Sepsis,a common clinical syndrome,comparing to septicaemia or bacteremia,has a more comprehensive pathophysiological process.Its pathogenesis is complex,including pro-inflammatory/anti-inflammatory cytokine imbalance,intestinal bacterial translocation,immunosuppression,signal pathway activation,etc.Further study on the pathogenesis of sepsis is the theory foundation to explore the new method of sepsis.The diagnosis of sepsis pathogenesis,treatment principles and common virus infection induced sepsis are reviewed in this paper.

Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; therapy ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Hepatitis B ; complications ; drug therapy ; Humans ; Liver Cirrhosis ; drug therapy ; etiology ; Medicine, Chinese Traditional ; Phytotherapy

Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; Humans ; Phytotherapy ; methods ; trends

Objective To explore the protective effect mechanism of minocyline on the model of the cerebral ischemia-reperfusion of gerbils by decreasing expression of phosphorylation P38MAPK.Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids.The ultrastructural alteration in pyramidal neuron in the hippocampal CA1 and the cortex of frontal lobe at different time was observed by electron microscope.And observe the expression level of phosphorylation P38MAPK by using immunohistochemistry technique.Results ①The damage to the neunos became more and more severe as gerbils survived longer postocclusion.Minocyline treated groups were lessened obviously.② Treatment with minocycline reduced expression of phosphorylation P38MAPK with a significant difference(P

During 1920-30s,under the push of Kuomintang,Communist Party and social personage,the Chinese village launched a cooperation movement. Although they failed to renew rural areas in China,the farmer autonomy spirit, policy practicality,the educational principles,and the spirit of evidence-based demonstration research embodied in the movement still have an enlightening significance to the construction of the new rural cooperative healthcare system and construction of new-type villages.

Vasculogenic mimicry (VM) is a term that describes a novel form of angiogenesis, channels of which are patterned networks of interconnected loops of periodic acid-Schiff (PAS)-positive extracellular matrix forming by highly aggressive tumor cells instead of endothelial cells. VM is closely correlated with the invasiveness, metastasis and prognosis of related tumors. However, traditional anti-vascular therapies aiming at endothelial cells have no remarkable effects on malignant tumor with VM. Therefore, VM should be an important factor to consider in design of anti-angiogenesis therapies of highly aggressive tumors and it is expected to become a potential target for tumor therapy. Some recent progresses of anti- vasculogenic mimicry therapy are reviewed in this article.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

ObjectiveTo investigate the effects of X-linked inhibitor of apoptosis (XIAP) siRNA or XIAP antagonist Embelin on the growth inhibition and apoptosis of hepatcellular cancer cell line HepG2,which was treated by tumor necrosis factor-related apoptosis (TRAIL). Methods HepG2 cells were tranfected either by XIAP siRNA or a negative control,followed by treatment with TRAIL,Embelin or a combination of the two.XIAP expression,cell growth,and caspase-3 activity were determined by Western blot,MTT assay and fluorescent caspase-3 assay,respectively.Cleaved PARP expression levels of were measured by Western blot.ResultsThe XIAP protein expression was significantly downregulated by transfection with XIAP siRNA.Compared with the negative control,the XIAP siRNA significantly inhibited the growth of HepG2 cells treated by 100ng/ml(6.8％ ±1.2％ vs.11.8％±4.0％,P＜0.05)and 1000 ng/ml (18.9％ ±2.0％ vs.26.6％ ±1.5％,P＜0.01)by TRAIL.TRAIL-induced caspase-3 activation and PARP cleavage were also increased significantly by XIAP siRNA transfection.In addition,Embelin also significantly inhibited cell growth (P＜0.01),activation of caspase-3 (P＜ 0.01) and TRAIL-induced PARP cleavage.Conclusions Both XIAP siRNA and Embelin may potentially contribute to clinical treatment of hepatocellular carcinoma.

Objective To analyze the concentration changes of serum hypoxia stress peptide (HSP)and plasma nitrogen monoxidum(NO)in the entering mountain rapidly man and to explore the importance of HSP and NO in adaptation of man to altitude hypoxia.Methods The levels of serum HSP and plasma NO2-/NO3-were measured from 106 entering mountain rapidly men,105 altitude in habitant,105 altitude immigrant and 104 plain healthy controls,respectively by chromatometry and NO2-/NO3-reagent.Furthermore,the content of serum HSP was determined in 106 entering mountain rapidly men,respectively at the time of 24 h,48 h,76 h,l week and 1 month after entering mountain.Results The level of serum HSP was significantly higher in entering mountain rapidly men than in habitant,immigrant and plain controls(P＜0.05),while there was no statistical difference between in habitant,immigrant and plain controls(P＞0.05).At 24h after entering mountain rapidly,the level of serum HSP was significantly increased and reached the peak.At 48h and 76h,it was slightly decreased,but still maintained a high level.At 1 week and 1 month,it gradually and significantly went down,but was still significantly higher than that in plain controls.Similarly,the level of plasma NO2-/NO3-was significantly elevated in entering mountain rapidly man,compared with inhabitant,immigrant and plain controls(P＜0.05).It was lower in inhabitants and immigrants than plain controls,but the difference was not statistically significant(P＞0.05).Conclusion The present results demonstrated that the levels of serum HSP and plasma NO were significantly up-regulated in healthy adults after en tcring mountain rapidly in order to adapt to altitude stress,and restored gradually to the normal level as time went by.