1.Production and Characterization of Human CD27lg, CD40fg and CD95lg Fusion Proteins in Chinese Hamster Ovary Cell.
Bo Hyun CHO ; Yong Hoon CHUNG ; Yang Ja CHO
Korean Journal of Immunology 2000;22(4):253-264
No abstract available.
Animals
;
Asian Continental Ancestry Group*
;
Cricetinae
;
Cricetulus*
;
Female
;
Humans*
;
Ovary*
2.Characterization of cytoplasmic Form of Human CTLA - 4 Molecule.
Yang Ja CHO ; Yong Hoon CHUNG ; Hyung Soo HAN
Korean Journal of Immunology 1997;19(2):219-228
CTLA-4 (=CD152), a T cell activation antigen, has been known to be homologous to CD28 in its molecular and genomic structure. Both of these two molecules are sharing their counterreceptors, B7 (CDSO) and B7-2 (CD86) and are known to play a crucial role in T cell activation. In previous our study it was reported that there are 2 forms of CTLA-4 antigen in activated human T cells, 30 kD membrane-bound form and 34 kD cytosolic-sequestered form and the former was less than 5 % of total of this antigen induced. Aims of this study are to confirm previous finding by using flow cytometry and to characterize the cytoplasmic form of human CTLA-4 by using ultrafiltration and immunoprecipitation techniques. In PHA stimulated peripheral blood lymphocyte surface expression of CTLA-4 was less than 2.1% of any of CD4+, CD8+ and CD56+ subsets. And the 34 kD form of CTLA-4 was detected in CDS+ subset only. This discrepancy confirms that 34 kD antigen is the cytoplasmic form of human CTLA-4. In ultrafiltration and subsequent Western blot analysis study this 34 kD antigen was detected in >100 kD fraction only. And in non-reducing condition this antigen formed high molecular weght complex (MW > 350 kD). In immunoprecipitation study using anti-peptide A antibody it was found that this high molecular weight complex consists of the 34 kD cytoplasmic form of CTLA-4 and previously unknown 54 kD antigen and 46 kD antigen at 1:1:8-10 ratio. And none of these 3 molecules were identified in membrane fraction of activated human T cell. The result of this study implies that CTLA-4 molecule induced upon T cell activation mainly sequestered in cytoplasrn and another signal is necessary to target this antigen on the activated T cell surface.
Antigens, CD27
;
Blotting, Western
;
CTLA-4 Antigen
;
Cytoplasm*
;
Flow Cytometry
;
Humans*
;
Immunoprecipitation
;
Lymphocytes
;
Membranes
;
Molecular Weight
;
T-Lymphocytes
;
Ultrafiltration
3.Expression of Proinflammatory Cytokine Genes in Mouse Peritoneal Tissue Infected with Bacteroides fragilis.
Yang Ja CHO ; Jung Mogg KIM ; Won Seop KIM
Journal of the Korean Society for Microbiology 1999;34(3):253-264
OBJECTIVE: Bacteroides fragilis, normal colonic inhabitant, is the most frequently isolated anaerobes in infected tissues, particularly in intraabdominal abscesses. In the acute infection model with abscesses, the response to B. fragilis infection is characterized by infiltration of neutrophils and macrophages. This study was designed to determine whether proinflammatory cytokines could be upregulated in peritoneal tissue of B. fragilis-infected mouse model. METHODS: After C57BL/6 mice were infected with abscess-inducing encapsulated B. fragilis, RNA was extracted from the intraperitoneal tissues. Cellular RNA was also extracted from mouse peritoneal macrophages (MPM) and human peripheral blood mononuclear cells (PBMC) after infection with B. fragilis. Expression of various cytokine mRNA was assessed using RT-PCR and standard RNA. Each cytokine protein was also measured by ELISA. RESULTS: B. fragilis-infected intraperitoneal tissues showed upregulated expression of IL-1u, IL-6 and TNFu mRNA. Expression of IL-1u and TNFu mRNA and protein was significantly higher in MPM or PBMC infected with B. fragilis than in those without infection. However, expression of IL-6 mRNA and protein was not increased in MPM or PBMC infected with B. fragilis compared with those without infection. CONCLUSION: These results suggest that the cytokines can be involved in immunopathologic reactions of the peritoneal tissue infected with B. jragilis.
