1.Traumatic abdominal hernia
Shubao YANG ; Yang ZHAO ; Qiaofeng CHENG
Chinese Journal of Primary Medicine and Pharmacy 2006;0(09):-
Objective To increase the knowledge of traumatic abdominal symptom.Methods The clinical datas of 3 cases undergoing traumatic abdominal hernia were retrospectirely reviewed including.Male 2 cases and female 1 case.The average age was 34 years old.3 cases were impacted by carpentry car or with a intumescent tumor painly.Results The 3 patients were operated with return intestingal canal hernioplasty,the splanchnic organ in abdomiral cavity weren't injured.Conclusion The illness is caused by great straight passiration injmy and caused abdominal inferior mriscles contract and dilacerate.Because this illness usually has the atrium injured,active opertion not only can avoid the abdominal lesion,but also can do the return intestinal canal herniolasty.
3.Diagnosis and management of complications in nephrotic syndrome
Liping ZHAO ; Lingyun YANG ; Yun CHENG
Chinese Journal of Applied Clinical Pediatrics 2016;31(5):333-336
Nephrotic syndrome(NS)is the most frequent cause of glomerular disease in children,it can deve-lop many complications,including infection,thrombosis and acute kidney injury,while acute adrenocortical suppression is a complication of some drugs administration. This review focuses on the pathophysiology and management of common complications in patients with NS.
4.Changes in energy metabolism and serum enzyme biomarker under static load in rabbits.
Can WANG ; Su ZHAO ; Cheng-Jun YANG
Chinese Journal of Applied Physiology 2008;24(2):150-165
Animals
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Creatine Kinase
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blood
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Energy Metabolism
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Lactate Dehydrogenases
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blood
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Male
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Pressure
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adverse effects
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Rabbits
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Serum
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enzymology
5.Histological observation on the urinary organs in normal Meriones Unguieulataus
Wenjie ZHAO ; Zhiqi SONG ; Guangyu CHENG ; Deming ZHAO ; Lifeng YANG
Chinese Journal of Comparative Medicine 2014;(12):24-26
Objective Mongolian gerbil can make themself urine concentration for saving water and adapt to the harsh desert environment, due to their very unique moisture control system in the body.Methods Mongolian gerbil is resistant to drought on account of their special kidney. Histology of the kidney, ureter and bladder in Meriones Unguiculataus were observed by light microscopy using HE staining.Results The results showed that compared with rats and mice, the Mongolian gerbils have more developed distal tubules, and well developed inner renal medulla.Conclusions We hope that the findings of this study enrich our understanding of the histology of urinary system in Mongolian gerbils and provide support for the laboratory animalization of this animal.
6.Construction of traditional Chinese medicine resources information spatial database.
Yu-yang ZHAO ; Cheng-zhong SUN ; Ze-dong YANG
China Journal of Chinese Materia Medica 2015;40(6):1219-1222
The informatization of traditional Chinese medicine resources is the basis of modern medicine. With a spatial attribute traditional Chinese medicine resources could be carried out for in-depth spatial analysis, data mining and traditional Chinese medicine resources regional industrial layout. In this paper, we took the data of Glycyrrhiza uralensis in the third national Chinese medicine resources survey as the experimental data, described the principles and structure of traditional Chinese medicine resources spatial information database. We also described the establishment of analysis model with the help of this spatial database.
Data Mining
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Databases, Factual
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Medicine, Chinese Traditional
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Plants, Medicinal
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chemistry
7.Rifaximin versus Ciprofloxacin for Treatment of Acute Infectious Diarrhea
Kabing ZHAO ; Liufang CHENG ; Lihua YANG ; Rongbin GUO ; Yunsheng YANG
Chinese Journal of Nosocomiology 2006;0(12):-
0.05).The incidence of adverse events was low and similar in each group. CONCLUSIONS Rifaximin is a safe and effective alternative to ciprofloxacin in the treatment of acute infectious diarrhea in adults.
