Behcet Syndrome ; Child ; Humans ; Intestinal Diseases ; Male

Objective To investigate the effect of Astragalus Membranaceus( Ast) and Angelica Sinensis( Ang) on antithrombosis and explore the molecular mechanism. Methods Plasminogen activator inhibitor-1 ( PAI-1) expression and activity were selected to observe the antithrombosis effect of Ast and Ang ( 3 and 6 mg/ml) on 0. 5 ng/ml TGF-?1 treated endothelial cells. PAI-1mRNA expression was detected by RT-PCR,PAI-1 antigen assay was detected by specific enzyme-linked immunosorbent assays( ELISA) ,and PAI-1 activity was measured by amidolytical assay. Results Ast and Ang significantly inhibited PAI-1 mRNA expreesion,antigen and activity in endothelial cells. The more powerful effects could be observed when treated by Ast and Ang together. Conclusion The PAI-1 mRNA expression,antigen and activity is inhibited effectively by Ast and Ang,which may be the mechanism of their treatment for antithrombosis.

The paper analyzes the impact of MOOC on medical information resources construction,and proposes the response strategies for the information resources construction of medical library,including introducing Patron Driven Acquisitions (PDA) mode,strengthening digital resources construction efforts,developing medical MOOC course reference resources system and constructing medical library alliance.Meanwhile,it points out that the medical library is required to dig users'information demand further and solve the issue of MOOC copyright clearance.

OBJECTIVE: To determine and compare the contents of tanshinone Ⅱ A in difference dosage form of Guanxin danshen preparations(tablets,capsules,granules and dripping pills).METHODS: The tanshinone Ⅱ A in different Guanxin danshen preparations was extracted by ultrasonic extraction with methanol as solvent,and the content of tanshinone Ⅱ A was determined by HPLC on Agilent Zorbax C8(150 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-water(72∶28)under a detection wavelength of 270 nm.RESULTS: The linear ranges for tanshinone Ⅱ A stood at 2.76～138 ?g?mL-1(r=0.999 9).The average recoveries of Guanxin danshen tablets,Guanxin danshen capsules,Guanxin danshen granules and Guanxin danshen dripping pills were 99.29%,100.37%,100.18% and 98.71%,respectively with RSD at 1.78%,2.10%,1.55%,and 1.09%(all n=6),respectively.CONCLUSION:The content of tanshinone Ⅱ A in differed greatly in different Guanxin danshen preparations;therefore it is urgent to establish the quality standard for Guanxin danshen preparations as soon as possible.

OBJECTIVE:To provide reference for food & drug regulatory authorities in China to disclose government information.METHODS:In the light of the development and status quo of the disclosure of information in US FDA,the access,content and procedure for the disclosure of information in FDA were discussed.RESULTS & CONCLUSIONS:There are many similarities between US and China in the disclosure of information.In view of the practical situation of governmental information disclosure in food and drug regulatory authorities in China,Chinese government should be able to find out such key issues as the human and financial support is much needed in FDA information disclosure,the disclosure level should be in line with our national condition,a three-dimensional publicity system of government information at different level should be established and emphasis should be laid to the key disclosed information etc based on the research on the development,access,content and procedure of information disclosure in FDA.

Aim To investigate the inhibition mechanisms of baclofen, a specific GABA B receptor agonist, on quantal glutamate release in the rat spinal dorsal horn neurons.Methods Whole-cell voltage-clamp technique was performed on dorsal horn neurons in rat spinal cord slice to record glutamatergic spontaneous miniature excitatory postsynaptic currents (mEPSCs). Baclofen action on quantal glutamate release was assessed by analyzing the change of mEPESC to baclofen perfusion.Results Baclofen(10 ?mol?L -1,50 s) depressed the frequency, but not amplitude distribution of glutamatergic mEPSCs, indicating baclofen presynaptic depression on glutamate release. The depression on frequency of mEPSCs persisted in Ca 2+-free solution, or in the presence of K + conductance blocker, 4-AP. On the other hand, the depression was occluded by forskolin, an activator of adenylate cyclase, but not protein kinase C (PKC) activator phorbol 12,13-dibutyrate (PDBu). N-ethylmaleimide (NEM), a sulphydryl alkylating agent, which destroys G protein, abolished baclofen depression.Conclusion Not presynaptic K +, Ca 2+ conductance or PKC, but G protein and/or cAMP pathway are involved in the baclofen depression on glutamate release in rat spinal dorsal horn;this depression might contribute to the analgesic action of baclofen at spinal level.

Post-resuscitation multiple organ dysfunction syndrome(PR-MODS) is a major cause of low survival rate in patients with cardiac arrest.To fully understand the concept of PR-MODS is beneficial for estimating the state of illness,adjusting the therapy and eventually increasing the survival rate.This article reviews the development of PR-MODS concept and compares it with MODS of other causes,including their pathogeny,pathology,mechanism,diagnosis and therapy.

The blood gases and acid-base disorders were analysed in 276 cases with chronic cor pulmonale at 2260m above sea level (Xining). The results demonstrated that: (1) the incidence of acid-base disorders, respiratory acidosis and the ratio of compensatory to decompensatory respiratory acidosis were low, the rate of respiratory alkolosis was high, if they were evaluated according to the formula used on plains. If normal values determined in Xining were used, the various incidences were about the same as observed on plains. (2) In diagnosing respiratory failure with blood gas analysis if the criteria commonly employed on plains was applied, the incidence of type J respiratory failure was higher, and that of type I was lower. If PaO25.33kPa were used as the criteria for respiratory failue, the incidence of type I respiratory failure became lower with type Ⅱ higher. The characteristics of acid-base disorders and diagnostic criteria of respiratory failure in patients with cor pulmonale were discussed.

Objective To analyze the CT characteristic of pulmonary inflammatory mass and peripheral lung cancer, and to look for the method of diagnosis solitary pulmonary nodule. Methods 28 patients of pulmonary inflammatory masses and 33 patients of peripheral lung cancers were included in the study,all patients conformed by pathology. The lesions were divided into typical and atypical masses according to morphology. Observing the signs of lesions and using the results ameliorate the morphological classification. Tested the method by ROC. Results According to the standard of morphology, the diagnosis sensitivity, specificity and accuracy of inflammatory mass are 64.3%, 72.7% and 68.9% respectively. The ameliorated sensitivity, specificity and accuracy are 75%, 81.8% and 78 7% respectively. Conclusion The method of making morphology as basement and sign as supplement can improve the sensitivity, specificity and accuracy of diagnosis.

Just as "the market failures" in business or "the government failures" in government department, the non - profit organizations failures is displaying on kinds of forms. The reason that the non - profit organizations failed includ: seeking profit, the serious insufficiency of the organization funds, the morals out of control, the unscientific achievements evaluation system. The countermeasure settle of non - profit organizations failures include: to establish the information publishing system and the corresponding punishing system, perfecting the organization management system, forming the multiplicated financeing mechanism leading by government, perfecting the moral restraint mechanism which the autonomy and the heteronomy unifies.