Objective To explore the sub-health and TCM physical constitution of stroke patients’families.Method Four hundred and twelve family members of stroke patients hospitalized during September 2001 to May 2013 were involved in the survey by filling out TCM physical constitution scale(TCMPC)and the sub-health self-rating scale(SSS)for the purpose of investigating the sub-health and conditions of TCM physical constitution.Results Of all the patients,189 were in sub-health,accounting for 45.87%. There were 7 main clinical symptoms related to sub-health,including poor sleep,less activeness,proneness to upsets and irritation, memory loss,depression,tension in interpersonal relations and appetite loss,with the incidences ranging from 64.55%to 48.15%. In TCM,51.85%of the family members were balanced in physical constitution,10.58%of them had Yang-deficiency constitution, 14.29%of them had Yin-deficiency,23.28%of them other kind of constitution.Conclusions The family members of stroke patients have a high rate of sub-health.The members in sub-health hold a balanced constitution,Yang-deficiency or Yin deficiency.In order to eliminate their sub-health,improving the quality of care for patients with stroke,we should guide the families of stroke patients to develop good living habits,strengthen the TCM mental nursing,help them to establish a good social support system.

The toxic effects of immunosuppressants are often dangerous factors which influence long-term survival of recipients.Individualized immunosuppressive therapy has been a hotspot and difficulty for comprehensive treatment of liver transplantation.Immunosuppressive therapy has been gradually developed to pursue long-term survival for receipients and graft,minimized side effects of immunosuppressants,optimized quality of life of patients,and reduce the economic burden of patients.Through consideration of patient's pathophysiological state,understanding the characteristics and side effects of various immunosuppressive,appropriate choose of the immune inhibitor,correct evaluation of immune state of recipients and graft,minimization of drug doses,individualized dosing regimen could be successfully carried out.

Objective To investigate the significance of clinical nursing pathway in perioperative period of children with hypospadia.Methods 63 cases of hypospadia children patients were randomly divided into the control group (30 cases)and the experimental group (33 cases).The traditional nursing method was used in the control group,while the clinical nursing pathway was used in the experimental group.Results The hospital stay,hospital charges and complications of the experimental group were less than the control group.The satisfaction degree of the experimental group was higher than the control group.There was significant difference.Conclusions Clinical nursing pathway is a safe and effective nursing model.It can shorten hospital stay,reduce hospital charges,decrease complications,and improve patients' satisfaction degree.

Objective To screen and identify protein interacted with enterovirus 71 (EV71)3A protein by means of T7-phage display system.Methods The prokaryotic expression vector of 3A protein was constructed for expressing and purifying 3A pro-tein.With 3A protein as the target protein,the T7-phage display system was adopted to screen the human hepatocyte cDNA library. The screened products were performed the DNA sequence analysis and the homologous research.Results 3A protein was expressed and purified,after 4 rounds of biopanning,37 positive clones were selected.The inserted fragments were amplified by PCR using specific T7 primer.The products were sequenced,2 proteins were identified to interact with 3A protein by homologous analysis. Conclusion Trough the T7 phage display system,two proteins interacted with 3A are obtained.The possible function of 3A protein can be speculated by researching the function of these two proteins,which lays the foundation for the further studying the pathogen-ic mechanism of EV71.

The paper introduces the development status and requirements for informatization construction of pre-hospital first-aid in Nanning Emergency Medical Service Center,illustrates the pre-hospital first-aid informatization construction and management and analyzes the effects of pre-hospital first-aid informatization construction,including improving the pre-hospital first-aid ability,overall command and scheduling ability and first-aid response ability,etc.

