With the frequent culture communication between china and Japan, YanQi theory of TCM spread to Japan and exerted an influence on Japanese medicine. From the volume of"YunQi" in "The literature investigation of TCM", we can tell YunQi theory occupied a major position in the Japanese medical literatures which spread from china. The primary books of YunQi edited by Japanese scholars reveal Japanese doctors are apt to use this theory to promote their clinical practice. The accordant cognition of Chinese and Japanese scholars is that Japanese doctors accept the value of YunQi theory and beware of it's deficiency in clinic.

Objective To investigate the changes in small-diameter sensory neuron (SNS)/ peripheral nerve type 3(PN3) Na+ channel transcript and in tetrodotoxin-resistant (TTX-R) Na+ current in dorsal root ganglion (DRG) neurons in a chronic constriction injury (CCI) model of neuropathic pain. Methods Eighteen rats were divided into 6 groups of 3 animals each. Chronic constriction injury model was established after Dib-Hajj et al. Pain threshold was significantly lowered after CCI as compared with that in control group. The animals were deeply anesthetized and rapidly decapitated 14 days after surgery. The L4-5 DRG of the operated side was removed and crushed and total RNA was extracted with trizol reagent. The DRG of the contralateral side was used as control. The change in SNS/PN3 Na + channel expression was determined by semi-reverse transcriptase-PCR. The DRG neurons were isolated enzymatically and the change in voltage-gated TTX-R Na+ current was recorded using whole-cell patch clamp technique. Results Sensory neuron specific TTX-R Na+ channel transcript SNS/PN3 was down-regulated by 60% 14 days after CCI as compared with that in control group. TTX-R Na+ current density was significantly reduced but its activation and steady state inactivation were unchanged. Conclusions Na+ channel SNS/PN3 is involved in the hyperextability of the primary sensory neurons after CCI.

BACKGROUND:One-lung ventilation is wildly applied in cardiothoracic surgery including general surgery and lung transplantation surgery.There have been much more researches of one-lung ventilation in recent years.OBJECTIVE:To analyze and conclude the research progress of one-lung ventilation with a view to improve the effectiveness of one-lung ventilation and reduce complications during and after one-lung ventilation.RETRIEVAL STRATEGY:A computer-based search of Medline was undertaken to identify the articles about one-lung ventilation published from January 1980 to October 2007 with the keywords "one-lung ventilation,hypoxemia,lung transplantation",and the language was limited to English.Chinese articles about one-lung ventilation punished from January 2000 to October 2007 were searched in China National Knowledge Infrastructure(CNKI),with the keywords "one-lung ventilation,hypoxemia,lung transplantation" and the language was limited to Chinese.The materials were selected firstly and full texts were searched.Inclusion criteria:①the basic and clinical study on one-lung ventilation;②the clinical study on anesthesia of lung transplantation.Exclusion criteria:①repetitive study;②reviews.Over 300 articles on one-lung ventilation were collected,in which 28 were accordant with inclusion criteria.LITERATURE EVALUATION:Of the 28 articles,15 were about the study of the management during one-lung ventilation,and 13 were the hypoxemia study of one-lung ventilation.DATA SYNTHESIS:After the selected literatures were analyzed and arranged,the management of one-lung ventilation including the selection of ventilation mode,the settings of tidal volume and respiratory frequency,fraction of inspired oxygen,fluid management was analyzed.Hypoxemia is the biggest challenge during one-lung ventilation.We may improve arterial oxygenation and reduce the occurrence of hypoxemia by various ways.However,the management of one-lung ventilation is still controversial and needs further study.CONCLUSION:With the study on the management of one-lung ventilation and the handling of common matters deepens continuously,the application of one-lung ventilation will be more widespread and more safe.

Amino Acid Sequence ; Animals ; Birds ; Cell Transformation, Viral ; Herpesvirus 2, Gallid ; genetics ; physiology ; Marek Disease ; virology ; Models, Biological ; Molecular Sequence Data ; Oncogene Proteins, Viral ; genetics ; physiology

Dry age-related macular degeneration(AMD)is a prevalent form of irreversible blindness worldwide in the elderly population with an increasing incidence in China.It is found that some dry AMD will develop to wet AMD and therefore damage visual function.Pathogenesis of AMD is below understand now,but it is thought to be related to the light-induced ocular damage,oxidative stress response,extracellular disposition and immunology.Up to now,effective and safe prevention and treatment methods of AMD are still studying,especially wet AMD,but the concern on dry AMD is not enough.Some basic and clinical pharmaceutics researches on AMD include antioxidants,anti-extracellular disposition drugs and anti-inflammatory agents.With the rise of aging population,we should pay special attention to the research on pathogenesis,diagnosis and treatment of dry AMD.

