Objective To analysis the hot spots of innovative technology of Chinese medicine industry in Shanghai during the recent years.Methods Patent Database of State Intelletual Property Office was retrieved by IPC class numbers combined with keywords and the IPC classification of the Chinese medicine patents in Shanghai area during 2002-2013 were under statistical analysis.Results The hot spot distribution of therapeutic activity of TCM preparations:Antineoplastic is the most important activity of Chinese medicine development, followed by the activities of treatment of inadequate blood flow or cardiovascular system diseases, such as hyperglycemia, high cholesterol, and so on. The activity of treatment of the digestive tract or digestive system diseases, followed by the activities of anti infection, antiviral, antibacterial, anti-inflammatory treatment, etc. The hot spot distribution of dosage:Researches on new dosage of Chinese medicine were mainly reflected in the improvement of traditional dosage form and development of new formulations. The capsules, tablets, oral liquid, and injections were the new dosage forms under the most researches. The distribution of medical resources:Medicinal plants or animals mainly came from “angiosperm”. Medicinal animals were less than medicinal plants, which mainly belonged to insecta.ConclusionThe hot spots of innovative technology of Chinese medicine industry in Shanghai are mainly distributed in the medicine of anti-tumor, cardiocerebral vascular diseases, digestive system diseases and so on in recent years. The researches of new formulation focus on the traditional dosage form improvement and new formulations development. “Angiosperm” is the key to the development of medicinal plants.

BACKGROUND: The coating induced by conventional simulated body fluid (SBF) and 1.5-folds SBF as biomimetic method has the shortcomings of slowly growth with weeks or months growth cycle. OBJECTIVE: To evaluate Ti-6Al-4V alloy surface deposition of hydroxyapatite coating induced by 3-folds SBF. DESIGN, TIME AND SETTING: In vitro biological analysis. The experiment was performed at the Institute of Metal Research, Chinese Academy of Sciences from November 2008 to March 2009. MATERIALS: Ti-6A1-4V alloy was supported by Boda Metel Materials Co., Ltd. METHODS: The pretreated Ti-6Al-4V alloy was immersed in 37 'C 3-folds SBF. The SBF was replaced every 24 hours for 3 days. MAIN OUTCOME MEASURES: The morphology, phase and elemental composition of calcium phosphate coating were analyzed by scanning electron microscopy, X-ray diffraction and infrared spectroscopy analysis. RESULTS: The scanning electron microscopy exhibited that the surface of Ti-6A1 -4V alloy was covered with deposited film with granular crystal on local areas at day 1 after immersion. The deposited film covered surface of Ti-6A1-4V alloy at day 3 after immersion, and granular or scale-shaped crystal could be seen. The crystal deposition displayed sustained growth with certain area as center. The product comprised Ca, P, O, C elements without Al, V. X-ray diffraction and infrared spectroscopy analysis revealed that the main component of the coating was hydroxyapatite. CONCLUSION: The 3-folds SBF can accelerate the deposition as well as shorten formation duration of hydroxyapatite coating on Ti-6A1-4V alloy.

BACKGROUND:At present, the study to balance ability of functional ankle instability patients is less. The
balance ability deficiency may also be one of the important reasons for ankle repeated injury, so the research can effectively reduce the damage probability.
OBJECTIVE:To explore the static balance ability characteristics of functional ankle instability patients.
METHODS:Ten male subjects with unilateral functional ankle instability were enrol ed as the experimental group in the study, and other 10 healthy match-paired subjects were served as the control group. Their static balance ability was measured under double feet standing and single foot standing using win-pod system, the double feet testing lasted for 30 seconds and the single foot testing lasted for 10 seconds. Evaluation parameters of balance ability were obtained.
RESULTS AND CONCLUSION:During double feet testing, there were no significant differences in balance
ability parameters between the experimental group and the control group whether eyes be opened or closed (P>0.05), but the index values of double feet testing were obviously less than those of single foot testing. So we
concluded that in the double feet standing, there was no significant difference in the static balance ability between functional ankle instability patients and normal persons. Moreover, the static balance ability of double feet was
better than that of single feet. In the single foot standing of the affected foot, the static balance ability of functional ankle instability patients was slightly worse than that of the normal persons, especial y with eyes closed.
Comparison of the average swing between the X-axis and Y-axis found that in the process of maintaining balance posture, the swing direction of the body center of gravity was mainly in the anteroposterior direction.

