Objective To evaluate the value of serum tumor markers in diagnosis of over 80 years old patients with colorectal cancer.Methods Serum CA199,CA242 and CEA of 68 cases senile patients with colorectal cancer and 35 cases senile patients with benign large intestinal diseases were detected by ELISA.Results Serum levels of CA199,CA242 and CEA in patients with colorectal cancer were (37.95 ± 15.95 ) U/ml,( 50.08 ± 24.20 ) U/ml,( 22.34 ± 17.35 ) μg/L respectively and were significantly higher than that in patients with benign large intestinal diseases CA199 ( 10.73 ± 6.27 ) U/ml,CA242 ( 8.00 ± 3.52 ) U/ml,CEA(2.32 ± 1.01 )μg/L.In addition,the level of these three markers was increased with the progress of colorectal cancer and the sensitivity of diagnosis was related with the stages of colorectal cancer.Parallel combined testing methods increased the diagnostic sensitivity to 83.6％,and serial combined testing increased the diagnostic specificity to 88.7 ％.Conclusions Serum tumor markers showed certain values in the diagnosis of the senile patients with colorectal carcinoma.Furthermore,the combined determination of tumor markers is helpful for early diagnosis of colorectal cancer and increases the sensitivity and specificity in diagnosing of colorectal carcinoma.

OBJECTIVE:To observe the curative and preventive effects of reco mbinant basic fibroblast growth factor(rbFGF)on radio mucitis.METHODS:120patients with head and neck carcinoma were randomly divided into the therapy group and control group with60patients in each group,1week after radiotherapy till the end,the therapy group were given1ml of rbFGF by oral spraying5puff,3times daily after meal and the patients in the control group mouthwash with compound borax solution3times daily after meal.RESULTS:Radio mucitis in therapy group was less serious than that in the control group(P

Mitochondrial oxidative stress has been recognized as a preliminary and critical factor that aggravates the pathological cascade of Alzheimer's disease, which induces the production of β-amyloid protein, upregulates the expression of phosphorylated tau protein and triggers oxidative damage to lipids, proteins and mitochondrial deoxyribonucleic acid. Central neurons are more vulnerable to oxidative stress than non-neuronal cells due to their high oxygen demand, abundant unsaturated fatty acids and antioxidant enzymes deficiency. On this account, this review introduces the causes of mitochondrial oxidative stress, and analyzes the important role of mitochondrial oxidative stress in the pathogenesis of Alzheimer's disease. Meanwhile, the review focuses on the design and intervention strategies of drug delivery systems targeting mitochondrial oxidative stress in neurons, aiming to provide new ideas for the prevention and treatment of Alzheimer's disease.

Objective To evaluate the therapeutic effect and possible mechanism of intrapleural administra- tion of cisplatin bound to activated carbon particles for treating malignant pleura] effusions(MPE). Methods ①60 patients with MPE confirmed in the Third Hospital of Changzhou and the Second Hospital of Changzhou from 2004 to 2007 were randomly divided into treatment group (n=30)and control group(n=30). Chest catheters were inserted percutaneously into the pleural space to drain the effusions. Cisplatin mixed with activated carbon particles for the treatment group and only cisplatin for the control group were injected into pleural cavity. Whether above-mentioned treatment should be repeated was determined by ultrasonic B every week until up to four times. Curative effect and side-effect were compared between two groups a month later. ②20 cases, randomly selected from both groups respectively, underwent whole body and chest SPECT scan to image lymphatic system by means of 99Tcm-DX after effusions drainage but before intrapleural injection of drug. Cases whose imaging graphics were abnormal would undergo the above SPECT again 2 weeks after intrapleural injection of drug so as to find changes in imaging graphics. Results ① The overall response rate was significantly higher in treatment group than in control group(100% (30/30) vs 66.7% (20/30), χ2=12.00, P<0.01)and that intrapleural injection was needed only once in most cases. Gastrointestinal upset and leucoponia were less and milder in treatment group than in control group (16.7% (5/30) vs 30.0% (9/30) and 6.7% (2/30) vs 20.0% (6/30) respectively),but there was no statistical difference between the two groups(χ2=1.49 and 1.30,P>0.05). ②The rate of improvement in lymphatic imaging was significantly higher in treatment group than in control group (78.6% (11/14) vs 37.5% (6/16),P<0.05). Conclusion The treatment of malignant pleural effusions with intrapleural administration of cisplatin bound to activated carbon particles is extremely effective and safe, and can improve lymphatic drainage as well.

