Objective In order to compare of consistency of results between URIT‐1500 and AIKELAI AX‐4030 urine drychemistry analyzers and for satisfy the need of urine routine test will choose in the reference range of the optimal urine drychemistry analyzers .Methods During the testing time from March 6 ,2015 to April 4 ,2015 ,random have been collected 378 cleaning midstream urine specimens form Luzhou medical college affiliated hospital were detected by the two kinds of urine drychemistry analyzers in parallel .Positive detection rates of each project were analyzed statistical by chi‐square test and counting accordance rate ,general accordance rate ,and the kappa value completely .Results The percentage of variance difference of specific gravity(SG) between the two analyzers was 0 .564% (<1% );Positive detection rates(P value) of WBC ,BIL ,PRO ,BIL between the two analyzers were<0 .05 ,the results were statistical significantly ;Positive detection rates(P value) of GLU ,NIT ,KET ,URO were>0 .05 ;the general accordance rate of LEU ,GLU ,NIT ,KET ,pH were>90% and BLD ,PRO ,URO ,BIL were>70% .Com‐pletely accordance rate except GLU ,NIT ,KET were>90% and the rest of six projects trend lower than normal rate .The kappa value of LEU ,BLD ,GLU ,KET ,URO ,BIL were>0 .40 and PRO ,pH were>0 .30 .Conclusion There is a poor consistency be‐tween the two kinds of urine drychemistry analyzers in the same test condition .It can not to use two different kinds of analyzers in same hospital and should choosing the optimal urine drychemistry analyzers in reference range need on the basis of the particular situations of hospital .Urine drychemistry analyzers is used only for screening ,and the results should be reviewed by urine sediment microscopy in order to meet clinical needs .

OBJECTIVE:To observe the curative and preventive effects of reco mbinant basic fibroblast growth factor(rbFGF)on radio mucitis.METHODS:120patients with head and neck carcinoma were randomly divided into the therapy group and control group with60patients in each group,1week after radiotherapy till the end,the therapy group were given1ml of rbFGF by oral spraying5puff,3times daily after meal and the patients in the control group mouthwash with compound borax solution3times daily after meal.RESULTS:Radio mucitis in therapy group was less serious than that in the control group(P

OBJECTIVEThis study aimed to explore the effect of the up-regulation of Notch1 on osteoclastogenesis induced to osteoclasts by receptor activator for nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factors (MCSF) in vitro.

METHODSThe bone marrow stem cells (BMSCs) of Rosa(-notch1) mice were cultured and induced to osteoclasts by RANKL and MCSF. The BMSCs were transfected with the Ad-Cre-green fluorescent protein (GFP) virus or Ad-GFP virus. Total RNA from cells was extracted, and the gene expression levels of Notch1, Notch2, Notch3, Notch4, Deltal, Delta3, Delta4, Jagged1, Hes1, and tartrate resistant acid phosphatase (TRAP) were detected at the defined stage by reverse transcription-polymerase chain reaction (RT-PCR). Osteoclast formation was analyzed by TRAP assay.

RESULTSThe number of TRAP-positive multinuclear cells of the experimental group significantly decreased compared with that of the control group. The mRNA expression levels of Notch1, Notch3, Jagged1, Delta3, and Hesl of the experimental group were significantly higher than those of the control group, whereas the TRAP mRNA expression of the experimental group was significantly lower than that of the control group (P<0.05).

CONCLUSIONUp-regulation of Notch1 inhibit osteoclastogenesis of BMSCs induced by RANKL and MCSF in vitro.

Animals ; Cell Differentiation ; Cell Line ; In Vitro Techniques ; Macrophage Colony-Stimulating Factor ; Mice ; Osteoclasts ; RANK Ligand ; Receptor Activator of Nuclear Factor-kappa B ; Receptor, Notch1 ; metabolism ; Receptor, Notch2 ; Up-Regulation ; physiology

OBJECTIVETo evaluate the effect of the oral-appliance in the treatment of ED induced by obstructive sleep apnea-hypopnea syndrome (OSAHS).

