High-consuming group is a special group which has good economic base, differmental culture and relgional belif customs. These patients alwanys need high quality medical service. How to integrate with these characteristics and make the nursing model of making patients' belife, moral ideas respcted, and rights proteded is a difficult problem. The author put forward we should rule nurse staff' s ethics and actions, provide a medical envionment from which can meet high-consuming patients physical and mental needs.

Objective To investigate the epidemiological characteristics of imported malaria in Hailing District ,Taizhou City,so as to provide the evidence for formulating the effective prevention and control strategy. Methods The network reports, questionnaires and epidemic reports of imported malaria cases in Hailing District from 2010 to 2015 were collected and analyzed retrospectively. Results From 2010 to 2015,totally 46 cases of imported malaria were reported,including 40 cases of falci?parum malaria(86.96%),4 cases of ovale malaria(8.70%),1 case of quartan malaria(2.17%)and 1 case of multi?infection of falciparum malaria and tertian malaria(2.17%). No seasonal trend of time of the disease onset was observed. All the patients were return abroad with clear residence histories in malaria epidemic areas. The average time from disease onset to definite diag?nosis was 2 d and 8 patients were definitely diagnosed within 24 h. All the 46 patients were cured. Conclusion All the malaria cases reported were imported cases in Hailing District in the last five years. Effective prevention and control measures are the key to the malaria elimination.

Objective To determine the mercury in seven kinds of algal plant drugs.Method The mercury in the samples was determined by the atomic fluorescence spectrometry after been digested by microwave.Results The detection limit of mercury was 0.045?g?L~(1).The relative standard deviation was 1.65% and the average recovery was 97.77%.Conclusion The method had the advantages of simple operation,higher precision,higher sensitivity and repeatability and was suitable for the determination of mercury in the algal plant drugs.

Objective To understand the current status of Oncomelania hupensis snails and the change rules,so as to pro?vide the evidence for formulating the snail control strategy. Methods The data of snails were collected and the factors influenc?ing the changes of snail status were investigated,and all the data were analyzed in Hailing District from 2007 to 2014. Results There were 18 environments with snails in 17 villages of 3 townships in Hailing District from 2007 to 2014. The snail area was 7.832 4 hm2. Among these environments and snail areas, 6 environments and 2.134 5 hm2, snail area were detected by the village snail investigation groups, and 12 environments and 5.697 9 hm2 snail area were detected by the township snail investigation groups. In addition, in 5 non?endemic villages, 3.126 3 hm2 snail area was found. In 2008, 2012 and 2013, a total of 2.274 0 hm2 snail area was found again. Conclusions In recent years,the snail situation rises again after schistosomiasis transmission has been interrupted. Therefore,this situation should be paid attention to.

Objective To study the effect of chitosan on the growth of the in vitro cultured retinal nerve cells. Methods The retinal nerve cells of SD baby rats were cultured in vitro. The effects of chitosan at 0.065%,1.25%,2.5%,5%,10%,15% on the growth of the retinal nerve cells were measured by MTT method. The blank control group was created. Results 1.25%,2.5%,5%,and 10% chitosan could obviously promote the growth of retinal nerve cells,and 10% chitosan was the optimal concentration for the growth of nerve cells. Conclusion Chitosan can effectively promote the growth of retinal nerve cells,and it is necessary to undertake the further study of Chitosan.

Immunohistochemistry examination showed that SOX-2 was expressed in all 23 cases of odontogenic cyst and in 2 cases of poly-cystic ameloblastoma.

BACKGROUND:Closed reduction and internal fixation are mostly used to treat fracture dislocation of tarsometatarsal joint, but cannot obtain satisfactory repair effect. Redisplacement easily appeared after local sweling subsidence. Therefore, open reduction and internal fixation are actively used in the clinic to treat fracture dislocation of tarsometatarsal joint so as to restore foot function and to elevate quality of life. OBJECTIVE:To explore the clinical effect of open reduction and cannulated screw and Kirschner wire fixation for fracture dislocation of tarsometatarsal joint. METHODS:A total of 42 patients with fracture dislocation of tarsometatarsal joint in the 88 Hospital of Chinese PLA from September 2012 to September 2013 were selected and were given open reduction and cannulated screw and Kirschner wire fixation. After treatment, the recovery of patients was observed, and ankle hindfoot function was assessed with American Orthopaedic Foot and Ankle Society ankle hindfoot score. RESULTS AND CONCLUSION:A total of 42 patients were folowed up for 1-12 months. American Orthopaedic Foot and Ankle Society ankle hindfoot score results showed that there were excelent in 30 cases, good in 10 cases, and poor in 2 cases, and the 2 patients were subjected to high energy soft tissue injury. 37 patients in 4-6 months after operation started weight-bearing walking. Three patients had mild pain, a certain functional limitation, but could maintain normal walking gait. The remaining two patients complicated with traumatic arthritis, and were unable to do normal weight-bearing walking. These results show that open reduction and cannulated screw and Kirschner wire fixation can achieve good effect of anatomical reduction of fracture dislocation of tarsometatarsal joint, and improve patient’s ankle function.