Objective To study the value of tumor markers of CA153,CEA and CA125 in nipple discharge of breast intraductal papillary leision on detection of breast carcinoma.Methods 154 cases of breast intraductal papillary leision with nipple discharge were studied.Among them there were 58 cases with breast intraductal papillary carcinoma and 96 cases with intraductal papilloma.The nipple discharged and serum from the 154 cases were collected and CA153,CEA and CA125 levels were measured both in nipple discharge and serum with electrochemiluminescence method,and were compared with the results of ER,PR,HIF-1α and Ki-67 detected by SP method in breast tissue.Results The CA153,CEA and CA125 levels of nipple discharge in intraductal papillary carcinoma [(130.1 1±29.62) U/ml,(89.23±28.94) ng/ml,(41.29±16.61) U/ml]were significantly higher than those of the contrast groups,and had a positive correlation with the Ki-67,HIF-1αand lymphnode metastas (P ＜ 0.05),and negative correlation with the level of ER,PR and Her-2 (P ＜ 0.05).The positive rate of CA153,CEA or CA125 in nipple discharge (62.07 ％,46.55 ％,55.17 ％) were significantly higher than that in serum (P ＜ 0.05).The sensitivity of the combined detection of the three tumor markes both in discharge and serum was 96.55 ％,and the negative predictive value was 97.30 ％,which were significantly higher than that in other detection (P ＜ 0.05).Conclusions The positive rate of CA153,CEA and CA125 in nipple discharge were significantly higher than that in serum.The dynamic combined detection of CA153,CEA and CA125 both in nipple discharge and in serum could increase the diagnosis rate of breast intraductal papillary carcinoma.

Objective To evaluate the genotoxicity of europium nitrate to the Vicia faba L root-tip cells. Methods The primary roots of Vicia faba L were cultured for 6 hours in the solution of Eu series(64,32,16,8,4,2,1 ?g/ml) at 25℃.And then the roots were repair-cultured in double distilled water for 24 h. The root tips of Vicia faba L were dissected and fixed,stained and the micronucleus rate,mitotic index and the rate of chromosomal aberrations were determined. Results From 1 ?g/ml to 32 ?g/ml,the rate of micronucleus increased with the increasing of the europium nitrate dosage(D),presented a dosage-effect relationship(r=0.949). From 2 ?g/ml to 64 ?g/ml,mitotic index decreased with the increasing of the europium nitrate dosage,presented a dosage-effect relationship(r=-0.852). From 1 ?g/ml to 64 ?g/ml,chromosome fragments(CF) and frequency of chromosomal aberrations(FCA) increased with the increasing of the europium nitrate dosage with a dosage-effect relationship significantly(rD-CF=0.915,rD-FCA=0.872). Conclusion Europium nitrate has the certain genetic toxicity to the Vicia faba L root-tip cells. The chromosome fragments may be one of the reasons to produce micronucleus.

The special environmental features of high altitude, such as hypobaric hypoxia, low temperature, arid, high solar radiation, variable climate and geochemical anomaly, cause great effects on human physiology and health. It will provide valuable references and new ideas to study drug's metabolism in special environment of high altitude hypoxia, and give the guidance to clinical reasonable medication, avoiding adverse reactions and personalized medicine in plateau areas. This article reviewed the effect of high altitude hypoxia on drug metabolism, elaborated metabolic characteristics of some drugs and the activity and expression of drug metabolism enzymes under hypoxia environment at high altitude, and discussed related mechanism.
Altitude ; Climate ; Cold Temperature ; Humans ; Hypoxia ; Pharmaceutical Preparations ; metabolism

