Aim To study the inhibitory effect of Oxymatrine-Baicailin compound on the secretion of hepatitis B viral antigens in HepG 2.2.2.15 cells.Methods HepG2.2.2.15 cells were cultured and treated with a series of Oxymatrine,Baicailin,or Oxymatrine-Baicailin compound respectively.The toxicity effect was determined by MTT colorimetry.Con-tents of the hepatitis surface antigen(HBsAg) and hepatitis e antigen(HBeAg) in the culture supernatants were determined by Enzyme linked immunosorbent assay.Results Oxymatrine at concentrations between 0.125 and 1 g?L~(-1) had little toxicity effect on cells,but Oxymatrine at 2 g?L~(-1) and 4 g?L~(-1) had much more toxicity effect on cells.The inhibitory effect of Oxymatrine on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1).The toxicity of Baicailin on cells increased from 0.625 to 2 g?L~(-1),especially when the dose surpassed 1 g?L~(-1).The inhibitory effect of Baicailin on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1),but its efficacy was inferior to Oxymatrine's efficacy.Oxymatrine-Baicailin compound had good inhibitory effect on hepatitis B viral antigens secretion,and the inhibition effect of the compound on HBeAg was superior to the effect on HBsAg.The Group C Oxymatrine-Baicailin compound had good synergism inhibition effect on hepatitis B viral antigens secretion and the inhibition rate of the specific group compound was significantly superior to that of Oxymatrine treatment alone(HBsAg:P=0.043;HBeAg: P=0.026).Conclusion Oxymatrine-Baicailin compound has good synergism effect on hepatitis B viral antigens secretion in HepG2.2.2.15 cells.

Animals ; Breast Neoplasms ; pathology ; Cell Division ; physiology ; Cells, Cultured ; Humans ; Macrophages ; cytology ; Signal Transduction ; immunology ; physiology ; Space Flight ; Tumor Cells, Cultured ; Weightlessness

AIM: To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer(MIP) and Scatchard analysis was carried out. METHODS: Pueraria MIP was synthesized using pueraria as the template,acrylamide as the functional monomer and ethylene glycol dimethaerylate(EDMA) as the crosslinker. RESULTS: The result showed that at least two classes of binding sites were formed in the imprinted polymer.Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION: The component of the products is analyzed by HPLC.The result shows that the final products contain pueraria and the other two kinds of isoflavone.

Renal Tubular Cell Apoptosis refers to activation,proliferation,distension,cast formation and cellular apoptosis of the tubular epithelium when the renal tubule undergoes hypoxia,poisoning,inflammatory reaction,plasma protein or amylaceum.This text summarized the researches on the prevention and control of lenal tubular cell apoptosts in renal interstitial fibrosis in the recent years.

Objective To explore the clinical effect of pelvic floor muscle rehabilitation therapy to recover pelvic floor dysfunction.Methods 435 cases of 6-8 weeks postpartum urinary incontinence patients were chosen.The urinary incontinence questionnaire ( ICI-QSF) ,and Female Sexual Function Index questionnaire ( FSFI questionnaire) were used to detect maternal urinary incontinence (UI) investigation,pelvic organ prolapse (POP-Q) degree and quality of sexual life questionnaire.Ⅰ muscle,Ⅱ muscle strength and fatigue, vaginal and the change of dynamic pressure for pelvic floor functional change before and after Pelvic floor rehabilitation were compared.Pelvic floor mus-cle rehabilitation treatment included:imitation bioelectricity stimulus,biofeedback,scene reflection training and Kegel exercises.Results After pelvic floor muscle rehabilitation,pelvic floor function improved significantly,pelvic floor muscle strength increase, decrease fatigue, vaginal dynamic pressure rose significant difference:Electrical values (μV) before treatment (5.6 ±1.8),after treatment(15.1 ±4.6),t =3.6,P <0.05;Vaginal dynamic pressure (cmH2O) before treatment(48.7 ±11.0),after treatment (86.3 ±5.1)cmH2O(t=7.2,P<0.01);Fatigue obvi-ously improved,(P<0.01),the effect of Urinary incontinence treatment is very obvious (t=5.6,P<0.05)and pelvic organ prolapse improved significantly:Before treatment vaginal wall prolapse II degrees 63 people,after treat-ment 14 people,remission rate 82%,and Before treatment uterine prolapse 172 people,after treatment uterine pro-lapse 39 people,the response rate of 77.3% (P <0.01),sexual function improved significantly:before treatment score is (78.00 ±20.45) and after treatment scores is (100.00 ±25.36),t=8.6 (P<0.01).Conclusion Pelvic floor muscle rehabilitation effectively improve postpartum women pelvic floor function,can achieve the purpose of the treatment of urinary incontinence,improve pelvic organ prolapse and the quality of sex life.

