Objective To investigate the prognostic factors of young stroke patients with conventional treatment,and provide the basis for clinical diagnosis and treatment of stroke.Methods The clinical data of 72 cases of young stroke patients with conventional treatment were analyzed retrospectively.The prognostic factors were analyzed.Results In seventy-two cases of young stroke patients,18 cases of conventional treatment failed (25.00％,18/72).Univariate analysis showed that smoking,alcohol,underlying disease,dysphagia,barthel index (BI) score,U.S.national institutes of health stroke scale (NIHSS) score and Oxford handicap scale (OHS) score was closely related with the prognosis (P ＜ 0.05 or ＜ 0.01).Logistic analysis showed that the age,BI score,NIHSS score,OHS score and underlying diseases was the independent prognostic factor for young stroke patients.Eighteen cases who failed in conventional treatment fails accepted comprehensive rehabilitation treatment.Compared with that before treatment,1,3,6 months after treatment BI,NIHSS and OHS scores were significantly decreased (P ＜ 0.05).Conclusions BI,NIHSS,OHS score and underlying diseases are the independent prognostic factor for young stroke patients.Surgery and postoperative comprehensive rehabilitation in young stroke patients who failed in conventional treatment can improve patient's outcomes and prognosis.

Dexamethasone ; therapeutic use ; Glucocorticoids ; pharmacology ; therapeutic use ; Humans ; Infant, Newborn ; Meconium Aspiration Syndrome ; drug therapy ; physiopathology ; Methylprednisolone ; therapeutic use ; Prednisolone ; therapeutic use

OBJECTIVETo research the mechanism of dopamine (DA) controlled memory in mice.

METHODSMice received i.p. injection of scopolamine (0.3 mg/kg, SCOP 0.3, and 3.0mg/kg, SCOP 3.0, respectively, n = 10) and saline (NS, n = 10) for 60 days in experiment 1. Memory of mice was detected by dark avoidance behavior in the 53" d and the 60"' d. Animals were sacrificed after the memory test; brain tissues were processed for Fos-ir and TH-ir by immunohistochemistry. Mice were divided into four groups according results of expri-ment 1, they received i.p. injection of apomorphine (0.1 mg/kg, APO 0.1, 0.5 mg/kg, APO 0.5, and 2.0 mg/kg, APO2.0 respectively, n = 10).

RESULTSMemory was inhibited in mice injected scopolamine 3.0 mg/kg. Latency was significantly less than in NS group, only 1/ 4 that of NS group (P > 0.05). The number of mistake of SCOP 3.0 group increased about four times than that of NS group (P > 0.05). But there was no difference of latency and number of mistake between SCOP 0.3 and NS group in expriment 1. Scopolamine-induced memory deficit was associated with decreased cellular activation, indicated by Fos immunoreactive (ir) staining, in NAcc CA1 and CA3 (P < 0.05), and also associated with decreases in the number of cells labeled for tyrosine hydroxylase (TH-ir), the rate limiting enzyme for dopamine conversion (P < 0.01) and the number of cells co-labeled for TH-ir/Fos-ir (P <0.01) in the ventral tegmental area(VTA), apomorphine lessened scopolamine-induced memory deficit in experiment 2. The number of cells co-labeled for TH-ir/Fos-ir (P <, 0.05) was increased in VTA after apomorphine treatment.

CONCLUSIONApomorphine lessened scopolamine-induced memory deficit in mice by increasing DA activities in VTA.

Animals ; Apomorphine ; pharmacology ; Disease Models, Animal ; Dopamine Agonists ; pharmacology ; Male ; Memory Disorders ; chemically induced ; drug therapy ; Mice ; Scopolamine Hydrobromide ; toxicity

Objective To explore the efficacy of GM6001,tissue inhibitor expression and significance of matrix metalloproteinase?9(MMP?9)in mice model of oxygen?induced retinal neovascularization(RNV)and evaluate the inhibition effect of MMP?9 inhibitor(GM6001)on RNV. Meth?ods Mice were placed in oxygen boxes to establish oxygen?induced RNV animal models. The GM6001 treated or hyperxia control groups received an intravitreal injection of 1μL GM6001(100μmol/L)or PBS at day 11 after birth. The normal control and hyperxia group were not treated. HE staining was used to detect RNV in retinal whole mounts,the mRNA level and protein expression of MMP?9 were measured by RT?PCR,Western blot and immunohistochemistry,respectively. Results RNV in the GM6001 treated group was decreased significantly compared with the hyperxia group and hyperxia control group. Compared with the normal control group,higher protein and mRNA expression of MMP?9 were observed in the hy?perxia group and hyperxia control group. The expression of MMP?9 protein and mRNA were decreased in the GM6001 treated group compared with the hyperxia control group(P<0.05). Conclusion The abnormal expression of MMP?9 was closely correlated with RNV. The development of RNV can be markedly inhibited by MMP?9 inhibitor(GM6001),which,we believe,will provide new molecular targets and therapeutic strategy for retinopathy of prematurity treatment.

