The basic medical subjects teaching for international medical students in the local university is a very important component in the medical subjects of the higher education.Our university achieves good teaching effects in reforming the content of the international medical students,renovating in the cultivating syllabus,teaching students in accordance of their aptitude and improving the teaching patterns and methodology.

Objective To investigate the effects of atorvastatin combined with Naoxintong capsules on serum lipid level and hish sensitivity C-reactive protein(hs-CRP)in patients with acute coronary syndrome(ACS).Methods 82 ACS patients were randomly divided into observation group(10 mg/d of atorvastatin and 4 capsules 3 times of Naoxintong daily,n=41) and control group(20 mg/d of simvastatin daily,n=41).The levels of serum lipid and hs-CRP were detected before and after treatment.Results 8 weeks after treatment,the levels of serum hpid[TC (4.21±0.87)mmol/L,TG(1.99±0.48)mmol/L,LDL-C(2.71±0.83) mmol/L] and serum hs-CRP(5.63±1.38)mg/L in observation group were significantly lower than those in control group[TC(4.89±0.91)mmol/L,TG (2.58±0.52)mmol/L,LDL-C(3.28±0.89)mmol/L],and serum level HDL-C(1.89±0.47)mmol/L was higher than that in control group(1.63±0.45)mmol/L(t=2.55-4.36,P<0.01～0.05).Conclusion Adjuvant therapy of Naoxintong could promote the levels of serum lipid and hs-CRP in patients with ACS.and offer a better therapeutic effect in these patients.

BACKGROUND:In mammalian cells,introduction of double-stranded small interfering RNA(19-25 bp)can cleave and destroy the cognate RNA,which can result in suppression of gene expression.OBJECTIVE:To construct siRNA expression plasmid for interference angiotensinogen(AGT),thereby,to resist AGT expression in adipose cells.METHODs:The mRNA sequence of AGT gene was searched from NCBI(NM000029).Utilize of GenScript siRNA technology,AGT-siRNA oliaonucletides were chemically synthesized and inserted into pRNAT-U6 1/Neo vector after annealing,then transformed into TOP10.The recombinant plasmid was identified by restriction endonuclease and DNA sequencing.RESULTS AND CONCLUS1ON:The recombinant plasmid psiRNAT-U6.1/Neo-AGT was obtained by connecting 19 bp segment containing AGT-mRNA sequence to pRNAT-U6.1/Neo After EcoR Ⅰ and Hind Ⅲ digestion.351 bp segment was obtained from empty vector.and 397 bp fragment band was obtained form recombinant plasmid,which was coincidence to the expectation.DNA sequencing showed Targeting siRNA oligonucleotides were correctly inserted into the eukaryotic expression vector pRNAT-U6.1/Neo without base mutation.The interference vector psiRNAT-U6.1/Neo-AGT was successfully constructed.

Objective To investigate clinical effects and safety difference of cholesterol absorption inhibitor associated with atorvastatin for high dose on elderly patients with coronary heart disease and poor lipid controlling. Methods 240 elderly patients with coronary heart disease and poor lipid controlling were chosen and randomly divided into 3 groups including group A (80 patients)with atorvastatin for conventional dose,group B (80 patients)with atorvastatin for high dose and group C (80 patients)with atorvastatin for high dose associated with ezetimibe for cholesterol absorption inhibitor;and the compliance rate of total cholesterol (TC)and low density lipoprotein cholesterol (LDL-C),serum lipids before and after treatment and the incidence of adverse reaction of 3 groups were compared. Results The compliance rate of TC of group A,group B and group C were separately 40.00%(32/80),62.50%(50/80)and 96.25%(77/80);the compliance rate of LDL-C of group A,group B and group C were separately 51.25%(41/80),81.25%(65/80)and 98.75%(79/80);the compliance rate of TC and LDL-C of group C was significant better than that of group A and group B (χ2 =58.28,27.83,48.13,13.61,all P<0.05).The levels of TC,TG,LDL-C and HDL-C of group A after treatment were separately (5.35 ±1.26)mmol/L,(2.10 ±0.83) mmol/L,(2.85 ±0.93)mmol/L and (1.23 ±0.67)mmol/L;the levels of TC,TG,LDL-C and HDL-C of group B after treatment were separately (4.97 ±1.02)mmol/L,(2.05 ±0.81)mmol/L,(2.47 ±0.86)mmol/L and (1.20 ± 0.65)mmol/L;the levels of TC,TG,LDL -C and HDL -C of C group after treatment were separately (3.84 ± 0.82)mmol/L,(1.74 ±0.56)mmol/L,(2.06 ±0.71)mmol/L and (1.24 ±0.52)mmol/L.The levels of TC,TG and LDL-C of group C after treatment was significant better than group A and group B (F=3.80,4.94,4.21,all P<0.05).There was no significant difference in level of HDL-C among 3 groups after treatment(P>0.05).The incidences of adverse reaction of group A,group B and group C were separately 1.25%(1/80),3.75%(3/80)and 2.50%(2/80);there was no significant difference in incidence of adverse reaction among 3 groups(χ2 =1.03,P>0.05).Conclusion Cholesterol absorption inhibitor associated with atorvastatin for high dose on elderly patients with coronary heart disease and poor lipid controlling can efficiently control blood lipid levels,improve compliance rate of blood lipids,and did not increase the risk of adverse reaction.

