Objective To investigate the clinical effect of vertical incision for breast surgery.Methods According to Lejour method,the first breast shape on the patients who needed operation was designed,and make a mark line corresponding to the breast,and then removal of excess tissue during the operation,operation process should pay attention to the protection of important part of breast of vessels and nerves,to prevent the impact of breast lactation function.Results The operation in 48 patients with breast because of too much too small in patients with 2 cases,1 cases of patients areola was too large,the patients were followed up survey,all the patients were satisfied with the operation of the breast shape,1 cases of operation the scar not satisfied,then the operation scar resection of operation after further results were satisfied,the total satisfaction rate was 98%.Conclusion Breast reconstruction process using vertical incision method for patients with relatively small,less bleeding,almost did not affect the normal function of patients with breast,breast shape is preferred for patients with operation scheme.

Flowering, the floral transition from vegetative growth to reproductive growth, is induced by diverse endogenous and exogenous cues, such as photoperiod, temperature, hormones and age. Precise flowering time is critical to plant growth and evolution of species. The numerous renewal molecular and genetic results have revealed five flowering time pathways, including classical photoperiod pathway, vernalization pathway, autonomous pathway, gibberellins (GA) pathway and newly identified age pathway. These pathways take on relatively independent role, and involve extensive crosstalks and feedback loops. This review describes the complicated regulatory network of this floral transition to understand the molecular mechanism of flowering and provide references for further research in more plants.
Arabidopsis ; physiology ; Flowers ; physiology ; Gene Expression Regulation, Plant ; Gene Regulatory Networks

Objective To establish a rapid, sensitive, and specific quantitative method to detect hepatitis C virus. Methods A primer set targeting HCV 5'UTR was designed. The isothermal amplification was performed by the Bst DNA polymerase and AMV reverse transcriptase, under the temperature of 60℃ for 60 min. The signal was monitored by SYBR Green Ⅰ. Results One hundred and twenty positive serum samples, confirmed by the real-time PCR. All were detected by the isothermal amplification, while 110 healthy subjects' samples were negative by the both methods. The lower detect limit was determined to 10 IU/ml HCV-RNA, by the assay on serial dilutions of the quality control standards obtained from clinical investigation center of MOH. Conclusion A real time reverse loop-mediated isothermal amplification method was developed to detect HCV, with the characteristic of rapidity, high sensitivity and specificity.

Vaspin,a serine protease inhibitor derived from visceral adipose tissue,has been identified as an adipokine with insulin-sensitizing effects in a rat model of type 2 diabetes.Recent studies showed that the administration of recombinant human vaspin significantly improved insulin sensitivity and glucose tolerance,and reversed the expression of genes that may promote insulin resistance in diet-induced obese mice.However,the regulation of serum vaspin concentration in individuals with insulin resistance,and the association of vaspin with obesity,type 2 diabetes,and insulin resistance related disorders remain unclear.

Tumor multidrug resistance (MDR) is the leading cause of clinical chemotherapy failure and death.Researches show that the occurrence of MDR is related to P-glycoprotein,multidrug resistance-associated proteins,breast cancer resistance-associated proteins,lung resistance-related proteins and other factors.Now the reversal strategies of MDR include chemical drugs reversal,gene reversal,immune reversal,traditional Chinese medicine reversal and drug-loaded nano-system reversal,which make some progress and help to improve the effect of cancer chemotherapy.

Objective To investigate the antibiotic-resistant gene mecA and the prevalence of Panton-Valentine leukocidin (PVL) gene, toxic shock syndrome toxin (TSST-Ⅰ) gene in S. aureus. Methods A total of 74 S. aureus were collected from clinical specimens. The mecA, PVL and TSST-Ⅰ genes were detected by PCR.Results PVL gene was identified in 22 S. aureus isolates. The prevalence of PVL was 29.7% in S. aureus, 36.6% in MRSA and 21.2% in MSSA. The difference was not statistically significant (P>0.05). The prevalence of MRSA was 55.4% in 74 S. aureus. The prevalence of TSST-Ⅰ gene was 6.8%. TSST-Ⅰ gene was not detected in MSSA.Conclusions MRSA strains show highly resistant to antibiotics. PVL- and TSST-Ⅰ-positive S. aureus are more pathogenic.

Objective:To investigate the regulatory effect of dissociative intracytoplasmic domain of leukemia inhibitory factor receptor ? (LIFR?) subunit on the growth of HL-60 cells and the related signal transduction mechanism. Methods: The constructed recombinant plasmids pcDNA3.0-190CT2+3 and cDNA3.0-190CT3 were identified and transfected into HL-60 cells with liposome FuGene-6. Then the cells were cultured in a medium containing G-418 and the positive clones were selected. Immunohistochemical method and Western blotting were used to detect the expression of the target protein in HL-60 cells and the growth curve of the cells was plotted. The expression of proliferating cell nuclear antigen (PCNA) and the phosphorylation levels of signal transducer and activator of transcription 3 (Stat3) were assayed by Western blotting. Results: Western blotting detected the band of target protein and the cell line stably expressing the target protein was obtained. Compared with that of the wild type group, the cell sizes in 190CT2+3 and 190CT3 group were enlarged. The ratio of leaf cell number to the total cell number increased and the cell proliferation was slowed down. We also found that the expression of PCNA was decreased and the phosphorylation level of STAT3 was increased. Conclusion: The dissociative intracytoplasmic domain of LIFR? subunit can accelerate the differentiation of HL-60 cells, inhibit the proliferation of HL-60 cells, and activate signal molecule STAT3.

Objective: Exploring the epidemiological state of pervasive developmental disorder in 2-6 year-old children in Beijing. Methods: A total of 21866 children aged 2-6 year-old were recruited from permanent residents of Beijing by 2-phase cluster sampling.The Clancy Autism Behavior Scale was used for screening.The Childhood Autism Rating Scale and DSM-Ⅳ were used for diagnosis.Results: 16 children were diagnosed as pervasive developmental disorder (14 with autistic disorder, 1 with atypical autistic disorder, 1 with Rett syndrome).The false negative rate was 0.80 ‰.The prevalence of pervasive developmental disorder was 0.73 ‰, the adjusted prevalence was 1.53 ‰,the average annual detective rate was 0.11‰.The prevalence rate had no difference in distribution between urban-rural, among different age groups, sex and nations(?2=0.11~1.85,P=0.739-0.173).The low family income was related to high prevalence significantly ( tendency ?2=4.70,P=0.030 ).25% children with pervasive developmental disorder had not been identified by parents or had not been to clinics.80% children with pervasive developmental disorder had not get intervention.All children had demand to get intervention. Conclusions: The prevalence of pervasive developmental disorder in 2-6 year-old children in Beijing was not low.Low family income is the risk factor.The rate of intervention was low.The government should pay close attention to the children with pervasive developmental disorder and their needs.