Objective To investigate the MRI characteristics of the tumor of optic pathway and damage degree of visual function. Methods Analysis of 119 cases surgically and pathologically proved tumor suffering from optic pathway was carried out retrospectively. The patients included:36 ones with tumors in anterior segment of optic pathway,70 ones in middle segment of optic pathway,and 13 ones in posterior segment of optic pathway. The MRI examination series were transverse T 1WI SE,transverse and coronal T 2WI TSE,coronal T 2WI SPIR,and transverse,sagittal,coronal T 1WI SE after Gd DTPA enhancement. Results The tumors of optic pathway included:the primary tumors of the optic pathway and the tumors of any other part of the body which invaded the optic pathway. There was a special MRI feature on the each tumor suffering from optic pathway,but the pituitary adenoma which affects visual function was the most common tumor. Conclusion MRI is an effective method in the diagnosis of optic pathway tumor.

Objective To evaluate suprapubic transvesical prostatectomy without partition of the prostatic cavity. Methods From Nov.1999 to Mar.2000,20 cases of BPH were treated by suprapubic transvesical prostatectomy without partition of the prostatic cavity and the therapeutic result was evaluated. Results The blood loss has been less.The patients have been followed up for 1～5 months.Neither incontinence nor bladder outlet obstruction has been noted.IPSS decreased from 29.4?2.0 to 8.8?2.0,Qmax increased from 6.8?1.3 ml/s to 16.8?2.0 ml/s,AFR increased from 3.5?0.9 ml/s to 10.0? 2.0 ml/s and QOL decreased from 5.5?0.5 to 1.7?0.5.All these differences were statistically significant ( P

Objective To evaluate wedge resection of the back labium renalis and intrasinusal pyelolithotomy for staghorn calculi of kidney in patients with intrarenal pelvis and renal malrotation. Methods From August 1998 to February 2001, 18 cases of staghorn stones of kidney were removed via the wedge resection of the back labium of hilum renalis and incision of the intrarenal sinus. Results The mean operative time was 110 min and the mean blood loss 300 ml.The biggest stone was 6.5 cm?5.0 cm?3.0 cm and in one case the number of stones amounted to 1 400. Postoperative KUB showed no residual stones and postoperative IVU indicated normal renal function.All patients have been followed up for 1～3 years and have been free of stone. Conclusions The procedure has the advantages of no need of pedicle renalis occlusion,less bleeding,a clear operation field,easy staghorn calculi removal and the avoidance of renal hilum outlet obstruction.

ObjectiveTo evaluate wedge resection of th e back labium renalis in the repairation of iatrogenic pelvis tear. MethodsFrom October 1999 to February 2002,7 cases of iatrogenic pel vis tear were repaired with wedge resection of the back labium of renal hilum.Th ere were 5 male patients and 2 female with an average age of 45.The tear occurre d during lithotomy for pelvis stone in 6 and transureteroscopic lithotripsy in 1.ResultsThe mean operative time was 130 min and the m ean blood loss 300 ml,postoperative IVU showed no obstruction of the renal pelvi s,and the renal function has been normal.All patients have been followed up for 3 months to 3 years.Neither hydronephrosis nor renal pelvis obstruction has been noted.ConclusionsThe procedure has the advantages of no need of predicle occlusion,less bleeding,a clear operation field,easy repaira tion,and the avoidance of pelvis obstruction.

Objective To observe the features of the manifestations of fundus fluorescein angiography (FFA) in multiple sclerosis (MS) and their value in clinical diagnosis. Methods The clinical data of 42 patients (84 eyes) with MS diagnosed by magnetic resonance imaging (MRI) and examination of cerebrospinal fluid (CSF) were retrospectively analyzed. The clinical data included visual acuity, ocular fundus examined by direct ophthalmoscope after mydriasis, FFA, visual field, CSF,visual evoked potential (VEP) and MRI examination. Results In 42 patients (84 eyes),the positive detectable rate of examination of direct ophthalmoscope, CSF, visual field, VEP, and MRI was 36.9%, 21.4%, (71.4%, and) 83.3% respectively. Abnormal results of FFA were found in 44 eyes (52.38%), including papillitis in 4 eyes (4.76%)at the early stage with extended physiological scotoma and central scotoma; neuroretinitis in 7 eyes (8.33%)at the medium stage with central or para- central scotoma; optic atrophy in 33 eyes(39.29%) at the late stage with centripetal constriction and even tubular visual field. Conclusion The main angiographic features of MS are papillitis, neuroretinitis and optic atrophy. The manifestations of FFA combined with the results of examination of CSF,visual field, VEP and MRI is helpful for comprehensive and exact diagnosis of MS.

Objective To establish the model of abdominal heterotopic heart transplantation and grope steady operation methods.Methods The heart transplantation was performed in the control group(group A) and experiment group(group B) with 30 rats in each group.Another 30 animals not performed the operation were as the blank group(group C).During the procurement of the donor heart saline solution containing heparitin was injected into the inferior vena cava of liver.At the same time abdominal aorta was transected.Then heart was perfused until it stopped.The activity of superoxide dismutase(SOD) and content of malondialdehyde(MDA) in blood was measured after transplantation.Results The total perfusion time of the graft was shortened within 2 min and hot ischemia time was shortened within 25 s in the group B.The activity of SOD and content of MDA between two groups was significantly different(P<0.05).Conclusion The perfusing method improved with new technique is quicker and more effective than the traditional one and results in the successful rate improvement.

