1.Viability of Cultured Human Keratinocyte and Melanocyte after UVB Exposure.
Korean Journal of Dermatology 1997;35(2):258-265
BACKGROUND: Each kind of human cell has its own characteristic morphological and functional property. In the skin, epidermal cells, including keratinocyte and melanocyte, also have their own functional characteristics. Thus, it is expected that there are some different responses to external stimuli, such as ionizing radiatio,, free radicals, and cytokines between these cells. OBJECTIVE AND METHODS: To im estigate whether there are different effects of UV light on the viability of cultured human ker tinocytes and rnelanocytes. Cultured human keratinocytes and melanocytes are irradiated by UVB at 5, 25, 50, and 100mJ/cm, and examined by Methylthiazole tetrazollium assay at 0, 1, 3, 6, 24, 48, and 72 hours after UVB exposure. RESULTS: 1. The effects on viability according to the doses of UVB are as follows: 1) In the keratinocytes, the viability was increased in most of the UVB exposure groups within 24 hours after UVB exposure, and was significantly increased at 25, 50, and 100mJ/cm of UVB at 3 hours after UVB exposur.(p<0.05). However, the viability was significantly decreased at relatively high doses of UVB (50, 100mJ/cm) from 48 hours after UVB exposure (p<0.05). 2) In the melanocytes, the viability was decreased in all of the UVB exposure groups within 3 hours, and was significantly decreased in all of the UVB exposure groups at, 1 hour after UVB exposure (p<0.05). The viability was increased from 6 to 24 hours, which was significantly decreased at 100mJ/cm of UVB from 48 hours after UVB exposure (p<0.05). 2. The effects on viability according to the time after UVB exposure at the same dose of UUB In both cells, the viability was increased as time went by. The slopes of the viability curve gradually decreased according to the increment of UVB doses. CONCLUSION: The viability of keratinocyte was decreased at 50mJ/cm of UVB which melanocyte did not show decrease. Melanocyte was more easily damaged than keratinocyte in relatively earlier time period after UVB exposure. These results suggest that the change of viability in cultured keratinocyte and melanocyte after UVB exposure at the dose of less than 100mJ/cm is related to the time course after UVB exposure as well as to the UVB dose.
Cytokines
;
Free Radicals
;
Humans*
;
Keratinocytes*
;
Melanocytes*
;
Skin
;
Ultraviolet Rays
2.Microscopic Findings of Cultured Human Melanocytes from a Vitiligo Subject.
Korean Journal of Dermatology 1997;35(3):571-574
Vitiligo is an acquired systemic disease of the skin characterized by circumscribed patches of complete pigment loss due to destruction of melanocytes. A 28-year old male patient presented with generalized depigmented patchs. We performed microscopic studies of cultured melanocytes from this patient and compared them with those of normal neonatal foreskin. Phase contrast microscopic findings revealed no difference between the two groups of melanocytes, but transmission electron microscopic findings showed dilated circular rough endoplasmic reticulum in cultured melanocytes from our vitiligo patient. We could observe the innate cellular structural aberration of melanocytes from the vitiligo subject.
Adult
;
Endoplasmic Reticulum, Rough
;
Foreskin
;
Humans*
;
Male
;
Melanocytes*
;
Skin
;
Vitiligo*
3.A Case of Buschke-Lowenstein Tumor in Renal Transplant Recipient.
Kwang Hoon LEE ; Ju Hee LEE ; Won Soon CHUNG ; Kee Yang CHUNG ; Kwang Hoon LEE
Annals of Dermatology 2002;14(3):164-167
Buschke-Lowenstein tumor is a rare disease in the category of designated as verrucous carcinoma characterized by its invasive downward penetration of underlying tissues in the perineum and perianal regions. Viruses, unclean sanitation and cytotoxic immune reaction have been proposed as the etiology of the tumor. However, among all the causes, recent studies have emphasized on the associaton of the tumor and human papilloma virus (HPV). Expecially, HPV also has been discovered in several cutaneous and anogenital lesions of solid organ transplant recipients. We herein report a case of Buschke-Lowenstein tumor in a renal transplant recipient with HPV 6 and 16 coinfection proved by HPV genotyping of DNA extracted from the biopsy specimen of the tumor.
