Objective:To study the expession of TGF-? 2,TNF-? and its relationship with hepatitis B(HBV) infection in chronic hepatitis B,liver cirrhosis,paratumor cirrhosis and hepatocellular carcinomas(HCC).Methods:HBsAg,HBcAg,TGF-? 2 and TNF-? were detected by immunohistochemical staining among total 63 cases of liver cirrhosis,HCC,paratumor cirrhosis and normal liver tissues.Results:TGF-? 2 and TNF-? were expressed in liver cirrhosis,HCC and paratumor cirrhosis,and they showed significant difference from normal control liver( P c

Objective:To study the probable mechanism of HBV effect on cellular immune system in human body by detecting the quantity and function of CD4~+T and CD8+T cells in peripheral blood and liver tisues.Methods:1)The quantity of CD4~+T and CD8~+T cells in peripheral blood was detected by flow cytometry(FCM) in 36 patients with hepatitis B and 20 cases of controls;2)The expressions of HBsAg and HBcAg were detected by streptavidin-peroxidase immunohistochemistry(SP).50 cases of positive HBsAg and(or)HBcAg were selected as HBV(+) group,and 10 cases of negative HBsAg and HBcAg in liver tissue and blood serum were selected as HBV(-) group.The expressions of CD4,CD8,CD25 in liver tissues were detected in the two groups.Results:1)The quantity of CD4~+T cells and the ratio of CD4~+T/CD8~+T in peripheral blood of patients was significantly lower than those in controls;the quantity of CD8~+T cells was considerably higher than that in controls.(2)T lymphoid cell infiltrates in the liver were most gathered in the lobules and the portal tract.The densities of CD8~+T cells were higher in lobules and portal tracts than CD4~+T cells.The quantity of CD8+T and CD4~+T cells in the liver tissues of HBV positive group showed significant difference from the liver tissues of HBV negative group(P~b

Objective To investigate the regulatory effect of TNF ? on the expression of plasminogen activator inhibitor 2(PAI 2) and its biological significance. Methods Using immunocytochemistry(ICC), terminal deoxynucleotidyl transferase mediated end labeling (TUNEL) and ICC/TUNEL double label methods, the expression of PAI 2 in and the apoptosis of the cultured epidermal keratinocytes treated by TNF ? were detected. Results After the mouse epidermal keratinocytes were incubated with TNF ?, compared with the unshed cells, the expression of PAI 2 in shed cells and the cellular apoptosis increased obviously. The increased PAI 2 expression also appeared in the apoptotic cells, especially in larger and multi angled apoptotic cells. Conclusion In highly differentiated epidermal keratinocytes, TNF ? can enhance the expression of PAI 2 as well as apoptosis. Furthermore, according to the relation with the keratinocytes apoptosis, there are two types of PAI 2 induced by TNF ?, one of which is related to apoptosis, but the other is not.

Objective To investigate the efficacy and safety of amniotic membrane transplantation in the treatment of severe chemical burns.Methods Amniotic membrane transplantation were performed in 41 patients(41eyes)suffered with severe chemical burns,in which 26 cases were alkaline burns,15 cases were acid burns.Results 41 cases were followed up about 6-24 months,the effective rate were 92.7%,alkaline burns were 92.3%,acid burns were 93.3%.Conclusions Amniotic membrane transplantation is an effective and safe method for the treatment of chemical burns.

24 days in mm, V1:the degree of expansion in ml) had linear correlation, E1=(0.86V1?0.60) mm. Conclusion The results of the study demonstrate that CT is an accurate and reliable imaging technique in the evaluation of the degree of expansion of the injured orbit and in the prediction of the possibility of the consequent enophthalmos and its degree. It is significantly helpful to the therapeutic planning for the quantity the retroglobal filling in the rectification of the enophthalmos.