BACKGROUND:With the in-depth study of cancer stem cels, increasing evidence has shown that cancer stem cels are the reason for cancer metastasis and recurrence, and therefore it is of great significance to isolate and identify cancer stem cels that can elucidate the pathogenesis of cancer and development of anticancer drugs.
OBJECTIVE: To isolate and culture gastric cancer stem cels and then to detect its biological characteristics.
METHODS: Cancer tissues from 16 patients with gastric cancer were cultured using adherent method and enzymic digestion methods to isolate gastric cancer stem cels. The cel morphology was observed under inverted microscope to drawn out growth curves. Osteogenic and adipogenic ability of cels were also detected.
RESULTS AND CONCLUSION: Gastric cancer stem cels were isolated successfuly by both two methods. Under the microscope, the cels were fusiform- or polygon-shaped. At confluence, the cels grew in a vortex or radial manner. The cel growth curve showed that 1-3 days were latent period, 4-9 days were logarithmic growth phase, and over 10 days were growth plateau phase. Flow cytometry results showed that passage 3 gastric cancer stem cels highly expressed CD90, CD29, CD44, but lowly expressed CD34, CD45 and HLA-DR. After osteogenic induction, calcified nodules were clearly visible in the gastric cancer stem cels at passage 3; after adipogenic induction, bright and tiny fat droplets were seen in the cytoplasm. These findings indicate that cancer stem cels exist in the gastric cancer tissues, and have similar morphology, biological property and multipotent differentiation ability to normal cels, which are probably involved in gastric cancer occurrence and development.

Objective To observe the MSCT features of invasive pulmonary tuberculosis,and to investigate its effect on the prognosis.Methods 60 patients with invasive pulmonary tuberculosis were selected as the study subjects.The gold standard was in sputum culture and histopathology.The clinical value of MSCT in the diagnosis of invasive pulmonary tuberculosis was evaluated.The distribution and morphological characteristics of the lesions in the invasive pulmonary tuberculosis were observed and analyzed.The patients were divided into the treatment group and the death group.The morphological changes of lung lesions were compared between the two groups.Results In 60 patients,a total of 45 patients with invasive pulmonary tuberculosis were diagnosed,MSCT of infiltrating pulmonary tuberculosis diagnostic sensitivity,specificity,positive predictive rate,negative predictive rate,correct rate,misdiagnosis rate,missed diagnosis rate,diagnostic accordance rate were 93.3%,80.0%,93.3%,80.0%,73.3%,20.0%, 6.7%,90.0%,respectively.On the lung multiple patchy shadow,real change shadow,multiple nodules,cavity shadow, on the unilateral lung syndrome,and branch sign and halo sign is the main sign of invasive pulmonary tuberculosis. Before treatment,MSCT signs of deterioration of chest between the treatment group and death group had no statistically significant difference(P>0.05).After 4 weeks of treatment,the proportion of effusion lesions in the treatment group was significantly decreased compared with before and during the same period of treatment(37.5%vs 76.9%;3.1%vs 92.3%),the differences were statistically significant(χ2 =5.750,16.541,all P<0.05).After 8 weeks of treat-ment,the proportions of effusion,nodules and cavity lesions in the treatment group were significantly lower than those in the deteriorated group(3.1% vs 92.3%;0.0% vs 15.4%;6.2% vs 38.5%),the differences were statistically significant (χ2 =16.541,5.152,7.302,all P<0.05).Conclusion Morphological changes of MSCT in invasive pulmonary tuberculosis have certain characteristics.In the diagnosis,differential diagnosis and therapeutic evaluation of invasive pulmonary tuberculosis,MSCT has high value.

Objective To investigate clinical value of povidone -iodine disinfection for prevention of postpartum endometritis and abdominal incision infection in cesarean, to provide a reference for clinical treatment. Methods 160 cases of puerpera women were selected, all of them underwent uterine segment cesarean section. According to the digital table,they were divided into control group and observation group,80 cases in each group.In the observation group previous cesarean group remained untreated 30min before cesarean vaginal experiments 0.4%povidone-iodine for disinfection.The maternal postpartum endometritis incidence,abdominal incision infection rate, visual analogue scale,leukocyte index WBC,erythrocyte sedimentation rate index ( ESR) ,C-reactive protein ( CRP) were compared in the two groups.Results There were 32 cases of postoperative endometritis,12 cases (15.00%) occurred in the observation group, 20 cases ( 25.00%) occurred in the control group, the overall incidence of endometritis between the two groups had statistically significant difference (χ2 =5.945,P=0.025);endometritis 5cases (17.86%) premature rupture of membranes occurred in the observation group,the control group had 10 cases (31.25%),the difference was statistically significant (χ2 =6.565,P=0.009).Postpartum WBC,ESR,CRP in the observation group were significantly lower compared to the control group,the differences were statistically significant (t=5.045,P=0.007;t=7.945,P=0.001;t=8.676,P=0.000).In the observation group,postoperative abdominal incision infection rate was 3.75%,compared with the control group,there was no significant difference (χ2 =1.021, P=0.124).The VAS score of the observation group was (3.94 ±0.74),which was significantly lower than (6.25 ± 1.05) of the control group,the difference was statistically significant (t=6.331,P=0.010).Conclusion Previous cesarean vaginal povidone-iodine disinfection is effective in preventing postpartum endometritis,but can't help to low abdominal incision infection or improve wound healing.