Objective To explore whether the inhibited expression of fibrocystin by RNA interference can increase epidermal growth factor (EGF)-induced cell proliferation and its possible mechanism . Methods A stable PKHD1-silenced HEK 293 cell line was established . Cell proliferation rate, intracellular Ca2+ concentration and extracellular signal-reguhted kinase 1/2(ERK1/2) activity were assessed after treatment with EGF, verapamil and Bay K8644 . Results The proliferation rate of PKHD1-silenced HEK-293 cells was found to be significantly higher after EGF stimulation compared to the control HEK 293 cell (231 .5% vs 152 .8%, P＜0 .01) . PKHD1-silencing lowered the intracellular Ca2+ concentration and caused EGF-induced ERK1/2 overactivation in the cells(P＜0 .01 ) . When cells were treated with verapamil for 4 hours to lower the intracellular Ca2+ concentration, the cell proliferation rate was significantly increased after 20 ng EGF for 24 hours . The verapamil treatment increased the level of activated ERK1/2 in EGF-treated cells . An increase of intracellular Ca2 + in PKHD1-silenced ceils repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation . Conclusions Inhibition of fibrocystin can cause EGF-induced excessive proliferation through decreasing intracellular Ca2+ resulting in EGF-induced ERK1/2 activation . The loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD through modulation of intracellular Ca2+ concentration .

[ Objective ] To evaluate the significance of high-frequency ultrasonography (HFU) combined with color Doppler velocimeter (CDV) for the differential diagnosis of breast masses (BM) . [Methods] HFU and CDV findings in 113 cases of BM were compared with their pathological findings. [Results] There was a certain relationship between HFU findings and pathological findings. The detectable rate for the blood flow accounted 97.80% in breast cancer examined with HFU, resistance index (RI) being 0.77?0.06; detectable rate for benign BM was 61.76%, RI being 0.52?0.05. The difference was significant between the two detectable rates (P

Objective To investigate the clinical characteristics, pathological and biochemical featur es of paraneoplastic syndromes (PNS) of hepatocellular carcinoma (HCC). Methods The clinical data of 624 HCC patients (the ratio of male to female 5.3∶1) ident ified by pathology were retrospectively reviewed. Results Among the patients with HCC, 32.2% (201/624) had PNS, the ratio of male to femal e was 4.6∶1. PNS mainly manifested as six clinical abnormalities: hyperchol esterol emia (15.9%), hypoglycemia(8.2%), thrombocytosis(7.7%), leucocytosis(4.8%), eryt hrocytosis(3.4%), hypercalcemia (1.0%). The carcinoma of PNS originated in multi si tes, they mainly located at the right lobe of liver, whilst 47.5% of patients wi th PNS were involved in two lobes. The level of ?-glutamyltranspeptidase in PN S was statistically higher than that free of PNS controls (261.2?224.0 vs. 165 .8?152.2, P

Objective To evaluate the effect of intra-arterial infusion of basic fibroblast growth factor(bFGF) on improving neovascularization, vascular perfusion and the function of partially ischemic limbs of rabbits. Methods Twenty-seven New Zealand male rabbits were selected. Partial ischemia model was induced by surgical ligation of the primary branches of right femoral artery in each animal, and the left hind limb of each animal was served as a nonischemic control. Then, 27 rabbits were randomly assigned to three groups: intra-arterial (IA) infusion of bFGF (n=9), intravenous (IV) infusion of bFGF and IA infusion of saline (n=9). Infusion was separately performed immediately after vascular ligation, 8th and 15th days post-surgery with 10 ?g (4 ml) of bFGF per-time (or the same volume of saline). The differences between three groups and between ischemic and nonischemic limbs of the same group were compared and evaluated by the following indexes: (1) vessel section count (VSC), vessel section surface area (VSS) and vessel section perimeter (VSP) in the field of ischemic muscle tissues taken at 22nd day postoperatively; (2) capillary refilling time of ischemic limbs; and (3) functional and trophic changes of ischemic limbs. Statistical differences were evaluated by one-way ANOVA and T test. Results VSC, VSS and VSP of the IA-bFGF group were significantly increased than those of the IV-bFGF and IA-saline groups (P