METHODSThis study included 40 cases of OSAHS-induced ED. Based on the apnea-hyponea index (AHI) and lowest SaO2, we divided the patients into groups A (AHI < or = 30/h and lowest SaO2 > or = 80%) and B (AHI > 30/h and lowest SaO2 < 80%), and treated them with the oral-appliance for 3 months, followed by evaluation of the therapeutic effect using polysomnography, IIEF-5 questionnaire and Sexual Encounter Profile (SEP) diaries.

RESULTSThirty-seven of the patients accomplished the treatment, 16 in group A and 21 in group B. Compared with the baseline, the AHI was significantly decreased in both groups A (21.4 +/- 7.3 vs 14.2 +/- 6.6, P < 0.05) and B (51.4 +/- 9.5 vs 29.7 +/- 8.1, P < 0.05) after treatment, the IIEF-5 scores increased, even more remarkably in B than in A (3.36 +/- 2.48 vs 1.12 +/- 1.27, P < 0.05). The positive rates of SEP2 and SEP3 were markedly lower in A than in B (37.5% vs 76.2% and 25.0% vs 61.9%, P < 0.05).

CONCLUSIONThe oral-appliance can improve the erectile function of OSAHS-induced ED patients, particularly for those with severe OSAHS.

Adult ; Aged ; Erectile Dysfunction ; complications ; therapy ; Humans ; Male ; Middle Aged ; Orthodontic Appliances ; Sleep Apnea, Obstructive ; complications ; therapy ; Treatment Outcome

Objective To investigate the effects of high molecular bio-degradable film on preventing peritoneal adhesion.Methods One hundred and twenty rats were divided into control group,non-bio-degradable film group and polycaprolactone(PCL) group.Animal models of peritoneal adhesion made by operations on rats serve as control group.In the study,non-bio-degradable film and bio-degradable,namely PCL films sensitive to pseudomonas enzyme,were put into the opening of peritoneum.The effect of the films on peritoneal adhesion was examined on 1,3,7 and 30 d after operation.Results Peritoneal adhesion was found in all the rats of control group and very low in PCL group and non-bio-degradable film group.Conclusion PCL film can effectively prevent the formation of postoperative peritoneal adhesion.

Objective To investigate the level change of interleukin-4(IL-4) and IL-12, and their effect on airway inflammation in rat asthma. Methods Rat model of asthma was set up by ovalbumin sensitization. Total cell count and eosinophil count in branchoalveolar lavage fluid(BALF) were performed, and levels of IL-4 and IL-12 in BALF were measured by ELISA. Results After ovalbumin sensitization, airway occurred obvious inflammation. Eosinophil count began increasing at 1 hour after sensitization, obviously increased at 8 hours, reached a maximum at 24 hours, started declining at 96 hours, and decreased to near normal range at 7 to 14 days. The changes of total cell count had the same tendency as eosinophil count changes. The level of IL-4 in BALF elevated at 1h after sensitization, reached a maximun at 8 to 96 hours, and reduced to near normal level at 7 to 14 days. The changes of IL-12 in BALF had the same tendency as IL-4 level changes. Eosinophil count was positively related with IL-4 level(r=0.74,P0.05). There was a close correlation between IL-4/IL-12 ratio and eosinophil count (r=0.85,P

OBJECTIVE:To discuss the relevant risk factors of antibiotic-associated colitis (AAC) and provide objective basis for formulating measures to prevent AAC.METHODS:Of the total of 3 520 patients who received antibiotics in our hospital during 2007～2008,those with AAC were assigned to trial group and those without AAC to control group.The factors that might influence AAC were subjected to single-factor analysis and Logistic regression analysis.RESULTS:Of the total 3 520 patients,AAC was noted in 190 patients (5.4%).Through single-factor analysis,10 factors were found to be associated with AAC;and through non-conditional Logistic model analysis,the following 6 major risk factors were found to be associated with AAC,i.e.,older age,fasting,severe cases,using numerous kinds of antibiotics,long-term use of antibiotics and use of antacid.CONCLUSION:AAC are associated with multiple factors,but its incidence can be prevented or reduced through rational use of antibiotics,monitoring on elder cases,supplement of probiotics,early enteral nutrition and food intake,control of the medication duration of antacids,reduction of invasive operation for the severe cases and avoiding frequent change of antibiotics,etc.