Abscess
;
Animals
;
Bacteroides fragilis*
;
Bacteroides*
;
Colon
;
Cytokines
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Interleukin-6
;
Macrophages
;
Macrophages, Peritoneal
;
Mice*
;
Neutrophils
;
RNA
;
RNA, Messenger
4.Expression of Cyclooxygenase - 2 in Intestinal Epithelial Cells in Response to Invasive Bacterial Infection and its Role of Epithelial Cell Apoptosis.
Jung Mogg KIM ; Shin Jae KANG ; Yang Ja CHO
Journal of the Korean Society for Microbiology 1999;34(5):479-489
Invasion of enteric bacteria, such as Salmonella and invasive E. coli, into intestinal epithelial cells induces proinflammatory gene responses and finally epithelial cell apoptosis. In this study, we asked whether invasive bacterial infection of human intestinal epithelial cells could upregulate cyclooxygenase-2 (COX-2) gene expression and whether increased COX-2 expression could influence intestinal epithelial cell apoptosis. Expression of COX-2 mRNA and prostaglandin (PG) E production were upregulated in HT-29 colon epithelial cells which were infected with S. dublin or invasive E. coli, as examined by quantitative RT-PCR and radioimmunoassay. Inhibition of COX-2 expression and PGE2 production using NS-398, a specific COX-2 inhibitor, showed a significant increase af epithelial cell apoptosis and caspase-3 activation in HT-29 cells infected with invasive bacteria. However, the addition of valerylsalicylate, a specific COX-1 inhibitor, did not change apoptosis in S. dublin-infected HT-29 cells. These results suggest that upregulated COX-2 expression and PGE2 production in response to invasive bacterial infection could contribute to host defense by inhibiting apoptosis of intestinal epithelial cells.
Apoptosis*
;
Bacteria
;
Bacterial Infections*
;
Caspase 3
;
Colon
;
Cyclooxygenase 2
;
Dinoprostone
;
Enterobacteriaceae
;
Epithelial Cells*
;
Gene Expression
;
HT29 Cells
;
Humans
;
Prostaglandin-Endoperoxide Synthases*
;
Radioimmunoassay
;
RNA, Messenger
;
Salmonella
6.Inflammatory Reaction in Intestinal Mucosa by Bacteroides fragilis Enterotoxin Stimulation: Regulation of Chemokine Gene Expression in Human Intestinal Epithelial Cells.
Jung Mogg KIM ; Soo Jin CHO ; Yu Kyoung OH ; Hee Bok OH ; Yang Ja CHO
Journal of Bacteriology and Virology 2001;31(2):113-121
No abstract available.
Bacteroides fragilis*
;
Bacteroides*
;
Enterotoxins*
;
Epithelial Cells*
;
Gene Expression*
;
Humans*
;
Intestinal Mucosa*
7.Change of serum ?-microglobulin, p24 antigen and CD4+ T lymphocyte in persons with human immunodeficiency virus infection after azidothymidine treatment.
Yung Kul CHO ; Yoo Kyum KIM ; Yung Oh SHIN ; Yang Ja CHO
Korean Journal of Infectious Diseases 1993;25(3):211-220
No abstract available.
HIV*
;
Humans
;
Humans*
;
Lymphocytes*
;
Zidovudine*
8.Respiratory Syncytial Virus Infections and Application of Nested Reverse Transcription-Polymerase Chain Reaction.
Kon Hee LEE ; Hae Sun YOON ; Kyu Man KEE ; Kyung Hee KIM ; Yang ja CHO
Journal of the Korean Pediatric Society 1995;38(11):1486-1497
No abstract available.
Respiratory Syncytial Virus Infections*
;
Respiratory Syncytial Viruses*
9.Prophylactic effect of interleukin-2 on lethality by gram-negative bacteria in mice.
Hyung Su HAN ; Jung Mogg KIM ; Yong CHOI ; Wang Soo HAN ; Yang Ja CHO
Journal of the Korean Society for Microbiology 1991;26(4):345-353
No abstract available.
Animals
;
Gram-Negative Bacteria*
;
Interleukin-2*
;
Mice*
10.Effect of electric shock stress and psychological stress of conflict stimulation on interleukin-2 production and lymphokine-activatedkiller cell cytotoxicity.
Dong Rag CHOI ; Jung Mogg KIM ; Yong CHOI ; Yang Ja CHO
Korean Journal of Immunology 1991;13(2):151-162
No abstract available.
Interleukin-2*
;
Shock*
;
Stress, Psychological*