Objective In order to compare of consistency of results between URIT‐1500 and AIKELAI AX‐4030 urine drychemistry analyzers and for satisfy the need of urine routine test will choose in the reference range of the optimal urine drychemistry analyzers .Methods During the testing time from March 6 ,2015 to April 4 ,2015 ,random have been collected 378 cleaning midstream urine specimens form Luzhou medical college affiliated hospital were detected by the two kinds of urine drychemistry analyzers in parallel .Positive detection rates of each project were analyzed statistical by chi‐square test and counting accordance rate ,general accordance rate ,and the kappa value completely .Results The percentage of variance difference of specific gravity(SG) between the two analyzers was 0 .564% (<1% );Positive detection rates(P value) of WBC ,BIL ,PRO ,BIL between the two analyzers were<0 .05 ,the results were statistical significantly ;Positive detection rates(P value) of GLU ,NIT ,KET ,URO were>0 .05 ;the general accordance rate of LEU ,GLU ,NIT ,KET ,pH were>90% and BLD ,PRO ,URO ,BIL were>70% .Com‐pletely accordance rate except GLU ,NIT ,KET were>90% and the rest of six projects trend lower than normal rate .The kappa value of LEU ,BLD ,GLU ,KET ,URO ,BIL were>0 .40 and PRO ,pH were>0 .30 .Conclusion There is a poor consistency be‐tween the two kinds of urine drychemistry analyzers in the same test condition .It can not to use two different kinds of analyzers in same hospital and should choosing the optimal urine drychemistry analyzers in reference range need on the basis of the particular situations of hospital .Urine drychemistry analyzers is used only for screening ,and the results should be reviewed by urine sediment microscopy in order to meet clinical needs .

Objective To explore the effect of the application of cervical loop electric knife (LEEP) in the patients with cervical micro invasive cancer.Methods Clinical data of 66 patients with minimal invasive carcinoma diagnosed by LEEP cone resection in our hospital from June 2013 to June were retrospectively analyzed. Preoperative and postoperative patients with LEEP were compared with 66 patients who underwent biopsy with biopsy, the diagnosis of micro invasive carcinoma was compared with LEEP cone resection.Results The results of colposcopy biopsy were detected in 2 cases of LSIL, 60 cases of HSIL and 1 cases of AIS, 3 cases were diagnosed as cervical microinvasive carcinoma or suspicious cervical microinvasive carcinoma, the sensitivity was 4.54%, the misdiagnosis rate was 95.46%; LEEP conization were detected in 1 cases of LSIL, 44 cases of HSIL and 1 cases of AIS, 20 cases negative margin. There was no significant difference in the thickness and area of the resected tissue between the three groups (P > 0.05). The depth of the resected tissue was significantly higher than that in the HSIL and AIS group, the HSIL and AIS groups were significantly higher than the LSIL group, the difference was statistically significant (P < 0.05). Interstitial infiltration depth is less than or equal to 1mm group, the 1 depth of stromal invasion than 3mm group and 3 < rate were 15.00%, 19.51% and 20.00% of the remaining lesion depth of stromal invasion than the patients in the 5mm group after operation, there was no statistically significant difference (P > 0.05). Cut edge positive group, positive margin group and interstitial fiber positive margin group of patients with postoperative residual disease rates were 25.00%, 15.38% and 23.80%, the difference was not statistically significant (P > 0.05).Conclusion The rate of missed diagnosis of small invasive cervical cancer is higher, and the diagnosis rate can be improved by the combination of LEEP and cone resection.

AIM: To prepare the asperosaponin—phospholipid compound to improve the bioavailability of asperosaponin in rat intestine in situ. METHODS: Ratio of asperosaponin to phospholipid was chosen as index to screen the optimal proportion based on the orthogonal design.Rat intestine perfusion was use to compare aspersaponin absorption under with and without phospholipid. RESULTS: Asperosaponin—phospholipid compound showed the markedly improvement in absorption capacity and absorption rate. CONCLUSION: The experiment recommends that asperosaponin in the combination with phospholipid is better than only used asperosaponin concerning bioavailability.