Objective To investigate the effects of different interventions on the biomechanic indexes in femur bones of ovariectomized rats with osteoporosis.Methods Eighty healthy female Sprague-Dawley rats,aged 3 months,were randomly divided into an ovariectornized(OVX,n=68)group and a sham-operation(Sham,n=12)group.At the 11th week after the operation,the OVX group was further randomly divided into a control group(n=11),an estradiol group(E2,n=10),a genisteine group(G,n=10),a treadmill exercise group(TE,n=10),a lithium chloride group(LiCl,n=10)and a whole-body vertical vibration group (WBVV,n=10).Then the rats began to receive different interventions as their group names implied.At the end of 8 weeks,their right femurs were isolated and the biomechanic parameters were detected using the three point bending test.Results (1)Both the maximum load and elastic load in E2,G,TE,WBVV and LiC1 groups were significantly higher than that of the control group,while no significant differences were seen in maximum deflection and elastic deflection.(2)The elastic stress,maximum strain,and elasticity modulus in E2,G,and WBVV groups were significantly higher than that of the control group,while no significant differences were seen in maximum stress and elastic strain among them.The maximum stress,elastic stress,maximum strain,and elasticity modulus in TE group were significantly higher than the control group while no significant differences were seen in the elastic strain.The maximum stress,elastic stress,and elasticity modulus in LiC1 group were significantly higher than the control group,but no significant differences were seen in maximum strain and elastic strain between them.Conclusion E2,genistein,treadmill exercise,whole-body vertical vibration,and LiC1 have quite similar effects on structural mechanics indexes of femurs in OVX rats with osteoporosis,but they have different impacts on the material mechanics indexes.

Objective To investigate the effect of 5-FU and Lobaplatin in the treatment of advanced hepatocellular carcinoma in serum AFP and TIP30.Methods 86 cases of advanced hepatocellular carcinoma in our hospital form June 2014 to June 2016 were selected and randomly divided into two groups,43 cases in the control group were treated with micro catheter perfusion 5-FU sequential therapy,43 cases in the experimental group were treated more with Lobaplatin sequential therapy.The serum levels of AFP, AFP-L3, Fer, TSGF, TIP30, clinical efficacy and adverse reactions were compared before and after treatment in the two groups.Results Compared with before treatment, levels of AFP-L3,Fer,TSGF and AFP decreased in two groups, levels of TIP30 increased (P<0.05);compared with the control group,levels of AFP-L3,Fer,TSGFand AFP in the experimental group were lower(P<0.05),and levels of TIP30 were higher(P<0.05),and the total efficiency of the experimental group was higher than the control group(P<0.05),and the incidence of adverse reactions in the experimental group was lower than the control group(P<0.05).Conclusion 5-FU and Lobaplatin in the treatment of advanced hepatocellular carcinoman can effective reduce the levels of AFP,AFP-L3,Fer,TSGF,and increased the levels of TIP30.

Breast Neoplasms ; diagnosis ; genetics ; metabolism ; Carcinoma, Intraductal, Noninfiltrating ; genetics ; metabolism ; Chromosomes, Human, Pair 17 ; genetics ; Female ; Genetic Heterogeneity ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Neoplasm Metastasis ; Neoplasm Recurrence, Local ; Polyploidy ; Receptor, ErbB-2 ; genetics ; metabolism

Fecal isolates of Escherichia coli which were collected from diarrheal patients and HUS patient in Pusan National University Hospital between 1990 and 1996, were serotyped and analyzed for plasmid DNA profile, biotype, HEp-2 cell adherence ability, reactivity to eae probe and for production of verotoxins (VT). In order to ease the diagnosis of EHEC infection, a LPS- based solid phase enzyme linked immunosorbent assay was utilized to detect serological diagnosis of EHEC infection. The following results were obtained. Among 150 EPEC isolates and HUS patient's stool, 7 EHEC were found. The 7 EHEC belonged to 5 different serotypes 0157:H7, 0143:H-, 0166:H-, 0128:H2, 026:H-, and 0111:H 21 previously associated with human haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). Biochemical cheracteristic analysis indicated 7 strains were biotype 1 and was found to have siderophilins suggesting their advantagous growth in vivo. For plasmid profiles, all strains had 60 MDa plasmid and several smaller sizes of plasmids. Three strains of Escherichia coli serotype 0157:H7, 0128:H2, and 026:H- showed one pattern of adherence in the HEp-2 cell assay namely, localized adherence and were positive for eae probe when tested by colony blot hybridization assay. PCR using specific primers for VT1, VT2 was tested, and all 7 strains carried VT1 gene only. PCR products of 130-bp (VT1) and 346-bp (VT2) were successfully amplified simultaneously in a single reaction. The multiplex PCR method can be used to specifically identify EHEC. The serum obtained from HUS patient of enterohemorrhagic E. coli were analyzed for rises in titer of intibody to somatic 02, 026, 0111, 0128, 0143, 0145, 0157, and 0165. Although response to the somatic 0 correlated significantly with response to the 026 rises of antibody titer to somatic 0 in acute stage of disease and anti-VT had not so many relation to that of VT. These results suggest that ELISA can be used to detect somatic 0 in serum and it is a useful method to diagnose the infection caused by EHEC rapidly.
Antibodies* ; Busan ; Colitis ; Diagnosis* ; DNA ; Enterohemorrhagic Escherichia coli* ; Enteropathogenic Escherichia coli ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; Humans ; Multiplex Polymerase Chain Reaction ; Plasmids ; Polymerase Chain Reaction* ; Shiga Toxins ; Transferrin

