Objective To study the protective effect of leuprorelin particle on ovarian reserve function applied before the laparoscopic cystectomy of ovarian endometriosis.Methods 106 patients with endometriosis treated from January 2015 to December 2015 in our hospital were selected.The subjects were divided into two groups according to the random number table, 53 cases of each group.The control group adopted the laparoscopic surgery;the observation group took the leuprorelin particle before the laparoscopic surgery.The operation condition, ovarian hormone and function were observed. Results The operation time and bleeding amount in observation group was better than control group ( P <0.05 ) .After surgery, the exhaust time between two groups had no significantly different.After surgery, the level of follicle stimulating hormone (FSH) in observation group was lower than control group; the level of estradiol (E2) in observation group was higher than control group (P <0.05); the level of luteinizing hormone (LH) between two groups had no significantly different.After surgery, the ovarian volume and antral follicle count ( AFC) in observation group was better than control group (P<0.05).Conclusion Before the laparoscopic cystectomy of ovarian endometriosis, the leuprorelin particle protects the ovarian function and morphology and has less impact in the level of hormone.

Objective To investigate the efficacy and safety of tranexamic acid in perioperative blood loss in old patients with multi-level lumbar spinal stenosis.Methods From January 2009 to September 2010,a total of 68 consecutive patients with multi-level lumbar spinal stenosis ( ≥65 years old) underwent posterior decompression,internal fixation and bone graft fusion who were randomly divided into group A and group B with 34 patients in each.The patients in group A received tranexamic acid and the patients in group B received an equal volume of normal saline.The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion,the number of the patients needing blood transfusion and hemoglobin,fibrinogen,prothrombin time and so on were examined preoperatively and 24 hours postoperatively.All the patients were observed for the clinical symptoms of deep vein thrombosis.Results The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion and the number of the patients needing blood transfusion in group A were significantly decreased than those in group B[ (641.1 ± 128.4) ml vs.(780.1 ± 107.3) ml,(228.80 ± 52.07) ml vs.(345.50 ±42.16) ml,(1.02 + 1.56) U vs.(2.89 ± 1.76) U,16 cases vs.28 cases ],there were significant differences between two groups (P ＜ 0.05 ).As for the value of postoperative hemoglobin concentration in group A [ ( 104.00 ± 4.87) g/L ] was significantly higher than that in group B [ (92.20 + 5.47 ) g/L ] (P ＜ 0.05 ).There were no significant differences in the levels of fibrinogen,prothrombin time,and activated partial thromboplastin time between two groups (P ＞ 0.05).No deep vein thrombosis was found 7 days postoperatively.Conclusion Tranexamic acid can be effectively used in spine surgery in old patients with multi-level lumbar spinal stenosis without increasing the risk of venous thrombosis.

AIM:To study the effect of levofloxacin(LVFX)on serum concentrations and pharmacokinetics of phenytoin sodium(PHT)in rabbits.METHODS:Using UV-spectrophotometry,the serum concentrations of PHT were determined in nine rabbits before oral feeding LVFS and after a single dose of PHT given iv 10 days later.The pharmacokinetic parameters were worked out with 3p87 program.RESULTS:The concentration-time curve of PHT was fit to open two-compartment model.After co-administration of LVFX,the serum concentrations of PHT were decreased to some extent and part of pharmacokinetic parameters obviously changed as well.CONCLUSION:Co-administration of PHT and LVFX will accelerate the metabolic process of PHT.

Objective To determine the role of C-reactive protein(CRP) in evaluating the activity of ulcerative colitis. Methods Analysis was used to estimate the correlation of CRP with mean platelet volume in 41 patients with active ulcerative colitis and 17 patients ulcerative colitis in re-mission. The effects on disease severity were analyzed subsequently. Results Active UC patients sera CRP level(8.58 ±2.43)mg/L,which level of severe UC CRP (14.38 ±4.46)mg/L,the moderate level of CRP UC(6.68 ±2.35)mg/L,mild level of CRP UC(4.94 ±1.21)mg/L, while the remission stage UC patients CRP levels(3.05 ±0. 88)mg/L. CRP was found to be significantly higher in sera of patients with active ulcerative colitis than that in patients in remission (P ＜ 0.05). The sera level of CRP in inflammatory bowel disease at severe stage were significantly higher than those of medium stage (P ＜ 0. 05). CRP was observed to be slightly increased in sera of patients at mild stage (P ＜ 0. 05). Conclusion The increase of CRP could reflect the severity of active ulcerative colitis.

