Objective To study the protective effect of leuprorelin particle on ovarian reserve function applied before the laparoscopic cystectomy of ovarian endometriosis.Methods 106 patients with endometriosis treated from January 2015 to December 2015 in our hospital were selected.The subjects were divided into two groups according to the random number table, 53 cases of each group.The control group adopted the laparoscopic surgery;the observation group took the leuprorelin particle before the laparoscopic surgery.The operation condition, ovarian hormone and function were observed. Results The operation time and bleeding amount in observation group was better than control group ( P <0.05 ) .After surgery, the exhaust time between two groups had no significantly different.After surgery, the level of follicle stimulating hormone (FSH) in observation group was lower than control group; the level of estradiol (E2) in observation group was higher than control group (P <0.05); the level of luteinizing hormone (LH) between two groups had no significantly different.After surgery, the ovarian volume and antral follicle count ( AFC) in observation group was better than control group (P<0.05).Conclusion Before the laparoscopic cystectomy of ovarian endometriosis, the leuprorelin particle protects the ovarian function and morphology and has less impact in the level of hormone.

Endothelial Cells ; metabolism ; physiology ; Stem Cells ; metabolism ; physiology

Objective To investigate the role of chemokine in inducing peripheral arthritis in spondyloarthropathy(SpA).Methods Microarray was used to screen the candidates of inducing peripheral arthritis in SpA,and enzyme linked immunosorbent assay(ELISA)was used to verify the results.Peripheral blood mononuclear cells(PBMC)from 5 healthy subjects were co-incubated 1.5 hours with synovial fluid of 5 patients with SpA,total RNA were then extracted,and the samples were screened for the target genes by using superarray techniques.ELISA was used to determine CXCL1 protein levels of 54 ankylosing spondylitis(AS)patients and 30 healthy controls,also to determine the CXCL1 levels of synovial fluids from 33 SpA patients and 14 osteoarthritis(OA)patients,and to determine CXCL1 protein levels by ELISA before and after treatment with infliximab.Results The expression of CXCL1,CXCL2,CXCL3 and IL-8 were more than 2 fold higher after stimulated by synovial fluids.However,there was no change in other chemokines and cytokines,such as TNF?,IL-6 and IL-1 after stimulation.The protein level of CXCL1 was significant higher in serum of patients with AS than that of healthy control(P

Objective To clone, locate and differentially analyze the transcription factor Relish gene from Anopheles stephensi, and to examine its signals-modulating action on prophenoloxidase cascade and melanization of Plasmodium yoelii oocysts. Methods Relish cDNA of total mosquitoes was amplified by RT-PCR with degenerated primers. Target PCR product was purified,cloned,sequenced and identified. Special Relish gene was amplified with specific primers from hemocytes or midgut,respectively. Semi-quantitative analysis was made under different feeding conditions. Relish message ribonucleic acid was identified with hybridization in situ. Results One cDNA segment of Relish similar to An.gambiae was acquired from An. stephensi. The same Relish gene was also manifested in the hemocytes and midgut. Marked up-regulation expression of Relish was observed at 6,12,24 or 48 h of Plasmodium yoelii infection and at 12 and 24 h after sucking nitroquine-acetate sucrose solution,that was before inducible oocyst melanization. Relish was also expressed in the hemocytes and midguts by ISH. Conclusion Transcription factor Relish of An. stephensi might play a role in signal modulation of Plasmodium yoelii infection and oocyst melanization.

Objective To investigate the significance of myelography for selecting operation methods in microendoscopic discectomy(MED).Methods A total of 196 patients with lumbar disc herniation diagnosed by CT/MRI received a myelography before MED.According to their clinical manifestations,signs,and outcomes of myelography,the patients were given three different operation methods.①Simple discectomy was performed in 105 patients with patent spinal canal,lateral impression at intervertebral level,and shift of the nerve root sleeve;②Discectomy with spinal decompression was performed in 70 patients with patent spinal canal and compression of the nerve root sleeve;③Discectomy with semilaminectomy was performed in 21 patients with patent spinal canal,semispherical compression of the nerve root sleeve,and 7-shaped nerve root.Results The operation time was 30~90 min(mean,50 min) and the intraoperative blood loss was 30~120 ml(mean,50 ml).No intraoperative complications occurred.Conversions to open surgery were required in 21 patients,including partial spinal stenosis in 16 patients and thickened vertebral plate in 5 patients.Follow-ups were carried out in 196 patients for 3~58 months(mean,34 months).According to the Japanese Orthopedic Association(JOA) scoring system,the final scores significantly increased postoperatively(25.86?2.96) than preoperatively(13.21?4.96)((t=)38.24,P=0.00).According to the Macnab and Watts standard,the rate of excellent or good results was 93.7%(164/175).Conclusions Myelography has an important reference value for selecting operation methods of microendoscopic discectomy in patients with lumbar disc herniation.

