The strategies for constructing pharmaceutical subject repository union in China and the development trend of pharmaceutical subject repository union were studied form the aspects of its cooperation models, platform building, resources acquisition and organization, access policy formation;copyright, quality control, and fund source.The cooperation models were divided into centralized storage model, distribution acquisition model and hierarchical construction model.Pharmaceutical subject repository union platform was built according to the different cooperation models.

Objective:To evaluate the emergency diagnosis of severe myocardial contusion. Methods:Thirty patients with serious blunt chest trauma underwent sonourethrography,all of whom underwent conventional myocardial markers simultaneously.All the observation time phases were set as 1 h,2 h,4 h and 8 h after injuries. Results:Both the concentrations of cardiac troponin T (cTn-T)and cardiac troponin (IcTn-I)at 8h after injuries were significantly higher than those at 1h after injuries(P

Objective To study chronic bronchitis rats oxidant/antioxidant related indicators Ho flavored Dingchuan Decoction.Methods 50 SD rats were adopted improved smoked method to make chronic bronchitis model.From the 20th day of the modeling,blank control group and model control group gavage were treated with saline.The positive control group were treated with Cough Mixture.The experimental groupwere dealed with Ho flavored Dingchuan Decoction high,medium and low-dose.20 day later,the cotent of SOD,CAT,MDA in rat serum,lung tissue and balf were observed.Results Compare to blank control group,the cotent of SOD[(188.11 ± 9.37)U/ml、(276.11 ±30.28) U/ml,(8.80 ± 1.49)U/ml],CAT[(9.40 ±2.04)U/ml,(26.57±4.26)U/ml,(1.58±0.26) U/ml] in rat serum,lung tissue of the experimental group significantly reduced (P ＜0.01) ; MDA [(5.64 ± 0.52) μmol/L,(6.32 ± 2.10) μmol/L,(28.08 ± 2.43) μ mol/L) increased (P ＜0.05 or P ＜ 0.01).Ho flavored Dingchuan Decoction can effectively reverse these changes,and showed a significant dose-dependent.The Ho flavored Dingchuantang,in middle and high-dose serum SOD [(253.05 ± 7.81) U/ml,(256.92 ± 5.37)U/ml],CAT [(14.86 ± 2.49)U/ml,(18.22 ± 2.65)U/ml] higher than those of blank control group.And the Ho flavored Dingchuantang,in middle and high-dose lung tissue SOD [(470.91 ± 11.46)U/mgprot,(482.91 ± 14.32)U/mgprot),CAT [(46.87 ± 3.75)U/mgprot,(55.49 ±3.33)U/mgprot] higher than those of blank control group.Content close to the normal level.Conclusion The Ho flavored Dingchuan Decoction has significantly reduce the oxidative stress in chronic bronchitis.

AIM: To investigate the clinical effect of compound anisodine on patients with paralytic strabismus and the influence on malondialdehyde ( MDA ) and superoxide dismutase ( SOD) .
●METHODS: Seventy cases diagnosed with paralytic strabismus from Jun. 2008 to Dec. 2014 were selected as treatment group; another 70 cases with paralytic strabismus from Oct. 2000 to Jan. 2008 were chosen as control group. The control group was given with conventional therapy, and treatment group was additionally injected with compound anisodine subcutaneously on the basis of the control group. Treatment lasted for two courses about 28days. The effect of compound anisodine on patients with paralytic strabismus was evaluated and the serum level of MDA and SOD was also measured before and after treatment.
●RESULTS: ln treatment group, 54 cases were cured (77%), 14 cases improved (20%) and 2 cases had no response ( 3%) and total efficiency reached to 97%. ln control group, 32 cases were cured ( 46%) , 21 cases improved ( 30%) and 17 cases had no response ( 24%) and total efficiency was 76%. The cure rate and total efficiency of treatment group were significantly higher than that of control group (P<0. 05). Before treatment, the level of SOD and MDA was equal in two groups. After treatment, increased SOD level and decreased MDA level was observed in both groups. However, compound anisodine were significantly increased SOD level and reduced MDA level when compared with conventional therapy (P<0. 05).
●CONCLUSION: The compound anisodine shows the beneficial effect on patients with paralytic strabismus. Compound anisodine may exert the effect via increasing SOD and reducing MDA.

PI3K/Akt pathway is a biological signal transduction pathway activated by phosphatidylinositol 3-kinase (PI3K).It plays a key role in cell proliferation,cell-cycle regulation,apoptosis initiation,and angiogenesis.In addition,the PI3K/Akt pathway is closely associated with the protection mechanisms of central nervous system damage.In-depth study of PI3K/Akt,downstream molecules and their regulation mechanisms,can provide some new ideas and methods for the treatment of brain injury.

