0.05). Conclusion Isthmus is an essential structure to determine the size of femoral component. The dimension of isthmus correlates fairly well with the size of femoral stem. As a measurement to choose the appropriate femoral implant in total hip arthroplasty, the isthmus-method has the similar accuracy as the common templating.

Objective To establish a method for determination of 3’-chloromethylacetophenone in the air of ketoprofen workplaces. Methods The 3’-chloromethylacetophenone was sampled using activated carbon tube and then desorbed by ethyl acetate. All the components were determined by gas chromatograph with FID after complete separation with FFAP capillary column. Results This method presented a linear relation within 5-1 000 ?g/ml, r value was 0.999 2. The detection limit was 1.67 mg/m3. Desorption efficiencies were 92.2%-101.5%. The break through volume of 100 mg of activated carbon was 5.0 mg. Conclusions The present method is feasible for determination of 3’-chloromethylacetophenone in workplace air.

Objective To investigate the efficacy of percutaneous endoscopic laser-assisted discectomy (PELD) on L5S1 herniated nucleus pulposus by posterior paramedian trans-interlaminar approach (PEILD). Methods 168 consecutive patients underwent PEILD between May 2002 and December 2003, and were analyzed retrospectively. All cases were operated in outpatient department under local anesthesia in Wooridul Spine Hospital of Korea. Of the 168 cases, 92 were males, and 76 females with a mean age of 42.8 years (range,18 to 73 years ). The classification of herniated disc position related to spinal canal and pedicle, was central in 22, para-central in 120, foraminal in 23, and extraforaminal in 3. The average of iliosacral distance was 38.6 mm. "C" arm fluoroscopy was needed during operation. All of the patients were given conservative management for 6 to 8 weeks without relief of radiculalgia. The AP film was used to make sure to have enough working interlaminar space of posterior percutaneous approach for L5S1 herniated nucleus pulposus. Results The operative time was 30 to 90 mins with an average of 45 mins, and the mean hospitalization was 1.3 days. The average follow-up duration was 8.3 months (range, 5 to 19 months), and the patients were evaluated at 2 day, 2 week, and 1, 2, 6 and 12 month respectively. The clinical results were assessed by MacNab criteria, there were excellent in 43.5%, good in 47.0%, fair in 4.8%, and poor in 4.8%, and successful rate was 90.5%. 8 cases showed incomplete removal of the pathological disc on postoperative MRI without changes of symptoms and signs, then open laminectomy and microdiscectomy (OLM) were performed, including 4 of foraminal type, 3 of extraforaminal type and 1 of central type. 1 case displayed discitis, which was improved one month later by intravenous antibiotics, immobilization and bedrest. 26 cases showed transient postoperative paresthesia, improved by conservative treatment. Conclusion L5S1 disc herniation can be effectively treated with percutaneous endoscopic laser discectomy through posterior paramedian trans-interlaminar approach, which is easier for the patients with higher iliac spines.

砄bjective: To study the cause,clinical characteristics and treatment of zygomatic complex fractres(ZCF). Methods: Medical records of 79 cases with ZCF and followed up for 3 months to 2 years were reviwed and analysed. Reduction and cosmatic outcome were assessed by clinical evaluation and postoperative images. Results: Of the 79 patients, 87.3% were male, with the peak incidence in the age group from 20 to 40 years old(73.5%). 58.2% were due to traffic accidents, 15.2% to assalts. Incidence of malar asymmetry, enophthalmos, diplopia,limited mandiblar movement and malocclusion were 83.5%,24.1%,19.0%, 60.8% and 35.4% respectively. Of the nerve disturbances, 41.8% of the infraorbital and 13.9% of the facial nerve were involved. For the treatment the most freqently used approach was the coronal incision in combination with the maxillary vestibular incision(30.4%), followed by coronal incision alone(22.8%). 40.5% of the patients were treated by rigid internal fixation with titanium miniplates alone or combined with wire fixition(20.3%). All patients but five regained satisfactary facial appearance. Three patients still showed slightly post surgical enophthalmos and diplopia at a 6 month follow up. All of the patients with malocclusion regained their functional occlusion after treatment. Conclsions:A variety of techniques can be used in the treatment of ZCF. Among them the coronal incision in combination with other incisions or alone and rigid internal fixation can produce a satisfactary outcome.

