Objective To understand the death level and main death causes among residents in the Wansheng Economic De‐velopment Zone to provide the basis for comprehensive prevention and control .Methods According to the ICD‐10 international classification of diseases ,the death causes were classified and coded for statistically analyzing the crude mortality rate ,standardized mortality rate and the death causes constituents in this surveyed population .Results The crude mortality rate of residents in Wan‐sheng Economic Development Zone during 2014 was 650 .57/100 000 and the standardized mortality rate was 413 .35/100 000 .The sequence of death causes was circulatory system disease ,respiratory system disease ,tumor ,injury and poisoning and digestive sys‐tem diseases .The death number for these 5 kinds of death causes accounted for 93 .21% of the total death number .The top five of potential life loss years were injury and poisoning ,tumor ,circulatory system disease ,respiratory system disease and digestive system diseases .The rates of potential years of life loss were 13 .76‰ ,10 .19‰ ,8 .35‰ ,4 .84‰ and 2 .69‰ respectively .Conclusion Chronic noncommunicable diseases ,injury and poisoning become the main causes of death in the Wansheng Economic Development Zone and disease burden .

Bone Marrow Mesenchymal Stem Cells (BMSCs) are ideal seed cells for cell therapy,which have been through many long-term studies of in vitro and animal disease model treatment and have been widely used in preventing and treating a variety of clinical diseases caused by aging and other damage,including bone injury and regeneration,neural degenerative and injury,myocardial injury,liver cirrhosis,diabetes,lung disease and so on.BMSCs application in the treatment of orthopaedic disease is the most common and effective.By retrospective literature review,we summarize the BMSCs transplantation application in Orthopaedic disease treatment.As an ideal cell source,BMSCs was first applied in bone tissue engineering,mainly used in the treatment of long bone defect and bone nonunion;Recently,the research trends of clinical BMSCs application turned to treat degenerative diseases and genetic disorders because of its great potential,including osteoarthrits,femoral head necrosis,disc degeneration,spinal cord injury,knee varus,osteogenesis hypoplasia,hypophosphatasia and so on.The safety and efficacy of BMSCs treatment are the key issues in clinical cell therapy.There is no side effects and complications after BMSCs treatment,prove its clinical application is safe.But due to type and degree of disease and individual differences,therapeutic methods including injection method,effective concentrations,treatment window selection,cycle and therapeatic effects have a bigger difference.There is still no uniform of recognized operation standards for the treatment of the same type of disease.So BMSCs transplantation requires effective control during whole medical process and its application in orthopedic diseases is still lack of large-scale multicenter clinical study.Therefore,this review focuses on induction,summary and analysis in the research status of BMSCs in bone disease treatment,and provide new ideas and methods for BMSCs transplantation in orthopaedic disease prevention and treatment.

Colorectal cancer is a common malignant tumor of digestive tract, and the risk of inflammatory bowel disease developing into colorectal cancer is significantly increased. Immune signaling pathways NF-κB, IL-6/STAT3, COX-2/PGE2, IL-23/Th17 and TLRs have been confirmed to promote the transformation from colitis to colorectal cancer. NOD2 and intestinal microbes also participate in the regulation of inflammation mediated carcinogenesis. Chronic inflammation is a potential risk for colorectal cancer, and anti-inflammatory drugs may play a chemical preventive role. In this review, we summarize the signaling pathways involved in inflammation-associated colon carcinogenesis and evaluate the chemoprophylaxis of colon cancer.

OBJECTIVETo evaluate the clinical effects of arthroscopic reconstruction of anterior cruciate ligament (ACL) and minimally invasive reconstruction of posteromedial corner (PMC).

METHODSThere were 22 cases of ACL and PMC tear were performed with reconstruction from March 2012 to February 2014. The patients were 29.4 years old on average, including 8 males and 14 females. ACL reconstruction was performed under arthroscopy and PMC reconstruction was performed minimally invasively through the ACL incision. The stability of knee was assessed by anterior drawer test,Lachman test,vulgus stress test and Slocum test. The function of knee was assessed by Lysholm score and Tegner activity rating. MRI of knee was checked 12 months after operation.

RESULTSThe stability tests of all patients were negative at 2 and 6 months after operation, and there was one positive case in anterior drawer test and another positive case in vulgus stress test at 12 months after operation. Lysholm score of all patients 12 months after operation was 96.8 +/- 6.8, which was significantly better than 32.0 +/- 11.2 before operation. Tegner activity rating of all patients at 12 months postoperatively was 6.1 +/- 0.9, which was significantly better than 0.9 +/- 0.5 before operation. It showed the grafts were very well in the MRI 12 months postoperatively.

CONCLUSIONArthroscopic ACL reconstruction and minimally invasive PMC reconstruction can restore the stability of knee.