Objective To investigate the effect of tacrolimus on the expression of nuclear factor-κB (NF-κB) in HaCaT cells stimulated by tumor necrosis factor-α(TNF-α),and on the expression of glucocorticoid receptor (GR)α and β in untreated HaCaT cells in vitro.Methods Cultured Ha CaT cells were treated with TNF-α(10μg/L) only,combination of TNF-α(10μg/L) and various concentrations (10-8mol/L, 10-7mol/L,10-6moL/L) of tacrolimus or tacrolimus of different concentrations only.After additional 12-,24-, 36- or 48-hour cnlture, Westem blot and immunofluorescenee-confocal laser scanning microscopy were used to detect the expressions of NF-κB,GRα and GRβ in HaCaT cells.Those untreated HaCaT cells served as the control.Results The relative protein expression level of NF-κB was increased in HaCaT cells after treatment with TNF-α for 24 and 48 hours zompared with untreated ceils (0.73±0.0316 and 0.8925±0.0171 vs 0.4988±0.03506,both P<0.05);however,the increase in NF-κB expression was inhibited by the combination treatment with tacrolimus,and the relative expression level of NF-κB protein was 0.6825±0.0263.0.6200±0.0163 and 0.5575±0.0299 in HaCaT cells treated with TNF-α plus tacrolimus of 10-8mol/L 10-7mol/L and 10-6mol/L,respectively;the difference was significant etween TNF-α-treated cells and those dealt with the combination of NF-α and tacrolimus of 10-7 or 10-6 mol/L (both P<0.05).No significant difference was observed in the expression of NF-κB by HaCaT cells between different time oints treated with tacrolimus of 10-8,10-7 or 10-6 mol/L.Also,there was no zignificant difference in the expression of GRα or GRβ between untreated HaCaT cells and those treated with tacrolimus of 10-8, 10-7 or 10-6 mol/L at any time point.Conclusions Tacrolimus ould inhibit the expression of NF-κB by TNF-α-stimulated HaCaT cells,but does not affect the expression of GRα or GRβ,in untreated HaCaT cells.

Objective Analysis of clinical distribution and susceptibility to antimicrobial agents in producing extended spectrumβ-lactamases (ESBLs)of Escherichia coli(ECO)and Klebsiella pneumoniae (KPN).Methods The samples from 2013 April to 2014 December in Changji Prefecture People′s Hospital were cultured and identified,drug sensitivity test did by VITEK2compat au-tomatic bacteria analysis,phenotypic confirmatory test was did on producing ESBLs ECO and KPN,and the clinical distribution and drug resistance was analyzed.Results 1 576 strains of ECO and KPN isolated 306 strains of producing ESBLs strains and 343 strains of multi drug resistant bacteria,the isolation rates were 1 9.4% and 21.8% respectively.In 576 strains of ECO,there were 1 77 strains of producing ESBLs strains and 202 strains of multi drug resistant bacteria were isolated,the separation rates were 30.7% and 35.1% respectively,generic drug resistant strains were not found,and those producing ESBLs strains were isolated mainly from the urine specimens (39.5%),and mainly from the ICU (37.3%)and (29.9%)the surgical ward.There were 129 strains of producing ESBLs strains,141 strains of multi drug resistant bacteria and 4 generic drug resistant strains were isolated from 1 000 strains of KPN,the separation rates were 12.9%,14.1% and 0.4% respectively,and those producing ESBLs strains were isolated mainly from sputum specimens (83.7%),and mainly from the ICU (42.6%)and(29.5%)in the department of inter-nal medicine.Isolation of producing ESBLs ECO and multi drug resistant bacteria were higher than KPN,the difference were statis-tically significant (P <0.05).The sensitivities of ECO and KPN ESBLs strains to carbapenems imipenem andaminoglycosides ami-kacin and compound preparation piperacillin/tazobactam were good,the other antimicrobial drug resistance rates were more than 50.0%.Conclusion ESBLs ECO mainly causes urinary tract infections,KPN mainly causes respiratory tract infection.Detection rates of producing ESBLs ECO and KPN were high,and the multi drug resistance is serious,should strengthen the monitoring of drug resistance on producing ESBLs strains,and according to the results of drug susceptibility,standardize the rational use of anti-microbial agents,and to strengthen the management of antimicrobial agents,delay the occurrence of bacterial resistance.

AIM To study the flavonoids from Morus nigra L.leaves.METHODS The n-butanol fraction of 80％ ethanol extract from M.nigra leaves was isolated and purified by macroporous adsorption resin and preparative HPLC column,then the structures of obtained compounds were identified by spectral data.RESULTS Eleven compounds were isolated and identified as quercetin (1),icariside Ⅱ (2),rhodionin (3),rhodiosin (4),quercetin-3-O-β-(2-O-acetyl) galactopyranoside-7-O-α-arabia pyran glucosides (5),salidroside (6),kaempferol-3-O-β-(2-O-α-L-rhamnopyranosyl)-glucuronide (7),quercetin-3-O-α-rhamnopyranosyl-(1 →2)-β-glucopyranosiduronide (8),rhamnetin-3-O-galactoside (9),quercetin-3'-O-β-glucoside (10),quercetin-3-O-β-D-glucopyranoside (11).CONCLUSION Compounds 1-10 are isolated from this plant for the first time.