical effects of sevoflurane and propofol anesthesia on hemorrheology in elderly patients with colorectal cancer undergoing radical mastectomy.Methods 86 patients with colorectal cancer undergoing radical surgery in our hospital from March 2014 to December 2016 were selected and randomly divided into sevoflurane group and propofol group group with 43 cases in each group.Two groups of elderly patients were anesthetized before, 40 minutes after induction, 80 minutes after induction, 140 minutes after induction and 30 minutes after entering the anesthesia monitoring room 30 minutes to draw the neck 4 mL venous blood.Analysised of two groups of patients with hemorheology ( low cut blood viscosity, blood viscosity in the cut, high shear whole blood viscosity, plasma viscosity, erythrocyte deformation index, erythrocyte aggregation index) index.Results There was no significant difference in the difference of detection indexes between sevoflurane group and propofol group at each time point Comparison of sevoflurane group and propofol group in elderly patients, 40 min, 80 min after induction and 140 min after induction compared with those of pre-anesthesia low-cut whole blood viscosity, middle whole blood viscosity, high shear whole blood viscosity and plasma viscosity, the difference was statistically significant(P<0.05), erythrocyte deformation index and erythrocyte agglutination index were not significantly different between the two groups.The blood viscosity in the propofol group was significantly lower than that in the anesthesia group at 80 minutes after induction and 140 minutes after induction, the difference between the two groups was statistically significant (P<0.05).The whole blood viscosity of the sevoflurane group was significantly lower than that before anesthesia at 140 min, and the difference between the two groups was statistically significant (P<0.05).Conclusion From the point of view of hemorheology, sevoflurane and propofol anesthesia can be used in elderly patients with colorectal cancer radical operation.

Objective To investigate the effects of cinnamaldehyde and citral on DNA and RNA of Aspergillus flavus and A. fumigatus cells and their mechanisms. Methods A. flavus and A. fumigatus were incubated on Czapeks agar plate (treated with cinnamaldehyde and citral at different concentrations) at 26.5 ℃ for 3—6 d. The normal and treated cells were observed by laser scanning confocal microscope (LSCM) and image analysis to describe the DNA and RNA levels by quantity and localization. Results DNA and RNA levels were changed greatly and multinucleate coniospores appeared in the treated cells. Conclusion Cinnamaldehyde and citral have directly or indirectly interfered the conventional synthesis of fungal hereditary DNA and RNA and normal differentiation of conidiophore in A. flavus and A. fumigatus, thus inhibiting the normal cell cycle and the growth and propagation of fungi.

Objective To investigate the effect of half-body ionizing radiation on the concentration of MDA, SOD in serum and the spleen index, CFU-S and the protective function of supplementary taurine (Tau) in half-body ionizing irradiated mice. Methods Kunming mice were randomly divided into 6 groups: normal control, total-body irradiation (TBI), total-body irradiation+Tau, left-half-body irradiation, half-body irradiation+Tau, and total-body shielding irradiation. The relevant indexes such as the spleen index, spleen colony forming units (CFU-S) were observed. The ionizing radiation was performed under ~ 60 Co ? ray with absorbed dose 8.0 Gy, dose rate 68.46 cGy/min. Taurine at dose of 500 mg/kg was injected intraperitoneally twice a day before irradiation, once 30 min before irradiation, and once 6 h after irradiation. Results In TBI group, the spleen index and CFU-S were decreased remarkably, MDA increased and SOD reduced in serum. The hematopoietic function of spleen was not improved by supplementary taurine (P

Objective To investigate the value of high-frequency ultrasound in diagnosis of thyroid carcinoma by comparative analysis of high-frequency ultrasound and spiral CT imaging results. Methods The imaging results of patients with thyroid carcinoma proved by postoperative pathology or biopsy results were reviewed.High-frequency ultrasound and spiral CT were used to examine the 35 patients in The Tumor Hospital of Harbin Medical University between 2007 and 2009. Results Diagnosis of thyroid carcinoma by application of Highfrequency ultrasound were 27 cases, diagnosis rate was 77.1%(27/35);by spiral CT were 25 cases, diagnosis rate was 71.4% (25/35);comparison of the two methods showed no significant difference (x2= 0.3, P ＞ 0.05). Combined application of high-frequency ultrasound and spiral CT diagnosed 33 patients with thyroid carcinoma, diagnosis rate was 94.3%(33/35), which was significantly higher than that of high-frequency ultrasound alone or that of spiral CT alone(compared with high-frequency ultrasound, x2 = 4.2, P ＜ 0.05;and spiral CT, x2 = 6.4, P ＜ 0.05). Conclusion Combined application of high-frequency ultrasound and spiral CT can improve the diagnosis rate of thyroid carcinoma.

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Objective To evaluate effects of human papilloma virus(HPV)16E7 on expressions of six tumor suppressor genes(including MT1G, NMES1, RRAD, SFRP1, SPARC and TFPI2)in a cell line SiHa, as well as on its proliferation and apoptosis. Methods SiHa cells were divided into two groups to be transfected with a small interfering RNA targeting HPV16E7(E7SiRNA, experimental group)and an empty vehicle(negative control group) respectively, with SiHa cells receiving no treatment serving as the blank control group. After 48 hours, qPCR was performed to measure the mRNA expressions of E7 and six tumor suppressor genes, CCK?8 assay to evaluate cellular proliferative activity, and flow cytometry to assess apoptosis of SiHa cells. Results At 48 hours after the transfection, the experimental group showed significantly decreased E7 mRNA expression(0.25 ± 0.036, P<0.05), but increased mRNA expressions of the six genes(MT1G 1.403 ± 0.190, NMES1 1.720 ± 0.060, RRAD 1.390 ± 0.160, SFRP1 1.493 ± 0.120, SPARC 2.157 ± 0.144, TFPI2 2.060 ± 0.122, all P < 0.05). The proliferative activity of SiHa cells was significantly decreased(0.554 ± 0.130 vs. 1.028 ± 0.236 and 1.220 ± 0.126, both P<0.05), but the apoptosis rate was significantly increased(9.222%vs. 0.246%and 0.123%, both P<0.05)in the experimental group compared with the negative control group and blank control group. No significant differences were observed between the negative control group and blank control group in proliferative activity or apoptosis rate of SiHa cells(both P>0.05). Conclusion E7 may participate in HPV16?induced cellular malignant transformation by suppressing the mRNA expressions of 6 tumor suppressor genes, including MT1G, NMES1, RRAD, SFRP1, SPAR and TFPI2.