Objective To study the influence of stay-at-home college students' resilience,self-supporting personality on psychological health state.Methods A sampling survey include 536 college students was done by psychological resilience list,self-supporting personality list,90 samptoms list (SCL-90),225 stay-at-home college students were taken as study group,and 311 non-stay-at-home college students as control group.Then the results were calculated and analyzed by SPSS13.0,and the calculating methods were listed as follows:analysis of variance,chi-square test,correlation analysis,linear regression and so on.Results ① The scores of family supporting,interpersonal independence of stay-at-home college students were apparently lower than those of non-stay-at-homes(F =5.826,P ＜ 0.05 ; F =6.614,P ＜ 0.05 ; F =4.163,P ＜ 0.05 ),but the score of independence was apparently higher than those of non-stay-at-homes(F =3.980,P ＜ 0.05).②The SCL-90 total scores and each factor score of stay-at-home college were narrow higher than those of non-stay-at-home college students,while the difference level still didnt reach significant level( all P＞ 0.05 ).The relevance ratio of apparent psychological symptoms of the two group college students didnt has significent calculating difference( all P ＞ 0.05 ).③ The SCL-90 total score item and most individual factor item of stay-at-home college students had apparent negative correlation with the interpersonal independence factor of their resilience and self-supporting personality (r =- 0.537 ～ - 0.132,P ＜ 0.05 ),while not apparent negative correlation with the individual independence factor(r =-0.118 ～0.105,P ＞ 0.05 ),and also they had very few correlation with the SCL-90 factor item which was relevant with interpersonal flexibility.④Emotion control,psychological resilience and interpersonal independence apparently negatively predict the physical and mental symptoms of those stay-at-home college students,with the predicting rate of 33.7％.Conclusion Stay-at-home experience has effects on the family-supporting,bearing power,independence and interpersonal independence of college students ;Emotion control,psychological resilience and interpersonal self-supporting are the protecting factors of the psychological health condition of college students.

Objective:To observe the biological properties of bone marrow stromal cells(MSCs) in vitro in Wistar rats for inducing MSCs to differentiate into neurons and transplantating MSCs to treat cerebral ischemic stroke.Methods:The Wistar rats for 4 weeks old were sacrified,the bone marrow separated and the MSCs cultured,and the biological properties of the cells observed by phasecontrast microscope.Cell surface markers were evaluated by indirect immunofluorescence cycochemistry staining in primary and different generation cells.Results:95.18% and 94.36% of the third generation cells showed positivity to CD54 and CD44(MSCs surface marker).The percentage of hematopoietic lineage marker CD14 decreased obviously from 23.19% in primal culture of the cells to 2.37% in the third generation.Conclusion:The third generation cells can be used as the seed-cells for study of cells induce and differentiation and the resoure of cells transplantation.

Objective:To study the identification and cultivation of neural stem cells in neonatal rats in vitro.Methods:Cell culture in serum-free medium,indirect immunofluorescence cytochemistry were used.Results:The neural stem cells isolated in newborn rats had the potential of forming clones and serial passage,expressing neuroepithelial stem cell protein(nestin)and differentiating into neurons and astrocytes and oligodendrocytes.Conclusion:Neural stem cells maintain their undifferentiated features and have the capacity of self-renewal,proliferation and pluripotentiality.

Objective:To assess the safety and efficacy of defibrase in the treatment of acute cerebral infarction by a randomized, double-blind, and placebo-controlled clinical trial. Methods:65 cases with acute infarction were randomly allocated to receive either an initial intravenous infusion of defibrase 10IU or placebo 250ml of normal saline within 24 hours of stroke onset. Subsequent infusions of defibrase 5IU or placebo were given on the third and the fifth day respectively. The end points included Clinically Neurological Deficits Scale of Stroke, Barthel Index in 3 months, adverse reaction and the level of plasma fibrinogen(FIB). Results:1.The level of plasma FIB in defibrase group was remarkably declined after treatment whereas the bleeding events and other adverse reactions were not increased as compared with the control group. 2.There were no statistically significant differences at Clinically Neurological Deficits Scale of Stroke at 2 weeks,Barthel Index score between two groups. Conclusion:This study shows that defibrase appears safe and effective in degrading plasma FIB.This study does not show that clinical efficacy of defibrase is superior to those medicines being used at present for acute cerebral infarction.