Objective To investigate the effect of ethyl pyruvate (EP) on the expression of caspase-3 in the hippocampus and the learn-ing and memory ability in neonatal rats with hypoxic ischemic brain damage (HIBD). Methods 90 Wistar rats of 7 days old were randomly divided into sham operation group (n=15), HIBD group (n=15), EP1 group (n=15, 10 ml/kg), EP2 group (n=15, 30 mg/kg), EP3 group (n=15, 50 mg/kg) and EP4 group (n=15, 100 mg/kg). The model was established with Rice's method. 30 minutes before operation, and every 24 hours after operation, EP groups were injected with 10 ml/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg EP in abdomen respectively, for 2 weeks. Af-ter treatment, the caspase-3 positive cells were observed by immunohistochemical staining, and the latency and the times crossing the target quadrant were tested by Morris water maze test. Results The caspase-3 positive cells were less in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). The latency and the times crossing the target quadrant were better in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). Conclusion Ethyl pyruvate can de-crease the expression of caspase-3 in hippocampus, and improve the ability of memory and learning in neonatal rats with HIBD.

Drug addiction is a chronic recrudescent brain dis-ease. Various addictive drugs acting on the reward system result in rewarding effects through changes in neurotransmitter patholog-ical release. Among these monoamine neurotransmitters, 5-hydroxytryptamine, norepinephrine and dopamine play key roles in drug addiction. This paper reviews, from a comprehensive perspective, the roles which monoamine neurotransmitters play in the drug addiction and the process of getting addictive.

Objective To investigate the relationship between Methotrexate (MTX) and its cognitive dysfunction,and to explore the possible mechanism of neurotoxicity induced by MTX.Methods Thirty healthy male SD rats weighting 180-220 g were divided into 3 groups using random number table:control group,60 mg/kg MTX (MTX60) group and 100 mg/kg MTX (MTXt00) group,with 10 rats in each group.The rats in MTX60 group,MTX100 group received 60 mg/kg MTX and 100 mg/kg MTX,respectively.The rats in control group accepted the same volume of 9 g/L saline injection as MTX group.Spatial memory of rats was evaluated by using Morris water maze test at different time points after pretreatment with MTX.After the Morris water maze test,the hippocampus were harvested and the expressions of C/EBP homologous protein (CHOP),cysteinyl aspartate specific proteinase 12(caspase-12) and cleave cysteinyl aspartate specific proteinase 3 (cleaved caspase-3) were detected by using Western blot.Meanwhile,cell apoptosis and pathological change of hippocampal neurons were detected by terminal dexynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay and HE staining respectively.Results In the Morris water maze test,the time in platform quadrant of rats in MTX60 group and MTX100 group was shorter than that of rats in control group during probe training [(27.30 ±3.98) s and (21.63 ±4.22) s vs (33.30 ±6.31) s,F =13.94,P ＜0.05],and the time in target quadrant of MTX100 group was shorter than that of MTX60 group (P ＜ 0.05).Compared with the control group,there were degenerated neurons in hippocampus cornu ammonis 1 (CA1) area in MTX60 group and MTX100 group.The number of TUNEL-positive cells of the hippocampus CA1 area increased significantly in MTX60 group and MTX100 group rats [(4.72 ±0.12)％ and (9.12±0.12)％ vs (1.11 ±0.49)％,F=95.272,P ＜0.05],and the TUNEL-positive cells of rats in MTX100 group were more than those of MTX60 group (P ＜ 0.05).The expressions of CHOP,caspase-12 and cleaved caspase-3 also increased compared with the control group (CHOP:2.98 ±0.31 and 4.15 ±0.61 vs 0.38 ±0.12,F =232.74,P ＜ 0.05;caspase-12:0.33 ±0.04 and 0.43 ±0.06 vs 0.14 ±0.02,F =120.70,P ＜ 0.05;cleaved caspase-3:0.35 ± 0.04 and 0.44 ± 0.06 vs 0.05 ± 0.03,F =198.64,P ＜ 0.05),and the protein expression levels of rats in MTX100 group were higher than MTX60 group (all P ＜ 0.05).Conclusions MTX can induce cognitive impairment in rats,and endoplasmic reticulum stress mediated hippocampal neurons apoptosis may play an important role in the mechanism of MTX-induced cognitive impairment in rats.

