Objective To investigate on blood group serology of crossmatching incompatibility of blood donors in clinical hospi‐tals in Nanchong from 2010 to 2014 .Methods According to blood group serological test ,direct antiglobulin test (DAT ) was used for blood specimen with crossmatching incompatibility where donor was suspected as the causes ,at the same time irregular antibody was screened and identified .Results Among 77 returned blood specimens ,positive DAT were found in 43 cases ;while irregular an‐tibodies were found in 18 cases ,with anti‐M and anti‐E as the major ,followed by anti‐Lea and anti‐P1;and 16 cases did not find ab‐normality .Conclusion Causes of crossmatching incompatibility of blood donors in clinical hospitals in Nanchong are mastered through the survey ,to improve the safety level of clinical blood transfusion and avoid the waste of the blood .

Taking Cancer Hospital Affiliated to Southwest University as an example,the paper states the deployment of m-health network,and puts forward several wireless network optimization schemes such as wireless environment optimization,channel capacity improvement,code efficiency increase and user access restriction through clinical business traffic monitoring and data analysis.Through the result,this scheme can improve the performance of the wireless network effectively.

The paper presents a detailed review of British pharmaceutical standards including British Pharma-copoeia, European Pharmacopoeia, British Approved Names, Reference Standards and Non-Statutory Pharmaceutical Standards. British pharmaceutical standards are established by the British Pharmacopoeia Commission, Expert Advi-sory Groups, Panels of Experts and Working Parties, British Pharmacopoeia Commission Secretariat, British Pharma-copoeia Laboratory, and standard-setting procedures are clear and definite. British Pharmacopoeia standards are le-gally binding, and pharmaceutical products sold in the United Kingdom must comply with pharmacopoeia require-ments. British pharmaceutical standards can serve as an important reference for reforms to China’s pharmaceutical standards, including the scientific management of pharmaceutical standards, the improvement of organizational frame-works in the establishment pharmaceutical standards, the promptness of amendments to pharmacopoeia, and the uni-versality of international exchanges.

The paper analyzed the compensation mechanism applied in public hospitals in different localities in China,which are currently experimenting with the zero price margin for drugs.It also introduced the revenue variations resulting from this practice.It is discovered that public hospitals can hardly make their ends meet with merely governmental financial input,and that lowered income of doctors and price distortion arc evident in those experimental hospitals,with little progress in overcoming the subsidization of medical services with drug sales.The authors recommended a comprehensive reform,the mechanism of diversified health input,change of internal incentive mechanism within the hospital,improvement in the healthcare service pricing mechanism,and exploration of new operation mechanisms for public hospitals.

Objective To clone, locate and differentially analyze the transcription factor Relish gene from Anopheles stephensi, and to examine its signals-modulating action on prophenoloxidase cascade and melanization of Plasmodium yoelii oocysts. Methods Relish cDNA of total mosquitoes was amplified by RT-PCR with degenerated primers. Target PCR product was purified,cloned,sequenced and identified. Special Relish gene was amplified with specific primers from hemocytes or midgut,respectively. Semi-quantitative analysis was made under different feeding conditions. Relish message ribonucleic acid was identified with hybridization in situ. Results One cDNA segment of Relish similar to An.gambiae was acquired from An. stephensi. The same Relish gene was also manifested in the hemocytes and midgut. Marked up-regulation expression of Relish was observed at 6,12,24 or 48 h of Plasmodium yoelii infection and at 12 and 24 h after sucking nitroquine-acetate sucrose solution,that was before inducible oocyst melanization. Relish was also expressed in the hemocytes and midguts by ISH. Conclusion Transcription factor Relish of An. stephensi might play a role in signal modulation of Plasmodium yoelii infection and oocyst melanization.

Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg?L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg?L~(-1)(P

Nanoparticule (NP) as a drug and gene carrier has shown great potential and has been widely studied. Due to its ultra-small size, NP can achieve intelligent delivery of drugs, such as deliver drug site-specifically to disease focus or targeted tissue, even into target cells. The carrier materials of NPs provide it with such advantages as shielding odor, long-term sustained releasing of drug, lowering uoxicity,extend biologic half life of drug and gene, etc. This review emphasizes the manufacturing techniques and application of nanoparticles as drug and gene delivery system.

AIM:To develop a HPLC method for determing the contents of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills(Radix et Rhizoma Salviae miltiorrhizae,Fructus Schisandrae chinensis,Rizoma Acori tatarinowii,etc.). METHODS: The samples were extracted with methanol through supersonic wave and then filtered.The determination was performed by HPLC on Shim-pack VP-ODS C_(18) column using methanol-water-acetic acid glacial(65∶35∶0.1) as a mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 220 nm. RESULTS: The linear ranges of schizandrol,schisantherin and deoxyschizandrin were 0.001 2-(0.050 0) ?g(r=1.000 0)、0.001 4-0.057 6 ?g(r=0.999 5) and 0.002 1-0.060 8 ?g(r=0.999 8),respectively.The average recoveries of three components were 99.51%,99.40% and 99.06%,respectively.(CONCLUSION:)The method is accurate,quick,sensitive and can be used for measurement of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills.

