Gastrointestinal bleeding is a severe,complicated and commonly seen disease in Department of Digestive Diseases,the frequent etiology is peptic ulcer,acute gastric mucosal lesion,esophageal gastric varices and digestive tract tumors. In recent years,clinicians gradually noticed a kind of disease characterized by acute,recrudescent and life-threatening bleeding,that is the non-variceal vascular originated gastrointestinal hemorrhage. The guidelines and scholars have not paid enough attention to this problem. However,non-variceal vascular originated gastrointestinal hemorrhage is not uncommon,and is difficult and tricky in its management for clinicians. Therefore,clinicians should pay sufficient attention to the characteristics and therapeutic principles of non-variceal vascular originated gastrointestinal hemorrhage.

P300/CBP is one of the most important high molecular weight protein histone acetyltransferase ( HAT) Although it is encoded by multiple different genes , P300/CBP is highly homologous , Because they have the similar amino acid sequence and functions ,and belong to the same class of proteins ,normolly they are all called P300/CBP.P300/CBP is involved in the activation of many kinds of transcription factors ,the protein itself alsohas acetyltransferase activity ,and is capable of acetylation of 4 core histones and transcription factor .More and more studies have confirmed the relationship of P 300/CBP variation withmultiple human diseases , including in-flammation,diabetes,heart disease and especially cancer .In tumor P300/CBP is associated with some pathways , and these pathways play a different roles in the tumor .Although P300/CBP is usually regarded as a tumor sup-pressor factor ,is plays different roles in different tumors ,This review mainly introduces the relationship of P 300/CBP with some solid tumor disease genes ,related transcription factors and their signaling pathways .

Objective To analyze the effect of CO2 laser under suspension laryngoscope on patients with vocal cord polyp. Method 118 patients with vocal cord polyp from November 2013 to August 2015 in our hospital were chose as research subjects, all patients were divided into observation group (n=54) and control group (n=64) accord﹣ing to different treatment. Control group: patients received conventional laryngoscope resection; observation group:patients received CO2 laser under suspension laryngoscope. Then compare the two group patients' voice indexes, a﹣cute phase proteins, rehabilitation and recovery speed after treatment. Results Observation group patients' F0, HNR values were higher than control group patients, PPQ, APQ, NNE values were lower than that in control group (P<0.05);early observation group patients' postoperative serum CRP, α1-AG, CER,β2-MG values were lower than con﹣trol group patients, ALB value was higher than control group patients (P< 0.05); observation group patients' cure rate was higher than control group patients, valid and invalid rates were lower than control group patients, the first sound time, sound full recovery time, discharge time were shorter than control group patients (P< 0.05). Conclusion Vocal cord polyp patients receive laser under suspension laryngoscope treatment, can effectively promote recovery rate, have advantages of minimally invasive and rapid recovery after surgery.

Of all types of intracranial hemorrhage (ICH) in the neonates, germinal matrix intraventricular hemorrhage(GM-IVH) in the premature infant is the most common type, mainly attributed to the existence of immature germinal matrix, lt's usually lack of visible and specific symptoms and signs in the nervous system, so its early and final diagnosis depends on the imaging examine. GM-IVH can cause periventricular hemorrhagic infarction, post-hemorrhagic hydrocephalus, periventricular leukomalacia and the associated cerebellar hemorrhagic injury, which are critical determinants of neonatal morbidity, mortality, and neurodevelopmental outcome in the survivors. The overall aim of this article is to review the current knowledge of the cause,mechanisms, imaging diagnosis, complication, management and outcome of GM-IVH in the preterm infant.

Many studies have demonstrated that blockade of angiogenesis by antiangiogenic drugs in di-fferent ways can control the growth of tumor.Antiangiogenic agents can target different sites:vascular growth pro-moting f_ators,endothelial cells,basement membrane degradation,epidermal growth factor receptor,ete.Antian-giogenic agents have promising prospect.

Radiotherapy is important in cancer treatment, but improving the therapeutic effect of irradiation and decreasing its toxicity to normal human tissues is still a global problem. Epidermal growth factor receptor (EGFR) is a member of ErbB family and is an important transmembrane receptor with signal-transduction tyrosine kinase activity. EGFR can direct cellular migration, adhesion, proliferation, differentiation, and apoptosis, and plays a fundamental role in the development and growth of many types of human tumor cells. A series of preclinical studies showed that EGFR inhibitors can enhance the antitumor activity of ionizing radiation. EGFR inhibi-tors regulate radio-sensitization through multiple mechanisms, including cell cycle alterations, DNA repair modulation, and anti-angio-genesis. Reasonable application of EGFR inhibitors will effectively increase the radio-therapeutic effect, extend the local control of tu-mor, and improve a patient's quality of life.

