The strategies for constructing pharmaceutical subject repository union in China and the development trend of pharmaceutical subject repository union were studied form the aspects of its cooperation models, platform building, resources acquisition and organization, access policy formation;copyright, quality control, and fund source.The cooperation models were divided into centralized storage model, distribution acquisition model and hierarchical construction model.Pharmaceutical subject repository union platform was built according to the different cooperation models.

Objective:To evaluate the emergency diagnosis of severe myocardial contusion. Methods:Thirty patients with serious blunt chest trauma underwent sonourethrography,all of whom underwent conventional myocardial markers simultaneously.All the observation time phases were set as 1 h,2 h,4 h and 8 h after injuries. Results:Both the concentrations of cardiac troponin T (cTn-T)and cardiac troponin (IcTn-I)at 8h after injuries were significantly higher than those at 1h after injuries(P

Objective To study chronic bronchitis rats oxidant/antioxidant related indicators Ho flavored Dingchuan Decoction.Methods 50 SD rats were adopted improved smoked method to make chronic bronchitis model.From the 20th day of the modeling,blank control group and model control group gavage were treated with saline.The positive control group were treated with Cough Mixture.The experimental groupwere dealed with Ho flavored Dingchuan Decoction high,medium and low-dose.20 day later,the cotent of SOD,CAT,MDA in rat serum,lung tissue and balf were observed.Results Compare to blank control group,the cotent of SOD[(188.11 ± 9.37)U/ml、(276.11 ±30.28) U/ml,(8.80 ± 1.49)U/ml],CAT[(9.40 ±2.04)U/ml,(26.57±4.26)U/ml,(1.58±0.26) U/ml] in rat serum,lung tissue of the experimental group significantly reduced (P ＜0.01) ; MDA [(5.64 ± 0.52) μmol/L,(6.32 ± 2.10) μmol/L,(28.08 ± 2.43) μ mol/L) increased (P ＜0.05 or P ＜ 0.01).Ho flavored Dingchuan Decoction can effectively reverse these changes,and showed a significant dose-dependent.The Ho flavored Dingchuantang,in middle and high-dose serum SOD [(253.05 ± 7.81) U/ml,(256.92 ± 5.37)U/ml],CAT [(14.86 ± 2.49)U/ml,(18.22 ± 2.65)U/ml] higher than those of blank control group.And the Ho flavored Dingchuantang,in middle and high-dose lung tissue SOD [(470.91 ± 11.46)U/mgprot,(482.91 ± 14.32)U/mgprot),CAT [(46.87 ± 3.75)U/mgprot,(55.49 ±3.33)U/mgprot] higher than those of blank control group.Content close to the normal level.Conclusion The Ho flavored Dingchuan Decoction has significantly reduce the oxidative stress in chronic bronchitis.

AIM: To investigate the clinical effect of compound anisodine on patients with paralytic strabismus and the influence on malondialdehyde ( MDA ) and superoxide dismutase ( SOD) .
●METHODS: Seventy cases diagnosed with paralytic strabismus from Jun. 2008 to Dec. 2014 were selected as treatment group; another 70 cases with paralytic strabismus from Oct. 2000 to Jan. 2008 were chosen as control group. The control group was given with conventional therapy, and treatment group was additionally injected with compound anisodine subcutaneously on the basis of the control group. Treatment lasted for two courses about 28days. The effect of compound anisodine on patients with paralytic strabismus was evaluated and the serum level of MDA and SOD was also measured before and after treatment.
●RESULTS: ln treatment group, 54 cases were cured (77%), 14 cases improved (20%) and 2 cases had no response ( 3%) and total efficiency reached to 97%. ln control group, 32 cases were cured ( 46%) , 21 cases improved ( 30%) and 17 cases had no response ( 24%) and total efficiency was 76%. The cure rate and total efficiency of treatment group were significantly higher than that of control group (P<0. 05). Before treatment, the level of SOD and MDA was equal in two groups. After treatment, increased SOD level and decreased MDA level was observed in both groups. However, compound anisodine were significantly increased SOD level and reduced MDA level when compared with conventional therapy (P<0. 05).
●CONCLUSION: The compound anisodine shows the beneficial effect on patients with paralytic strabismus. Compound anisodine may exert the effect via increasing SOD and reducing MDA.