Adult ; Anterior Cruciate Ligament ; physiopathology ; surgery ; Anterior Cruciate Ligament Injuries ; Anterior Cruciate Ligament Reconstruction ; Female ; Humans ; Knee Injuries ; physiopathology ; surgery ; Knee Joint ; physiopathology ; surgery ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; Treatment Outcome ; Young Adult

Objective To investigate the effectsof quercetin on human umbilical vein endothelial cell (HUVEC)-12 undergoing endothelial-to-mesenchymal transition (EndMT) induced by TGF-β1; To discuss its mechanism of action. Methods Cell activity of intervening by quercetin with different concentrations and TGF-β1 for 72 h was detected by CCK-8 method;RT-PCR and immunofluorescence staining were used to detect the transition of endothelial and stromal markers; Western blot was used to detect the signal transduction pathway; RT-PCR was performed to detect the transcription factors that play crucial roles in the process of transformation. Results The results of RT-PCR and immunofluorescence double staining showed that TGF-β1 (10 ng/mL) stimulated HUVEC-12 cells for 72 h to induce fibroblast phenotype, showing more interstitial markers and less endothelial markers;Western blot and RT-PCR results showed that quercetin inhibited the phosphorylation of smad2/3 in a concentration-dependent manner;After TGF-β1 stimulation, the downstream transcription factors EndMT of snail1, twist1, twist2, ZEB1, and ZEB2 significantly increased, while 100 μmol/L quercetin could down-regulate the five downstream transcription factors. Conclusion Quercetin has anti-fibrosis effects through inhibiting HUVEC-12 cells undergoing EndMT.

Objective To investigate the strategy and announcements of surgery in primary retroperitoneal. tumor for improving the rescetable rate and decreasing the surgical risk.Methods Clinical data of 50 cases undergoing surgery of retroperitoneal tumor in our hospital from January 2004 to December 2008 were retrospectively analyzed.Results Tumors were benign in 20 cases and malignant in 30 cages.Complete resection was achieved in 39 cases(78%).in which combined organ resection carried out in 10 cases(20%),3 cases(6%)underwent partial resection.non-resection and had biopsy only were performed in 8 cases(16%),so the rescetable rate of this clinical weTe 84%(42/50).Conclusions Abundant preoperative preparation,wide open operation field and distinct anatomical dissection were the essential condition of the resecting primary retroperitoneal tumor.The relevant surgical skill should decrease the surgical risk.The tumor should be completely reaeeted in principle,and invaded neighboring organs should be resected with the discretion at the gane time.Completely resected was the key 10 improving the surviral rate and the strategy to decreasing the reeurence rate for recurrent retroperitoneal tumor.

Objective To study the cytotoxicity of the membrane material of heart assist pump. Methods The membrane materials tested incladed polyurethane (group A) and medical polychloroethylene (group B),and a blank control group with none of the material. L-929 cells (the mouse fibroblasts) were cultured on either material in groups A and B,and 6 cell cultures were without any material. After 24,48 and 72 hours,the growth of cell in groups A,B and the control was observed,and the number was counted. Educts of two materials used in groups A and B was respectively added to culture medium,then the OD was measured every 24h and proliferation rate was determined by MTT test. Results L-929 cells grew very well in groups A and B with less death. The cell counts showed no difference between 2 groups at each time. The OD of group A was always higher than that of group B after 24,48 and 72h ( P

Objective To explore the mechanism of therapeutic effect of Yichantang in the treatment of Parkinson′s disease (PD). Methods A rat model of PD was replicated. The experimental animals were divided into a normal control group,a normal saline control group and a Yichantang group. Changes in the rats' behavior pattern was obrerved. Using the technique of intracerebral dialysis,the content of catecholamine like substances in the nigral extracellular fluid(NECF)was measured by means of high performance liquid chromatography(HPLC). Changes in the affinity and quantity of M and DA receptors were determined too. Results Yichantang markedly improved the rats' rotation behavior,and enhanced the Bmax and KD of DA and M receptors in the brain tissues on the side of lesion. The levels of DOPAC,HVA,DA and 5-HT in the NECF of the side of lesion in the Yichantang group were significantly higher than that of the normal saline group ( P

280r/40min). So the PD model was regarded eligible. In the site of lesion in SN, the number and volume of dobaminergic neurons were decreased, and the tissue structure of SN became indistinct, the levels of dopamine (DA), dihydroxyphenylaceticacid (DOPAC), homovanillicacid (HVA) 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleaceticacid (5-HIAA) were more conspicuously reduced than that in uninjured side. TH-immunoreactive cells of substantia nigra compact part were lessened obviously on injured side. The abnormal rotatory behavior of PD rats did not spontaneously disappear during 10 months of continuous observation. Conclusion The PD rat model, which is reproduced by injecting 6-OHDA to selectively destroy dopaminergic neurons in the substantia nigra of rat, is stable and reliable, and demonstrated similar primary pathological changes of PD.

Objective To investigate the diagnostic value of low field MRI for adenomyosis. Methods MRI features of adenomyosis pathologically proved in 18 cases were retrospectively analysed.Results 15 cases were diffusive adenomyosis,the junctional zones of uterus were exteusive thickened to 12.0～32.6 mm,mean 16.2 mm,diffusive high signal intensity distributed over in myometrium which was low signal intensity in 11 cases,it was typical “snowing sign”,lower signal intensity in the myometrium in another 4 cases on T 2WI and fat-suppression imaging. A little high signal intensity was found in 6 cases on T 1WI. 3 cases were focal adenomyosis(adenomyoma), 4 lesions totally. The adenomyoma’s boundaries were not distinct and their shapes were roundish or irregular. The lesions were low signal intensity or diffusive high signal intensity distributed in the low signal intensity fields on T 2WI and fat-suppression imaging. A little high signal intensity was found in 2 lesions on T 1WI. Conclusion T 2WI and fat-suppression imaging of low field MRI are very useful techniques of the diagnosis of adenomyosis.