OBJECTIVE:To analyze clinical influencing factors and monitoring results of serum concentration of digoxin (SDC) to provide reference for rational use of drug. METHODS: 137 medical records were collected and analyzed statistically with multiple regression analysis in order to study the relationship of SDC with influencing factors. RESULTS: SDC was significantly affected by dosage, height, body weight, free fat body weight, body surface area, creatinining clearance rate, serum creatinine (P

Objective To investigate the CT features of desmoplastic small round cell tumor (DSRCT)occurred in abdomen and pelvis.Methods The CT images of 4 patients with pathologically proven DSRCT occurred in abdomen and pelvis were retrospectively analyzed.Results The CT features common to the 4 DSRCT in abdomen and pelvis were as follows:the lobulated soft-tissue masses were very large;the masses often involved omental and serosal surfaces of abdomen and pelvis organ and had close relationship to the surrounding organs;the originating site of the tumors were not apparent;intratumoral hemorrhage or necrosis were often present;and contrast-enhanced CT scan showed mild to medium heterogeneous enhancement.Conclusion The CT features of DSRCT in abdomen and pelvis are rather specific and can provide helpful information to the clinical diagnosis.

Objective To compare the differences of salivary alpha(α) amylase (sAA) activity and its activity ratio from the sali‐va before and after citric acid stimulation and approach the correlations among sAA activity determined by the methods of iodine‐starch ,Bernfeld and EPS‐G7 velocity respectively .Methods Ten saliva samples were collected from five healthy volunteers before and after citric acid stimulation .Their activities were determined three times by the three methods ,and the variation coefficient (CV) of sAA activity and activity ratio were calculated .Moreover ,correlation among sAA activities determined by the three meth‐ods were analyzed .Results The significant differences (P< 0 .05) were found in sAA activity and total CV from three determined methods ,and sAA activity and total CV by the method of EPS‐G7 velocity were minimum .There were no significant differences (P> 0 .05) in sAA activities ratio and its CV ;Significant correlation was found between sAA activity determined by random two of three methods(P < 0 .05) ,and their correlation coefficients were above 0 .96 .Conclusion The sAA activity data determined by three methods could be transformed each other by regression equation ,and determined precision by the method of EPS‐G7 velocity is highest ,and data processing method of sAA activity ratio could decrease differences among CV from three methods .

Objective To investigate the expression of S-phase kinase associated protein 2 (Skp2) in rats with acute liver failure (ALF) and its significance. Methods There were 256 male SD rats used in this study, among which 240 were injected with D-galactosamine (D-GaIN) to set up ALF model. The rats were divided into 3 groups: ALF model group, free hepatocellular transplantation group, microencapsulated hepatocyte transplantation group, which were intraperitoneally injected with 2 mL of RPMI 1640 culture medium, free hepatocellular suspension and microencapsulated hepatocyte suspension, respectively. The other 6 rats were in control group and the rest 10 rats were used for hepatocyte isolation. Expressions of Skp2 protein in hepatocytes of rats at different time points were detected by immunohistochemical technique. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBil) were detected by automatic biochemistry analyzer. The survival rate in each group was observed. Comparisons among groups were done using one-factor analysis of variance. Results Levels of ALT, AST and TBil decreased more significantly by intraperitoneal transplantation of microencapsulated hepatocytes than those by intraperitoneal transplantation of free hepatocytes (P<0. 05). Skp2 labeling indices after 36 h of injection in ALF model group, free hepatocellular transplantation group and microencapsulated hepatocyte transolantation grouo were (28. 2±6.1) %, (41.4± 10. 5) % and (68. 0±10.8) %, respectively (F=29. 08 , P<0. 05). There were 4, 6 and 11 out of 15 rats survived in the 3 groups, respectively. Conclusion The dynamic observation of Skp2 expression could be used to judge the regeneration of hepatocytes.

