Objective:To compare the scanning electron microscopic changes of venous endothelium after acute inferior vein thrombosis in rats.Methods:The inferior caval venous thrombosis models were treated with heparin(n=35,group A),urokinase(n=35,group B),and combination of heparin and urokinase(n=35,group C),respectively.Sham group(n=30)was also established.The thrombosed cav-al veins were taken 1,4,7,14,and 28 days after thrombosis.Scanning electron microscopy was used to observe the damage of endothelial cells.Results:The Results showed that the endotheliurn injury degree was mainlyⅡtoⅢgrade in group B and C whereas it wasⅢtoⅣ grade in group A on day7(P

Objective:To investigate the diagnostic value of MRI in the knee anterior cruciate ligament tear.Methods: retrospective analysis for treatment of 40 patients with anterior cruciate ligament tear by observation group, by analyzing the clinical data and image, and selected the same period for treatment of 20 cases of patients with knee joint other lesions but ACL normal MRI data comparison by Control group, The form characteristics of ACL and abnormal signal strength, whether there is a line or an ACL tear with signs were analyzed.Results:The MRI diagnosis of ACL tear completely is higher for the sensitivity and specificity (87.5%, 90%; Diagnosis of ACL partial tear, and arthroscopy and surgery results in 8 cases, the rest 4 cases of complete tear, 4 cases of false negative; ACL tear with signs including abnormal curvature of bone contusion, photo, or meniscus has obvious lateral collateral ligament damage, tibia forward, etc. ACL tear group and normal group average photo Angle, PCLS L/H mean significant difference.Conclusion: through analysis of MRI diagnosis torn ACL completeness, has high accuracy, but the part in the ACL tear or chronic tear diagnosis, accurate rate is relatively low.

Objective To investigate the effects of sevoflurane preconditioning on cardiovascular function in patients with coro -nary heart diseases , and explore its mechanism .Methods Eighty patients with coronary heart disease during 2009 January to 2012 December from our hospital patients were selected and randomly divided into the study group ( 41 cases ) and control group ( 39 ca-ses) .Patients in study group were given sevoflurane preconditioning , while the control group were given propofol .A series of parame-ters were recorded, including the patient's heart rate (HR), central venous pressure (CVP), mean arterial pressure (MAP), stroke volume (SV), cardiac output (CO), systolic time ratio (STR) and serum cardiac troponin T (cTnT) before induction of anesthesia (T0), 30min (T1) during the operation, incision suture (T2), 6h after operation (T3), 12h after operation (T4), and 24 h after op-eration (T5).Results HR, CO, SV, CVP, MAP, STR, and cTnT of the research group were relatively more stable than the control group in the preoperative, intraoperative, postoperative, and recovery.The study group at CO, T1, SV, STR, and cTnT were (3.48 ±0.40)L/min, (6.4 ±1.9)ml/(min· m2), (0.36 ±0.76), (0.227 ±0.112)ng/ml, CO, SV, STR in T2 and cTnT were (3.58 ±0.52)L/min, (6.6 ±2.3)ml/(min· m2), (0.36 ±0.63), (0.241 ±0.115)ng/ml, control group T1 at CO, SV, STR and cTnT were (3.11 ±0.53)L/min, (5.2 ±2.1)ml/(min· m2), (0.46 ±0.81), (0.351 ±0.106)ng/ml, CO, SV, STR in T2 and cTnT were (3.15 ±0.61)L/min, (5.7 ±1.5)ml/(min· m2), (0.44 ±0.90), (0.311 ±0.112) ng/ml.The patients in study group were in T1 CO, SV CO in T1 , SV, T2 times higher than that in control group .STR in T1 , T2 point was lower than that of the control group, cTnT in T1 , T2 point was lower than that of the control group , and the difference between the groups was statistically significant ( P <0.05 ) .Research group of patients with CO , SV higher than that in control group at in T 1 ,T2 , and STR lower than that at T 1 , T2 , cTnT lower than that of the control group at in T 1 , T2 , and the differences were statistically significant ( P <0.05 ) .Conclusions Sevoflurane preconditioning can make the hemodynamics more stable , more suitable for clinical anesthesia , and its mechanism may be related to the changes of cardiac troponin T .

OBJECTIVE:To establish a method for qualitative identification of Shenjin huoxue mixture.METHODS:Ly-copodii japonicum,Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae in the preparation were identified by TLC.RESULTS:At the corresponding positions,the color of TLC spots of Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae in the test samples and in reference substances were identical.There was no interference form negative control.But no characteristic spots of Lycopodii japonicum in the sample were noted.CONCLUSION:The established TLC identification method of Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae is reproducible,specific,reliable and suitable for the quality control of Shenjin huoxue mixture.The absence of characteristic TLC spots of Lycopodii japonicum in the sample is suggestive of the necessity of improving the production technology of Shenjin huoxue mixture.

DNT cells(CD4-CD8-double-negative T cells)ale newly discovered immune cells,whose surface ex press neither CD4 nor CD8 molecules.In recent years,the prognosis and survival quality of tumour patient have been greatly improved through cell immunotherapy.Immunocyte can kill the tumour directly Or through immunoregu lation.The study of DNT cells(double-nnegative T cell)on cancer cells will be of vital significance.

