[ABSTRACT]AIM:Toexploretheeffectsofchrysinoninsulinresistance(IRe)inamousemodel.METHODS:Male C57 mice were randomly divided into control group , IRe group, low-dose chrysin group ( IRe+chrysin-low) and high-dose chrysin group (IRe+chrysin-high).After 24 weeks, the body weight, liver index and fat mass in all mice were detected.The blood glucose , insulin level and HOMA-IR were measured to determine the changes of the insulin resistance in the animals.The oxidative stress (SOD, GSH-Px and MDA) was also measured.The mRNA expression of insulin sig-naling pathway molecules (IR, IRS1, IRS2, Glut2 and Glut4) and inflammatory factors (TNF-α, IL-1β, IL-6 and NF-κB) was analyzed by real-time PCR.The protein levels of IRS1 and p65, and their phosphorylation were detected by West-ern blot.RESULTS:After 24-week intervention , the indicators in IRe group were higher than those in control group , in-cluding body fat deposition, serum glucose, serum insulin, HOMA-IR and liver oxidative stress (P<0.01), indicating that the model of insulin resistance was successfully established .Low dose and high dose of chrysin decreased the body weight, serum glucose, serum insulin and HOMA-IR in the IRe mice (P<0.05).The liver oxidative stress was also re-duced in both groups (P<0.05).However, no statistical difference of the indexes between IRe +chrysin-low group and IRe+chrysin-high group was observed.Chrysin upregulated the mRNA expression of IR , IRS1, IRS2, Glut2 and Glut4 (P<0.05), and down-regulated the mRNA expression of various inflammatory factors .The inhibitory effect of chrysin on the mRNA expression of NF-κB was observed (P<0.05), especially in high dose group (P<0.05).It was confirmed that the effect of chrysin on liver IRe was related with the increase in the p-IRS1 levels and decrease in the p-p65 levels by Western blot .CONCLUSION:Chrysin inhibits obesity , hyperglycemia and hyperinsulinemia , and relieves insulin resist-ance and oxidative stress , which might be closely related to the regulation of insulin signaling pathway and the inhibition of inflammatory factor expression .

Transcranial doppler (TCD) was used to measure the flow velocities of middle cerebrial artery (MCA) in 4 patients of clinical brain death. The reaults showed that the changes were similar in all the patients studied; 1) the dierotic notch (DN) was deeper in the spectrum of flow velocities of MCA, end diastolic flow rate (VD) significantly decreased, and the pulastility index (PI) increased ; 2) VD decreased progressively; 3) all patients showed characteristic to-and-fro TCD pattren with antegrade flow in systole and retrograde flow in diastole; 4) the pattern totally disappeared when the patients were claimed dead. It is found that TCD is valuable for the determination of brain death.

Objective To discuss the application of core needle aspiration biopsy to diagnosis of breast csncer. Methods 64 cases with breast cancer tumor were checked byCNB and compared with surgical pathology results.Then the method of CNB operation technology was analyzed. Results The diagnostic sensitivity was 96.0% and specificity was 100%. There was no complication during biopsy. The false negative raty was 4.0%. Conclusion The application of core needle aspiration biopsy was simple, safe and reliable. And it could improve the diagnosis of breast cancer.

Objective To discuss the influence of clean-up frequency of the operative cavity on the curative effect after endoscopic sinus surgery.Methods 368 patients with sinusitis or nasal polyps were randomly divided into observation group and control group,184 patients in each group.Control group was treated with operative cavity cleaning in moderate frequency,while observation group in intensive frequency.Results The obvious effective rate of observation group was 80.98％,which was significantly higher than that of control group(69.02％) (x2 =12.468,P ＜0.05).The ineffective rate of observation group was 2.17％,which was significantly lower than that of control group (9.78％) (x2 =15.717,P ＜ 0.05).The recurrence rate of observation group was 11.96％,which was significantly lower than that of control group (26.09％) (x2 =9.361,P ＜ 0.05).Conclusion The clean-up frequency of the operative cavity after endoscopic sinus surgery can influence curative effect,moderate clean-up frequency can significantly improve the treatment effects and deserved promotion.

Objective:Toinvestigate the influence of MMP7 antisense oligodeoxynucleotide(ASODN)on adhesion and invasion ofhuman lung adenocarcinoma cell line A549.Methods:Phosphorothioate MMP7 ASODN was transfected to A549 cells mediated by liposome. The expression of MMP7 was examined by RT-PCR.The adhesive and invasive ability were examined by plate adhesion model and Boyden Chamber transwell assay.Results:After MMP7 ASODN was transfected,the relative optical density(ROD)of electrophoresis strip was decreased obviously(P