Research on the application of ocular response analyzer has become one of the main issues in ophthalmology.The ocular response analyzer has been used in the measurement of ocular bioparameters of glaucoma,cataract,heretability and refractive surgery.As a newly invented non-contact tonometric device,the ocular response analyzer is helpful not only in the measurement of intraocular pressure (IOP),but also in the determination and calculation of two corneal biomechanical properties:corneal hysteresis (CH) and corneal resistance factor (CRF).It offers the possibility of measuring corneal biomechanical properties in vivo.This article primarily reviewed the application of the ocular response analyzer in terms of pre-operative examination and post-operative follow-up in excimer laser surgery.

The short stature homeobox-containing(SHOX) gene,located in the short-arm pseudoautosomal region (PAR1) of the sex chromosomes,is one of the recently discovered genes,which is related to short stature.Its encoded protein,as a transcription activator,plays an important role in the regulation of growth.It has now been confirmed that the human SHOX gene mutation can cause L?ri-Weill syndrome,Turner syndrome,idiopathic short stature growth and its related characteristic skeletal deformities.This review makes a summary about SHOX gene defects,its clinical phenotype and treatment.

Network management of health record is an important measure to realize the aim of equalization of the basic public health services.Nowadays,a lot of ethical hidden dangers have been left when most areas of our country doing the job of network management of health record,such as,violating the citizen rights of privacy and informed consent,impairing citizen rights of autonomy and health,lacking of balance mechanism between the individual interest and public benefit,and so on.In this paper,these phenomena are considered based on ethical rules,and suggestions centred around the improving of trust valuations between doctors and patients,information technology,medical moral education and the setting up of related laws for improvement have been provided.

To evaluate the value of detection of DNA aneuploidy in exfoliated airway epithelia cells of sputum specimens by the automated image cytometry for the identification of lung cancer, 100 patients were divided into patient group (50 patients with lung cancer) and control group (30 patients with tuberculosis and 20 healthy people). Sputum was obtained for the quantitative analysis of DNA content of exfoliated airway epithelial cells with the automated image cytometry, together with the examinations of brush cytology and conventional sputum cytology. Our results showed that DNA aneuploidy (DI>2.5 or 5c) was found in 20 out of 50 sputum samples of lung cancer, 1 out of 30 sputum samples from tuberculosis patients, and none of 20 sputum samples from healthy people. The positive rates of conventional sputum cytology and brush cytology were 16% and 32%, which was lower than that of DNA aneuploidy detection by the automated image cytometry (P<0.01, P>0.05). Our study showed-that automated image cytometry, which uses DNA aneuploidy as a marker for tumor, can detect the malignant cells in sputum samples of lung cancer and it is a sensitive and specific method serving as a complement for the diagnosis of lung cancer.
*Aneuploidy ; DNA, Neoplasm/*analysis ; Image Cytometry/methods ; Image Processing, Computer-Assisted ; Lung Neoplasms/genetics ; Lung Neoplasms/*pathology ; Sensitivity and Specificity ; Sputum/*cytology

Objective To explore the potential benefits of liver resection for patients with liver metastasis of pancreatic neuroendocrine tumors(PNETs).Methods We searched CNKI,CBM,MEDLINE,The Cochrane Library,EMBASE until Aug.2012 for identifying randomized trials.Randomized clinical trials(irrespective of language,blinding,or publication status)were collected to compare liver resection alone versus other unresected interventions(radiofrequency ablation,chemotherapy,hepatic arterial embolization,or drugs et al)in patients with liver metastasis from PNETs.Two authors independently identified trials for inclusion.The primary outcome was survival,treatment-related mortality(30-day mortality)and relief of symptoms.RevMan 5.1 software was used for Meta analysis.Results There was no meta-anlaysis,RCT,QRCT showing the clinical effect of operation for the treatment of pancreatic neuroendocrine tumor with liver metastasis.Only 6 cohort studies found were eligible and analyzed,involving 1020 patients.Compared with unresected treatment,the 3-year survival rate[OR =0.24,95％ CI (0.11,0.53),P =0.0004],the 5-year survival rate [OR =0.16,95 ％ CI (0.12,0.22),P ＜ 0.00001] and median survival time (109.5 ± 19.02 vs 31.2 ± 5.97 months,P ＜ 0.01) were significantly increased in liver resection alone.The symptom relief rate was substantially higher in the surgical group than in the non-surgical group(98.3 ±2.8 vs 62.5 ± 22.0,P ＜ 0.05).Perioperative mortality rate was lower in surgical group.Conclusions Surgical resection is the preferred treatment of PNETs in patients with liver metastasis.Surgery is safe and effective.Survival time is significantly extended and symptom relief rate significantly improves.Due to lack of randomized controlled trial (RCT),surgical resection for treatment of liver metastasis of PNETs requires further more randomized control study.