Laparoscopic surgery for upper-third gastric cancer has gradually been accepted by experienced surgeons as the mature of this technique.Different from the standardized and programmed D2 lymph node dissection in Laparoscopy-assisted Billroth Ⅰ gastrectomy,the indications and methods for laparoscopic splenic hilar lymphadectomy in the upper-third gastric cancer remains controversial.Unsolved problems include joint organs resection,appropriate surgical approach selection and variable vascular anatomy of the splenic hilum.Meanwhile,the long-term efficacy and safety of laparoscopic splenic hilar lymphadectomy for the upper-third gastric surgery need to be confirmed by evidence-based medical trials.With the advance of the theory and clinical practice,laparoscopic splenic hilar lymph node dissection will continue to progress.

Objective To investigate the association of the SNP in miRNA146A with genetic prediposion and earlier recurrence after resection for hepatocellular carcinoma (HCC).Methods In the casecontrol study including 173 HCC cases,DNA were exacted from cancer tissue embedded with paraffin and were amplificated by PCR,SNP was explored in gene sequence of miRNA146a (385 base pair including extron).The outcome were analyzed with genetic prediposion and clinical features.Result Only hsa-mir-146a rs2910164 was found.The genetype frequence of C/C 、G/G and C/G at rs2910164 gene locus were separately 61 (35.3％),21 (15％) and 86(49.7％) in cases.Compared to G/G genetype,C/C and C/G genetype were danger factor to onset risk of HCC (OR =3.086,95％ CI:1.289-7.390) ; C/G was danger factor to earlier recurrence after resection(OR =8.179,95％ CI:2.248-29.759).Conclusion rs2910164 may be associated with genetic prediposion and earlier recurrence after resection of HCC in Jiangxi hans

Whole rice grain was dissected by hand into fractions of pericarp, ale-urone, scutellum, embryo and endosperm. For the convenience of having enough amount of samples for amino acids assay some of which were recom-bined and finally three parts, pericarp +aleurone layer, scutellum + embryo and endosperm, were obtained. Protein, lysine, methionine, threonine and valine contents were determined for each part. The data obtained indicated that although total weight of the first two parts accounted for only 7.27-8.43% of the whole grain, they contributed 14.5-15.8% protein, 29.0-30.5% lysine, 11.8% melhionine, 16.8% valine and 19.4% threonine, contained in the whole grain. During husking process it indicated that the lesser amounts of pericarp, aleurone, scutellum and embryo were retained and the larger percentage of protein and lysine was lost. This fact was identified either by a specially designed miller in the laboratory or a commonly used sand wheel miller in the factory. The suitable degree of milling for rice was discussed and it was proposed that 92% extration for rice rather rational.

Lysine and methionine contents were determined in proteins of 21 rice samples including 17 varieties grown in 11 provinces. Ranges of lysine and methionine contents on crude protein basis were 3.21-3.76% and 1.63-2.06% respectively. The lysine content was found to be negatively correlated with the protein content of rice, but no correlation was found in case of methionine when they were expressed on the basis of crude protein. The absolute amounts of lysine and methionine were positively correlated with protein contained in the samples. The importance of breeding rice variety with protein of high lysine content was discussed in relation to promoting the protein nutritive status in South China.

A convenient microbiological method with high accuracy for cystine assay was introduced. Common foods including cereals, beans, sweet potato, fishes and other aquatic products as well as meats were selected for cystine and methionine analysis. Based on the data obtained, a brief discussion was made on the way for resolution of sulfur amino acids problem inherently in our traditional soy-cereal based diet.

Activity and localization of y-glutamyl transpeptidase (?-GT) , ?-Naph-thyl acetate esterase (ANAE) , alkaline phospbatase (AKP) and glucose-6-phosphatase (G-6-P) in the epididymis of the rat has been investigated histo-chemically by light microscopy.?-GT activity was mainly localized on the supranuclear region and the ste-reocilia of the epithelial cells. The intensity in the epithelium of the segment Ⅱ is the highest of the rat epididymis. There was a gradual decrease in the intensity of this enzyme from segment Ⅲ through Ⅳ and it was absent from the segment Ⅳ. Activity of ANAE in the segment Ⅳ, Ⅴ and Ⅵ was higher, but that in the segment Ⅰ,Ⅱ and Ⅳ was less. ANAE activity was localized throughout the cytoplasm of the epithelial cells in the segment Ⅰ,Ⅲ, Ⅴ and Ⅵ. Whereas the activity in the segment Ⅱ and Ⅳ was localized on the basal region of the epithelial cells. AKP activity was present in the basement membrane of the epididymal epithelium and the bloodvessel wall of subepithelial connective, tissue of the epididymal duct. G-6-P was distributed homogenously in the cytoplasm of the epithelial cells throughout the epididymal duct.The possible function of these enzymes in the rat epididymis are briefly discussed.

