Objective To study the protective effect of leuprorelin particle on ovarian reserve function applied before the laparoscopic cystectomy of ovarian endometriosis.Methods 106 patients with endometriosis treated from January 2015 to December 2015 in our hospital were selected.The subjects were divided into two groups according to the random number table, 53 cases of each group.The control group adopted the laparoscopic surgery;the observation group took the leuprorelin particle before the laparoscopic surgery.The operation condition, ovarian hormone and function were observed. Results The operation time and bleeding amount in observation group was better than control group ( P <0.05 ) .After surgery, the exhaust time between two groups had no significantly different.After surgery, the level of follicle stimulating hormone (FSH) in observation group was lower than control group; the level of estradiol (E2) in observation group was higher than control group (P <0.05); the level of luteinizing hormone (LH) between two groups had no significantly different.After surgery, the ovarian volume and antral follicle count ( AFC) in observation group was better than control group (P<0.05).Conclusion Before the laparoscopic cystectomy of ovarian endometriosis, the leuprorelin particle protects the ovarian function and morphology and has less impact in the level of hormone.

There is a great controversy over the application of corticosteroids in preventing and treating bronchopulmonary dysplasia(BPD).Prenatal use of corticosteroids may reduce the occurrence of BPD.Intravenous corticosteroids within 1 week after birth can reduce the incidence of BPD.However,in light of the short and long-term adverse reactions,it is not recommended.For premature infants with BPD in high risk,clinicians should weigh the pros and cons and may apply a small dose,short course of corticosteroids for intervention in 1 week after birth.Early inhalation or instilling of corticosteroids may be an effective and safe way to reduce the occurrence of BPD.

Objective To detect the expression of HSPA2 mRNA and protein in pancreatic cancer and corresponding adjacent nontumorous tissues, and investigate the expression of HSPA2 protein in pancreatic cancer and its association with clinicopathological characteristics.Methods Western blot and qPCR (real-time quantitative PCR) was used to evaluate HSPA2 expression in pancreatic cancer and corresponding adjacent nontumorous tissues.Immunohistochemistry was used to investigate the HSPA2 expression in pancreatic cancer and its association with clinicopathological characteristics.Results Both the mRNA and protein levels of HSPA2 were significant higher in pancreatic cancer tissues than those in their paired adjacent nontumorous tissues.High HSPA2 expression was detected in 68.8% (55/80) of pancreatic cancer tissues.HSPA2 protein expression was significantly associated with tumor differentiation, vascular invasion, and status of metastasis (P=0.011,0.005,0.012).Conclusion HSPA2 expression is elevated in pancreatic cancer, and positively correlated the process of invasion, metastasis and progression of pancreatic cancer.

Objective To observe the effect of ambroxol hydrochloride combined with intervention in chronic obstructive pulmonary disease and evaluate its clinical effect. Methods A total of 88 patients with chronic obstructive pulmonary disease (COPD) who were treated in Changxing Hospital of Zhejiang Province from March 2014 to April 2017 were randomly divided into study group and control group. The control group was treated with ambroxol, and the study group was given comprehensive nursing intervention on the basis of control group. The clinical treatment of the two groups was observed.Results After treatment, the total effective rate of the treatment group was 95.45％ (42/44), the control group was 77.27％(34/44), the difference was statistically significant (P<0.05); there were no significant differences in blood gas analysis (PaO2, PEF) and lung function (MVV, Fres, Zrs, R5-R20, Cdyn) before treatment, after treatment, the improvement of the indicators in the study group was significantly better than the reference group (P<0.05). Conclusion Ambroxol combined with comprehensive nursing intervention for chronic obstructive pulmonary disease, can effectively improve the clinical treatment effect, help to improve lung function, promote the recovery of the disease, with clinical application value.

This paper summarizes researches about research-oriented hospital,and proposed the concept of research hospitals.The connotation construction of a research hospital is discussed in terms of the quality of medical care,personnel training,scientific research and management capacity.

Objective This study is to examine the effect of different breathing patterns (abdominal respiration,natural respiration)on skin temperature(TEMP),skin conductivity(SC) and heart rate variability (HRV) in college students.Methods We use single blind trial in the study,and randomly select thirty students,including fourteen male and sixteen female,aged 19.6±0.77 years,and then randomly divide them into two groups:the intervention group (abdominal respiration group,6 males and 9 females) and the control group(natural respiration group,8 males and 7 females).In the study three phases are included:baseline phase(five minutes),test phase(fifteen minutes),recovery phase(five minutes).Data of above indicators was collected at 3-5 min,14.5-19.5 min,and 23-25 min,respectively,during the study.Results (1) There are no significant difierences of all three indicators between the two groups in the baseline phase(P<0.05).(2)The SC of the test phase in the intervention group is lower than in the control group (P=0.05),while the TEMP and HRV of the two groups show no significant differences(P>0.05).(3)There are no significant differences of TEMP and the HRV in the recovery phase between the two groups(P>0.05),except the SC (P<0.05).Conclusion No significant difference is observed between the influences of abdominal and natural respiration on the emotion-related biological indicators.

