1.Use of Polymerase Chain Reaction and Serum Antibodies for Diagnosis of Enterohemorrhagic Escherichia coli.
Journal of the Korean Society for Microbiology 1998;33(1):99-110
Fecal isolates of Escherichia coli which were collected from diarrheal patients and HUS patient in Pusan National University Hospital between 1990 and 1996, were serotyped and analyzed for plasmid DNA profile, biotype, HEp-2 cell adherence ability, reactivity to eae probe and for production of verotoxins (VT). In order to ease the diagnosis of EHEC infection, a LPS- based solid phase enzyme linked immunosorbent assay was utilized to detect serological diagnosis of EHEC infection. The following results were obtained. Among 150 EPEC isolates and HUS patient's stool, 7 EHEC were found. The 7 EHEC belonged to 5 different serotypes 0157:H7, 0143:H-, 0166:H-, 0128:H2, 026:H-, and 0111:H 21 previously associated with human haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). Biochemical cheracteristic analysis indicated 7 strains were biotype 1 and was found to have siderophilins suggesting their advantagous growth in vivo. For plasmid profiles, all strains had 60 MDa plasmid and several smaller sizes of plasmids. Three strains of Escherichia coli serotype 0157:H7, 0128:H2, and 026:H- showed one pattern of adherence in the HEp-2 cell assay namely, localized adherence and were positive for eae probe when tested by colony blot hybridization assay. PCR using specific primers for VT1, VT2 was tested, and all 7 strains carried VT1 gene only. PCR products of 130-bp (VT1) and 346-bp (VT2) were successfully amplified simultaneously in a single reaction. The multiplex PCR method can be used to specifically identify EHEC. The serum obtained from HUS patient of enterohemorrhagic E. coli were analyzed for rises in titer of intibody to somatic 02, 026, 0111, 0128, 0143, 0145, 0157, and 0165. Although response to the somatic 0 correlated significantly with response to the 026 rises of antibody titer to somatic 0 in acute stage of disease and anti-VT had not so many relation to that of VT. These results suggest that ELISA can be used to detect somatic 0 in serum and it is a useful method to diagnose the infection caused by EHEC rapidly.
Antibodies*
;
Busan
;
Colitis
;
Diagnosis*
;
DNA
;
Enterohemorrhagic Escherichia coli*
;
Enteropathogenic Escherichia coli
;
Enzyme-Linked Immunosorbent Assay
;
Escherichia coli
;
Humans
;
Multiplex Polymerase Chain Reaction
;
Plasmids
;
Polymerase Chain Reaction*
;
Shiga Toxins
;
Transferrin
2.Comparison of Polymerase Chain Reaction and DNA Hybridization for Detection of the Cholera Toxin Operon of Vibrio cholerae.
Yung Bu KIM ; Yang Hyo OH ; Min Jung KIM
Journal of the Korean Society for Microbiology 1998;33(6):547-555
Cholera enterotoxin (CT) is a major virulence determinant of Vibrio cholerae 01. CI' is known to be the major virulence factor of Vibrio cholerae 01 and in accordance with the recent report showing which V. cholerae non-01 has ctx gene, we performed the molecular genetic study for the detection of ctx gene related to the production of CT at the subject Vibrio spp. except for V. cholerae non-01 and V. cholerae non-01 stock cultured in the laboratory of microbiology, College of Medicine, Pusan National University and the Vibrio spp. isolated from the marine products of Pusan General Fish Market and the sea water, and then its results are as follows: 1. PCR for the detection of ctx gene at the subject of V. cholerae 01:61H-151 having the ctx gene of which the denaturation is 1 rninute at 95'C, annealing to 1min, 30 sec at 60'C, the extension to be 1min. 30 sec at 72'C and 30 or 40 cycles. ctx gene was detected from 4 strains of V. cholera non-01 derived from the environment isolates. 2. Adjusting the quantity of chromosomal DNA used as template DNA to be from 0.1 pg to 1 ng, in order to know the PCR conditions for the effective search of ctx gene, and the detection limit of the system was 10 pg of chromosomal DNA. 3. The broth culture was used for template DNA, ctx gene of 302 bp was detected from 4 V. cholerae non-01, as in the case of chromosomal DNA, and the cell number was possible to be detected to 3 * 10.4. We attempted the confirmation of ctx gene through Southern blot hybridization, labeling with P and then it was confirmed only from 4 V. cholerae non-01 as like PCR results. 5. As the result of the sensitivity of PCR and Southern blot hybridization, it was shown to be possible which 10 pg was detected in case of chromosomal DNA and in case of cultured broth, the cell number was detected until 10 at PCR and Southern blot hybridization, and thus it was examed which its sensitivity was same.
