Objective To examine the in vivo visual imaging of buccal carcinoma with the nearinfrared fluorescent quantum dots. Methods The U14 cells were labeled by endocytosis with QD800 (U14/QD800) which was linked with cell-penetrating peptide. Different number of U14/QD800 was injected under the buccal mucosa of nude mice and Kunming mice separately and imaged at different time to detect the in vivo sensitivity and dynamic imaging of U14/QD800. Results The minimum number of U14/QD800 cells which could be detected by in vivo imaging system was 1 × 104 in nude mice's cheek and 1 × 105 in Kunming mice's. The time for visual imaging of 1 × 104, 1 × 105 and 1 × 106 U14/QD800 cells in nude mice was 3, 7 and 16 d separately, and 3 and 10 d separately in Kunming mice. Conclusions Due to its strong tissue penetration, near-infrared fluorescent quantum dots have great prospects in cancer early diagnosis, visual observation and individual treatment.

Objective To evaluate the effectiveness of absolute alcohol in hemostasis in surgical resection of giant meningiomas with rich blood supply. Methods Twelve patients with giant meningiomas with rich blood supply were injected with absolute alcohol (8.5-27 mL, mean 11.2 mL) in the operations, and the tumors were resected under microscope. Results The tissues in the injection areas turned white and the tumors became hardened after the injection. The bleeding of the tumor surface and section was obviously decreased or even stopped. The quantities of bleeding were 48-154 mL in the operations, with an average of 67 mL. Total tumor resection was achieved in 11 patients (91.6%) and partial resection in 1 patient. No complications associated with the surgery were found in these cases. Conclusion Injection of absolute alcohol for hemostasis in surgical removal of giant meningiomas with rich blood supply is convenient and effective and can be widely applied in clinical practice.

Objective To explore the effect of HeLa cells infected with Coxsackie virus B3 (CVB3) on the changes of mTOR signal pathway under different nutritional conditions.Methods The HeLa cells were cultured under conventional culture and serum starvation culture.(1) For the conventional method,the medium with 10 g/L fetal bovine serum was added for 24 h after the Hela cells were fused into 40％ to 50％,and the medium was changed on the next day,then the virus group was infected with CVB3 of 50％ tissue culture infective dose (TCID50).However,the control group was cultured by 2 g/L fetal bovine serum.(2) For the serum starvation method,HeLa cells were cultured with the medium without fetal bovine serum for 24 h.Then the virus group was infected with CVB3 of TCID50.The cells in control group were cultured by 2 g/L fetal bovine serum.Cell morphology changes were observed by inverted microscope,and the expressions of the mTOR,p70S6K mRNA were detected with Real-time PCR at 3 h,6 h,9 h,12 h,24 h respectively in both conventional culture and serum starvation groups.Results The expressions of mTOR and p70S6K mRNA were lower in the virus group than those in control group at 12 h and the 24 h (all P ＜0.05) in the conventional culture group.And the expressions of mTOR and p70S6K mRNA in the virus group were lower than those in the control group at every time points (all P ＜ 0.05) in serum starvation group.The expressions of mTOR and p70S6K mRNA in group with serum starvation virus and the control groups were higher than those in conventional culture group in all time points,but only the expressions of mTOR mRNA were significantly different between the 2 groups (all P ＜0.05),however,the expressions of p70S6K mRNA had no significant difference between the 2 groups (all P ＞ 0.05).Conclusion CVB3 may be able to down-regulate the expressions of mTOR and p70S6K mRNA.

Objective Dyslipidemia and chronic inflammation are risk factors of cardiac fibrosis.This study was aimed to investigate their possible synergetic effects and underlying mechanisms on progression of cardiac fibrosis in apolipoprotein E knockout (ApoE-/-) mice.Methods Twenty-four ApoE-/-mice were divided into normal chow diet (control),high fat diet (HFD group),and HFD plus subcutaneously injection of 10％ casein (inflammation group) for 8 weeks.Lipid profile and serum amyloid A (SAA) were examined by clinical biochemical assays and Enzyme-Linked Immunosorbent Assay,respectively.Hematoxylin-eosin staining (HE) and Masson staining were used to evaluate the myocardial accumulation of lipid and collagen.Collagen Ⅰ protein expression was detected by immunohistochemical staining.Endothelial-to-mesenchymal transition related protein expressions were determined by Western blot.Results Serum SAA level was significantly higher in inflammation group [(127.42 ± 26.99) ng/ml] than in control [(15.40 ± 7.62) ng/ml] and HFD [(8.17 ± 0.72) ng/ml] group (all P ＜ 0.01).However serum levels of triglyceride,total cholesterol,and low density lipoprotein (LDL) cholesterol were significantly higher in HFD group than in inflammation and control groups[TG (7.53 ± 2.05) mmol/L vs.(3.43 ± 0.79) mmol/L ; TC (27.80 ± 3.99) mmoL/L vs.(14.94 ± 1.92) mmol/L ; LDL-C (11.56 ±2.56) mmol/L vs.(9.46 ± 1.31) mmol/L,all P ＜ 0.05).Foam cell formation in cardiac vessels.myocardial collagen deposit,protein expressions of collagen Ⅰ,CD31,and alpha-smooth muscle actin (α-SMA) were all significantly higher in inflammation group than in HFD group (all P ＜ 0.05) suggesting that inflammation contributes to the phenotype endothelial-to-mesenchymal transition in heart.Conclusion Inflammation exacerbates dyslipidemia mediated cardiac fibrosis in ApoE-/-mice partly through enhancing myocardial endothelial-to-mesenchymal transition.

