BACKGROUND: Research on muscular fatigue has extensive value of application in fundamental research about neuromuscular system, handicapped rehabilitation engineering, objective evaluation on physiotherapeutic effect, scientific training of athletes and ergonomics etc.OBJECTIVE: To study partial muscular fatigue by using AR model parameter of needle electrode electromyography (NEMG) signal and try to reveal the quantitative relationship between local muscular fatigue process and AR model coefficient of NEMG signal.DESIGN: Human NEMG signal was taken as the subject, the changing rule of myoelectric characteristics parameter in local muscular fatigue process was studied.SETTING: NML Laboratory of China University of cience and Technology.PARTICIPANTS: 4 eases of NEMG signals were obtained from NEMG signal collection system manufactured by NML Laboratory of China University of Science and Technology. Four volunteers that selected were healthy males and their tibialis anterior muscles were tested.METHODS: Based on the theory of random signal parameter model,NEMG signals were modeled and parameter was selected, the trend of parameter of NEMG signal changing with the time increasing was studied in the muscular fatigue process. Relative programs from MATLAB language toolbox were adopted for programming.MAIN OUTCOME MEASURES: The trend of α1 parameter of NEMG signal changing with the time (fatigue process) increasing. RESULTS: There was a correlation between local museular fatigue of human body and the changing trend of α1 parameter of NEMG signal tested from the muscle, that is, α1 parameter of NEMG signal increased with the time (fatigue process) increasing.CONCLUSION: By means of the increasing trend of α1 parameter of NEMG signal with the time (fatigue process) increasing, muscular fatigue state can be better evaluated.

Biomedical engineering is an inter-discipline developed from the combination of science,engineering and biomedicine. This paper aims to point out the advantages and problems of the biomedical engineering faculty through the analysis of professional background of medical college,and propose solutions to this problems.

Objective To investigate the effect and clinical application of two kinds of endoscopic titanium clip in treatment of iatrogenic or iatrogenic perforation of duodenal descending part. Methods For 15 cases of perforation of duodenal descending part, according to the specific location of the perforation, select different endoscopic, compare the closing efficiency and success rate. Results 8 cases closed under gastroscopy in 15 cases of descending part of duodenum perforation, successfully closed in 7 cases, success rate was 87.5 %; 7 cases closed under duodenoscopy, successfully closed in 7 cases, the success rate 100.0%. 14 cases successfully closed by endoscopic titanium clip in 15 cases, 1 case failed, the success rate was 93.3 %. The effective titanium clip quantity, invalid (loss) titanium clip quantity and the closing time between the two groups has no statistically significance (P > 0.05). Conclusion It is safe and effective to use two kinds of endoscopic titanium clips in treatment of iatrogenic or iatrogenic duodenal per-foration.

Cervical Vertebrae ; Dura Mater ; Epidural Space ; Humans ; Spinal Cord Neoplasms ; surgery

Objective: To develop a new method for the simultaneous determination of two hydrophilic components and two lipophilic components of Radix et Rhizoma Salviae Miltiorrhizae. Methods: The HPLC-DAD method was employed using a column of Agilent Zorbax TC C18 (4.6 mm × 250 mm, 5 μm) with a mobile phase of methanol -2% acetic acid. The gradient elution program was as follow:0-15 min, 30% B-40% B; 15-20 min, 40% B-60% B; 20-25 min, 60% B-90% B; 25-40 min, 90% B. The detection wavelength was set at 281 nm and the temperature was 35°C. Results: The linearity was obtained over 3.76-120.20 μg · ml-1 (r=0.999 9) for rosmarinic acid, 34.20-109 4.5 μg · ml-1 (r=0.999 9) for salviamolic acid B, 0.64-20.32 μg · ml-1 (r=0.999 9) for clyptotanshinon, and 1.02-32.72 μg · ml-1 (r=0.999 6) for tanshinone II A. The RSDs of precision and stability of the sample were both less than 1% in 48 hours. The average recovery was between 99.72%-100.63%. Conclusion: The present method is simple and has satisfactory efficacy; it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.