Blotting, Southern
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Busan
;
Cell Count
;
Cholera Toxin*
;
Cholera*
;
DNA*
;
Enterotoxins
;
Limit of Detection
;
Molecular Biology
;
Operon*
;
Polymerase Chain Reaction*
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Seawater
;
Vibrio cholerae*
;
Vibrio*
;
Virulence
3.The immunological characteristics of adhesin in escherichia coli.
Yang Hyo OH ; Yung Bu KIM ; Gui Jeon CHOI ; Mi Gyeong KIM ; Min Jung KIM
Korean Journal of Immunology 1993;15(1):1-9
No abstract available.
Escherichia coli*
;
Escherichia*
4.Modification of Immune Response by Cold Stress in Mice.
Young Min PARK ; Yang Hyo OH ; Young Ran SEONG ; Jung Cheol LEE ; Seol Hyang BAEK
Korean Journal of Immunology 1997;19(4):437-446
No abstract available.
Animals
;
Interleukin-2
;
Mice*
5.Amplification and purification of the Tn5 encoded transposase protein by simple in vivo transposition.
Byung Tae PARK ; Bong Hoon KIM ; Min Ho JEONG ; Yang Hyo OH
Journal of the Korean Society for Microbiology 1991;26(2):195-204
No abstract available.
Transposases*
6.Detection of Multidrug Resistant Patterns and Associated - genes of Methicillin Resistant Staphylococcus aureus ( MRSA ) Isolated from Clinical Specimens.
Eun Gyoung LIM ; Young Hee KIM ; Ji Yung MUN ; Yung Bu KIM ; Yang Hyo OH
Journal of the Korean Society for Microbiology 2000;35(5):356-356
No Abstract Available.
Methicillin Resistance*
;
Methicillin*
;
Methicillin-Resistant Staphylococcus aureus*
;
Staphylococcus aureus*
;
Staphylococcus*
7.Virulence Factors and Genotyping of Vibrio parahaemolyticus.
Eun Gyoung LIM ; Young Hee KIM ; Ji Yung MUN ; Yang Hyo OH ; Yung Bu KIM
Journal of the Korean Society for Microbiology 2000;35(5):355-355
No Abstract Available.
Vibrio parahaemolyticus*
;
Vibrio*
;
Virulence Factors*
;
Virulence*
8.Detection of Multidrug Resistant Patterns and Associated - genes of Methicillin Resistant Staphylococcus aureus ( MRSA ) Isolated from Clinical Specimens.
Eun Gyoung LIM ; Young Hee KIM ; Ji Yung MUN ; Yung Bu KIM ; Yang Hyo OH
Journal of the Korean Society for Microbiology 2000;35(5):356-356
No Abstract Available.
Methicillin Resistance*
;
Methicillin*
;
Methicillin-Resistant Staphylococcus aureus*
;
Staphylococcus aureus*
;
Staphylococcus*
9.Virulence Factors and Genotyping of Vibrio parahaemolyticus.
Eun Gyoung LIM ; Young Hee KIM ; Ji Yung MUN ; Yang Hyo OH ; Yung Bu KIM
Journal of the Korean Society for Microbiology 2000;35(5):355-355
No Abstract Available.
Vibrio parahaemolyticus*
;
Vibrio*
;
Virulence Factors*
;
Virulence*
10.Identification and characterization of the fimbrial adhesin and gene product that regulates the expression of fimbriae in escherichia coli.
Yang Hyo OH ; Yung Bu KIM ; Gui Jeon CHOI ; Mi Gyeong KIM ; Min Jung KIM
Journal of the Korean Society for Microbiology 1992;27(5):391-405
No abstract available.
Escherichia coli*
;
Escherichia*