Objective: To improve the safety of anesthesia of the neonates at high risk during surgical operation. 　Methods:Before the operation, Anisodamine, Ketamine and Vecuronium bromide were injected intravenously with dosage of 0.06mg, 2mg*kg-1 and 0.08-0.1mg*kg-1, respectively. Tracheal cannula or topical anesthesia using 1% of lidocaine hydrochloride was performed. The tracheal cannulation was performed after administration of sodium hydroxybutyrate and Ketamine by injection with dosages of 50-80 mg*kg-1 and 2mg*kg-1 body weight. Anesthesia apparatus and combinative anesthesia were used. The concentrations of SpO2 were monitored during the operation. A dosage of 1-2mg*kg-1 body weight was used for resuscination of the patients after the operation. 　Results: A total of 100 cases of operation were performed using the regime of anesthetic intervention described above. A 100% of success rate was achieved and no death occurred during the operation. 　Conclusion:Significant improvement of safety in anesthesia of the neonates at high risk during surgical operation can be achieved with sufficient preoperational preparation, adequate monitoring during the operation and rational use of anesthetics as well as restrict selection of suitable anesthetical interventions.

CCN1 is a novel extracellular matrix-associated signaling protein which possesses 381 amino-acid residues to compose 4 distinct structural modules with 38 conserved cysteine residues.This protein has a variety of properties in cardiovascular system,affecting the cellular behaviors such as differentiation,proliferation and migration of vascular endothelial cells,smooth muscle cells and cardiac myocytes,that suggested an essential roles of CCN1 in angiogensis,vascular injury,cardiac development and myocardial infarction.

According to the anatomic classification,refractive surgery is divided into keratorefractive surgery,phakic refractive surgery and scleral refractive surgery.Most procedures for the correction of ametropia are performed on the cornea,with laser-assisted in situ keratomileusis(LASIK) being the most common one.LASIK has shown its limitations,however.For the correction of higher myopic and hyperopic refractive error,the procedure has considerable undesired effects,such as loss of contrast,glare,and iatrogenic keratectasia.Therefore,a variety of intraocular lens,known as phakic refractive surgery,have been designed to achieve full correction of high myopia and hyperopia without loss of accommodation.Phakic refractive surgery is different from kerato-or scleral refractive surgery in that it involves phakic intraocular lens,non-phakic intraocular lens and clear lens extraction +introcular lens.The author will outline recent progress in phakic intraocular lens and provide unifying concepts that are accepted by ophthalmologists.

BACKGROUND:Wear particles factually serve as a medium carrying extensive information on the wear process involved in hip implants.Moreover,it has been demonstrated that wear particles contribute to the failure of Charnley total hip replacement.OBJECTIVE:To investigate the morphological characteristics of wear particles generated by couples of ultrahigh molecular weight polyethylene(UHMWPE) cups and Al2O3 femoral heads,mounted in a simulator in the presence of distilled water and explore the wear mechanism of UHMWPE cup.DESIGN:Wear tests were performed on a ball-in-socket hip simulator.SETTING:Department of Mechanical Engineering,Xuzhou Institute of Technology.MATERIALS:The molecular weight of UHMWPE selected as cup was 3 000 000.Its density was 0.935 g/cm3,hardness 30 HB,fracture strength 35 MPa,and water absorption 450 MPa,and tenacity 7 MPaM1/2.METHODS:The experiment was performed in the laboratory of tribology,China University of Mining and Technology from December 2003 to July 2005.Hip simulator was used to assess the tribological performance of Al2O3-UHMWPE at room temperature,100 r/min,784 N load for 1.5?106 cycles.Distilled water was used as the lubricant.Wear of UHMWPE acetabulum was measured every 3?105 cycles by taking out the acetabulum from simulator,cleaning in distilled water for 3 minutes in an ultrasonic bath,putting in an oven for 40 minutes at 80 ℃,and cooling off in a desiccator.A BT211D electronic balance(reciprocal sensibility,0.01 mg) was used to measure the weight loss of UHMWPE acetabula according to the reference sample method to calculate the wear.A sample(20 mL) of distilled water lubricant used in test was obtained when the simulator was stopped for gravimetric analysis.Morphology of wear particles in samples and surface of worn UHMWPE acetabula were observed by using scanning electron microscope(SEM).The components of wear particles were determined by X-ray.Detailed measurements of the wear particles distribution were conducted by LS100(R) Laser Particle Size Analyzer(including LS SVM Plus).MAIN OUTCOME MEASURES:Morphology and size distribution of wear particles;Wear mechanism and wear rate of UHMWPE acetabula.RESULTS:The wear particles showed a wide range of particle sizes,varying from 0.3 to 180 ?m,most of which ranged from 5 to 20 ?m.More than 99% of wear particles were UHMWPE particles,which were classified into five groups in terms of their morphological characteristics:spherical particle,tearing particle,lamellar particle,fatigued flaking and rod-shaped particle.Lamellar or cube was the predominant form of Al2O3 ceramic particles with flat surface structure and upright edges.The worn surfaces of UHMWPE cup showed evidence of multidirectional scratching,adhesive and micro-contact fatigue wear,and plastic deformation.CONCLUSION:①The dominant wear mechanisms acting in the UHMWPE cup are abrasive wear,adhesive wear,microcosmic fatigue wear and flaking associated with plastic deformation.②The size of particles accumulating mostly is tiny among the particle groups.