Ceramides ; Fatty Liver, Alcoholic ; Humans

Drug Resistance, Viral ; Endopeptidases ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; virology ; Humans ; Interferons ; pharmacology

Objective To investigate the protective effects of ulinastatin on the hearts of rats with anoxia-induced cardiac arrest-cardiopulmonary resuscitation(CA-CPR) and the mechanism of improving cardiac dysfunction .Methods Twenty male Sprague Dawley(SD) rats were randomly divided into three experimental groups :sham operation group (group A ,n= 8 ,only anesthesia , tracheotomy tube and vascular puncture) ,control group(group B ,n= 6 ,normal saline 4 mL · kg -1 injected via vein) ,Ulinastatin treatment group(group C ,n=6 ulinastatin 50 000 U/kg+normal saline 3 mL · kg -1 injected via vein);Factors including mean arte-rial pressure(MAP) ,left ventricular end diastolic pressure(LVEDP) ,the maximum rising and falling rates of left ventricular deep pressure(± LVdp .dt-1max) ,brain natriuretic peptide(BNP) ,cardiac troponin T(cTNT) ,IL-12 and TNF-αwere observed at setting time before and after cardiopulmonary resuscitation in rats .Results Compared with those of the group A and before CA-CPR ,the concentrations of IL-12、cTNT、TNF-α、BNP、and LVEDP increased(P<0 .01)while ± LVdp .dt-1max decreased(P<0 .01) at 6 h after CA-CPR in group B ,C .Compared with those of group B ,the concentrations of IL-12、CTNT、TNF-α、BNP and LVEDP of 6 h after CA-CPR in group C were lower and ± LVdp .dt-1max was higher(P<0 .01) ,The concentrations of MAP of 6 h after CA-CPR in group B was lower Compared with that of group A ,C and before CA-CPR(P<0 .01) .Conclusion Ulinastatin can improve cardiac dysfunction by depressing mediators of inflammation and reducing myocardial injury .

Objective To evaluate the safety of nasogastric tube feeding and nasojejunal tube feeding in early enteral nutrition treatment of acute pancreatitis.Methods Using key words,subject headings,and citation tracing,we searched literatures reporting randomised controlled trials on early enteral nutrition treatment of acute pancreatitis through nasojejunal tube and nasogastric tube in the following databases:PubMed,Embase,Cochrane library,Wanfang,China National Knowledge Infrastructure (CNKI),and VIP published since the founding of the databases up to 2016.Meta-analysis was performed with the selected literature.Results Seven randomised controlled trials with 367 patients were included.Meta-analysis showed that the nasogastric tube group was not inferior to the nasojejunal tube group in the incidence of recurrent abdominal pain,gastrointestinal adverse reaction,the total length of hospital stay,and mortality.Conclusion Enteral nutrition via nasogastric tube is safe and well tolerated,may be a safe approach of nutrition treatment for acute pancreatitis.

Objective To investigate the in-vitro transdermal permeability of cyclovirobuxine D ethosomes.Methods Ethosomes,liposomes,saturated aqueous solution and 35.5% saturated alcohol solution of cyclovirobuxine D were prepared.The in-vitro transdermal permeability of different kinds of preparations of cyclovirobuxine D was studied by in-vitro permeation experiments.Results Cyclovirobuxine D ethosomes enhanced transdermal flux rate of cyclovirobuxine D and the enhancement ratio was 8.85,superior to liposomes and saturate alcohol.The dermatic hold-up amount of cyclovirobuxine D in 35.5% saturated alcohol solution,liposomes and ethosomes was in a decreasing sequence.Conclusion Compared with the liposomes,the ethosomes can enhance the transdermal flux rate of cyclovirobuxine D,which could increase the systematic action of the drug.

Objective To study the effect of Atorvastatin on the level of serum high-sensitivity C-reactive protein(hs-CRP) and nitric oxide(NO) in patients with acute cerebral infartion(ACI). Methods 60 patients with ACI were randomly divided into the Atorvastatin therapy group (n=30) and the conventional therapy group (n=30). At the basic of conventional therapy, the Atorvastatin therapy group was treated with Atorvastatin 20 mg/d. Both groups were treated for 14 consecutive days. The levels of serum hs-CRP and NO were measured and the scores of neurological deficit (NDS) were evaluated before and after treatment. Results The levels of serum hs-CRP in both two groups after 14 d treatment were significantly lower than those before treatment ( all P