Objective:To study the effects of WAK on immune function in mice.Methods: After different doses of WAK were given to mice ig, its effects on delayed type hypersensitivity (DTH), hemolysin production, NK and LAK cell killing activity were observed.Results: WAK could enhance DTH and hemolysin production in immunosuppressive mice and improve NK and LAK cell killing activity in mice.Conclusions: WAK could exhibit its antitumor effect by increasing immune function.

Objective To introduce a new technique of treating tibial spinal fractures in children under arthroscope, and to assess its clinical outcomes. Methods From February 2001 to July 2003, 6 child patients with tibial spinal fracture were treated arthroscopically. The reduction and fixation were completed under the arthroscope. The wire was linked with the suture outside the joint, and then was pulled into the joint to fix the fracture fragment through the fundus of anterior cruciate ligament (ACL). Results The patients were followed up for 6 to 20 months. All the fractures healed without displacement 4 weeks after operation. 3 months postoperatively, the motion range of the knee returned to normal, and no knee instability was observed. The results of ADT (anterior drawer test) and Lachmanns sign were negative. Conclusion This technique is the first choice in treatment of tibial spinal fracture in children, because it facilitates the diagnosis and treatment of complicated knee problems and provides accurate reduction and reliable fixation. Besides, it is minimally invasive, simple and convenient.

Objective To observe the sleeping time and analyze its impact on the psychological state and quality of life of patients with type 2 diabetes .Methods Convenience sampling was used to recruit 365 patients with type 2 diabetes in Jiangsu Province Hospital on Integration of Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine between April 2014 and April 2015 .All the patients were divided into two groups according to the six-hour cut-off point of sleeping time .They were investigated by means of World Health Organization Quality of Life-BREF ( WHOQOL-BREF), Diabetes Distress Scale ( DDS) and General Self-Efficacy Scale ( GSES).Data were analyzed by t-test, chi-square test, Mann-Whitney U test and multivariate regression analysis .Results The average sleeping time was 7.03 h, and the sleeping time of 109 patients was less than 6 h.Patients with less sleeping time had lower quality of life and self-efficacy scores as well as higher diabetes distress scores than those with sleeping time more than 6 h.The differences were statistically significant (P<0.05).Regression analysis showed that sleeping time was the factor of quality of life (β=0.117, P=0.047), self-efficacy (β=0.136, P=0.024) and diabetes distress(β=-0.118, P=0.046).Conclusion Sleeping time affects the psychological state and quality of life of patients with type 2 diabetes.The medical should pay more attention to the quality of sleep of such patients .

Objective To evaluate the effect of “flying kite” technique and detachable balloon on embolization of carotid-cavernous fistulas (CCF). Methods 9 cases of CCF were treated by using“flying kite”technique, and 6 cases of CCF were treated by using detachable balloon. Results In “flying kite” treatment group, 8 cases were completely cured, and 1 case was partly cured. In detachable ballon treatment group, all 6 cases were completely cured once. Conclusion The“flying kite”technique was simple method for embolization of CCF, which didn't need special instruments and materials, and was easy to use in the basic hospitals, but it could obstruct carotid artery and lead to CCF recurrence. The embolization of CCF by using detachable balloon had a higher curative rate and recurred rarely, but it needed special instruments and skills. Detachable balloon embolization of CCF is a reliable method for the treatment of CCF. [

An actinomycete strain P-13, with antimicrobial activity against muskmelon bacterial spot pathogens, was isolated from the muskmelon rhizosphere soil samples in Xinjiang. The strain P-13 was identified as Streptomyces rochei based on morphological, physiological characteristics and 16S rRNA sequence analysis. The agar diffusion bioassay showed that the diameter of inhibition zone against Acidovorax avenae subsp. citrull BFB and Pseudomonas syringae pv. Lachrymans P4 was above 19 mm and 17 mm, respectively. The antimicrobial substances obtained from strain P-13 were demonstrated to be alkaline and water-soluble compounds according to paper chromatogram analysis and exocellular metabolites. Furthermore, it was stable to be treated by 100?C for 10 min, pH 6 for 6 h, or ultraviolet treatment for 7 h. Moreover, it was insoluble in organic solvents, such as petroleum benzine, diethyl ether, and acetic ether.