Objective: To study the characteristics of mouse embryonic stem (ES) cell line R1 expressing green fluorescent protein (GFP) efficiently and stably. Methods: Four kinds of biological characteristics: growth curve, express level of alkaline phosphatase, karyotype and pluripotentiality of the subclones of R1 ES cell expressing GFP were observed and analysed. Results: No obvious differences between R1 ES cells and the 4 ES cell clones expressing GFP［ESG（+）］ on the proliferation speed, differentiation state and the ratio of normal karyotype were observed. Teratocarcinoma concluded 3 germinal layers could form after inoculating ESG(+) cells to nude mice. Conclusion: The expression of GFP may not make any detectable effect on the proliferation speed, differentiation state and the pluripotentiality of R1 ES cells. This research work ensures efficient utilization of GFP as a reporter gene tracing ES cell in vivo.

Oxaliplatin is effective for the treatment of advanced colorectal cancer,but its application is restricted because of the dose-limiting toxicity.As drug carriers,liposomes are featured with delay release,lesion targeting and they could decrease the side effects of drugs.Active targeting modification of the liposome can change the biological distribution of the anticancer drugs,reduce or reverse multidrug resistance of tumor cells and enhance the effect of anti-tumor drugs.With these characteristics mentioned above,lipsomes containing oxaliplatin may play an important role in the treatment of colorectal cancer.

neoplasia.

Serum soluble IL- 2 receptor (sIL-2R) level of 34 patients with leukemia were measured by ELISA. The sIL- 2 R levels are 296?99u /ml in normal, 660 ?449u /ml in AML, 1323 ?755 u/m in ALL, 1577 ? 759 u /ml in AMML and 1815 ? 858 u /ml in NHL. 5 of 6 patients with 2000u /ml sIL- 2 R died in one month after detection of sIL-2R. There is no apparent relationship between sII -2 R level and mononuclear cells count in peripheral blood. The results suggest that it is possible to be a new parameter for the observation and prognosis estimation to patients with leukemia by detection of sIL-2R.

There is still a certain recurrence rate after extensive lymphadenectomy even to patients with node-negative gastric cancer.It promotes the researchers to use a more sensitive and effective way to track tumor cells which are missed,especially lymph node micrometastases.With the development of detection tech-nology,the diagnostic rate of micrometastasis is significantly increased.There are so many controversies about the impact of lymph node micrometastases that no consensus on the clinical treatment can be reached.In recent years,with the rise of endoscopic therapy,how to balance the relationship between the quality of life and the safety makes the research of micrometastases more urgent.

Objectives To explore the effects of Baicalin and Berberine against Trichomonas vaginalis(TV) in vitro. Methods The micro-double-dillution assay is used to measure the Minimal lethal concentration (MLC) of Baicalin and Berberine against 6 freshly isolated strains of TV, using metronidazole as control. Results MLC of Baicalin and Berberine against 6 isolated strains in vitro of TV ranges from 0.125 to 0.5 mg/mL and 0.25 to 1.0 mg/mL, respectively. The means of MLC are 0.229 mg/mL and 0.417mg/mL, respetively, with statistically significant difference (t=2.666, P

Communicable Disease Control ; methods ; trends

Aged ; Aged, 80 and over ; Contraindications ; Humans ; Pacemaker, Artificial

With the development of modern science,the updates of endoscopic technology,respiratory endoscopy has become essential means in the clinical diagnosis and treatment of respiratory disease in children.Due to the particularity of its complex structure and material,to strengthen the cleaning,disinfection and sterilization of respiratory endoscopy and attachment can ensure medical safety,prolong the service life of the endoscopic.It also was the primary guarantee to ensure that the treatment of respiratory endoscopy proceed smoothly and securely.