Objective To evaluate the effect of pantoprazole and norepinephrine injection on serum levels of inflammatory factors and hemostasis in the treatment of patients with peptic ulcer bleeding .Methods 102 cases of patients with peptic ulcer bleeding from September 2014 to September 2016 in our hospital were selected and randomly divided into observation group ( n =51 ) and control group ( n =51 ) .Observation group were received pantoprazole and norepinephrine injection combination therapy, the control group received only pantoprazole therapy.The treatment lasted 3 days.The efficacy, bleeding time, hospitalization and blood transfusion, serum IL-6 and IL-10 levels, the incidence of rebleeding and incidence of adverse reactions were compared between two groups.Results The overall response rate ( 98.02%) was significantly higher ( 82.35%) ( P<0.05 );observation group bleeding time was significantly faster than the control group ( P<0.05 ) , length of hospital stay was significantly shorter than the control group (P<0.05), blood transfusion was significantly less than the control group (P<0.05); levels of serum IL-6, IL-10 after treatment were lower than those before treatment (P<0.05); the serum levels of IL-6, IL-10 after treatment were significantly lower than those in control group (P<0.05); rebleeding rate in observation group was significantly lower than that in control group (P<0.05);there was no significant difference in adverse reactions between two groups.Conclusion Pantoprazole and norepinephrine injection in the treatment of digestive bleeding ulcer bleeding has the obvious effect, can reduce serum levels of inflammatory factors, while also reduce the incidence of rebleeding, safe and reliable.

Objective To summarize the experiences in diagnosis and treatment of thymoma in 120 cases. Methods 120 patients with thymoma were operated on, and the diagnosis was confirmed pathologically, from January 1994 to December 2003 in our department. According to Masaoka clinical and pathological classification, 54 cases were stage Ⅰ, 32 were stage Ⅱ, 22 were stage Ⅲ, 12 were stage Ⅳ. In 42 cases there were symptoms of myasthenia gravis. Result Total excision was performed in 112 cases and partial excision in 8 cases. When the tumor was beyond stage Ⅱ, postoperative radiotherapy was given. Recurrence occurred in 5 patients 1-7 years after operation. Conclusions Surgical resection is currently the main therapeutic method for tumor of the thymus. The preferred surgical approach was excision of thymoma and removal of perithymic fat in the anterior mediastinum through a median sternotomy. Thymoma is a potentially malignant disease, and the patients with the tumor beyond stage Ⅰ must undergo postoperative radiotherapy. Local recurrence of the tumor should be surgically excised.

Objective To compare the levels of serum CEA, NSE and CYFRA21-1 of patients with lung cancer and those with benign lung diseases, and to evaluate the clinical differential diagnostic value of combined detection of serum CEA, NSE and CYFRA21-1 in patients with lung cancer. Methods CEA, NSE and CYFRA21-1 were determined with enzyme-linked immunosorbent assay in 122 patients with lung cancer and 37 patients with benign lung diseases. The data was analyzed by t test. Results The serum levels of CEA(12.95?33.04ng/ml), CYFRA21-1(4.87?0.638ng/ml) in patients with lung cancer were significantly higher than that in those with benign lung diseases (1.92?0.84ng/ml and 4.87?0.638ng/ml, respectively)(P0.05). The levels of serum CEA, NSE and CYFRA21-1 were related to types of pathology. The highest levels of CEA, NSE and CYFRA21-1 appeared in adenocarcinoma, small cell carcinoma and squamous cell carcinoma, respectively. The level of serum of NSE (26.05?20.69ng/ml) in patients with small cell carcinoma was significantly higher than that of the patients with benign lung disease, adenocarcinoma (11.26?6.97ng/ml) and squamous cell carcinoma (12.71?7.64ng/ml), respectively (P0.05). Conclusion Combined determination of serum levels of CEA, NSE and CYFRA21-1 is helpful in differential diagnosis of lung cancers.

The serum level of intravenously dripped phenytoin was deter- mined in rabbits before and in different time intervals of a week after intramuscular injection of diazepam with fluorescence polarization immunoassay and the parameters were calculated on a microcomputer.It was found that in the 12th hour after diazepam injection,the serum level of phenytoin was significantly different from that of the control,and t1/2 of phenytoin was significantly prolonged;the model of pharmacokinetics of phenytoin was changed from one compartment open model to two compartment open model.Yet the AUC and Cl of phenytoin were not markedly altered.