Objective To analyze the incidence rate of congenital malformations in Xintai City from January 2010 to December 2013,and to investigate the risk factors for neonatal malformations. Methods A total of 21 463 ca-ses of perinatal infants pregnant for 28 weeks later to postpartum 7 d in Xintai city from January 2010 to December 2013 were retrospectively analyzed. The congenital malformations detection data were analyzed. The risk factors were analyzed by univariate and Logistic analysis methods. Results There were 281 cases with congenital malformations were identi-fied out,and the congenital malformation rate was 1. 31%(281 / 21 463 cases). The incidence rates of multi - finger (toe)[0. 24%(52 / 21 463 cases)],cleft lip[0. 23%(49 / 21 463 cases)],and congenital heart disease[0. 22%(47 /21 463 cases)]were the main congenital malformations. Total malformation rates in the year of 2010,2011,2012,and 2013 were similar(1. 26% ,1. 25% ,1. 33% ,1. 26% ,Z = - 1. 826,P = 0. 068). Univariate and Logistic regression analysis results showed that birth weight,parity,births number,gestational age,educational level,residence,income,ill-ness history,exposure to pesticides,hepatitis,influenza,severe vomiting of pregnancy,threatened abortion,threatened premature,contraceptives,smoking history,drinking history,and father's chronic disease were the risk factors for neo-natal congenital malformations( χ2 = 10. 212,4. 299,5. 860,5. 278,10. 422,9. 327,15. 680,127. 395,245. 735, 74. 141,718. 876,96. 414,77. 770,11. 300,9. 126,74. 927,68. 283,5. 450,P = 0. 001,0. 038,0. 015,0. 022, 0. 001,0. 002,0. 000,0. 000,0. 000,0. 000,0. 000,0. 000,0. 001,0. 001,0. 003,0. 000,0. 000,0. 020 ). Conclusions Neonatal congenital malformation is mainly determined by genetic and environmental factors. For childbearing age and pregnant women,targeted health education should be strengthened to reduce the incidence rate of congenital malformations.

As a member of tyrosine kinases(TK) family,Epidermal growth factor receptor(EGFR) has an activity of intrinsic protein tyrosine kinase,which plays an essential role in the regulation of signal transduction in the cells.Due to the abnormal expression of EGFR-TK,the certain type cancers may developed and progressed.Based on that,the inhibitors of EGFR-TK could be effective medicines for the treatment of cancer.The EGFR-TK domain was amplified by RT-PCR with RNA of HUVCEs cells as the template and expressed in E.coliBL21(DE3) using plasmid pET30a as vector.The recombinant protein was purified with the affinity chromatography(Ni-NTA),which was identified to have kinase activity catalyzing the substrate phosphorylated with ATP in the enzymatic reaction.Using the recombinant EGFR-TK as target,the screening model for enzymatic inhibitors was constructed.

Objective To investigate the feasibility and efficacy of combined preoperative chemora- diation program followed by radical surgery in bulky early or moderate uterine cervical cancer.To determine the incidence and predictive value of rasidual disease in the radical hysterectomy specimens after CCR. Methods Thirty-five patients with uterine cervix carcinoma from January 2001 to September 2003 were treated with preoperative external beam radiotherapy to 3060 cGy in 4 weeks.Patients received concurrent continuous infusion cisplatin(20 mg/m~2)on day 1,8,15,22 of four weeks and 5-Fu(4 g)chemotherapy during the first four days and the last four days of the radiation course.Radical surgery was not performed until 4 weeks after the completion of the preoperative treatment.Results Clinical response was 85.71%,complete response,54.29 %;partial response,31.43 %.The analysis of the surgical specimens showed 19 patients had revealed residual disease and 16 patients had revealed no residual disease,the complete pathological response hed been 45.71%.Three-year survival rate was 87.14 % in 35 patients.Three-year survival rate of residual disease patients(76.32 %)was significantly lower than that of no residual disease(100.0 %)(P=0.0358). Three-year survival of cervical stromal invasion less than 1/2 and invasion extra stromal was 100.0 %, 41.67 % respectively(P=0.0109);three-year survival of lymph-vascular space involvement and no lymph- vascula space involvement was 0,85.29 %,respectively(P=0.0148).Conclusion Combined preoperative chemoradiation program followed by radical surgery in bulky early or moderate cervical cancer could reveal an effective efficacy with a tolerant complication.Residual disease is an independent and strong predictive factor.