There has been no consensus on the efficacy of extracranial-intracranial arterial bypass (EIAB) for ischemic cerebrovascular disease. As an important factor in the pathophysiologic mechanisms of ischemic cerebrovascular disease, the changes of cerebral hemodynamics have received attention. The article expounds the clinical significance of EIAB from the point of view of cerebral hemodynamics, particularly presents its preoperative assessment, operative procedures, and efficacy criterion.

Objective To compare the clinical use of continuous glucose monitoring system (CGMS) and self-monitoring blood glucose (SMBG) when monitoring blood glucose level of patients with gestational diabetes mellitus (GDM) or type 2 diabetes mellitus (DM) complicated with pregnancy.Methods A total of 99 patients with GDM (n=70) and type 2 DM complicated with pregnancy (n=29) that whether hospitalized or in clinical of Peking University First Hospital were recruited from Aug 2012 to Apr 2013.The CGMS was used to monitor their blood glucose level during the 72-hour time period,while the SMBG was also taken seven times daily.The correlation between these blood glucose levels and their glycosylated hemoglobin (HbA1c) levels were analyzed by comparing the average value,the maximum and the minimum value of blood glucose,and the appeared time of these extremum values in these two monitoring methods,and the amount of insulin usage was recorded as well.Results (1) The maximum,minimum and the average blood glucose value in the GDM group were (8.7± 1.2),(4.5 ±0.6) and (6.3 ± 0.6) mmol/L of SMBG vs.(10.1±1.7),(3.1±0.7),(6.0±0.6) mmol/L of CGMS.These values in DM group were (10.1±2.2),(4.5±1.0),(6.9±1.1) mmol/L of SMBG vs.(12.2±2.6),(2.8±0.8),(6.6±1.1) mmol/L of CGMS.By using the two methods,the maximum and the average value of the two groups showed significant differences (P＜0.01) while the minimum value showed no significant differences (P＞0.05).(2) In the GDM group,the average blood glucose values of CGMS and SMBG were significantly correlated (r=0.864,P＜0.01).The maximum values presented the same result (r=0.734,P＜0.01).Correlation was not found in the minimum values of CGMS and SMBG (r=0.138,P＞0.05).In the DM group,the average valves of two methods were significantly correlated (r=0.962,P＜0.01),the maximum values showed the same result (r=0.831,P＜0.01).It can also be observed in the minimum values (r=0.460,P＜0.05).(3) There was significant correlation between the average value of CGMS and HbA1c level (r=0.400,P＜0.01),and the average value of SMBG and HbA1c level were correlated (r=0.031,P＜0.05) in the GDM group; the average values of CGMS (r=0.695,P＜0.01) and SMBG (r=0.673,P＜0.01) were both significantly correlated with the HbA1c level in the DM group.(4) In the GDM group,37％ (26/70) of the minimum values of SMBG appeared 30 minutes before breakfast,while 34％(24/70) of them appeared 30 minutes before lunch; 86％(60/70) of the maximum values of SMBG were evenly distributed 2 hours after each of the three meals.In the DM group,41％(12/29) of the minimum values of SMBG presented 30 minutes before lunch,while 21％(6/29) and 14％(4/29) of them were showed 30 minutes before breakfast and dinner respectively; about 30％ of the maximum values of SMBG appeared 2 hours after each of the three meals.(5) In the GDM group,23％(16/70) of the minimum values of CGMS occurred between 0:00-2:59 am.,and most of the other minimum values of CGMS were evenly distributed in the rest of the day,except for 3％(2/70) of them were found during 18:00-20:59 pm.43％(30/70) of the maximum values of CGMS appeared during 6:00-8:59 am.,only 1％(1/70) and 3％(2/70) of them presented during 0:00-2:59 am.and 21:00-23:59 pm.,and the rest were evenly distributed for the other times of the day.In the DM group,34％(10/29) of the minimum values of CGMS were found during 0:00-2:59 am.,14％(4/29) of them appeared during 9:00-11:59 am.and 15:00-17:59 pm.,45％(13/29) of the maximum values of the CGMS presented during 6:00-8:59 am.,none was found during 21:00-23:59 pm.,0:00-2:59 am.and 3:00-5:59 am.,and the rest were evenly distributed for the other times of the day.(6) 64％(45/70) of the patients in the GDM group did not require for insulin treatment,while 36％(25/70) of them did.For those patients who received insulin treatment,after CGMS,64％(16/25) of them adjusted the insulin dosage according to their blood glucose levels.In the DM group,14％(4/29) of them did not receive insulin treatment,while for the others who did(86％,25/29); 60％ (15/25) of them adjusted the insulin dosage according to their blood glucose levels after CGMS.Conclusions Both CGMS and SMBG could correctly reflect patients' blood glucose levels.It was more difficult to control the blood glucose levels in patients with type 2 DM complicated with pregnancy than the GDM patients.Compared with SMBG,CGMS could detect postprandial hyperglycemia and nocturnal hypoglycemia more effectively.