BACKGROUND:The quantity and function reduction of circulating endothelial progenitor cells(CEPCs)indicate that vascular endothelial repair capacity reduces in hypertension patients,but the impact of statins on the CEPCs is still not very clear.OBJECTIVE:To investigate the effects of atorvastatin calcium on endothelial cells and CEPCs in renovascular hypertensive rats.DESIGN,TIME AND SETTING:Randomized controlled animal experiment was performed in Chongqing Key Laboratory ofNeurology from June 2008 to Febmary 2009.MATERIALS:Totally 24 SD male rats of SPF grade,and atorvastatin calcium was produced by Lipitor,Pfizer Inc.with batch number of 65837003.METHODS:The renovascular hypertension was induced with 2-kindey and 1-clip method in 16 SD rats,which were then randomly divided into two groups:hypertensive group(n=8)and statins group(n=8).control group(n=8)underwent the same operation,without silver slip.At 4 weeks after model construction,statins group was treated with atorvastatin calcium MAIN OUTCOME MEASURES:At 4 and 12 weeks of the experiment.three groups of rats were measured blood pressure and blood lipids;at 12 weeks,the thoracic aortic endothelial cells continuity was investigated through hematoxylin-eosin stain;the quantity,proliferation,adhesion and migration capacity of CEPCs were determined.RESULTS:At 4 and 12 weeks of the experiment,there was no significant differences in the systolic pressure between statins RESULTS:At 4 and 12 weeks of the experiment,there was no significant differences in the systolic pressure between statins group group and model group,which were dramatically high than control group(P＞0.01):the blood lipids in the statins group was not changed.In the model group,rat aortic endothelial cells were greatly damaged;the injury of CEPCs was dramatically relieved in the model group.Compared with statins group and control group,the number of CEPCs was significantly decreased,the capacities of proliferation,adhesion and migration were also significantly reduced in the model group(P＜0.01);moreover,statins group was inferior to control group in terms of these capacities.with significant differences among three groups(P＜0.01).CONCLUSION:Renovascular hypertensive rats thoracic aortic endothelial calls are severely damaged,CEPCs decline in the humber and function;atorvastatin calcium can increase the number of CEPCs and improve the function of CEPCs.

Triple negative breast cancer (TNBC) is characterized by the lack of expression of hormone receptors, as well as human epidermal growth factor receptor 2 and displays special biological and clinicopathological characteristics. This subtype is aggressive in nature with high histological grade. Besides invasive ductal carcinoma,several special histological types have also been found. The features of the TNBC subgroup roughly parallel those of the basal-like subgroup. Due to the lack of molecular targets, this subgroup has no chance of endocrine treatment and target therapy. Currently, the treatment of TNBC is dominated by chemotherapy based on anthracycline with suboptimal efficacy. Overall, the prognosis has remained quite poor. Emerging evidence indicates that patients regimens with triple negative breast cancer usually displays high rate of early recurrence and distant metastasis. Both the diseasefree survival and overall survival rates are low. Although this subtype which shows same immunohistologic pattern, great heterogeneity still exists within the group causing distinctions in morphology, prognosis, and more importantly, drastically different reactions to same treatment protocol. In recent years, TNBC has been widely concerned by both clinician and pathologist. Several targeted drugs for corresponding signal pathway as well as the subtype of triple negative breast cancer have been widely studied. This article focused on the advances in clinicopathological characteristics, new subtypes and treatment of triple negative breast cancer.

Objective To investigate the risk factors of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt (TIPS) .Methods 181 cases of patients after TIPS diagnosed in this department from January 2009 to December 2013 were reviewed .The patients were divided into two groups:42 cases with hepatic encephalopathy (HE group)and 139 cases without he-patic encephalopathy(control group) .To evaluate the possible risk factors such as age ,sex ,pathogeny of cirrhosis ,complications [(Child-Pugh score ,portal pressure gradient (PPG ) ,Child-Pugh grading ,fibrinogen ,hemoglobin ,serum natrium ] were recorded and analyzed .Unconditional logistic regression model(W ald test)was used to screen the independent risk factors .Receiver operating characteristic curve was plotted and the area under it was calculated to evaluate the diagnose performance .Results There was no significant statistical difference between HE group and control group about age ,sex and pathogeny of cirrhosis(P>0 .05) .Analysis of unconditional logistic regression model indicated that Child-Pugh score ,PPG ,Child-Pugh grading ,were single risk factors of HE after operation(OR=1 .29 ,1 .06 ,1 .22) ,while fibrinogen ,hemoglobin and serum natrium were protective factors .ROC curve was plotted and the AUC was calculated to evaluate the diagnose performance ,which indicated the sequence was that ,Child-Pugh score> PPG>Child-Pugh grading>hemoglobin>fibrinogen>serum natrium .Conclusion Child-Pugh score ,PPG ,Child-Pugh grading , fibrinogen ,hemoglobin and serum natrium were single hazard factors of HE after operation .

[Objective] To search Zhang Taiyan’s prentice of medicine,study his effect of academic thought and academic method. [Method] Complete collection of periodicals in the Republic of China, with the research methods of philology, based on the academic history.[Result]Many famous disciples of Zhang Taiyan are from Jiangsu and Zhejiang,such as Lu Yuanlei,Xu Hengzhi,Zhang Cigong,Chen Cunren,Zhang Polang,Yu Yunxiu,Sun Shiyang. They inherited Zhang Taiyan’s effect of academic thought and academic method,such as widely collecting prescriptions and verifying,canoniz Changsha school,referring Chinese medicine in Japan,paying attention to the textual research.[Conclusion]Zhang Taiyan’s prentice of medicine made outstanding contributions to Chinese medicine. To search Zhang Taiyan and his prentice of medicine is helpful to study the medicine history of Wu and Yue area.

Circulating tumor cells (CTCs) play pivotal roles for monitoring the tumor metastasis and prognosis.The nanotechnology provides a favourable platform for CTCs detection,and enables CTCs to be more promising for practical application.Meanwhile,the nanoscale device by virtue of nanotechnology has broad application prospects in eliminating CTCs and offers a new direction in the field of anti-cancer.