Objective To evaluate the academic quality and characteristics of edition of the Chinese Journal of Geriatrics. Methods Within the database of Chinese Medical Citation Index(CMCI), the citation analysis was used to analyze the number and its distribution of the cited original articles published in the Journal 1999-2004. Results The average freguency for a single article cited by other researchers was 2.82, and there were more articles cited than other journals. The authors of these articles were distributed in the 30 province and municipalities, among which Beijing, Guangdong Province, Shanghai were in the front of Geriatrics research. There were 514 cited journals, and self-citing rate was 6.07%. Conclusions The Chinese Journal of Geriatrics has published high quality articles, and has its own edition characteristics to keep its steady level of research. It is one of the most important information resources for the geriatrics researchers and one of the most important medical journal.

"Resolving,espulsing and tonifying" three methods are the general outline of endotherapy in surgery of TCM.The author of this article thinks that pelvic inflammation and appendagitis can be classified in internal ulcer and vusceral carbuncle,and it can be treated by "Resolving,espulsing and tonifying" three methods.As the general programme of treating female inflammatory aphoria in different periods,the three methods can avoid exfetatio and promote the probability of pregnancy.Resolving method is applied in acute period of inflammatory aphoria,active period of chronic inflammatory and chronic pelvic inflammation when abdominal mass has formed but vital qi is not deficient.The pathologic character in this period is sufficiency of vital qi and excessive of pathogenic qi.Expulsing method is applied in inflammatory aphoria patients whose pathogenic has not been cleared but the vital qi has been harmed,at this time damp heat pathogen poison remain in uterus meridians and the nvital qi has no power to resist pathogen,and whose corporeity is always week and infects pathogen poison which results in the course of diseases being repeated and prolonged.Tonifying method is applied after the resolving and espulsing methods have been used.In this period,damp heat stasis have been cleared,inflammation and inflammatory matters have been absorbed,disease has been improved and patient has had condition to be pregnant.Tonifying method can promote human generative function and improve the possibility of conception.

OBJECTIVE: To study the absorption and transport of pachymic acid (PA) isolated from the sclerotium of Poria cocos (Schw.) Wolf. in human intestinal epithelium. METHODS: By using Caco-2 (the human colonic adenocarcinoma cell lines) cell monolayers as an intestinal epithelial cell model, the permeability of PA was studied from apical side (AP side) to basolateral side (BL side) or from BL side to AP side. The PA was measured by reversed-phase high performance liquid chromatography coupled with UV detector at maximum absorption wavelength of 210 nm. Transport parameters and apparent permeability coefficients (Papp) were then calculated and compared with those of propranolol and atenolol, which were the transcellular transport markers for high and poor permeability respectively. RESULTS: The Papp values of PA were (9.50+/-2.20) 10(-7) cm/s from AP side to BL side, and (11.30+/-5.90) 10(-7) cm/s from BL side to AP side, respectively. Under the condition of this experiment, the Papp values were 1.45x10(-5) cm/s for propranolol and 4.22x10(-7)cm/s for atenolol. CONCLUSION: PA is transported through the Caco-2 cell monolayer in a concentration-dependent manner and the transport was linear with time. The absorption in apical to basolateral direction and secretion in basolateral to apical direction were poor and their Papp values were comparable to atenolol. Besides passive diffusion of PA, ATP is partially involved in its transport.

Objective To analyze the clinical effects, as well as its advantages and disadvantages, of laparoscopic surgery for appendicitis in obese patients. Methods Clinical data of 80 obese patients receiving appendectomy, including 38 cases of open appendectomy and 42 cases of laparoscopic appendectomy, were analyzed retrospectively.The operative time, blood loss, analgesic and antibiotic use, postoperative fever, postoperative complications, length of hospital stay, and hospitalization cost were compared between the two operative methods. Results Conversions to open surgery were required in 2 patients in the laparoscopic group. There was no significant difference in operative time between the 2 groups (P >0.05).Compared with the open group, the laparoscopic group had less blood loss [(14.98 ±12.77) ml vs.(31.58 ±19.00) ml, t=-4.550, P=0.000], shorter time of postoperative antibiotic use [(2.7 ±1.0) d vs.(4.1 ±1.2) d, t=-5.470, P=0.000], less postoperative analgesics needed [5.0%(2/40) vs.26.3%(10/38),χ2 =6.802, P=0.009], less drainage [2.5% (1/40) vs.18.4% (7/38), χ2 =5.367, P=0.021], less postoperative fever [5.0%(2/40) vs.23.7%(9/38),χ2 =5.616, P=0.018], less postoperative wound healing [5.0%(2/40) vs.21.1%(8/38),χ2 =4.493, P=0.034], and shorter hospital stay [(5.9 ±3.2) d vs.(8.7 ±4.1) d, t=-3.345, P=0.001], but the higher cost of hospitalization [(7800 ±396) yuan vs.(4914 ±434) yuan, t=30.716, P=0.000]. Conclusion For obese patients with appendicitis, laparoscopic appendectomy has less surgical trauma, faster recovery, less postoperative pain, fewer complications,and shorter hospital stay, being a preferred method of treatment.