Objective: To observe the effects of ginsenoside Rg_(1) , chitosan and TGF-?_(1) on the proliferation and differentiation of human periodontal ligament cells( PDLC). Methods:Human periodontal ligament cells were isolated and cultured. The effects of ginsenoside Rg_(1)(0.01 ?mol/L), chitosan((0.05 g/L,)0.1 g/L, 0.2 g/L) and TGF-?_(1)(0.5 ?g/L, 1 ?g/L, 2 ?g/L) on the proliferative ability of human PDLCs were evaluated with MTT method. The alkaline phosphatase activities of human PDLCs were measured with spectrophotometric assay. The secretion of osteocalcin of human PDLCs were measured with radioimmunological method and the apotosis rates of human PDLCs were assayed with flow cytometry with PI staining method. Results: ①Comparing with the control group, the proliferative ability of human PDLCs in ginsenoside Rg_(1)(0.01 ?mol/L)group ,Chi(0.05 g/L, 0.1 g/L, 0.2 g/L) groups and TGF-?_(1 )((0.5 ?g/L), 1 ?g/L, 2 ?g/L) groups on day 3,5,7 were considerably increased (P

Objective To explore the effect of fluticasone propionate nasal spray(Fu Shu-liang) in improving children's symptoms of allergic rhinitis and recurrent asthma.Methods 100 children with allergic rhinitis and asthma syndrome were randomly divided into control group and experimental group.The control group was treated with loratadine.The experimental group was treated with water-soluble auxiliary Fu Shu-liang nasal spay on the basis of the control group.The treatment effects were observed.Results The total effective rate of the observation group was 94％,which was significantly higher than 76％ of the control group (x2 =6.35,P ＜ 0.05).After 10-12 weeks,the rhinitis symptom score and asthma symptom score between the two groups had statistically significant differences (t =2.47,2.64,3.41 ; 2.30,3.17,2.47,all P ＜ 0.05).The incidence rate of adverse reactions such as nasal dryness,epistaxis between the two groups had statistically significant differences (x2 =7.11,7.53,all P ＜ 0.05).Conclusion Fu Shu-liang(Eph propionate and fluticasone propionate nasal spray) has good effect in improving symptoms of children with allergic rhinitis and asthma,it is worthy of clinical vigorously promoted.

Objective To explore the difference of serum and pleural effusion CEA ,CA125 and CA199 levels in benign and ma‐lignant pleural effusions to accumulate the practical experience for clinical diagnosis .Methods 125 patients with pleural effusion in this hospital from February 2010 to March 2014 were performed the retrospective analysis and divided into the benign group(49 ca‐ses) and malignant group(76 cases) according to the nature of the fluids .The etiology composition ,pleural effusion and serum tumor markers levels were recorded in the two groups and at the same time the diagnosis efficiency of the tumor markers in pleural effusion was calculated .Results The specificity of the combined detection of CEA ,CA125 and CA199 for diagnosing benign and malignant pleural effusion was 90 .6% ,the sensitivity was 95 .1% ,the positive predictive value was 90 .2% ,negative predictive val‐ue was 95 .3% and accuracy was 92 .7% ,which were higher than those of the single index detection and any 2‐marker combined de‐tection .Conclusion The combined diagnosis of CEA ,CA125 and CA199 in pleural effusion conduces to the assisted diagnosis of be‐nign and malignant pleural effusion .

Objective To analyze the pathophysiological variation in patients with penetrating cardiac trauma (PCT) and discuss the classified treatment strategies.Methods A retrospective analysis was conducted on pathogenetic condition,medical interventions and other clinical information of 133 PCT patients.The patients were divided into four groups in accordance with their symptom classification on arrival at emergency department,including sub-clinic type,cardiac tamponade type,hemorrhagic shock type and agonal type.Meanwhile,the patients were classified into three groups according to their pathogenesis phase,including sub-clinical phase,clinical phase and agonal phase.Development characteristic of patients' condition in each group was investigated in each time point by survival analysis.Impacts of medical interventions on prognosis of those patients were analyzed,such as pericardial space exploration,emergency room thoracotomy (ERT),operating room thoracotomy (ORT).Results Some patients at sub-clinical phase were aggravated into clinical phase or agonal phase,as well as some patients at clinical phase were aggravated into agonal phase during in-hospital treatment.There were significance differences of posttraumatic suvival course among the four groups,namely sub-clinical type,cardiac tamponade type,hemorrhagic shock type and agonal type (P ＜ 0.01).The differences of posttraumatic survival course were also significant among the three groups,namely sub-clinical phase,clinical phase and agonal phase (P ＜ 0.01).Conclusion Clinical symptom classification (or pathogenesis phase) of PCT may not be always unchangeable,thus it is recommended that PCT patients should be treated based on their clinical symptom classification or patbogenesis phase at consultation.

Objective: To investigate the root canal configuration and the incidence and location of the isthmus in extracted maxillary second premolar with single root. Methods: 60 maxillary second premolars were included in the study. 1 mm root was cut from the apical end of root. The transverse section was stained with methylene blue dye and viewed with a surgical operating microscope. 1 mm increment sections were made and stained and observed by the same way from apex to 6 mm. The number of canals as well as the type of canal isthmuses were recorded. Results: 41.67% maxillary second premolar with single root had two canals. Canal isthmus happened more frequently in the apical 4 mm levels. Conclusion: The incidence of isthmus in the maxillary second premolar was highest in the apical 3-to 5-mm level. Canal morphology in the apical 1-to 6-mm is complicated.