Objectives To compare the co-infection statues of HSV, HBV and HCV in patients with STD and HIV infection for providing evidence of developing prevention and control strategies. Methods Serum samples confirmed to be infected with HIV/AIDS by Western blot, and serum samples of patients with STDs (syphilis, gonorrhoea and chlamydial infection) were tested for HSV2-IgG, HSV2-IgM, HBsAg and HCV-IgG by ELISA. The detection levels were compared between the two groups. Results Out of 76 specimens in STD group, HSV2-IgG was detected in 24 specimens (31.58%), HSV2-IgM in one specimen (1.32%), HBsAg positive in 8 (10.53%), and HCV antibody positive in 4 (1.32%). In 14 specimens of HIV/AIDS group, HSV2-IgG were detected in 7 (50.00%); HSV2-IgM in 5 (35.71%);8 (578.14%) were positive for HBsAg and 3 (21.43%) for HCV. In a total of 90 specimens, both HSV and HBV were detected in 6 specimens, both HSV-IgM and HBV in 2, and the four above-mentioned antibodies in 2. The infection rates of HSV, HBV and HCV were significantly higher in HIV-infected specimens than those in the STD specimens (P

BACKGROUND: Rejection is the main cause of the failure in small intestine transplantation. Cellular immunity mediated by chemotatic factor and the receptor plays an important role in acute rejection. We regard chemokine receptor as target site to design the treatment, which may provide reference for the immunotherapy in clinical small intestine transplantation. OBJECTIVE: To observe the effect of chemokine receptor antagonist, regulated upon activation, normal T cell expressed and secreted (Met-RANTES), on the survival time and histopathological changes of allograft rats which have received heterotopic small intestine transplantation, and the coordinative effects of Met-RANTES used together with low-dose tacrolimus.DESIGN, TIME AND SETTING: Randomized complete-block design and controlled animal experiment, performed in the Department of Gastrointestinal Surgery, Xijing Hospital, the Fourth Military Medical University of Chinese PLA between September 2003 and March 2005.MATERIALS: 180 healthy adult male rats including 90 rats (donors) and 90 Wistar rats (recipients) were involved in this study. Heterotopic segmental small intestine transplantation was performed.METHODS: The rats were randomly divided into 3 groups with 30 rats for each group. Control group: Rats were treated with heterotopic small intestine transplantation alone; Met-RANTES group: Rats were treated with an intraperitoneal injection of Met-RANTES (200 μg/d) at 0-7 days after transplantation; Met-RANTES+low-dose FK506 group: Rats were treated with an intraperitoneal injection of Met-RANTES (200 μg/d)+tacrolimus (0.5 mg/kg/d) at 0-7 days after transplantation.MAIN OUTCOME MEASURES: Gross status and survival time were detected; in addition, every 6 rats were sacrificed at different time points, such as 1,3, 5, and 7 days after transplantation, to compare histopathological changes. RESULTS: Following transplantation, 90 Wistar rats (recipients) were all involved in the final analysis. The survival time median in the control group was 7.2 days (1.5), and all rats died of acute rejection and infection. Histopathological examination showed that mild, moderate and severe rejections were detected at day 3, 5, and 7 after transplantation, respectively. The survival time median in the Met-RANTES group was 19.2 days (16.4), which was significantly longer than the control group (P<0.01). The survival time median in the Met-RANTES+low-dose tacrolimus group was 30.9 days (9.0), and there were significant differences in survival rate as compared with control group and Met-RANTES group (P<0.01). While the rats in the Met-RANTES group and the Met-RANTES+low-dose tacrolimus group showed no obvious indication of rejection.CONCLUSION: Met-RANTES may obviously inhibit acute rejection following small intestine transplantation, effectively protect the function of grafts, and significantly prolong the survival time of the recipients. In addition, Met-RANTES may enhance the immunosuppressive function of low-dose tacrolimus.

Objective To investigate the expression of angiopoietin 2 gene in glioma and its relationship with angiogenesis.Methods The expression of angiopoietin 2 were examined by RT-PCR in 52 cases of glioma and 8 normal brain tissues.The vascular development was measured by microvessel count(MVC) which was immunostained with anti-CD34 monoclonal antibody.Results The expression of ang2 mRNA was found in 50 cases of gliomas and 8 normal brain tissues,and the expression of ang2 mRNA was significantly positively correlated with MVC(r=0.821,P

AIM:To explore the effect of carbon monoxide (CO) on hypoxic proliferation of rat pulmonary arterial smooth muscle cell(PASMC). METHODS:Rats were randomly divided into four groups, including: (1)N:Normoxia group (21% O 2); (2)H:Hypoxia group (1% O 2); (3)CO+H:CO+hypoxia group (3% CO+1% O 2),tested by immunocytochemical analysis,[3H]-TdR incorporation and flow cytometry (FCM). RESULTS: (1)Flow cytometric DNA analysis revealed that hypoxia induced significantly enhancement of G 2/M phase and decreased G 0/G 1 phase of PASMC ( P