Autism in children is a complex disease of developmental disorder in nervous system,characterized by social deficits,impaired language and communication,stereotyped and repetitive behaviors.Studies indicate oxidative stress is associated with autism.Because of genic and environmental factors,children with autism are susceptible to oxidative stress.Oxidative stress induce the development of autism through abnormal metabolism of the membrane lipid,inflammation and excitotoxicity,autoimmunity,energy metabolism disorder,et al.Anti-oxidation might be a safe and effective therapy for autism.

Objective To compare and analyze two methods for determination of vaccine particle content in freeze-dried human rabies vaccine(Vero cells)bulk and final bulk,and to provide experimental basis for establishing standard detection method for particle content and purity of rabies vaccine products.Methods The samples were subjected to vaccine particle cracking,BCA quantification,and size exclusion chromatograghy-high performance liquid chromatography(SEC-HPLC)analysis. BCA-SEC relative area method and BCA-SEC vaccine particle standard curve method were used to detect the total protein concentration of the bulk and final bulk,separately,and the content of vaccine particles was quantitatively analy-zed. Finally,the detection results of the two methods were compared and analyzed.Results The content of vaccine particles in freeze-dried human rabies vaccine bulk was determined to be within 256-305 μg/mL by the two methods,with an average value of 267-285 μg/mL,and the relative standard deviations(RSDs)ranged from 5. 8% to 10. 2%,with good consistency between two methods. The content of vaccine particles in freeze-dried human rabies vaccine final bulk was determined to be in the range of 149-169 μg/mL by the two methods,with an average value of 152-164 μg/mL,and the two methods showed the RSDs between 0. 9%-4. 7% with good consistency.Conclusion The measured value by BCA-SEC standard curve method deviates less from the expected value and is closer to the actual situation of samples,so it is recommended to use this method as a reference for enterprises.

Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods：The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1，3，5，7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results： There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.

In recent years,with the improvement of the genetic inspection techniques,some of the epileptic en-cephalopathy whose causes were unknown are associated with genetic factor.The result shows that one kind of epileptic encephalopathy may be associated with a variety of genetic mutations or copy number variation (CNV),one gene muta-tion or CNV also may lead to a lot of epileptic encephalopathy.In addition,the new clinical features and genetic poly-morphism were discovered increased.So that epileptic encephalopathy which named from the disease -causing gene was also increased.This article will summarize the clinical phenotype characteristics,genetic studies and future of the newly discovered CHD2 myoclonus encephalopathy.

Vitamin B6 related epilepsy is a group of epileptic diseases,seizures in which could not be con-trolled by antiepileptic drugs,but could be controlled or obviously improved by vitamin B6 .It comprises of pyridoxine dependent epilepsy and pyridoxine responsive epilepsy predominantly,and the latter includes vitamin B6 responsive in-fantile spasms,pyridox(am)ine -5′-phosphate oxidase (PNPO)deficiency,hyperphosphatasia mental retardation syndrome (Mabry syndrome)and so on.The clinical presentations of the diseases above are nonspecific,manifesting as refractory seizures onset in neonatal or infantile period,which need to be distinguished from other diseases such as new-born hypoxic ischemic encephalopathy,Ohtahara syndrome and non -ketotic hyperglycinemia.Pyridoxine dependent epilepsy,PNPO deficiency and Mabry syndrome have relative specific biomarkers and known disease -causing genes, which are helpful for diagnosis.It is suggested that pyridoxine or pyridoxal phosphate should be tried first for all patients started seizures early (including all infantile spasms patients),avoiding missing these diseases.And once diagnosed,vi-tamin B6 should be maintained long -term or all the life according to the detailed disease.

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare disorder of hematopoietic stem cells due to acquisition of somatic mutations.Somatic mutations in phosphatidylinositol glycan class A (PIGA) account for intravascular hemolysis and other PNH manifestations,but the pathophysiology of clonal expansion of PNH cells cannot be elucidated clearly.PNH is closely related to aplastic anemia and myelodysplastic syndromes.Today,the gold standard for PNH is flow cytometry to detect the absence or severe deficiency of glycosylphosphatidylinositol (GPI)-anchored proteins on white and red blood cells.However,PNH diagnosed by phenotype is a group of heterogeneous disease in pathogenesis.Eculizumab,a first-in-class monoclonal antibody that inhibits terminal complement,is highly effective in stopping intravascular hemolysis and improving quality of life.Further research on the pathogenesis of PNH would be helpful to understand the underlying reasons for PNH phenotype cells in different patients,improve differential diagnosis and more targeted and specific therapy.Research progress in recent years will be reviewed in this article.