8.Construction and identification of a cDNA library of human normal kidney tissues
Yu CHENG ; Xu LI ; Wei CHEN ; Le ZHAO ; Yucong YANG
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To construct a full-length cDNA library of human normal kidney tissues and identify the quality of the library. Methods By using the template-switching mechanism at 5′end of mRNA technique to construct the library, a powerscript reverse transcriptase was used to transcribe, and a 5′-oligo fragment as an extended template was added to 5′ end of mRNA to enrich full-length cDNAs. After amplification, the ds cDNAs digested by sfi I and size-fractionated by columns were recombined into ?TripIEx 2 vectors. After package, the recombinant vectors were titered and the recombinant rate (blue/white) was determined,then the library was amplified. We identified the library using PCR reaction to determine the size of the inserts. Results The titer of cDNA library was 2.6?10 6pfu?mL -1, the rate of recombinant was above 95%, and the titer of amplified library was 9?10 11pfu?mL -1. The insert size ranged from 0.7 to 2 kb. Conclusion The cDNA library of human normal kidne we constructed is a highly efficient one and can be used for screening by probe and antibody to find the genes related to kidney diseases.
9.Effect of Busui Jiannao Prescription on ethology, GSH content and GSH-PX activity in brain of PD model rats
Li MA ; Weiping CHENG ; Jinuo ZHAO ; Yang ZHANG
International Journal of Traditional Chinese Medicine 2010;32(2):101-102
Objective To study the effect of Busui Jiannao Prescription on ethology, GSH content and GSH-PX activity in brain of PD model rats. Methods Forty-five the aging pure C57BL rats were divided into a negative injury group, a model group, and a Chinese medicine group. PD animal model were established by injection with MPTP and were treated with Busui Jiannao Prescription by intragastric administration. Score changes of PD model rats in the test of pole-climbing and suspending were observed. GSH content and GSH-PX activity were further measured. Results After treating with Busui Jiannao Prescription, the test scores in pole-climbing and suspending were improved, GSH content and GSH-PX activity in substantia nigra were increased. Conclusion Busui Jiannao Prescription can regulate the behavior coordination and repair the damage of dopaminergic neurons in the substantia nigra of PD model rats.
10.Effect of heat treatment on the viability of cultured normal human melanocytes
Jianrong NIU ; Qingqi YANG ; Rusong MENG ; Yu CHENG ; Guang ZHAO
Chinese Journal of Dermatology 2011;44(2):114-116
Objective To investigate the effect of heat treatment on the proliferation of, melanin synthesis and tyrosinase activity in cultured normal human melanocytes. Methods Normal human foreskin tissue was obtained by sterile circumcision and melanocytes were harvested by using methods for epidermal cell culture. Basic fibroblast growth factor (bFGF) was utilized as the primary mitogen to establish the culture system of normal human epidermal melanocytes. Masson-Fontana staining was proformed to identify melanocytes.Third-passage melanocytes were treated with hyperthermia at various temperatures (39 ℃, 41 ℃, 42 ℃, 43 ℃ and 45℃) for 1 hour a day for consecutive 3 days followed by the measurement of cell viability with MTT assay. The hyperthermia at optimized temperature was used to treat fourth-passage melanocytes for 1 hour a day for consecutive 3 days; subsequently, the tyrosinase activity were detected with L-Dopa as the substrate, and melanin content was determined in heat-treated and untreated (control) melanocytes. Results The hyperthermia at 42 ℃ exhibited the strongest promotive effect on the proliferation of melanocytes among these 5 hyperthermia conditions. After treatment with hyperthermia at 42 ℃ for 1 hour a day for consecutive 3 days, melanocytes showed an increment in tyrosinase activity by 36.4% and melanin synthesis by 78% compared with the untreated melanocytes (both P<0.05). Conclusions Heat treatment can enhance the proliferation of cultured human melanocytes, promote their melanin synthesis and tyrosinase activity.