Selected mainly were acupoints in the adjacent areas and along the meridians, assisted by ashi points. Fengmen (BL 12), Geshu (BL 17) and Xuehai (SP 10) were selected for migratory arthralgia;Shenshu (BL 23) and Guanyuan (CV 4) for cold-aggravated arthralgia; Pishu (BL 20), Zusanli (ST 36)and Yinlingquan (SP 9) for damp arthralgia; Dazhui (GV 14) and Quchi (LI 11) for heat-induced arthralgia. Acupuncture in combination with far infra-red radiation was performed.76 patients with rheumatoid arthritis were treated and 74 cases with herb therapy as control. The result showed that there was statistically significant difference in curative effect between the 2 groups, χ 2=10.18, P＜ 0.01, indicating that the curative effect was significantly better in the treatment group than in the control group. Statistics showed that there was no significant difference in curative effect among the 4 syndromic types, namely migratory arthralgia, cold-aggravated arthralgia, damp-induced arthralgia and heat-induced arthralgia. But there was a statistically significant difference in curative effect between the above 4 syndromic types and deficiency-type arthralgia, indicating that the curative effect in deficiency-type arthralgia was worst.

Objective:To investigate the clinical efficacy ofacupuncture plus Tuina therapy in treating supraspinatus tendinitis.Methods:One hundred patients with supraspinatus tendinitis were randomly allocated to two groups.The treatment group of 50 cases was treated with acupuncture plus Tuina therapy and the control group of 50 cases,with simple acupuncture.Treatment was given once daily,10 times as a course.The therapeutic effects were evaluated after two courses oftreatment.Results:The total efficacy rate was 96.0%in the treatment group and 74.0%in the control group.x2 test showed that there was a statistically significant difference in the total efficacy rate between the two groups(P＜0.01).Conclusion:Acupuncture plus Tuina therapy is superior to simple acupuncture in treating supraspinatus tendinitis.

Objective:To investigate the effect of miR-26b on the invasion and migration of lung cancer cell and to explore its mechanism.Methods:qPCR was used to detect the expression of miR-26b in lung cancer.Luciferase reporter gene was used to detect interaction between miR-26b and hENT1.Transwell assay was used to detect invasion ability after treatment of miR-26b mimics.Scratch assay was used to detect migration ability after treatment of miR-26b mimics.The expressions of hENT1,ROCK-I and RhoA were detected by Western blot.The changes of cytoskeleton after miR-26b mimics treatment with phalloidin were observed.The effect of miR-26b mimics on the tumor size and volume of lung cancer was determined by subcutaneous tumor formation in nude mice.Results:MiR-26b expression was significantly reduced in lung cancer.With the progress of lung cancer,the expression of miR-26b was reduced.With the progress in differentiation of lung cancer,the expression of miR-26b was decreased.Decrease of miR-26b was associated with lung cancer lymph node metastasis.HENT1 was the direct target of miR-26b;miR-26b regulated the invasion and migration ability of human lung carcinoma A549 cells.MiR-26b regulated the expression of hENT1,ROCK-1 and RhoA.After the treatment with miR-26b mimics,the F-actin staining was significantly reduced,whereas the formation of wrinkles and the formation of pseudopodia were significantly reduced.Subcutaneous tumor formation in nude mice showed that miR-26b mimics treatment significantly reduced the tumor size and mass.Conclusion:MiR-26b plays a role in tumor suppression in lung cancer.miR-26b can regulate the invasion and migration ability of lung carcinoma A549 cells by targeting hENT1 depending on the RhoA/ROCK-1 pathway.

MicroRNAs are the endogenous non-coding RNAs that regulate gene expression by mediating gene post-transcriptional silence.Among them,miR-21,miR-17-92,miR-214,miR-26a,miR-221,miR-222and so on mainly suppress the expression of PTEN phosphohydrolase through targeting PTEN mRNA 3'-UTR.PTEN plays an important role in cell proliferation,apoptosis,migration and invasion,and its low expression will induce tumorigenesis.

The minimally invasive approach is the direction of cardiac surgery.With the development of telemanipulation and computer technology,the minimally robotic surgery has become reality.As the one of the most minimally invasive cardiac surgery,the robotic surgical system provides cardiac surgeons with less invasive manner and dexterity manipulation.Robotic cardiac surgery is an evolutionary process and has resulted in a substantial increased attention to surgeons and patients.This review is to introduce the recent clinical application of robotic technology in cardiac surgery.