Depression is a worldwide neuropsychiatric disorder. Currently most preclinical and clinical studies of depression focus on monoaminergic system. However, there is growing evidence which suggests that glutamatergic system plays a critical role in the pathophysiology of depression. This review focuses on the de-velopment of new antidepressants that target glutamatergic sys-tem, summarizes the current mechanisms of antidepressants, and also highlights new insights to the pathophysiology of depression.

Objective:To explore the correlation among serum uric acid (UA) level and severity of coronary heart disease (CHD) ,adverse cardiovascular events .Methods :Clinical data of 403 patients undergoing coronary angiog-raphy in our hospital were retrospectively analyzed .They were divided into CHD group [n=308 ,including 137 cases with stable angina pectoris (SAP) and 171 cases with acute coronary syndrome (ACS)] and normal control group (n=95 ,non-CHD patients) .UA level was compared among all groups and its correlation with CHD severity was fur-ther analyzed .According to UA level ,CHD patients were divided into low UA group (n=147) and high UA group (n=161) ,after 12-month follow-up ,incidence rate of major adverse cardiovascular events (MACE) uring follow-up was compared between two groups .Results:Serum UA level of ACS group was significantly higher than that of nor-mal control group [ (318.44 ± 69.07)μmol/L vs .(295.38 ± 80.08)μmol/L ,P=0.003];along with number of dis-eased coronary vessels increased and CHD severity aggravated (Gensini score rose ) ,serum UA level showed an in-creasing trend [single-branch (316.58 ± 95.27 ) μmol/L vs . double-branch (335.26 ± 43.26 ) μmol/L vs . multi-branch (346.53 ± 86.74) μmol/L ;low score group (312.42 ± 48.26) μmol/L vs .middle score group (346.58 ± 47.36)μmol/L vs .high score group (363.84 ± 54.68)μmol/L , P<0.05 or <0.01] .Pearson correlation analysis indicated that serum UA level was positively correlated with Gensini score (r=0.583 ,P<0.05);MACE incidence rate in high UA group was significantly higher than that of low UA group (58.38% vs .36.24% , P=0.012) .Con-clusion:Serum uric acid level is positively correlated with severity of coronary heart disease ,which may be an inde-pendent risk factor for coronary heart disease .

Objective To retrospectively study histopathologic features of ulcerous colitis (UC) and Crohn's disease (CD) by endoscopic biopsy. Methods Data of age, gender and intestinal involvement of 106 cases of CD and 180 cases of UC confirmed by pathology were reviewed. Mucosal architecture, chronic inflammation, acute inflammation and epithelial features were evaluated based on histopathological criteria.These features were compared between the two groups. Results Compared with CD cases, UC showed significantly higher percentage of architectural disorders ( P ＜ 0. 05 ), lower percentage of discontinuous chronic inflammation ( P ＜ 0. 05 ) and higher incidence of cryptitis, crypt abscesses and polymorph cells in lamina propria (P ＜0. 05 ), and higher rate of epithelium damage, flattened and less mucous cell as well. Granuloma, pseudo pylorus glands metaplasia and narrow ulcer only occurred in CD cases. Lesions of 90% (162/180) cases of UC were limited to colon, while those of 28% (30/106) of CD cases to ileocecum, and those of 56% (59/106) CD cases involved different parts of gastrointestinal tract. Conclusion Diagnosis of CD and UC needs comprehensive analysis. Diagnostic evidence for CD includes focal involvement of ileocecum, the multifocal involvement in gastrointestinal tract, granuloma, discontinuous inflammation, pyloric gland metaplasia, while that for UC includes mucosal damage limited to colon, continuous chronic inflammation, architectural disorders, or epithelial damage.

Objective To establish an animal model of open craniocerebral injury with seawater immersion and to study the characteristics of the injury. Methods A total of 100 healthy ma! SD rats were divided into 5 groups: control group, mild injury + seawater immersion group, moderate injury + seawater immersion group, severe injury + seawater immersion group and severe injury + normal saline immersion group. After SD rats had received a controlled cortical impact (CCI) injury, the injured brain tissue was soaked in seawater or normal saline to construct an animal model of open craniocerebral injury. We then observed the characteristics of the model, including the animal behavior, pathological changes, brain water content and the levll of blood brain barrier (BBB) opening. Results The animal model could well simulate the characteristics of the open craniocerebral injury with seawater immersion. With the increase of brain injury severity, the cerebral dysfunction, cerebral pathology, cerebral water content, and the damage of BBB became more serious. The pathological changes of brain tissue necrosis and bleeding in severe injury + seawater immersion group were more nota Me than those in the severe injury + normal saline immersion group. Compared with the severe injury + saline immersion group, the water content of brain tissue and the damage of BBB were significantly increased in the severe injury+ seawater immersion group after immersion for 24 h. Conclusion The rat model we established in this study can simulate the open craniocerebral injury with seawater immersion.

Objective To study the therapeutic method and effects of spontaneous cerebral hemorrhage broken into ventricles of brain.Methods 40 cases of cerebral hemorrhage broken into ventricles of brain were treated by only puncturing and draining the hematoma,unilateral or bilateral lateral ventricle and the hematoma plus lateral ventricle,and the urokinase and discharging the cerebrospinal fluid by lumbar puncture were used after operation.Results The treatment of 26 cases(65%) had notable progress,6(14%) had progress and 2(5%) had no progress or exacerbation and 6 cases(15%) were dead.Tota leffective rate was 80%.Conclusion The methods used in this study are simple,practical and have definitive curative effects.