Objective: To construct short-hairpin RNA(shRNA)expressing vectors aimed at mouse signal transducers and activators of transcription-4/6(STAT4/STAT6). Methods: Target sequences in the middle of STAT4 and STAT6 gene were selected, and then the complementary 65 mer oligonucleotides of each sequence were synthesized. After annealed,the inserts were ligated into the linearized pGenesil-3 plasmid. The plasmids were transformed into DH5? bacteria to amplify and then purified. GAPDH plasmid was constructed as a positive control and targeting none gene was served as a negative control. These purified plasmids were identified by gel electrophoresis after being cut with restriction enzyme and sequencing. Results: The results of gel electrophoresis and sequencing showed that the plasmids were identical with the positive control, and the sequences were identical with what we have inserted in. Conclusion: The vectors which can express siRNA hairpin aimed at STAT4/STAT6 gene have been constructed successfully and it lays the groundwork for the further study.

OBJECTIVE:To establish a method for the determination of main component content in flopropione tablets.METHODS:The content of flopropione was determined by UV spectrophotometry with alcohol as solvent under a detective wavelength of 286 nm.RESULTS:The method showed a good linear relationship in the range of 10～60 ?g?mL-1 (r=0.999 9) and the average recovery rate was 99.96% (RSD=0.62%).CONCLUSION:The method is simple,accurate and applicable for the quality control of flopropione tablets.

AIM: Nuclear P53 localization was considered to be proof of mutation, cytoplasmic staining has also been signalled. In this study, special attention has paid to protein subcelluler localization. A different functional meaning may attributed to tumor biological characteristic and evolution. METHODS: Immunohistochemical approach and flow cytometry were used to explored the relationship between P53 overexpression pattern (nuclear and cytoplas- mic) and G1/S checkpoint function of P53 protein in cell cycle. RESULTS: Two patterns of P53 overexpression are related to disease stage significantly. S - phase fraction (SPF) was higher and G0 - G1 phase fraction was lower in P53 nuclear pattern than cytoplasmic (SPF, 42.42? 5 .45/14. 12? 4 .42, G0 - G1: 53. 04 ? 6. 14／７５. 56 ? 4. 46, respectively P

Objective:To assess the safety and efficacy of defibrase in the treatment of acute cerebral infarction by a randomized,double-blind,and placebo-controlled clinical trial.Methods:65 cases with acute infarction were randomly allocated to receive either an initial intravenous infusion of defibrase 10U or placebo 250ml of normal saline within 24 hours of stroke onset.Subsequent infusions of defibrase 5U or placebo were given on the third and the fifth day respectively.The end points included Clinically Neurological Deficits Scale of Stroke,Barthel Index,fatality rate of stroke in 3 months and 12 months,adverse reaction and the level of plasma fibrinogen(FIB).Results:(1)The level of plasma FIB in defibrase group was remarkably declined after treatment whereas the bleeding event and other adverse reaction were not increased as compared with the control group.(2)There were no statistically significant differences at Clinically Neurological Deficits Scale of Stroke at 2 weeks,Barthel Index score and fatality rate at 3 months between two groups.(3)The recurring rate of stroke in control group was higher than that in defibrase group at 12 months(P

Objective:To report the clinical characteristics and diagnoses of diabetic demyelinating neuropathy.Methods:Five cases were systematically studied based on the clinical observations and data from aided examination.Results:All the five patients with diabetes mellitus were more than 50 years old.The clinical manifestations included predominantly motor symmetric weakness,proximal and distal,associated with areflexia and a relapsing or a chronic progressive course.The five cases were indistinguishable from idiopathic chronic inflammatory demyelinating polyneuropathy(I-CIDP) in clinical picture and electrophysiology.They responded to glucocorticosteroid treatment,plasma exchange and intravenous immunoglobulin.Conclusion:Diabetic demyelinating polyneuropathy is one of several types of diabetic peripheral neuropathies.

Bacteria ; genetics ; growth & development ; Biofilms ; Genetic Heterogeneity