0.05).Conclusions:1.The acute rise in FFAs level for a short term can inhibit ACTH and cortisol secretion in healthy male adults,but with prolonged FFA elevation this inhibition last less than 24 hours.2.The acute FFAs rise has no influence on ACTH or cortisol circadian rhythm.

In the present study, Mongolian gerbil was used to make delayed neuronal damage (IND) animal model. In 6 different brain regions, 5 kinds of biochemical indexes in the aspect of free radical metabolism were measured at 7 time phases during ischemia and reperfusion. The results showed that, after transient cerebral ischemia and during reperfusion, some unique changes appeared tn hippocampal CA1 sector, which were characterized by the persistent increase of free radical content and persistent decrease of the Mn-SOD activity. The fluctuating changes could be seen in Cu, Zn-SOD activity and lipid peroxide content. There were no significant changes in the brain regions other than CA1 sector. It is concluded that the disturbance of free radical metabolism play a key role in the occur. fence of DND. It was also observed that Aniracetam, Helicid and Nieardipine had some control effects to a certatn extent on the free radical disturbance above in hippocampal CA1 sector of DND animals.

Objective To evaluate apelin level in patients with obesity and different glucose tolerances, investigate relevance of apelin with body mass index(BMI), blood lipid and blood glucose. Methods According to different glucose results of oral glucose tolerance test, 120 subjects were divided into three groups: normal glucose tolerance(NGT),impaired glucose tolerance(IGT) and type 2 diabetes(T2DM). All of the subjects were also divided into obese and non-obese subgroups based on their BMI(≥25kg/m~2 or ＜25kg/m~2 ). Plasma apelin levels were compared among groups of NGT, IGT and T2DM. Fasting plasma glucose(FPG)and 2h postprandial plasma glucose (2hPG) after OGTT, lipid profile and BMI were measured. Results Both fasting and 2h postprandial plasma apelin had significant differences in these groups (P<0.05,or P<0.01). The overweight and obese subgroups in the IGT group and diabetes group had higher apelin levels than their counterpart subgroups in the NGT group. BMI, FPO, systolic blood pressure(SBP) were independent influencing factors for apelin (P<0.05 ,or P<0.01). Conclusions Plasma apelin level is related to obesity and type 2 diabetes.

Resiliency is individual's capability to cover difficulties and make a good adaptation.This research reviews the researches on resiliency abroad.It summarizes the practical researches of resiliency from 3 aspects: ① The concept of resiliency;②The structure and intension of resiliency;③The measurement of resiliency.On this basis,it raises some questions in resiliency researches and puts forward the future direction.

Objective To investigate the curative effects of keyhole microsurgical craniotomy to hypertension cerebral hemorrhage. Methods Treatment and curative effects were retrospectively reviewed in 68 patients of hypertension cerebral hemorrhage from Jan. 2008 to Dec. 2010. Results After treatment with keyhole microsurgical craniotomy, in 68 patients, besides 1 case death, in 3-12 month follow-up, level Ⅰ (complete recovery) 22 occupies 32.3%, level Ⅱ (self care) 20 occupies 29.3%, level Ⅲ (requires assistance) 19 occupies 27.9%, level Ⅳ (confined to the bed but conciousness) 4 occupies 5.9%, level Ⅴ (vegitative survival) 2 occupies 2.9%. Conclusion The curative effect of keyhole microsurgical craniotomy to hypertension cerebral hemorrhage is remarkable.

Background and purpose: More than 90% of cancer patients are incurable because of drug resistance. Activation of PI3K/Akt/mTOR signaling pathway in breast cancer, as a target for chemotherapy drugs has become a hot topic of breast cancer treatment. This study aimed to investigate the effect and mechanism of XCL1 on the proliferation of drug-resistant breast cancer cell, whether is related with the mTOR signaling pathway. Methods:Established gemcitabine-resistant breast cancer cell lines (MDA-MB-231/Gem). CCK8 to detect the proliferation of MDA-MB-231 and MDA-MB-231/Gem, RT-PCR and ELISA to determine the XCL1 expression level of the two cell lines, Western blot to detect the expression of mTOR. Results:Compared with MDA-MB-231, MDA-MB-231/Gem showed an enhanced proliferative capacity. The expression of XCL1 was increased in the resistant cell lines. Both of protein level and phosphorylation level of mTOR increased in drug-resistant cell lines. The MDA-MB-231 added exogenous XCL1 for 24 h, showed an enhanced cell proliferation. Adding anti-XCL1 antibodies in MDA-MB-231/Gem could reduce cell proliferation and treating MDA-MB-231/Gem with the mTOR inhibitor could also reduce cell proliferation, as well as the XCL1 expression level. Conclusion:XCL1 promotes the proliferation of drug-resistant breast cancer cells mediated by activation of the mTOR pathway.