Objective To find out about the attitudes of hospital runners, academic leaders and key members of the technical force in public hospitals about structural reform in their institutions and to explore modes and methods for such reform. Methods Survey questionnaires were designed, anonymous surveys were conducted on the spot, and statistical analyses were made of the data collected. Results Surveys on 1904 people in 55 second- and third-tier hospitals indicated that 84.9% were in favor of structural reform in their institutions; 89.7% played an active part in the reform; 86.8% held that structural reform should first be carried out in large and medium-sized hospitals; 96.6% were in favor of such reform modes as government-controlled stock systems, government-owned and civilian-run hospitals, and non-governmental hospitals, with 66% of them supporting the mode of government-controlled stock systems. Conclusion The majority of hospital runners, academic leaders and key members of the technical force in public hospitals actively support structural reform in their institutions and their views with regard to the modes and methods for such reform are in accordance with the guidelines put forward by the government. Sound ideological foundations have been laid for duly promoting structural reform in public hospitals.

Objective: To determine the efficacy of 4 cervical ripening and labor induction methods in women at term. Methods:Two hundred and ten women who had clinical indications for labor induction were randomly allocated into 5 groups: mife-pristone(A) ,Diluoan(B) ,oxytocin(C), misoprostol(D)and control(E)groups. Results: The cervical Bishop score in group Awas increased to 3.39-+0.87 and higher than those in other groups(P＜0.05). The success rates of labor induction andvaginal delivery in mifepristone combined with misoprostol or oxytocin were significantly higher than those in other groups(P ＜0.05). Conclusion: Mifepristone is an effective inductive agent for cervical ripening, misoprostol and oxytocin werestrong uterine contract revulsivum. Mifepristone combined with misoprostol or oxytocin was considered as the best way of la-bor induction in term pregnancy.

Objective Drug resistance is a major problem for the successful chemotherapeutic treatment and prognosis of cervi -cal cancer .The article investigated the role of Twist on cisplatin resistance of Hela cervical cancer cells in hypoxia microenvironment and its possible mechanism to provide an experimental basis to improve the therapeutic efficacy for cervical cancer . Methods Hela Cells were divided into two groups, normal oxygen group A (no CoCl2) and hypoxia group A (addition of 150μmol/L CoCl2 medium).6 hours later, different degrees(10-3, 10-4, 10-5, 10-6, 10-7 mol/L) of cisplatin (10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 mol/L) were added into two groups .24 hours later , MTT were added to measure IC 50 and growth inhibition ratio .CoCl2 were used to mimic hypoxia environ-ment.The working concentration and the treatment duration of Cispla-tin was optimized by MTT method.The expressions of Twist and MDR1 in normal oxygen group , cisplatin group , hypoxic group B and hypoxic cisplatin group B were determined by immunofluores-cence and western blot . Results The IC50 values were 10 -5.3 mol/L and 10 -4.5 mol/L of cisplatin respectively in normal oxygen group and hypoxia group, and there was significantly difference between them (P<0.05).The optimized working concentration and work time of cisplatin was 10 -5 mol/L and 24 h.Compare to the normal oxygen group (13.08 ±2.39, 29.57 ±12.80), the expres-sions of Twist (20.81 ±2.07, 24.25 ±4.51, 33.14 ±4.24) and MDR1 (35.26 ±8.41, 60.13 ±22.32, 76.00 ±9.96) was signifi-cantly higher than those in other three groups and which were the highest in hypoxic cisplatin group (P<0.05).There were significant positive correlations among them in hypoxic group and hypoxic cisplatin group (r =0.686,P <0.05;r =0.546,P <0.05). Conclusion The hypoxia microenvironment may be related to the cisplatin resistance of Hela cervical cancer cells .The possible mechanism is hypoxia activates Twist and upregulates MDR 1 expression , resulting in cisplatin resistance of Hela cells .