Objective To observe the application of oxycodone with the efficacy and safety of propofol anesthesia in hysteroscopic surgery. Methods 60 cases of hysteroscopic surgery in our hospital in January 2016 January 2017 were admitted during the period, according to the anesthesia were divided into study group and control group, 30 cases in each. The study group used oxycodone with propofol the control group was treated with fentanyl anesthesia with propofol anesthesia effect and safety of anesthesia. Results Before anesthesia, study group and control group, heart rate and arterial pressure comparison, no statistically significant; after anesthesia, study group and control group, heart rate and arterial pressure contrast, comparison was statistically significant. The study group and the control group of adverse reactions during and after the operation, there was statistical significance. Compared with the control group, the analgesic effect of uterine contractions after 5min, 1 h and 2 h in the contrast group was statistically significant. Conclusion The application of oxycodone analgesia with propofol in the operation is effective in hysteroscopic surgery anesthesia, intraoperative respiratory depression, little effect on the circulatory system, high safety.

Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg?L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg?L~(-1)(P

Objective To evaluate the value of CT-guided self-made positioning puncture device in radiofrequency ablation of liver tumors. Methods A total of 78 tumor foci from 64 patients with primary hepatocellular carcinoma treated with radiofrequency ablation from January 2010 to November 2014 were analyzed. Among them, 13 tumor diameters were 3.5-5.0 cm and 65 < 3.5 cm. The location and puncture of tumor lesion were performed by CT-guided positioning device, and the effect of puncture and lesion was evaluated by using tumor precise radiotherapy planning system. Radiofrequency ablation of tumor lesion was performed by tumor radiofrequency treatment system. Results The puncture error of all tumors in the space was 1-4 mm, the average puncture error was 2.9 mm, the complete ablation rate of tumor lesions was 93.59%(73/78), and there was no serious complication in the course of treatment. After treatment, the alpha-fetoprotein and liver function were significantly improved, and the differences were statistically significant (both P<0.05). At 1 month after surgery, CT found that no tumor and ablation was enhanced, at 3 months after surgery, CT showed that the arterial phase of all the tumor lesions did not strengthen, all of them were low-density lesions, no ablation recurred. The recurrence rates of 1, 2, 3, and 4 years after treatment were 17.9 %, 29.9 %, 45.9 % and 55.0 %. Conclusion CT-guided radiofrequency ablation with self-made puncture device is effective in the diagnosis of primary liver cancer, ablation is complete and safe.

The specific forms and contents of narrative nursing clinical practice were analyzed. On the basis of a brief review of the concept and origin of narrative nursing and the significance of it, this paper focuses on the field, methods, forms and evaluation methods of clinical practice of narrative nursing. The study found that through the literature review, the value of narrative medicine has been fully affirmed, but how to implement theory to a real clinical nursing practice environment has not yet been reported more. Therefore, exploring the narrative nursing in the clinical workplace, rich forms, contents and methods of clinical practice can provide new methods and new ideas for nursing humanistic care and clinical practice, and has certain social significance, academic significance, individual significance and application prospect.

Objective To understand the characteristics of malaria endemic situation in Dongtai City from 1999 to 2013. Methods The data of diagnosed malaria cases and related information of prevention and treatment were collected and analyzed with the epidemiological method. Results From 1999 to 2013 there were 27 malaria cases in total and among which 2 cases were local-infected with Plasmodium vivax 10 cases were imported P. vivax infections 13 cases were imported P. falciparum infections and 1 case was a mixed infections P. falciparum P. vivax and P. ovale . The imported cases accounted for 92.59%and local-infected cases accounted for 7.41%. There were no local-infected cases after 2003 and all the cases were overseas-im-ported. The main first-diagnosis points were town hospitals and village health stations accounting for 33.33%and 33.33%re-spectively. Totally 62.96%of cases visited the clinic in 1 day after the attacking the median of time-interval from the attacking to visiting the clinic was 1 day the median of time-interval from the attacking to confirmed diagnosis was 3 days and the max time from the attacking to confirmed diagnosis was 33 days. Conclusion The malaria cases are mainly imported cases in Dong-tai City from 1999 to 2013 and therefore the key of eliminating malaria should be the monitoring of the retuned exporting la-bors.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

The taxol s effect of inducing apoptosis is mainly related to its ability to promote tubulin polymerization and prevent tubulin chain depolarization. Drug resistance is the main restriction factor for clinical usage of taxol. Some basic and clinical studies support that poor response to taxol and poor prognosis are related to overexpression of β tubulin Ⅲ.

Objective To study the changes of ICAM-1 expression in transplanted lung at the early stage after rat pulmonary transplantation.Methods The expression level of ICAM-1 protein and its mRNA in rat transplanted lung after 4 h of ventilation and reperfussion were detected by using immunohisto chemical method(SABC method)and semiquantitative reverse transcriptase polymerase reaction method in the experimental group(n=6).When donor lungs had been flushed with low potassium solution(LPDS) and preserved in 4.C LPDS for 12 h,left orthotopic pulmonary transplantation was performed.In control group (n=6), left pulmonary artery,pulmonary vein and bronchi were fully freed to be naked.Immunohistochemical test results were recorded in negative(-),suspicion(±),faint positive(+),positive(++)and intensive positive(+++).PCR products were separated by agrose gels and the density of the bands were determined by density scanning.Results The stained color of alveolar epithelial cells and pulmonary vascular endothdial cells in the transplanted lung was significantly more intensive in experimental group than in control group(P＜0.01) and the relative density values(ICAM-1/βactin)were also significandy higher in experimental group(0.837±0.044) than in control group (0.442±0.037),P＜0.01. Conclusions ICAM-1 expression in the transplanted lung was up-regulated in the early stage after pulmonary transplantation,which was related with the enhancement of ICAM-1 mRNA.

This study reviews the researches on self-handicapping in about more than 20 years. It summarizes the practical researches of self-handicapping from 7 aspects: (1)manifestations and types of self-handicapping behaviors;(2)evaluative conditions that typically elicit self-handicapping;(3)motives underlying self-handicapping; (4)factors influencing on self-handicapping; (5)consequences of self-handicapping; (6) measurements of self-handicapping. On this basis, it raises some questions in self-handicappping researches and points out the future direction.