Objective To analyse the clinicopathologic factors affecting prognosis and fertility of patients with malignant ovarian germ cell tumor (MOGCT).Methods The medical records and follow up data of 106 patients with MOGCT treated at Affiliated Tumor Hospital of Guangxi Medical University between January 1986 and December 2010.Kaplan-Meier method was used to analyse survival curves.The different prognoses between different clinicopathologic factor was evaluated by univariate analysis and log-rank test.The multivariate analysis was performed by the Cox proportional hazard regression method.Logistic regression analysis was used to evaluate the influence of different factors on the prognoses and fertility.Results The median age at primary treatment was 22 years old (range:9-61 years old),59 patients received fertility-preserving surgery,45 patients received radical surgery,only 2 cases performed biopsy; 94 patients received postoperative adjuvant chemotherapy.Median follow-up time was 56.5 months (range:2-309 months),there were 11 cases recurrences,23 cases died from cancer.Of 47 patients live without tumor,45 patients had normal menstrual.Of the 39 patients desiring pregnancy,31 cases got 33 successful pregnancies,resulting in 33 live births.There is no statistically significant difference (P ＞0.05) in progression free survival (PFS ; 67.6％ versus 63.3 ％) and overall survival (OS ; 70.0％ versus 64.1％) and mortality [15％ (9/59) versus 31％ (14/45)] between fertility-preserving surgery patients and radical surgery patients.The univariate analysis showed that the pathological types,postoperative residual tumor size,lymph nodes and omental resection were associated with OS (P ＜ 0.1),and postoperative residual tumor size,chemotherapy cycles,lymph nodes and omental resection were associated with PFS (P ＜0.1).The multivariate analysis showed only the postoperative residual tumor size was independent prognostic factor of OS (P =0.039),and postoperative residual tumor size,chemotherapy cycles,lymph nodes resection were independent prognostic factors of PFS (P ＜ 0.05).There is no statistically significant difference in OS,PFS and fertility between fertility-preserving surgery patients treated with or without a comprehensive staging surgery (P ＞ 0.05).Conclusions MOGCT can achieve a good prognosis after surgery combined chemotherapy.Postoperative residual tumor size is independent prognostic factor of PFS and OS.Comprehensive staging surgery could not improve prognosis.Fertility-preserving surgery plus adjuvant chemotherapy appeared to have little or no effect on prognosis and fertility.

Objective To analyze the difference of the delivery date reckoned by traditional and modified formula for calculating the expected date of confinement (EDC).Methods The data of 2055 women (37-41+6 week) were collected who gave monotocousa term spontaneous birth in the Chongqing Health Center for Women and Children from Jan.2014 to Feb.2015.Of which 1300 were primipara,and 755 were multipara;and the data of 1224 women (39-41week) were collected,of which 832 were primipara,and 392 were multipara.The expected date was calculated with traditional and modified calculating formula,and then the actual delivery date was used for comparison and statistical analysis.Results The coincidence of actual delivery date with the estimated due date reckoned by traditional formula (39-41week) was 8.4％ in primipara and 9.7％ in multipara,and the coincidence reckoned by modified formula was 11.9％ in primipara and 14.8％ in multipara.The EDC estimated by modified formula was more precise than that calculated by traditional formula (P＜0.05).Conclusion The EDC calculated with modified formula is more accurate than that calculated with traditional formula.

Objective To seek the specific receptors associated with hepatitis B virus (HBV) adhesion by separating the binding protein of the HBV preS1 region in HepG2 and performing the mass spectrometry .Methods The immunomagnetic bead method was adopted to separate HepG2 membrane protein combined with preS1 peptide fragment and the binding protein was separated by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ,then the destination strips was analyzed by LC-MS/MS mass spectrometry and retrieved by the database .Results 16 bands were separated from HepG2 membrane proteins combined with preS1 by SDS-PAGE ;14 kinds of proteins were identified from 6 bands with better repeatability separated from HepG 2 membrane proteins combined with preS1 .Conclusion Protein analyzed by the mass spectrometry is mainly related with the material transport , cellular signal transduction ,antigen presentation ,immune regulation and energy metabolism .

Objective To explore the changes of cytokine levels and their roles in morbidity of autoimmune thyroid disease(AITD) in children.Methods The serum concentrations of interleukin-4(IL-4),interleukin-6(IL-6),tumor necrosis factor-?(TNF-?),interferon-? (IFN-?) were determined with enzyme linked immunosorbent assay (ELISA) among 30 patients with Graves′ disease (GD) and 20 patients with Hashimotos thyroiditis(HT) and 30 children without AITD subjects.Results The serum levels of IL-4 and IL-6 in patients with GD were higher than those of subjects (Pa

Objective To investigate the effects of 1% ropivacaine injected epineurally or intraneurally on the recovery of sciatic nerve from acute injury in rats. Methods Seventy-two healthy male Wistar rats weighing 220-250 g were xandomly assigned into 4 groups ( n = 18 each): group Ⅰ epineural injection of normal saline(NS)(group C1); group Ⅱ intraneural injection of NS (group C2); group Ⅲ epineural injection of 1% ropivacaine (group Epi-R) and group Ⅳ intraneural injection of 1% ropivacaine (group Intra-R). The animals were anesthetized with intraperitoneal 3% pentobarbital 60 mg/kg. The sciatic nerve was exposed and crushed with blood vessel clamp for 2 min. NS or 1% ropivacaine 0.2 ml was injected epineurally or intraneurally after release of the clamp.Sciatic nerve function was measured and sciatic nerve function index (SFI) was calculated at day 1, 3, 7, 14, 21and 28 after operation. Six animals in each group were anesthetized on the 14th and 28th day after operation and the nerve conduction velocity (NCV) of the sciatic nerve was measured. The sciatic nerve was then removed for histologic examination. Results There was no significant difference in SFI and NCV at all time points among group C1 , C2 and Epi-R. SFI was almost normal on the 28th day after operation in the 3 groups. The NCV was significantly slower at day 14 and 28 after operation in intra-R group than in the other 3 groups. Conclusion Intra-neural injection of ropivacaine can significantly delay the recovery of sciatic nerve from acute injury.