The "opening-closing-pivoting" theory on the six meridians is one of the difficult points in the study of Shang Han Lun (Treatise on Cold Diseases). The authors give an explanation on the space and time differentiation of three-yin and three-yang as well as the "opening-closing-pivoting" theory based on the predecessor's experience, and then analyze the physiological and pathological characteristics of the six-meridian disorders.

Objective To investigate the current situation of healthcare-associated infection (HAI)management in basic level private medical institutions.Methods 118 basic level private medical institutions in a district of Chengdu were investigated through visiting and questionnaire.Results Of 118 medical institutions,only 2 had HAI manage-ment personnel,4 had HAI management system.22.03% had hand washing facilities in therapeutic rooms and con-sulting rooms,37.29% of institutions stored aseptic items in accordance with the requirements,55.93% of institu-tions disposed medical waste at disposal center;99.15% handled reusable items by users.Conclusion HAI manage-ment status is worried,health administrative department should strengthen support and supervision,especially in-tensified the supervision of private dental clinics.

Objective To investigate the clinical efficacy and safety of topical corticosteroids as adjunctive plan in the therapy of bacterial corneal ulcers (BCU).Methods 62 eligible patients with confirmed BCU were screened and divided into 2 groups randomly.Both groups were treated with topical tobramycin for 3 weeks,the observation group received topical tobramycin for at least 48 hours and then treated with 0.02％ fluorometholone for 3 weeks,and decrement setp by step.Best spectacle corrected visual acuity(BSCVA),fluctuation of intraocular pressure (IOP) before and after therapy,time to corneal reepithelialization and clarity,severe adverse event were observed.Results After 3 weeks,BSCVA of both groups increased compared to their baselines,however,no significant difference between the two groups.Patients whose IOP exceed 21mm Hg in the control group was statistically more than that in the observation group(x2 =7.272,P ＜ 0.05).Follow up for 3 months,increasing of BSCVA in the observationgroup was significantly higher than the control group(t =2.388,P ＜ 0.05) ;and lOP of both two groups almost recuperated to baselines.Although the time to corneal reepithelialization of the control group (11.3 ± 4.5) d was shorter than observation group [(14.7 ± 5.2) d] (t =2.707,P ＜ 0.05),the recovery of corneal clarity in observation group was superior to control group (x2 =8.207,P ＜ 0.05).In addition,no severe adverse events were observed during this period.Conclusion Although prolong the healing time of corneal epithelium,corticosteroids as an adjunctive plan in the therapy of BCU may reduce immune-mediated damage,decrease scarring,control IOP fluctuation and improve BSCVA,but with no safety concerns.

?AIM: To investigate the corneal high order aberrations in adults with myopia and to study its correlations with myopic degree and keratometry.?METHODS: A total of 63 patients ( 126 eyes ) from the ophthalmology department of our hospital were included in this study during May 2014 to June 2015. Visual acuity, intraocular pressure ( IOP ) , diopter, slit lamp examination, central corneal thickness ( CCT ) , funduscopic examination, corneal topography and corneal total higher order aberrations ( THOAs ) were recorded. Right eye was selected. According to the spherical equivalence ( SE) , 63 eyes were divided into two groups, the mild to moderate myopia group (M-M group, SE<-6. 00D) and the high myopia group (H group, SE≥-6. 00D). The correlations of corneal THOAs with diopter, keratometry, IOP and CCT were analyzed. Kolmogorov-Smirnov Test was used for normality test. Independent-samples t test was used for comparison between two groups. Pearson correlation analysis was for comparison between two continuous variables.?RESULTS: Z44 was found a statistic difference between the M-M group and H group (t=2. 51,P=0. 02). IOP was negatively correlated with Z5-3(r= -0. 52,P=0. 01), and CCT was positively correlated with Z4-4(r=0. 43,P=0. 03) in H group. There was no correlation between higher order aberrations and SE in M-M group ( all P>0. 05 ) Total coma aberration ( TCA ) was negatively correlated with SE (r=-0. 57,P=0. 002) and spherical degree (Sph, r=-0. 55,P=0. 003) in H group. Z40 was positively correlated with average K (AK) (M-M group:r=0. 40,P=0. 02. H group:r=0. 55, P=0. 03), K steep (Ks) (M-M group:r=0.39, P=0.02. H group:r=0.51, P=0.01) and K flat (Kf) ( M-M group:r=0. 40, P=0. 02. H group:r=0. 57, P=0. 01) in both groups. Z53was negatively correlated with Ks(r=-0.4, P=0. 04) in H group.?CONCLUSION: The THOAs parameters are influenced by diopter and keratometry. TCA is negatively correlated with SE and Sph in patients with high myopia, and spherical aberration is positively correlated with keratometry.