With the development of molecular biology,molecular targeted therapy has become main direction and trend at the aspect of oneotherapy in the 21th century.Molecularly targeted agents which target VEGF/VEGFR,EGFR.Raf/MAPK-ERIC,HGFR,as well as associating with other therapies are available in the clinical treatment of primary hepatic carcinoma,and inspiring results have also achieved in some clinical trials.The review attempts to summarize the clinical research advancemem in molecularly targeted therapy of primary hepatic carcinoma.

ObjectiveTo investigate the prevention and management of the hemorrhage during laparoscopic cholecystectomy( LC), and improve success ratio. MethodsThe medical data of 420 cases with LC from May 2003 to 2009, September were analyzed retrospectively, including total number, hemorrhage site and ways of prevention and management. Results417 cases experienced successful C,with success ratio 99.3%. 49 cases ( 11.7% ) had hemorrhage, and in 46 cases(93. 9% ), it was stopped by laparoscope or hemostasis by compression,3 cases were rescued by operation. All hemorrhage sites included 15 cases(30. 6% ) in triangle de Calot,26 cases(53. 1% ) in gallbladder bed, and 8 cases ( 16.3% ) in incision. ConclusionA reliable opetration and appropriate hemostasis are critical of the successful operation and prevention of complications after LC.

Fracture Fixation ; instrumentation ; methods ; Fractures, Bone ; surgery ; Humans ; Scaphoid Bone ; injuries ; surgery ; Wrist Injuries ; surgery ; Wrist Joint ; surgery

OBJECTIVE:To investigate the number of insoluble particle in total parenteral nutrition(TPN)solutions and to pro-vide reference for clinical application. METHODS:6 kinds of commonly used TPN prescriptions were selected. After prepared ac-cording to sterile operation procedure,TPN at 25℃was investigated at 0,1,4,8 and 24 h in terms of appearance,pH value,os-motic pressure and the numbers of insoluble particles. RESULTS:The appearance and pH value of TPN had no significant change within 24 h after preparation,and osmotic pressures were all lower than 900 mOsm/L. Study results showed that the numbers of in-soluble particles quickly increased,and ≥10 μm and ≥25 μm insoluble particles exceeded the limitation of Chinese Pharmacopoe-ia after Lipid-soluble vitamin for injection were injected into TPN. Medium/long chain fat emulsion injection,water-soluble vita-min,electrolytes and trace elements also increased the numbers of insoluble particles. CONCLUSIONS:Medium/long chain fat emulsion injection,lipid-soluble/water-soluble vitamin,vitamin C,electrolytes and trace elements have certain influence on the number of insoluble particle of TPN. To analyze the reason for the forming of insoluble particles has great meaning both for improv-ing the TPN quality and reducing the harm of particles for patients.

Bone Marrow Mesenchymal Stem Cells (BMSCs) are ideal seed cells for cell therapy,which have been through many long-term studies of in vitro and animal disease model treatment and have been widely used in preventing and treating a variety of clinical diseases caused by aging and other damage,including bone injury and regeneration,neural degenerative and injury,myocardial injury,liver cirrhosis,diabetes,lung disease and so on.BMSCs application in the treatment of orthopaedic disease is the most common and effective.By retrospective literature review,we summarize the BMSCs transplantation application in Orthopaedic disease treatment.As an ideal cell source,BMSCs was first applied in bone tissue engineering,mainly used in the treatment of long bone defect and bone nonunion;Recently,the research trends of clinical BMSCs application turned to treat degenerative diseases and genetic disorders because of its great potential,including osteoarthrits,femoral head necrosis,disc degeneration,spinal cord injury,knee varus,osteogenesis hypoplasia,hypophosphatasia and so on.The safety and efficacy of BMSCs treatment are the key issues in clinical cell therapy.There is no side effects and complications after BMSCs treatment,prove its clinical application is safe.But due to type and degree of disease and individual differences,therapeutic methods including injection method,effective concentrations,treatment window selection,cycle and therapeatic effects have a bigger difference.There is still no uniform of recognized operation standards for the treatment of the same type of disease.So BMSCs transplantation requires effective control during whole medical process and its application in orthopedic diseases is still lack of large-scale multicenter clinical study.Therefore,this review focuses on induction,summary and analysis in the research status of BMSCs in bone disease treatment,and provide new ideas and methods for BMSCs transplantation in orthopaedic disease prevention and treatment.