Objective To explore the clinical efficacy of rabeprazole combined with mosapride in the treatment of reflux esophagitis.MethodsThe clinical data of 315 patients with reflux esophagitis from December 2015 to December 2016 in our hospital were retrospectively analyzed, were randomly divided into two groups according to the time of admission.The control group (157 cases)was treated with rabeprazole, the observation group (158 cases) given rabeprazole combined with mosapride treatment.The clinical symptom scores before and after treatment, the effective rate of treatment, the quality of life before and one month after treatment and three months after treatment of two groups were compared.ResultsAfter treatment,the symptoms scores of the two groups were significantly lower than those before treatment, but the the scores in the observation group were significantly lower than those in the control group (P<0.05);The total effective rate was 96.2% in the observation group, which was significantly higher than that in the control group (79.0%), the difference between the two groups was statistically significant (P<0.05);The quality of life scores of the two groups were significantly higher than that before treatment, but the scores of one months after treatment and three months after treatment in observation group were significantly higher than those in control group (P<0.05).ConclusionReflux esophagitis with rabeprazole combined with mosapride treatment is significant,can better improve the clinical symptoms of patients, improve the treatment efficiency, and improve the quality of life of patients.

This study examined the effects of a recombinant adenovirus Ad-PTEN-EGFP on the proliferation of A549 cells, a human lung carcinoma cell line, in vitro and on the growth of the implanted tumors in the nude mice in vivo, explored the underlying mechanisms and evaluated the in vitro transfection efficiency of Ad-PTEN-EGFP into A549 cells. The expression of Ad-PTEN-EGFP in the A549 cells was determined. The proliferation and the apoptosis rates of the A549 cells with Ad-PTEN-EGFP transfection or not was detected by MTT and flow cytometry. Ad-PTEN-EGFP at different doses was injected intratumorally to the tumor-bearing mice induced by the A549 cells. Tumor sizes were measured on an alternate day. After all the mice were sacrificed, the implanted tumors were removed for routine histological examination, weight test, HE staining and immunohistochemical staining. The expressions of Bax, P16 and P53 in the tumor tissues and those of caspase-3, CD34 and VEGF in the mouse sera were detected. Tumor cell apoptosis was measured by TUNEL method. The results showed that the vitality of the A549 cells after transfection with Ad-PTEN-EGFP declined. The expression of green fluorescent protein was observed under fluorescent microscope. The transfection rate was in excess of 50%. The mRNA and protein expression of PTEN in the transfected cells was confirmed. The proliferation rate of the transfected cells was significantly decreased when compared with that of the non-transfected cells (P<0.05). The number of the apoptosis cells was increased in the transfected cells (P<0.05). The models of implanted tumors were successfully established by injection of the A549 cells in the flank of Balb/c nude mice. Administration of Ad-PTEN-EGFP to the tumor-bearing nude mice resulted in a suppression of tumor growth. There were statistically significant differences in the tumor weight and tumor volume between the Ad-PTEN-EGFP-treated group and the control groups (P<0.05). In contrast to those in the control groups, tumor tissues in the Ad-PTEN-EGFP-treated group were shown to have typical extensive vacuolar degeneration and massive hemorrhagic necrosis. Apoptotic bodies were also observed in the tumor cells. The expressions of Bax, caspase-3 and P16 were increased (P<0.05) while those of CD34, VEGF and P53 decreased (P<0.05) in the Ad-PTEN-EGFP-treated group. It is concluded that Ad-PTEN-EGFP could induce the apoptosis of the A549 cells and inhibit their proliferation. And it could also substantially suppress the tumor growth in the tumor-bearing nude mice and induce apoptosis of the tumor cells as well. These findings carry significant implications for adenovirus vector-based PTEN gene therapies for lung cancers.

Objective Analysis the bacteria distribution and drug resistance change in our hospital from 2011 to 2013 ,and pro‐vide evidence for clinical rational use of antimicrobial drugs .Methods Isolate all kinds of pathogens from outpatient ,inpatient from 2011 to 2013 in statistics and the analysis of drug resistance .Results The top four highest separation rates were Escherichia coli , Pseudomonas aeruginosa ,Acinetobacter baumannii and Staphylococcus aureus .Methicillin‐resistant staphylococcus aureus (MRSA) separation rate of 3 years were 52 .17% ,57 .9% ,55 .52% respectively .Conclusion Production extended spectrum β‐lactamases (ELBLs) of Escherichia coli has a tendency to increase;the third generation cephalosporins and aztreonam resistance rates also show an upward trend ,Carbapenems Enterobacteriaceae is still the most active drugs;Glycopeptides ,linezolid ,moxifloxacin ,and rif‐ampicin always maintain a high activity against MRSA ;the resistant rates of non‐fermenting bacteria is higher ,we need to concerned about the emergence of multi‐drug resistant .Recommend giving antibiotics under the guidance of susceptibility results .

Fascin,an actin cross-linking protein found in membrane ruffles,microspikes,and stress fibers,induces membrane protrusions and increase cell motility in various transformed cells.The expression of fascin in epithelial neoplasms has only been described recently,and fascin overexpression may play an important role in the progress of neoplasms.This article reviews the current situation and the up to date progress of study concerning fascin and epithelial neoplasms.