Objective To evaluate the clinical characteristics of left ventricular fat replacement. Methods We identified 45 patients [28M/17F, mean age (51.9 ± 14. 7 )years] with left ventricular myocardial fat replacement ( CT value ≤ - 30 Hu) by cardiovascular CT. Results Among 45 patients, 25 patients[20M/5F, mean age (61.2 ± 10. 4) years] were diagnosed as coronary artery disease (CAD). There was 56% single-vessel disease, 20% double-vessel disease and 24% triple-vessel disease,true left ventricular aneurysm was detected in 3 patients and left ventricular thrombi in 1 patient, the dimension of left ventricle was (54. 5 ±9. 4) mm and the LVEF was (51.8 ± 13 ) % in CAD group. In this group, fat replacement occurred in the region of myocardial infarction and presented as curvilinear band in subendocardial region. The left ventricular wall thickness was lower than 5 mm in 21 cases. The location of fat replacement in CAD group is as follows: apical region in 18 patients, distal septal in 15 patients, distal anterior in 11 patients, mid-septal in 7 patients, mid-anterior in 7 patients and basal in 1 patients. The age of remaining 20 patients (8M/12F) without CAD were (57. 8 ± 13.3) years. In the group of non-CAD,dilated cardiomyopathy was diagnosed in 3 patients, atrial septal defect in 1 patient, rheumatic heart disease in 1 patient, there was no structural heart disease in the remaining 15 patients. The dimension of left ventricle was (51.1 ± 9. 1 ) mm and the LVEF was (59. 4 ± 13.9 )%. In non-CAD group, fat replacement mainly occurred in septal region, presented as curvilinear band in 17 patients and patch in 3 patients. The location of fat replacement in this group is as follows: mid-septal region in 11 patients, distal-septal in 10 patients and apical in 9 patients. The intramural fat replacement was detected in 14 patients: subendocardial fat replacement in 10 patients and both intramural and subendocardial fat replacement in 4 patients. Conclusions Left ventricular fat replacement could be documented in CAD patients, non-CAD cardiomypathy patients and in patients without structural heart disease. Left ventricular fat replacement often positioned in apical region in CAD patients as a consequence of infarct healing while mostly positioned in septal region in non-CAD patients, the definite clinical implication of left ventricular fat replacement in nonCAD patients remains to be clarified.

Objective To analyze the diagnostic feature, treatment and prognosis of patients with Cantrell syndrome. Methods Clinical manifestation, diagnosis, operation and follow-up data of 5 patients with Cantrell syndrome were summarized in this retrospective analysis. Results The age of the 5 patients was 7 days-76 years, definite diagnosis was made in 3 cases and 2 cases presented feature of incomplete Cantrell syndrome. Three patients with full Cantrell syndrome were correctly diagnosed before operation and confirmed by operation. One patient with incomplete Cantrell syndrome (two-vessel stenosis ) received bypass surgery. Another asymptomatic patient with incomplete Cantrell syndrome ( apical diverticulum of the left ventricle) does not need operation and is under observation. During follow-up, 1 patient died at 60months after operation and the remaining 4 patients are alive and well. Conclusions With the development of modern imaging technology, it becomes easy to make correct diagnose Cantrell syndrome before operation.Prognosis is fine post timely operation and related intervention.

Objective To analyze the cause of in-hospital death among acute myocardial infarction patients undergoing primary percutaneous coronary intervention ( PPCI ) in Beijing area to evoke better individualized preventive approach.Methods In-hospital mortality and causes were analyzed based on database from Beijing percutaneous coronary intervention registry study ( BJPCI Registry) in 2010.Results A total of 4660 PPCI patients from 48 hospitals were included.In-hospital mortality was 2.4％ ( n =110).Cardiogenic shock ( 39.1％,43/110 ),mechanical complications ( 28.2％,31/110 ) and interventionrelated complications [28.2％,31/110:procedure related ( n =28),drug related ( n =3 ) ] were the leading causes of in-hospital death. Five deaths was attributed to comorbidity related reason (4.5％,5/110).The in-hospital mortality had no significant difference among hospitals of different grade or total annual PCI (all P ＞ 0.05).In-hospital mortality was slightly higher in hospital with annual PPCI ＜ 300 than in hospitals with annual PPCI ≥ 300 ( 2.9％ vs.1.8％,P ＜ 0.05 ).Conclusion Cardiogenic shock,mechanical complications and intervention-related complications are the main causes of in-hospital death among acute myocardial infarction patients receiving PPCI.