Objective To establish a model of isoniazid induced necrosis and apoptosis in HepG2 cell and to observe the expressions of Fas/Fas ligand (FasL) in this model.Methods HepG2 cells were treated with different dosages of isoniazid (0,1,2,4,6 and 8 mg/mL) or blank control for 24 hours.Flow cytometer was used to observe the cellular morphology of the HepG2 cell.Annexin V/propidium iodide double staining and flow cytometry were employed to detect the necrosis and apoptosis of HepG2 cells.The expressions of Fas/FasL on the cells were also determined by flow cytometry.The data were analyzed by one-way ANOVA.The comparisons between the drug groups and the control group were performed by using Dunnett t test. Results The higher the dose of isoniazid (4,6,8 mg/mL) was,the more necrosis and apoptosis were observed.In the 4,6 and 8mg/mL isoniazid arms,the total mortality rates were all higher than the control group [(32.1 ±7.5)％,(34.9±8.1)％,(38.2±9.4)％ vs (7.2±1.5)％ respectively](t=4.62,5.14 and 5.75,respectively; all P＜0.01 ).The expression levels of Fas increased along with the dose of isoniazid increasing [(8.7±2.2)％,(11.5±2.8)％,(12.3±3.0)％ and (10.6±2.9)％ in isoniazid 2,4,6and 8 mg/mL arms,respectively],which were all higher than that in control arm [(3.1 ±0.8) ％](t=2.97,P＜0.05; t=4.46,P＜0.01; t=4.88,P＜0.01; t=3.98,P＜0.05).Furthermore,the expressions of FasL increased as well when the dose of isoniazid increased.The expression levels of FasL were (16.2±3.5)％,(21.7±4.8)％ and (18.7±4.9)％,respectively in isoniazid 4,6 and 8 mg/mL arms,which were all higher than that in the control group [(7.4±1.4)％](t=3.11,P＜0.01; t=5.06,P＜0.01; t=3.99,P＜0.05).HepG2 cell necrosis increased with isoniazid of 8 mg/mL.However,the increase of apoptosis was not observed.Conclusion Isoniazid can induce HepG2 cell necrosis and apoptosis,and the apoptosis may be related with the increased expressions of the Fas/FasL on the cells.

In anesthetic clinical teaching,Dalian Medical University Department of Anesthesiology pays more attention to humanity education and keeps improving teaching methods.It also adopts the methods of “the combination of the virtual and reality”,multiple apprentices,PBL and other ways to enhance students' operational skills development and promote their clinical thinking.

Objective To evaluate the variation of the blood flow in anterolateral femoral head by ligating different amount of extracapsular arteries. Methods Thirty six hips of 18 mongrel puppies were divided into A、 B、 C、 D and E groups. In group A, only one peripheral artery was ligated randomly. In groups B, C, D,and E, 2, 3, 4, and 5 peripheral arteries were respectively ligated. The blood flow in anterolateral femoral head in 18 mongrel puppies was measured before and after the ligation of the arteries by using of Hydrogen clearance technique. Results 1) The average blood flow in anterolateral femoral head was (0.1397?0.0515) ml?min-1?ml-1. 2) The mean blood flow in the anterolateral femoral head was slightly decreased after one to two of the extracapsular main arteries were ligated but there was no statistical difference. However, the mean blood flow was decreased significantly after more than three arteries were ligated. Conclusion 1) Hydrogen clearance technique is a quite simple, economic and reliable method for estimation of blood flow perfusion in various tissue. 2) The efficiency of blood supply of the anterolateral femoral head in puppies depends at least upon three of the major extracapsular arteries.

Chop and Grp78 genes are key genes in endoplasmic reticulum stress(ERS).To explore the gene alteration of Chop and Grp78 in embryo with spina bifida,we studied Chop and Grp78 gene expression by qRT-PCR in neural tube of rat with spina bifida in E12 and primary cultured neuroepithelial stem cells(NEP).We found higher expression of both Chop and Grp78 in neural tube of rat with spina bifida than normal rats.While Chop and Grp78 genes were up-regulated after NEP differentiated into neuron.The up-regulation of Chop and Grp78 genes in neural rube of rat and NEP might be involved in the abnormal apoptosis in spina bifida related with ERS.And we found the primary culture of NEP provided a good model for embryo early development research.

Hepatocellular carcinoma(HCC)has high incidence rate,recurrence rate after surgecal treat-ment,as well as fatality rate in China .HCC is not sensitive to radiotherapy or chemotherapy and no effective treat-ment is available for HCC patients at advanced stage .Sorafenib is the first effective molecularly targeted drug for the treatment of HCC,which represents a milestone in the treatment of HCC .However,it also shows drug resist-ance during clinical application .Therefore,it is important to investigate the mechanism of drug resistance and its molecular markers for HCC .In this review ,we summarize the current status of the studies in these fields .

Objective To construct pSUPER-SVV vector and obtain its expression in HeLa cells,in order to interfere the expression of survivin.Methods Oligonucleotide sequences specific for survivin were designed for RNA interference(RNAi),after annealed they were ligated to pSUPER.basic vector.After sequencing,the vector of pSUPER-SVV was transfected into HeLa cells by LipofectAmine.The expression of survivin was detected by RT-PCR and FCM.Results The RNAi expression vector of survivin was constructed successfully,which depressed the expression of survivin efficiently.Survivin was significantly depressed on the level of mRNA in HeLa cells compared with the control groups(P