In order to solve the problems about the lack of investing capital and to evade investing risk,a military hospital can achieve better economic benefit by introducing cooperative medical items.This method can not only make full use of its own good invisible assets and medical resource,but also help to raise its level of medical service and expand its market of medical service.To make a good item of cooperative medical service,special attentions must be payed to the following aspects such as prophase demonstrating,contract signing,partitionning of income and expenditure,financial accouting and daily accouting.

Objective To analyze the clinical efficacy of gemcitabine plus oxaliplatin in treatment of relapsed or refractory non-Hodgkin's lymphoma(NHL).Methods 39 cases with relapsed or refractory NHL were treated by gemcitabine and oxaliplatin combination chemotherapy. After three cycles, its efficacy and adverse reactions　were observed. Serum lactate dehydrogenase(LDH) concentration was detected before and after treatment and all the　patients were followed up. Results The rate CR was 11cases (28. 1％ ), PR 16cases (41.03％), NC 7cases (17.95％),PD 5cases(12.82％).Thetotalresponserate(CR+PR) was 69. 23％. Conclusion Gemcitabineand oxaliplatin combination chemotherapy in treatment of patients with relapsed or refractory NHL was safe and effective.

ObjectiveTo study the heat shock protein 47 ( HSP 47 ) andtype Ⅰ ,Ⅲ collagen relevance expression in keloid tissue. MethodsUSE immunohistochemistry to detect 21 patients( HS group) pathological keloid tissue HSP47, Ⅰ-type, Ⅲ collagen levels,and normal foreskin(control group) compared to the expression of HSP 47 and Ⅰ and Ⅲ collagen. ResultsHS group HSP 47 scar absorbance was( A value) ( 133.0 ± 58.7) ,it was significantly lower than the control group( 169.6 ± 53.2) ( t =2. 697 ,P =0.024) ;two groups of collagen type Ⅰ, Ⅲ collagen levels had statistically differentce(t =14.898,12.610,all P =0.000) ; HSP 47 level and type Ⅰ collagen were positively correlated(r =3.058,P =0. 034),and type Ⅲ collagen content was also positively correlated(r =4. 610, P =0. 029). ConclusionHSP 47 in keloid tissue could cause dermal layer assembly of collagen synthesis and significantly increased,and it was an important mechanism for the formation of keloids.

Objective To investigate the efficacy and safety of tranexamic acid in perioperative blood loss in old patients with multi-level lumbar spinal stenosis.Methods From January 2009 to September 2010,a total of 68 consecutive patients with multi-level lumbar spinal stenosis ( ≥65 years old) underwent posterior decompression,internal fixation and bone graft fusion who were randomly divided into group A and group B with 34 patients in each.The patients in group A received tranexamic acid and the patients in group B received an equal volume of normal saline.The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion,the number of the patients needing blood transfusion and hemoglobin,fibrinogen,prothrombin time and so on were examined preoperatively and 24 hours postoperatively.All the patients were observed for the clinical symptoms of deep vein thrombosis.Results The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion and the number of the patients needing blood transfusion in group A were significantly decreased than those in group B[ (641.1 ± 128.4) ml vs.(780.1 ± 107.3) ml,(228.80 ± 52.07) ml vs.(345.50 ±42.16) ml,(1.02 + 1.56) U vs.(2.89 ± 1.76) U,16 cases vs.28 cases ],there were significant differences between two groups (P ＜ 0.05 ).As for the value of postoperative hemoglobin concentration in group A [ ( 104.00 ± 4.87) g/L ] was significantly higher than that in group B [ (92.20 + 5.47 ) g/L ] (P ＜ 0.05 ).There were no significant differences in the levels of fibrinogen,prothrombin time,and activated partial thromboplastin time between two groups (P ＞ 0.05).No deep vein thrombosis was found 7 days postoperatively.Conclusion Tranexamic acid can be effectively used in spine surgery in old patients with multi-level lumbar spinal stenosis without increasing the risk of venous thrombosis.

OBJECTIVE:To analyze the related factors that initiating the occurrence of adverse drug reactions (ADR). METHODS: The 184 ADR reports occurred from April, 1999 to July, 2004 were analyzed by a descriptive research method. RESULTS: Among the 184 cases, female cases outnumbered the male cases with the ratio at M∶F=1∶1.4, 122 cases were the elderly at the age of more than 60 which accounts for 66.30% of the total;there were 123 single medication cases and 61 combined medication cases;there were 23 severe cases, 128 moderate cases and 33 mild cases; 153 patients were symptomatically treated, 31 cased were not given special treatment;161 patients were fully recovered, 17 cases showed some improvement and 6 cases did have sequela;in the cause-effect relationship assessment, there were 3 certain cases, 153 probable cases and 28 possible cases; the anti-infective-led ADR accounted for 64.39%, then came the central nervous system drugs and Chinese traditional medicines at a percentage of 12.33% and 10.55% respectively;skin and its appendages impairment cases were the most with a percentage at 67.55%.CONCLUSION: To lessen or avoid the occurrence of ADR, monitoring of which and publicizing of ADR knowledge should be emphasized.