Objective To measure the expressions of TXNIP,STAMP2 and GATA3 in diabetes patients with breast cancer and to determine the changes of oxidative stress indexes before and after surgery.Methods The expression levels of TXNIP,STAMP2 and GATA3 mRNA in cancer tissues and adjacent normal tissues were measured in 45 diabetes patients with breast cancer.The levels of serum oxidative stress indexes including MDA,MPO,SOD and TAC were detected and compared before and after surgery.Results The expressions of TXNIP,STAMP2 and GATA3 mRNA and their protein levels in cancer tissues were lower than those in adjacent normal tissues (P<0.05).The positive expression rate of the three indexes were 60%,66.7% and 73.3%,higher than that of TXNIP protein with 2.2% in the adjacent normal tissues(P<0.05).The TXNIP,STAMP2 and GATA3 protein positive rate of breast cancer tissue were related to differentiation,lymph node metastasis and clinical grades (P<0.05).Serum MDA and MPO levels increased first and then decreased.SOD and TAC showed a trend of decrease first and then increase.The turning point is 6 d after surgery.Conclusion TXNIP,STAMP2,GATA3 and serum oxidative stress indicators may be the indicators for diagnosis and treatment of breast cancer in diabetes patients.

OBJECTIVE: To put forward proposals for improving essential medicines system in China.METHODS: The problems existing in the essential medicine system in China and the successful experience of some other countries were analyzed.RESULTS & CONCLUSION: Considering the current medical economy level and the capacity on research & development(R&D) of drugs,China should draw inspiration from WHO and other developed countries about their successful experiences on essential drugs to improve the screening methods for essential medicines list,assign legal status for essential medicines and build up system of production,procurement and distribution of essential medicines according to the basic national situation of our country and finally establish the essential medicine system suitable to our country's condition.

OBJECTIVE:To study the effect of breviscapine injection on glial fibrillary acidic protein (GFAP) and nitricoxide synthase (NOS) in rats with ischemical reperfusion(I-R)injury. METHODS:The rats were divided into 5 groups:sham group,I-R group,MgSO4 group,breviscapine low dose group and high dose group. Cerebral I-R injury model was established with suture method.The effect of breviscapine injection on GFAP and NOS were evaluated. RESULTS:In ischemia-reperfusion group compared with the sham group,the GFAP staining was strongly positive,serum NOS and inducible NOS (iNOS) activity were significantly enhanced(P

OBJECTIVE:To study the effect of breviscapine on SOD(superoxide dismutase)activity and apoptosis in rats after cerebral ischemia reperfusion(I-R).METHODS:The experimental rats were divided into 5 groups:sham group,I-R group,MgSO4 group,high dose breviscapine group and low dose breviscapine group.The SOD activities in brain tissue and serum of rats and the apoptosis of brain cells were observed.RESULTS:Compared with the sham group,the model group showed significantly decreased SOD activities in both brain tissue and serum(P

Objective To review the blood gas analysis of H7N9 flu patients for better understanding the characteristics and prognosis of the emerging disease.Methods A retrospective review was conducted for the 15 H7N9 influenza patients,whose blood gas profile was tested with a Radiometer 80 Blood Gas Analyzerin our hospital.The profile of blood gas analysis was compared between the 12 surviving patients and the 3 deaths in terms of their outcomes.Results The 3 patients who died of H7N9 influenza averaged 83 years old.The partial pressure of oxygen was <8 kPa in 9 of the 12 surviving patients,which meet the diagnostic criteria of type I respiratory failure.Their partial pressure of oxygen all recovered to normal (11 .033 ± 2.335 kPa,reference range 10-14 kPa)on discharge.The pH value of peripheral arterial bloold averaged 7.335 (range 7.39-7.54)on adission for the 3 patients who died of H7N9 influenza.The worst average pH value was 7.233 (range 7.32-7.35), which was significantly different from the values of the surviving patients.Conclusions Elderly patients are susceptible to H7N9 influenza infection and subsequent respiratory failure.Old age was associated with higher mortality.The partial pressure of ox-ygen is not significantly associated with patient outcomes.Appropriate active treatment may recover the blood gas profile to normal regardless of the initial values.If decreasing peripheral arterial bloold pH value is highly suggestive of poor prognosis, comprehensive measures should be taken to keep acid-base balance,including assisted ventilation to correct hypoxemia.