Objective To identify a detailed biological characterization of mesenchymal stem cells (MSCs) isolated from hu‐man umbilical cord(UC) tissue regarding their morphology ,immunophenotype ,purity and proliferative capacity and establish a rea‐sonably cultured and amplified system .Methods After stripping off arteries and veins ,the remaining parts of umbilical cord were cut into 1 mm3 small sections and cultured with DMEM/F12 containing 10% fetal bovine serum .Adhere cells were obtained and the morphology of the cells was observed under inverted phase contrast microscope .The growth curves of them were drawn by CCK‐8 and the cell cycle and surface antigens (CD29 ,CD73 ,CD90 ,CD105 ,CD31 ,CD14 ,CD34 ,CD45 ,CD11b ,HLA‐DR) were detected by flow cytometry .Results Seven to ten days after primary culture ,adhere cells came out of fragments .The MSCs harvested were a high purity and mainly presented as a fibroblast‐like morphology .UC‐MSCs had a strong ability of proliferation through the cell growth curve .The special surface antigens CD29 ,CD73 ,CD90 ,CD105 were positive expression ,while CD31 ,CD14 ,CD34 ,CD45 , CD11b ,HLA‐DR were negative .More than 80% cells of MSCs were found at G0/G1 phase .Conclusion Human UC‐MSCs could be cultured and proliferated in vitro .

OBJECTIVETo explore the features of various mediastinal lymphadenopathies using computed tomography perfusion (CTP).

METHODSCTP parameters (CTPs) of the selected mediastinal nodes from 59 patients with pathology-proven malignant lymph nodes and of those from 29 patients with clinically diagnosed or pathology-proven inflammatory lymphadenopathies were collected. Patients were divided into subgroups by etiology and phase of primary disease, including different pathological malignant nodes and diverse inflammatory nodes. CTPs were defined as blood flow (BF), blood volume (BV), mean transit time (MTT), permeability (PMB), and time to peak (TTP). Differences of CTPs were compared between malignant and benign nodes, and among subgroups, respectively.

RESULTSIn the mediastinum, no significant differences of CTPs were found between malignant and benign groups (all P>0.05), the same for subgroups of malignant nodes (all P>0.05). Acute lymphadenitis had higher BF and BV than chronic inflammatory, lymphoid tuberculosis, sarcoidosis and malignant nodes. The BF of malignant nodes was markedly slower than that of acute lymphadenitis (P=0.01), but faster than chronic inflammatory nodes (P=0.04) and sarcoidosis (P=0.03), with no significant difference compared with lymphoid tuberculosis. Pneumonia-complicated lymphoid tuberculosis showed the longest MTT while sarcoidosis displayed the shortest MTT, and inflammatory nodes, lymphoid tuberculosis without complicated pneumonia and malignant nodes had moderate MTT.

CONCLUSIONCTPs show promising potential in distinguishing various lymphadenopathies in the mediastinum, but more studies are needed to improve their specificity.

Adult ; Female ; Humans ; Lymph Nodes ; diagnostic imaging ; Lymphatic Diseases ; diagnostic imaging ; Male ; Mediastinal Diseases ; diagnostic imaging ; Perfusion ; Retrospective Studies ; Tomography, X-Ray Computed ; methods

OBJECTIVETo assess the quality of reporting by randomized controlled trial (RCT) related to dental implants in China during 2000 to 2012 by using the revised Jadad scale and consolidated standards of reporting trials (CONSORT) (2010) statement.

METHODSThe following electronic databases were searched: Chinese Biomedical Literature Database, Database for Chinese Technical Periodicals, China National Knowledge Infrastructure, PubMed, and EMBASE. A total of 19 journals of stomatology in China were also searched manually. The qualities of RCT with dental implant published between 2000 and 2012 were assessed using CONSORT (2010) statement and revised Jadad scale.

RESULTSTwenty-eight RCTs related to dental implants were identified. The quality of reporting in 28 articles was low. The mean revised Jadad score was 1.29 ± 0.71 and the CONSORT (2010) score was 9.75 ± 3.60.

CONCLUSIONThe methodological qualities of the included studies on dental implants are generally low, and reporting quality remain unsatisfactory.

China ; Dental Implants ; Humans ; Publishing ; Randomized Controlled Trials as Topic

Hedgehog family proteins are important morphogens which mediate the development of embryo as well as the carcinogenesis in adults. Inappropriate activation of Hedgehog signaling pathway contributes to numerous human cancers. The Hedgehog signaling pathway is considered to be involved in the molecular mechanism of the invasion and metastasis of the tumors, and to facilitate the metastasis of tumors through signaling pathways interaction. It is suggested that a potential therapeutic strategy pharmacologically targeting Hedgehog-dependent tumors may be developed.