Reverse transcription loop-mediated isothermal amplification(RT-LAMP)is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection.Compared with reverse transcriptase polymerase chain reaction (RT-PCR),the RT-LAMP has advantages of reaction simplicity,rapidly,cost-effective,detection sensitivity and specificity.Nowadays,RT-LAMP technology is gradually used in various medical fields,but it is still a new method especially in cancer diagnosis of tumor gene.The article reviews the application of the RT-LAMP technology in the cancer

Researches indicate that gastric carcinoma occurs where gastric stem cells settle down.Stem cells may play an important role in formative process of gastric carcinoma. Researches on relationship between stem cells and gastric stem cells, tumor stem cells and stomach neoplasms, bone marrow stem cells and stomach neoplasms indicate that gastric carcinoma may be a kind of stem cell diseases.

Useful pharmacodynamic changes occur when some Chinese medicine are used together with some Western medicine, namely enhanced curative effect, lowered adverse reactions, reduced dosages, shortened treatment courses, enlarged indications scope, improved compliance of treatment and rational medication, which could be explored to provide scientific bases for further improving diagnosis and treatment levels and rational use of drugs.
Drugs, Chinese Herbal ; metabolism ; Humans ; Medicine, East Asian Traditional ; Pharmaceutical Preparations ; metabolism ; Pharmacokinetics ; Phytotherapy

Chemotherapy resistance is a great obstacle for effective treatment of ovarian cancer.Drug resistance of ovarian cancer is the results of combined action of multi- factors.Further study of drug resistancerelated genes can provide new ideas for reversing drug resistance fundamentally.

Carcinogenesis ; pathology ; Colitis ; pathology ; Humans

Bone Neoplasms ; immunology ; pathology ; Child ; Humans ; Osteosarcoma ; immunology ; pathology ; Rosette Formation

Objective To explore the feasibility and application value of low dose CT with automatic tube current modulation (ATCM) in the diagnosis of pneumoconiosis.Methods Forty-five patients with pneumoconiosis confirmed by the occupational agency underwent standard dose CT scanning with a fixed tube current of 150 mA and low dose CT scanning with ATCM (50-200 mA) with noise index (NI) of 14.The CT dose index volume (CTDIvol) and dose length product (DLP) under the different scanning condition were recorded.The image quality,specific image manifestations of pneumoconiosis (shadow size,shadow concentration,pneumoconiosis stages) were reviewed in double-blind methods by two radiologists,and the statistical analysis was performed.Results CT characteristics of pneumoconiosis could be detected efficiently with low dose CT scanning with ATCM.There were no significant differences in showing the shadow size,shadow concentration,pneumoconiosis stages and the image quality between the two groups (P＞0.05).The CTDIvol and DLP of standard dose CT scanning were 13.53 mGy and (337.13 ± 13.53) mGy/cm respectively,and CTDIvol and DLP of low dose CT scanning with ATCM were (7.39 ± 0.45) mGy and (188.78 ± 1.80) mGy/cm,there were statistical differences (P ＜ 0.05).Conclusions Low dose CT scanning with ATCM can reflect the characteristics of pneumoconiosis,and it also can significantly reduce the radiation dose with diagnostic acceptability of the image quality.So low dose CT scanning with ATCM can replace the standard dose CT scan in diagnosing and screening of the pneumoconiosis.

10.3969/j.issn.2095-4344.2013.25.026

Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods：The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1，3，5，7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results： There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.

ObjectiveTo explore the roles of Toll-like receptor (TLR) 3,7 and 9 in the pathogenesis of pityriasis rosea.MethodsThis study recruited 40 patients with pityriasis rosea and 40 normal human controls.Reverse transcription-PCR was carried out to detect the expressions of TLR3,7 and 9 in peripheral blood lymphocytes of these subjects.ResultsThe mRNA expressions of TLR3,7 and 9 in peripheral blood lymphocytes were significantly higher in patients with pityriasis rosea than in the normal human controls (Z =4.239,3.745,2.658,all P ＜ 0.05).There was a positive correlation between the expression of TLR3 and TLR7(r =0.574,P ＜ 0.01 ) as well as between that of TLR3 and TLR9 (r =0.551,P ＜ 0.01 ) in the patients,while there was no correlation between TLR7 and TLR9 expression in the patients or between the expressions of TLR3,TLR7 and TLR9 in the controls.ConclusionVirus infection might be involved in the pathogenesis of pityriasis rosea via TLR pathways.