Objective To investigate the action mechanism of microRNA‐152(miRNA‐152) in the cisplatin(DDP) resistance process in non‐small cell lung cancer (NSCLC) .Methods The miRNA‐152 level in NSCLC cell line A549 and its cisplatin‐resistant cell line A549/DDP was detected by the real time quantitative PCR(qRT‐PCR) .miRNA‐152 mimic was transfected for increasing the intracellular miRNA‐152 level in A549/DDP .The MTT assay ,inverted miroscope technique and flow cytometry were adopted to observe the effect of up‐regulating miRNA‐152 on cell proliferation inhibition and apoptosis ,meanwhile ,the level changes of intra‐cellular Bcl‐2 and NF‐κB were also observed by adopting qRT‐PCR and Western blot .Results The low expression of miRNA‐152 and the high expression of Bcl‐2 and NF‐κB were found in A549/DDP cells .Up‐regulation of miRNA‐152 enhanced the inhibitory effect of DDP in A549/DDP cells .Furthermore ,after up‐regulating miRNA‐152 ,the inhibiting rate and apoptosis rate of A549/DDP cells caused by DDP were significantly higher than those in the cells without up‐regulating miRNA‐152 ,the difference was statisti‐cally significant(P<0 .05) .In addition ,miRNA‐152 mimic transfection significantly decreased the expression of Bcl‐2 and NF‐κB in A549/DDP cells .Conclusion Low expression of miRNA‐152 may induce the resistance of NSCLC to DDP ,miRNA‐152 could medi‐ate the sensitivity of NSCLC cells to DDP via regulating Bcl‐2 and NF‐κB levels .

The development of membrane separation technology in antibioti cs extraction was presented. The application of high performance capillary electr ophoresis, aqueous two-phase system and reverse micelles extraction were also b riefly introduced. Besides, the foreground of these modern separation technolog ies was discussed.

OBJECTIVE:To reduce the types of drugs which was not consistent with the accounts in monthly checking of out-patient department,reduce the wastage of dismounted drugs and improve dismounted drug management. METHODS:Through qual-ity control circle(QCC),analyzing the reasons for inconformity of accounts and drugs,adopting relevant countermeasures of staff training,cipher prescription,counter storage,managed by designated person,expiry data management and so on,the process of dismounted drug management was formulated and implemented. The types of drugs which was not consistent with the accounts and activity growth value of circle members were analyzed statistically before,during and after QCC. RESULTS:The types of drugs which was not consistent with the accounts were 33.6,21.2 and 13.6 before,during and after QCC,decreasing by 59.5%. The ac-tivity growth value of circle members were all positive value. CONCLUSIONS:QCC of outpatient pharmacy can effectively reduce the types of drugs which is not consistent with the accounts in monthly checking,standardize dismounted drug management and im-prove pharmaceutical care quality.

Objective To investigate the safe directions of screw trajectory in atlas via posterior arch and lateral mass and its clinical significance. Methods Lateral radiographs and CT axial scans of atlases were performed in 30 cases with normal morphology of atlases and axes. The minimal height of posterior arch, the maximum inclination of screw projection relative to sagittal plane, and the maximum medial angle of screw projection relative to axial plane were evaluated radiologically. According to the safe directions obtained radiologically 21 cases of atlantoaxial instability were treated with screw fixation atlas via posterior arch and lateral mass. During operation the influence of screws on surrounding structures was investigated and postoperative neural symptoms were documented also. Preoperative and postoperative radiographs and CT scans of 13 patients were available and some related parameters were measured to evaluate the safety of the screw placements. Results 1) The maximum angle of screw projection to sagittal plane is about 10? cephalad to 6? caudal, with the tendency of increasing maximum angle as the minimal height of posterior arch increases. 2) When the entry point on the posterior arch was switched laterally, the medial angle of screw projection should be adjusted from 0? to 30?, correspondingly. 3) The actual directions of screw trajectory might differ from preoperative ones, but all were in the estimation range. 4) All screws were placed successfully, and the postoperative radiographs and CT scans shows no neural or vascular complications relative to atlantal screws placed in traditional way. Conclusion There is a safe range to insert atlas screw via posterior arch and lateral mass both in sagittal and axial plane.

With the development of health care, the use of modern medical devices and the improvement of hospital manage-ment system, Intensive care unit( ICU) emerges.ICU is a treatment-nursing ward, which is a collection of modern medical care tech-nology.With ICU information constructive practice of Nanjing General Hospital of Nanjing Military Region as the background, this pa-per introduces the target base、functional requirements、business processes of the intensive care information system management mode of operation from the perspective of clinical application.Furthermore, this paper describes the realization of the function of the mode of operation with cases as evidence.

Chop and Grp78 genes are key genes in endoplasmic reticulum stress(ERS).To explore the gene alteration of Chop and Grp78 in embryo with spina bifida,we studied Chop and Grp78 gene expression by qRT-PCR in neural tube of rat with spina bifida in E12 and primary cultured neuroepithelial stem cells(NEP).We found higher expression of both Chop and Grp78 in neural tube of rat with spina bifida than normal rats.While Chop and Grp78 genes were up-regulated after NEP differentiated into neuron.The up-regulation of Chop and Grp78 genes in neural rube of rat and NEP might be involved in the abnormal apoptosis in spina bifida related with ERS.And we found the primary culture of NEP provided a good model for embryo early development research.