Objective To investigate the effect of serum uric acid (UA) on cardiovascular diseases in hemodialysis patients. Methods The serum level of uric acid and other laboratory indexes of 80 hemodialysis patients were detected,and the left ventricular mass index (LVMI) and left ventricular ejection fraction (LVEF) were detected by ultrasonic cardiography examination. Results According to the serum level of UA, the hemodialysis patients were divided into 2 groups:the group with high serum level of UA and the group with normal serum level of UA. There were significant differences (P<0.05) in hypertension history,the period of hypertension history, systolic blood pressure before hemodialysis between the two groups. The serum levels of UA,creatinine, triglyceride, low density lipoprotein cholesterol, C-reactive protein, brain natriuretic peptide and LVMI were significantly higher,while the serum levels of albumin,pre-albumin,high density lipoprotein cholesterol, LVEF were lower in the group with high serum level of UA than those in the group with normal high serum level of UA (P<0.05) . Furthermore, the serum levels of UA in 80 patients was positively correlated to systolic blood pressure before hemodialysis, serum creatinine, triglyceride, low density lipoprotein cholesterol, C-reactive protein, brain natriuretic peptide, and LVMI (P<0.05), while it was negatively correlated to serum albumin, pre-albumin, high density lipoprotein cholesterol and LVEF (P<0.05) . Conclusion High serum level of uric acid is associated with the onset of cardiovascular diseases in hemodialysis patients.

Gliomas harbor a small population of cells termed glioma stem-like cells (GSC),which have the ability to undergo self-renewal and initiate tumorigenesis.GSCs are resistant to a wide variety of chemotherapeutic agents and possess a remarkable ability to recover from cytotoxic therapy.Furthermore,GSCs play a crucial role in RT failure,as tumors surviving RT are enriched in GSCs.Therefore,an alternative strategy involving selective targeting of this functionally distinct chemo-and radiation resistant small group of GSCs rather than the bulk of the tumor may be more successful in treating this deadly disease.GSC exhibit various alterations to signaling pathway activity,which are associated with self-renewal and neoplastic proliferation.These altered pathways may represent possible targets for GSC.

BACKGROUND: Umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) have been shown to lead to new tissue formation after homing and engrafting to the heart. But the safety of UCB-MSCs engrafting remains to be further investigated. OBJECTIVE: To study the safety and apoptosis inhibition of the UCB-MSCs under co-culture conditions on human cardiomyocytes. METHODS: UCB was collected at delivery with informed consent obtained from 10 donors. The UCB-MSCs were treated with 5-azaserine to induce differentiation into cardiomyocytes. The in vitro cultured cells of the 3rd-5~(th) passages and dividing cells were taken to detect telomerase activity, tumor-related gene expression, G-banding patterns of chromosomal karyotupes, cell surface antigen expression, tumor formation in nude mice, and inhibited apoptosis under co-culture conditions. RESULTS AND CONCLUSION: Prior to and after 5-azaserine induction, telomerase activity and tumor-related gene expression (p53, cyclin A, cdk2, β-actin, C-fos, h-TERT, c-myc) of UCB-MSCs were similar, no abnormal chromosomal karyotupes were observed, immunophenotype exhibited no change, CD34 was negative, but CD44 and CD90 (Thy-1) were positive. At 10 weeks after inoculation of UCB-MSCs, nude mice still survived healthily and no formed tumor in vivo was observed. Hematoxylin-eosin staining suggested normal subcutaneous tissue. Compared with simple cardiomyocytes, UCB-MSCs could significantly inhibit cardiomyocyte apoptosis under co-culture conditions (P < 0.05), indicating that human UCB-MSCs are a valuable, safe, and effective source of cell transplantation treatment.

OBJECTIVE:To explore the treatment effect and complication prevention of various stent materials for intracranial arteriostenosis.METHODS:A computer-based online search of Science Direct and Ei database (1998-01/2009-10) was performed for English articles with the key words of "intracranial arteriostenosis,stent placement" In addition,CNKI and CBM database (1998-01/2009-10) were searched for related Chinese articles with the same key words in Chinese.Moreover,related works were manually searched.Studies regarding various stent materials for intracranial arteriostenosis were included,including basic and clinical experiments.RESULTS:Multicenter studies of worldwide show that stent implantation displays obvious advantages in treating carotid artery stenosis compared with carotid endarterectomy.Recently,with developing of balloon and stent compliance,as well as stent technology,and application of various novel materials and cerebral protection device,stent has become a safe and effective therapy for intracranial arteriostenosis.However,complications following stent implantation,such as restenosis,ischemic stroke,cerebral hemorrhage hypertransfusion syndrome,and cerebral angiospasm,limit its application.CONCLUSION:The safety and efficacy of intracranial stent implantation remains validation of multicenter and perspective studies.High incidence of restenosis following stent implantation requires further improvement.

Abdominal wall endometriosis (AWE) is defined as endometrial tissue superficial to the peritoneum. Many cases of AWE are associated with cesarean section scars. Thus, AWE is often misdiagnosed as a hernia or hematoma. The most common presentation of AWE is the development of a painful mass after uterine surgery. Many patients with AWE are associated with a previous surgical incision. Careful history and physical examination is essential to make correct diagnosis. Studies such as ultrasound, CT, MRI, and FNA are helpful. Medical treatment of AWEs is usually unsuccessful. Therefore, surgical treatment should be offered to every patient and the results usually are excellent. We performed a review of published literatures to demonstrate the pathogenesis, symptoms, diagnosis, treatment and outcomes of patients having AWE.

Heart Failure ; therapy ; Humans ; Practice Guidelines as Topic