Biopsy
;
Buschke-Lowenstein Tumor*
;
Carcinoma, Verrucous
;
Coinfection
;
DNA
;
Human papillomavirus 6
;
Humans
;
Papilloma
;
Perineum
;
Rare Diseases
;
Sanitation
;
Transplantation*
;
Transplants
4.The Role of Partial Cystectomy for Transitional Cell Carcinoma of the Urinary Bladder.
Do Hoon YANG ; Sung Joo HONG ; Min Sung LEE
Korean Journal of Urology 2000;41(11):1316-1322
No abstract available.
Carcinoma, Transitional Cell*
;
Cystectomy*
;
Urinary Bladder*
5.Resurfacing of the hand using free temporoparietal fascial flap.
Young Seob LEE ; Yang Woo KIM ; Byung Hoon RYU
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(2):252-257
No abstract available.
Hand*
6.Sleep Problems in Autism Spectrum Disorder.
Young Hui YANG ; Ji Hoon KIM ; Jin Seong LEE
Sleep Medicine and Psychophysiology 2013;20(2):53-58
Autism Spectrum Disorder (ASD) is characterized by persistent deficits in social communication and restricted, repetitive patterns of behavior and interest. Sleep problems are not uncommon in children with autism spectrum disorders. Symptoms of insomnia are the most frequent sleep problems in individuals with ASD. Sleep problems can cause significant difficulties in the daily life of children with ASD and their families. Genetic factor, deregulations of melatonin synthesis, extraneous environmental stimuli and psychiatric and medical conditions may cause sleep problems. The first line treatment of sleep problems in ASD includes managements for potential contributing factors and parent education about sleep hygiene care for child and behavioral therapy. Supplementation with melatonin may be effective before considering other medications, such as risperidone, clonidine, and mirtazapine.
Autistic Disorder*
;
Child
;
Autism Spectrum Disorder*
;
Clonidine
;
Education
;
Genetics
;
Humans
;
Hygiene
;
Melatonin
;
Parents
;
Risperidone
;
Sleep Initiation and Maintenance Disorders
7.A Case of Pityriasis Rubra Pilaris Associated with Incidental Acantholysis.
Yang Hoon CHO ; Mu Hyoung LEE ; Choong Rim HAW
Annals of Dermatology 1995;7(4):354-357
Pityriasis Rubra Pilaris is a rare, chronic, mildly inflammatory disease characterized by fine acuminate follicular papules with orange-red to salmon-colored scaling of the skin and erythroderma that surround islands of normal, uninvolved skin, particular in the trunk. Most patients also develop palmoplantar hyperkeratosis in the early course of the disease. Focal acantholysis occurring in pityriasis rubra pilaris is an unusual and incidental histologic finding within the spectrum of histologic change of the disease. No case featuring this concomitant histologic finding has been reported in the Korean literatures up to date. We report a case of focal acantholysis occurring in a patient with the clinical features of pityriasis rubra pilaris which may be considered as an incidental finding.
Acantholysis*
;
Dermatitis, Exfoliative
;
Humans
;
Incidental Findings
;
Islands
;
Pityriasis Rubra Pilaris*
;
Pityriasis*
;
Skin
8.Reconstruction of extensive scalp defect using free latissimus dorsi muscle flap.
Byung Hoon RYU ; Young Seob LEE ; Yang Woo KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1991;18(1):95-100
No abstract available.
Scalp*
;
Superficial Back Muscles*
9.Detection of IgG and IgM Antibodies to Purified Keratinolytic Proteinase in Sera from Patients with Dermatophytosis by Enzyme-Linked Immunosorbent Assay.
Kwang Hoon LEE ; Yang An KIM ; Min Geol LEE ; Jung Bock LEE
Annals of Dermatology 1989;1(1):1-5
No abstract available.
Antibodies*
;
Enzyme-Linked Immunosorbent Assay*
;
Humans
;
Immunoglobulin G*
;
Immunoglobulin M*
;
Tinea*
10.Complete Androgen Insensivity Syndrome in Sisters.
Do Hoon YANG ; Jung Min SIM ; Tae Hoon LEE ; Dong Ik KIM ; Sung Joo HONG ; Min Sung LEE
Korean Journal of Urology 2000;41(5):677-680