0.05).Micro-suture materials has produced milder tissue reaction than silk during all experimental periods(P

To evaluate the value of detection of DNA aneuploidy in exfoliated airway epithelia cells of sputum specimens by the automated image cytometry for the identification of lung cancer, 100 patients were divided into patient group (50 patients with lung cancer) and control group (30 patients with tuberculosis and 20 healthy people). Sputum was obtained for the quantitative analysis of DNA content of exfoliated airway epithelial cells with the automated image cytometry, together with the examinations of brush cytology and conventional sputum cytology. Our results showed that DNA aneuploidy (DI>2.5 or 5c) was found in 20 out of 50 sputum samples of lung cancer, 1 out of 30 sputum samples from tuberculosis patients, and none of 20 sputum samples from healthy people. The positive rates of conventional sputum cytology and brush cytology were 16% and 32%, which was lower than that of DNA aneuploidy detection by the automated image cytometry (P<0.01, P>0.05). Our study showed-that automated image cytometry, which uses DNA aneuploidy as a marker for tumor, can detect the malignant cells in sputum samples of lung cancer and it is a sensitive and specific method serving as a complement for the diagnosis of lung cancer.
*Aneuploidy ; DNA, Neoplasm/*analysis ; Image Cytometry/methods ; Image Processing, Computer-Assisted ; Lung Neoplasms/genetics ; Lung Neoplasms/*pathology ; Sensitivity and Specificity ; Sputum/*cytology

Objective To explore the cause and epidemiologic characteristics of Keshan disease.Methods The cases observation method,epidemiologic methods,fungus-mycotoxin methods and the analytic chemistry methods were used in our studies.Results From the year 1973-1975,in serious endemic areas,fifteen families and one disease outbreak village were investigated,it was found that all patients and his family members had eaten a vast amount of mould millet or maize 4-12 months before onset of the disease; the millet samples collected from endemic areas were cultured and they werc found to be polluted mainly by fungus(20％ of all),the toxin was citreoviridin(CIT).The cause factor of Keshan disease entered into human body through grain but not drinking water,and the transmitting effect was not different with different grains,millet and maize transmit but wheat did not;according to data of endemic disease inspection,after 1990 no new cases of Keshan disease had been reported in the country,at the same time the CIT content in grain produced in endemic areas did not reduced so quickly.However,after 1983,the reform of agriculture promoted significantly the hygienic quality of grain which was considered to be a specific cause for the decreasing of Keshan disease threatening; Keshan disease was distributed on the narrow-long areas from Yunnan till Heilongjiang.These areas were the connection parts,between the cold west north high lands and the warm-moist east-south flatlands,a narrow-long region.The year mean temperature and humidity in endemic areas might be suitable for growing of fungus and production of its toxin ; of animal experiment,rats were feed with CIT contaminated grain every day 4 mg/kg(body weight) for 4-6 weeks,the necrosis of myocardium and cell mitochondria swelling,proliferation and damage were found,just like what had been seen in died cases of Keshan disease.Conclusions The cause of Keshan disease is CIT toxin,the endemic conditions are geographic climate suitable for growing of fungus and production of its toxin and bad hygienic situation of grain storage.Some discussion about the prevention mcthod was proceeding.

The accessibility of drugs is the fundamental condition for achieving the universal health care, and price is the important factor of accessibility.It is essential to have a national price negotiations mechanism for the patented drugs and for exclusive production of drugs.As for the regular works, the national price negotiations mechanism should be ruled by law, while rules and regulations should be formulated accordingly.This paper discusses the specific content and the clarification of the legislative principle of legal framework for the negotiations, main roles, and selection of objects, procedure, results and application of the negotiations.It also analyses the existing problems are analyzed, and put forward the future legislation and corresponding rules.

In critically ill patients,evoked potential(EP) testing is an important tool for measuring neurologic function,signal transmission,and secondary processing of sensory information in real time.EP potential measures conduction along the peripheral and central sensory pathways with longer-latency potentials representing more complex thalamocortical and intracortical processing.In critically ill patients with limited neurologic exams,EP provides a window into brain function and the potential for recovery of consciousness.Brainstem auditory EP and cortical event-related potentials are most commonly used in ICU.The primary indications for EP in critically ill patients are prognostication in anoxic-ischemic or traumatic coma,monitoring for neurologic improvement or decline,and confirmation of brain death.Historically,EP has been regarded as a negative prognostic test,that is,the absence of cortical potentials are associated with poor outcomes while the presence cortical potentials are prognostically indeterminate.In recent studies,the presence of middle-latency and long-latency potentials as well as the amplitude of cortical potentials is more specific for good outcomes.Event-related potentials,particularly mismatch negativity of complex auditory patterns,is emerging as an important positive prognostic test in patients under comatose.In infants,other than anoxic-ischemic or traumatic brain injury,brainstem auditory is also widely used in premature baby,bilirubin encephalopathy and detection of possible hearing loss.