Three hundred and thirty eight patients with type 2 diabetes mellitus( DM) admitted from January 2006 to June 2009 and 50 healthy subjects (control group) were enrolled in the study. Platelet glycoprotein PAC-1 and CD62P levels were measured in both groups. Compared with control group, plasma PAC-1 and CD62P levels of DM patients increased significantly (P ＜0.01). Patients were divided into three groups according to their urinary albumin excretion rate ( UAER) . PAC-1 and CD62P levels in macroalbuminuria group (UAER≥200μg/min) were significantly higher than those in normoalbuminuria group( UAER <20μg/min) and microalbuminuria group (20μg/min≤UAER ＜ 200μg/min) (both P＜ 0.05). The results indicate that plasma levels of PAC-1 and CD62P, which reflect platelet activation, are the independent risk factors for diabetic nephropathy.

Breast Neoplasms ; pathology ; Carcinoma, Ductal, Breast ; pathology ; Humans ; Medical Records ; standards

Ethanol ; Fixatives ; Formaldehyde ; Humans ; Immunohistochemistry ; methods ; Time Factors ; Tissue Fixation ; methods ; standards

Objective To evaluate the feasibility of testing the high methylation of ppENK gene in stool with methylation-specific polymerase chain reaction (MSP) assay in pancreatic cancer diagnosis.Methods Twenty-four fresh stool samples of pancreatic cancer patients and six healthy control samples were collected.The methylation status of ppENK gene in all the stool samples was detected by MSP assay.The positive rate of wild-type ppENK gene in all the stool samples was determined by polymerase chain reaction (PCR).And 10 non wild-type ppENK gene negative pancreatic cancer samples were collected,and K-ras gene mutation was detected by PCR-restriction fragment length polymorphism (RFLP).The single cell suspension of pancreatic cancer PC3 cell line was added into stool sample from the same healthy individual,the positive rate of ppENK gene methylation was detected by MSP assay.The minimum number of pancreatic cancer cell was calculated when methylation was positive.Results The rate of methylation detection in 30 samples was 0 (0/30); and the rate of non-methylation detection was 10％ (3/30).The rate of wild-type ppENK detection was 6.7％ (2/30).By PCR-RFLP assay,eight were successfully amplified and seven had mutation in 12th code of K-ras gene in 10 selected wild-type ppENK gene negative pancreatic cancer samples.The minimum number of pancreatic cancer cells needed for ppENK methylation band positive detected by MSP was 50 cell/ml.Conclusion Detecting ppENK gene methylation status in stool samples of pancreatic cancer patients by MSP assay has not yet become the method of pancreatic cancer screening and diagnosis.

This article introduces the concept of organizational citizenship behavior(OCB) as well as the idea of medical professionalism,and analyzes the mutual relationship between OCB and medical professionalism.On the basis of introduction and comparison,medical professionalism obtains the enlightenment from previous studies on OCB.

Aiming at the disadvantages of traditional medical high value consumable management mode, bar code management is the only way which must be passed to the high value consumables in hospital management under the new situation. The bar code management system development in theMilitary Medical Projectmaterials management platform, scientific optimization of high value consumables management process, fully embodies the characteristics and advantages of bar code management. Not only to achieve a one relation between the high value consumables and patients, can also be integrated management supplies out of storage, charging and the financial sector, to achieve high value. The bar code in the ID, is the only identity code high value consumables, which maintained the patient's vital interests, and ensure that the hospital infection control requirements, the high value consumables in hospital benigndynamicmanagement system.