Taking Cancer Hospital Affiliated to Southwest University as an example,the paper states the deployment of m-health network,and puts forward several wireless network optimization schemes such as wireless environment optimization,channel capacity improvement,code efficiency increase and user access restriction through clinical business traffic monitoring and data analysis.Through the result,this scheme can improve the performance of the wireless network effectively.

Objective To investigate on blood group serology of crossmatching incompatibility of blood donors in clinical hospi‐tals in Nanchong from 2010 to 2014 .Methods According to blood group serological test ,direct antiglobulin test (DAT ) was used for blood specimen with crossmatching incompatibility where donor was suspected as the causes ,at the same time irregular antibody was screened and identified .Results Among 77 returned blood specimens ,positive DAT were found in 43 cases ;while irregular an‐tibodies were found in 18 cases ,with anti‐M and anti‐E as the major ,followed by anti‐Lea and anti‐P1;and 16 cases did not find ab‐normality .Conclusion Causes of crossmatching incompatibility of blood donors in clinical hospitals in Nanchong are mastered through the survey ,to improve the safety level of clinical blood transfusion and avoid the waste of the blood .

There are some issues with the payment mechanism in the hospital at present.For instant, lacking of the government subsidy,collecting money depending on drug and the price is unreasonable.All of these issues restrict the development of health care system,and influence the health care delivery and the cost control.The article suggests the following working must be well done:to strengthen the regionol plan- ning in order to prefer the rural area;defining the accountability.of the government for health care;dividing treatment and selling drug into two groups;reforming the price of health care service.

There has been no consensus on the efficacy of extracranial-intracranial arterial bypass (EIAB) for ischemic cerebrovascular disease. As an important factor in the pathophysiologic mechanisms of ischemic cerebrovascular disease, the changes of cerebral hemodynamics have received attention. The article expounds the clinical significance of EIAB from the point of view of cerebral hemodynamics, particularly presents its preoperative assessment, operative procedures, and efficacy criterion.

The activity of the negative regulator of cell cycle, P27, affects the normal process of cell cycle. As a type of cell cycle diseases, tumor is closely correlated with the activation of P27. It is shown that the level of P27 decreases in most kinds of tumors.By its influence on cell proliferation and apoptosis, P27 plays an important role in the occurrence and development of tumor. The activity of P27 is regulated by several pathways either at or after transcription, so various ideals which directly change the protein level of P27 or indirectly interfere the activities of elements in regulative pathways of P27 will provide many novel and efficient ways for therapies in tumors.

Top-quality courses are a pace-setter and a symbolic banner in the construction of the academic courses, and will have an inestimable role in the promotion of the higher education in China. The purpose to conduct top-quality course selection is to help the universities to establish and form the mechanism of course construction and course operation in line with their own contexts, so as to deepen the top course program continuously. The criteria for the assessment of top-quality courses are intended to reflect scientifically the basic features of these courses and guide universities to work hard on these courses and build up initiatively top-quality courses with their own distinctive features.

AIM:To develop a HPLC method for determing the contents of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills(Radix et Rhizoma Salviae miltiorrhizae,Fructus Schisandrae chinensis,Rizoma Acori tatarinowii,etc.). METHODS: The samples were extracted with methanol through supersonic wave and then filtered.The determination was performed by HPLC on Shim-pack VP-ODS C_(18) column using methanol-water-acetic acid glacial(65∶35∶0.1) as a mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 220 nm. RESULTS: The linear ranges of schizandrol,schisantherin and deoxyschizandrin were 0.001 2-(0.050 0) ?g(r=1.000 0)、0.001 4-0.057 6 ?g(r=0.999 5) and 0.002 1-0.060 8 ?g(r=0.999 8),respectively.The average recoveries of three components were 99.51%,99.40% and 99.06%,respectively.(CONCLUSION:)The method is accurate,quick,sensitive and can be used for measurement of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills.

Objective To observe the changes of PI3K/Akt signaling pathway and endoplasmic reticulum stress related protein GRP78 and CHOP in CCl4 induced liver fibrosis and to explore their effects on hepatic fibrosis.Methods Thirty SD rats were randomly divided into normal control group, 4 and 8 weeks liver fibrosis group (hypodermic injection of 40% CCl4).Pathological changes of liver tissue was observed by HE staining.The techniques of real-time PCR was applied to detect mRNA of GRP78 and CHOP in liver.Detected expression of Akt1, phospho-Akt1, GRP78 and CHOP protein by western blot.Meanwhile, the cell apoptosis in liver was detected by TUNEL.Results Compared with the normal control group, GRP78 and CHOP mRNA and protein in 4 and 8 weeks liver fibrosis group was increased(P<0.05), while expression of Akt1, phospho-Akt1 in 4 and 8 weeks liver fibrosis group was lower than that in normal control group(P<0.05).Compared with normal control group, the apoptosis of hepatocytes in 4 and 8 weeks liver fibrosis group was elevated (P<0.05).Conclusions PI3K/Akt signaling pathway and endoplasmic reticulum stress may play important roles in the induction of apoptosis of hepatocytes in rats with hepatic fibrosis.

A comparative study on the histological and morphological characteristics and the intrinsic quality of the roots of Huabei Qianhu(Peucedanum harry-smithii),Shaomao Bei Qiau hun (P.harry-smithii var.subglabrum ) and Baihua Qianhu (P.praeruptorum ) was carried out. Results revcaled that the former two Qianhu produced in Gansu are similar to Baihua Qian hu in their main ingredients.Thus the two Qianhu are worthy for further research and develo pm ent.At the same time, it was observed that Baihua Qianhu Produced in Gansu is of iuferior quality and the content of EtOH extraet of its root is slightly lower than that from elsewhere in China.

Objective To clone, locate and differentially analyze the transcription factor Relish gene from Anopheles stephensi, and to examine its signals-modulating action on prophenoloxidase cascade and melanization of Plasmodium yoelii oocysts. Methods Relish cDNA of total mosquitoes was amplified by RT-PCR with degenerated primers. Target PCR product was purified,cloned,sequenced and identified. Special Relish gene was amplified with specific primers from hemocytes or midgut,respectively. Semi-quantitative analysis was made under different feeding conditions. Relish message ribonucleic acid was identified with hybridization in situ. Results One cDNA segment of Relish similar to An.gambiae was acquired from An. stephensi. The same Relish gene was also manifested in the hemocytes and midgut. Marked up-regulation expression of Relish was observed at 6,12,24 or 48 h of Plasmodium yoelii infection and at 12 and 24 h after sucking nitroquine-acetate sucrose solution,that was before inducible oocyst melanization. Relish was also expressed in the hemocytes and midguts by ISH. Conclusion Transcription factor Relish of An. stephensi might play a role in signal modulation of Plasmodium yoelii infection and oocyst melanization.