This review is to provide an overview of current situation and advance of abdominal compartment syndrome. Progress has been made in diagnosis and therapy of abdominal compartment syndrome. At present patients who are diagnosed as abdominal compartment syndrome are associated with a high mortality rate. Therefove, it is important to diagnoze and treat the disease early. Surgical treatment of increased intraabdominal pressure leads in most instances to a rapid and profound correction of the physiological abnormalities. Operative treatment is the unique and effective approach of abdominal compartment syndrome.

In the paper, a microtiter plate method was used to determine the residual prekallikrein activator(PKA) activity in blood products.The result of study indicated that it would be satisfactory that the reaction mixture of PKA and prekallikrein (PK) was iucul ?? at room temperature for 30 minutes with 0.05M Tris/0.15M NaCl buffer(pH 8.0) , and then the mixture continued to be incubated at room temperature for another 15 minutes after the chromogenic substrate for kallikrein was added. The method had a good reproducibility. The PKA contents determined in IVIG agreed with that of the processing method of IVIG and the hypotensive test of rats, and were basically consistent with the result, determined by the method developed by the Finnish Red Cross Blood Transfusion Service.

Objective To investigate the effect of mytomycin C in the operation of dacryocystorhinostomy for curing lacrimal passage obstruction. Method Forty-one patients with traumatic lacrimal passage obstruction were divided into treatment group (n=21, 14 males and 7 females with an average age of 32) and control group. (n=20, 12 males and 8 females with an average age of 35). The patients in treatment group received 0.4mg/ml of mytomycin C when undergoing dacryocystorhinostomy, while the patients in control group received no medicine when undergoing the same operation. Lacrimal passage was cleaned with water after operation, and the operational effectiveness of dacryocystorhinostomy were compared between the treatment group and control group. Results Twenty-one eyes were cured in treatment group, and the cure rate reached 100%. While for the 20 eyes in control group, 17 eyes were cured, and the cure rate was 85%. There existed statistical significance between the two groups (P

Objective To induce experimental autoimmune myasthenia gravis (EAMG) rabbits by using T?125～147 Methods Peptides T?125～147 were synthesized referring to the residue sequence of acetylcholine receptor of Torpedo California and the rabbits were inoculated with the peptides Clinical manifestation was graded in 4 levels Electrophysiological function was assessed by repetitive nerve stimulation (RNS) and single fibre electromyography (SFEMG) tests Anti peptides antibodies were evaluated by enzyme linked immunosorbent assay (ELISA) Student t test was used to analyze the difference between the EAMG and healthy rabbits Results Following the second inoculation,the rabbits appeared weakness Clinical symptoms were improved by neostigmine At 3,5 and 10 Hz,the decrement of compound muscle action potential (CMAP) and the mean jitter (MCD) of the immunized rabbits were higher than in the healthy ones The percentage of decrement of CMAP in order of the control group and the T?125～147 group were:3 Hz: 1 625?1 317,25 375?7 945; 5 Hz: 2 000?1 732,25 625?9 102; 10 Hz: 1 750?1 392,28 875?8 709.Following the above sequence,the MCD were:3 Hz: (9 875?1 126) ?s,(25 875?7 945) ?s; 5 Hz: (11 375?0 916) ?s,(27 500?3 381) ?s; 10 Hz: (12 375?1 061) ?s,(31 000?4 811) ?s Anti peptide antibodies in immunized groups were significantly higher than those in the control group Conclusion T?125～147 might be served as the immunogenic to induce EAMG in rabbits,accompanied by elevation of anti peptide antibodies and the blockage of neuromuscular transmission

Objective To discuss the diagnosis and clinical value of spiral CT(SCT) in orbital blowout fracture.Methods The SCT MPR findings in conjunction with transaxia images in 120 cases of orbital blowout fracture were retrospectively analyzed. According to the this analysis,108 patients were treated conservatively only 12 were under the opereation.Results Interruption of bone continuity, fragments, depression and displacement of orbital bone wall were direct signs of orbital blowout fracture." Tear drop"sign in the maxillary or ethmoid sinus was a characteristic indirect sign of inferior or medial orbital wall fracture. Enophthalmos obviously compacting of extraocular muscler and distraction test male were operation signs.Conclusion Orbital blowout fracture can be diagnosed by SCT volume scanning findings of the MPR comprehensively and accurately, that is very important in choosing the treatment methods for orbital blowout fracture.