Objective To explore the efficacy and safety of tenofovir (TDF)+ lamivudine (3TC)+ efavirenz (EFV)combina-tion therapy in the treatment of HBV/HIV coinfection and provide evidence for effective management of HBV/HIV coinfec-tion.Methods All the AIDS/HIV patients treated in our hospital from February 2011 to October 2011 were screened for eligi-bility in this analysis.Twenty eligible patients with HBV/HIV coinfection were treated with the TDF/3TC/EFV triplet regi-men.The efficacy and safety of the combination therapy were evaluated.Results Of the 15 patients who had completed study period of 48 weeks,all had conversion of HBV DNA to negative,2 had HBeAg seroconversion.The liver function tests were normal.HIV-RNA was below the limit of detection in 15 cases.CD4+ increased by 139.72±3.36/μL on average.The creati-nine and lactate levels were within normal limits.No adverse events were reported.Conclusions The TDF/3TC/EFV triplet regimen is safe and effective for treatment of HBV/HIV coinfection.It is appropriate to recommend this regimen as a standard therapy for HBV/HIV coinfection.

Morus alba L. (mulberry) is a well-known deciduous tree, belonging to the genus of Morus of Moraceae famlily. Its leaves, twigs, roots (bark) and fruits are widely used in the traditional Chinese medicine. The active constituents of mulberry contained flavonoids, alkaloids, steroids, coumarins, with the significant hypoglycemic, hypolipidemic, antihypertension, anti-oxidation, anti-inflammatory, anti-bacterial, anti-tumor and immunomodulatory activities. This review summarized the research progress of the major pharmacological activity, pharmacokinetics and drug-drug interaction based on CYPs and transporters of mulberry and its active constituents.
Alkaloids ; pharmacology ; Coumarins ; pharmacology ; Flavonoids ; pharmacology ; Fruit ; chemistry ; Herb-Drug Interactions ; Humans ; Medicine, Chinese Traditional ; Morus ; chemistry ; Plant Extracts ; pharmacology ; Plant Leaves ; chemistry ; Plant Roots ; chemistry ; Steroids ; pharmacology

Objective To investigate arterial blood gas(ABG)changes induced by different intra- abdominal pressures during CO_2 pneumoperitoneum in rabbits with lung and liver impact injury under controlled hemorrhage.Methods In this study 75 rabbits were randomized into nine groups(6 rabbits in each group)according to the amount of blood loss(6 ml/kg、12 ml/kg、22 ml/kg)and intra-abdominal pressures(5 mm Hg,10 mm Hg,15 mm Hg).After model was established successsfully,respiratory rates (RR),ABG and death rates were observed at pre-pneumoperitoneum,after 0.5 hour and 2 hours under pneumoperitoneum and 0.5 hour after desufflation.Results Without pneumoperitoneum,different blood loss exerted different effect on arterial blood gas:RR,PaCO_2 increased(P

CDFI was performed in 20 patients with acute upper ureteral obstruction secondary to ureteral stone. Intrarenal flow of unilateral obstructed kidneys and contralateral nonobstructed kidneys was observed. Resistive index (RI) of intrarenal arteries was measured. The results showed that RI of intrarenal arteries in unilateral obstruted kidneys was elevated during acute upper ureteral obstruction with renal colic. The RI in contralateral nonobstructed kidneys did not change. Statistically significant differences were noticed in the groups of normal versus obstructed kidneys (P

OBJECTIVE To investigate the drug-resistance of ESBLs-producing Escherichia coli for guiding the clinical drug-using.METHODS The Kirby-Bauer agar diffusion method was used to analyze the drug resistance of 68 E.coli isolates in these three years.The ESBLs-producing strains were detected by confirmed test.RESULTS Thirty one ESBLs-producing strains were detected and the isolated rate was 54.4%;the resistant rates of these isolates were up to 50% to penicillin and three generations cephalosporin.The resistant rate to quinolone was about 70%.All of the strains were susceptible to imipenem.CONCLUSIONS More attention should be paid on the detection of ESBLs-producing E.coli and prevent the prevalence of the drug-resistant strains.