Objective To analyze the pathophysiological variation in patients with penetrating cardiac trauma (PCT) and discuss the classified treatment strategies.Methods A retrospective analysis was conducted on pathogenetic condition,medical interventions and other clinical information of 133 PCT patients.The patients were divided into four groups in accordance with their symptom classification on arrival at emergency department,including sub-clinic type,cardiac tamponade type,hemorrhagic shock type and agonal type.Meanwhile,the patients were classified into three groups according to their pathogenesis phase,including sub-clinical phase,clinical phase and agonal phase.Development characteristic of patients' condition in each group was investigated in each time point by survival analysis.Impacts of medical interventions on prognosis of those patients were analyzed,such as pericardial space exploration,emergency room thoracotomy (ERT),operating room thoracotomy (ORT).Results Some patients at sub-clinical phase were aggravated into clinical phase or agonal phase,as well as some patients at clinical phase were aggravated into agonal phase during in-hospital treatment.There were significance differences of posttraumatic suvival course among the four groups,namely sub-clinical type,cardiac tamponade type,hemorrhagic shock type and agonal type (P ＜ 0.01).The differences of posttraumatic survival course were also significant among the three groups,namely sub-clinical phase,clinical phase and agonal phase (P ＜ 0.01).Conclusion Clinical symptom classification (or pathogenesis phase) of PCT may not be always unchangeable,thus it is recommended that PCT patients should be treated based on their clinical symptom classification or patbogenesis phase at consultation.

No abstract available.
Organ Transplantation* ; Transplants*

Objective To discuss the clinical curative effect of laparoscopic totally extraperitoneal hernia repair spermatic vein high ligation in the treatment of varicocele.Methods 100 cases with varicocele were selected as the research subjects.All patients with clinical symptoms and color Doppler ultrasound diagnosis of varicocele and were randomly divided into observation group and control group,50 cases in each group.The control group was given the laparoscopic through abdominal cavity retroperitoneal spermatic vein high ligation,and the observation group was given the laparoscopic totally extraperitoneal hernia repair spermatic vein high ligation.Two groups were given the same nursing intervention and support treatment in perioperation.General clinical indicators, treatment effect and postoperative complications of the two groups were compared.Results The surgery time[(17.5 ±2.4)min],length of hospital stay[(48.5 ±0.1)h]of the observation group had no statistically significant differences compared with the control group[(18.2 ±3.1)h,(48.1 ±11.5)h](P>0.05).The intestinal peristalsis time[(5.2 ±1.2)h]and ambulation time[(10.5 ±2.4)h]of the observation group were significantly shorter than (7.5 ±2.0)h,(15.7 ± 3.3)h of the control group,the differences were statistically significant (t=1.731,1.925,all P<0.05).Incidence of postoperative complications had no significant difference between the two groups,while improvement rate of semen of the observation group ( 98%) was obviously higher than 84% of the control group, the difference was statistically significant (χ2 =0.587,P<0.05).Conclusion Laparocopic totally extraperitoneal hernia repair spermatic vein high ligation in the treatment of varicocele has exact curative effect,has less effect on abdominal cavity,faster recovery of digestive function and postoperative rehabilitation, ideal semen improvement, which has clinical application and popularization value.

[Summary] As of 2014, an estimated 387 million people have diabetes mellitus ( DM) worldwide, which represents 8.3%of the adult population.China Noncommunicable Disease Surveillance in 2010 shows that the overall prevalence of DM is estimated to be 11.6%(approximately 113.9 million) in the Chinese adult population, with the prevalence among men of 12.1%and women of 11.0%, respectively.Control of blood glucose is fundamental to DM management.Despite the availability of several antihyperglycemic agents, only 53%of patients with DM achieve the recommended goals for DM care of HbA1C<7.0%.According to the National Health and Nutrition Examination Survey and the Behavioral Risk Factor Surveillance System Survey during the period of 10 years in the United States, 33.4%to 48.7%of persons with DM still have not met the targets for glycemic control, blood pressure or lipid level.In order to improve glycemic control, there is a need for new therapeutic options with innovative mechanisms of action and acceptable safety profiles.As a newly developed class of oral antidiabetic drugs, sodium-glucose co-transporter 2 inhibitors(SGLT2i) have recently been approved for the treatment of patients with type 2 diabetes mellitus, including canagliflozin, dapagliflozin, and empagliflozin.Evidence from clinical trials has suggested promising efficacy and safety of SGLT2i when used as monotherapy or in combination with other antihyperglycemic medications.SGLT2i significantly reduce HbA1C and fasting plasma glucose, and are well tolerated in general with a low intrinsic propensity to cause hypoglycemia, as well as rare severe renal or cardiovascular adverse events reported.

Purpose:To study LPS induced iNOS mRNA expression and NO production by murine peritoneal macrophages, and their relationship with cytotoxic function of activated macrophages.Methods:LPS induced iNOS mRNA expression, production of NO and effects of activated macrophages on anti tumor were investigated separately by RT PCR, nitrate reductase method and 3HTdR incorporation.Results:LPS can upregulates iNOS mRNA expression in a dose dependent manner. L NIL(specific inhibitors of iNOS) pretreatment significantly reduced cytotoxic effect on the proliferation of HL 60 cells, but had little effect on the K562 tumor target cells on condition that NO production is sufficiently inhibited. Conclusions:LPS can effectively induce iNOS mRNA expression and NO production in murine peritoneal macrophages. NO is one of the important effector molecules in unspecific immunity of activated macrophages, but it plays different roles in cytotoxic effects of macrophages on different target tumor cells.