Objective To study the potential of bFGF to promote human bone marrow derived MSCs to differentiate into cardiomyogenic cells, and its effect on proliferation of MSCs. Methods MSCs isolated from adult human bone marrow were cultured in four different systems. Group A: medium with 5-aza. Group B: medium with bFGF. Group C: medium with 5-aza+bFGF. Control group: medium alone. The morphological changes of MSCs were observed. Then immunocytochemistry staining against ?-actin,cTnT,and Connixin43 was performed. The expression of Nkx2.5, GATA-4 and cTnT was detected by semi-quantitative RT-PCR. The proliferation of MSCs in different groups was measured by MTT. Results The MSCs in both group A and C partially differentiated into myogenic cells and expressed proteins of ?-actin,cTnT,and connixin43. In group A and group C, the mRNA level of Nkx2.5,GATA-4 and cTnT was higher than that of control group. In group B, mRNA level of Nkx2.5 and GATA-4 was higher than that of control group. As compared with the control group, cell proliferation was faster in group B than in group A and C. And the proliferation was faster in group C than in group A. Conclusion bFGF can promote the proliferation of MSCs significantly. When combining with 5-aza, bFGF can promote MSCs to differentiate into cardiomyogenic cells more effectively.

Objective To observe of therapeutic effect of bamboo shoots extract compound on the treatment of hemorrhagic anemia.Method The glucose,total protein,amino acids,trace and macro elements in bamboo shoots extract were determined.Through the performance of angular vein bloodletting,a hemorrhagic anemia rat model was set up.Two therapy groups received the intragastric administration of compound Ⅰ(0.15% iron porphyrin and 10% honey) and compound Ⅱ(70% bamboo shoots extract,0.15% iron porphyrin and 10% honey),respectively.And the control group only received the de-ionized water of the same dose.The blood samples,collected via tail vein in 0,18,42,67 and 90h after the administration,were detected for hemoglobin,red blood cell and hematocrit.And the acute toxicity test was also conducted.Result Compound Ⅱ,which contained the Bamboo shoots extract that was rich in glucose,amino acids,amino,and trace and macro elements,had a better efficacy in treating hemorrhagic anemia of rats than the Compound Ⅰ.And the result of acute toxicity was normal. Conclusion Bamboo shoots extract compound could promote the recovery of hemorrhagic anemia.

Objective To evaluate the efficacy of endoscopic techniques for corrections of facial contour deformities.MethodsA total of 58 patients(48 women and 10 men)with square face(38),prominent zygoma(12),or crooked noses(8)were treated by endoscopic-assisted technique in our hospital.Results The operation time ranged from 40 to 86 min(mean,65 min),and intraoperative blood loss was 60-120 ml(mean,78 ml).The patients were discharged from the hospital in 3 to 7 days(mean 4)after the surgery,and was followed up for 3 to 24 months.During the period,no one had unexpected fracture,injuries to the mental and facial nerves and vessels,or infection.All the patients were satisfied with the surgical outcomes.ConclusionsEndoscopic-assisted correction of facial contour deformities is feasible,safe,and effective.

1:1,and fat emulsion's energy delivery exceeding 50% of total non -protein calories.CONCLUSION: It' s high time to set up a parenteral nutrition support team, enabling clinical pharmacists to assist physicians with the regulation of PN support in parenteral nutrition dispensing.

Objective To observe the spatial and temporal expression patterns of FGFR3 gene and analyze the potential roles of FGFR3 during fracture healing and explore the technique of in situ hybridization of paraffin sections of bone tissue. Methods The fracture model of mouse tibia was established by the standard methods. The expression of FGFR3 mRNA was studied by in situ hybridization during the various stages of bone repair. Results There was faint FGFR3 positive-signal in the cytoplasm of prehypertrophic chondrocytes in soft callus on day 5. FGFR3 mRNA was strongly expressed in prehypertrophic chondrocytes and hypertrophic chondrocytes in soft callus on day 7, but disappeared on day 14, 28 because chondrocytes was replaced by osteoblasts. Conclusion FGFR3 mRNA was strongly expressed in prehypertrophic chondrocytes and hypertrophic chondrocytes in the fracture callus, which shows that FGFR3, a gene that regulates the proliferation of chondrocytes during bone development, may participate in the regulation of differentiation of chondrocytes during fracture healing.

The respiratory control ratio (RCR), the rate of O2 consumption in state 3 and the rate of ATP formation of liver mitochondria succinate respiratory chain were increased 30 min after Sprague-Dawley rats were injected with epinephnne or norepinephrine. The rate of O2 consumption in state 4 was increased or slightly increased. When rats were injected with adrenergic blocking agents, timolol and phenoxybenzamine, 1 h before burn, the activities of succinate respiratory chain were partly inhibited 30 min post-burn. All the results suggest that catecholamines play a main role in the increase of oxidative phosphorylation coupling in the early phase of burn injury.