Objective: To investigate the feasibility of culturing and identifying the cementoblasts in vitro and to study the biological characteristics of rat cementoblasts.Methods: Osteoblasts, PDL fibroblasts and cementoblasts were obtained from alveolar bone and the root surface of rat first molars and cultured with a 2-step method of enzyme digestion/explant technique. Bone osteocalcin(OCN) and alkaline phosphatase(ALP) were detected by quantitative immunohistochemical examination. Alizarin Red S was used to examine the formation of calcified nodules under conditional culture. Results: Primarily cultured cells could be subcultured. Osteoblasts were identified by the expression of OCN and ALP at heigh level, cementoblasts by OCN , PDL fibroblasts by ALP at moderate level, cell clusters/nodules of cementoblasts were observed as the cells approached confluence.Conclusion: Cementoblast-like cells may be cultured in vitro.

BACKGROUND:Posterior antebrachial skin is beneficial for repairing injury of dorsum,however,the systematic anatomic characteristics of distally based compound flap pedicled with nutrient vessels need to be further studied.OBJECTIVE:To investigate the anatomic characteristics of distally based flap pedicled with nutrient vessels of the posterior antebrachial cutaneous nerve and compound flap.DESIGN:Single sample observation.SETTING:Research Center for Clinical Anatomy,Military Institute of Orthopaedics,Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.MATERIALS:The experiment was carried out at the laboratory for Research Center for Clinical Anatomy,Military Institute of Orthopaedics,Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from October to December 2004.A total of 30 upper limbs of adult cadavers perfused with red latex through arteries were provided by Research Center for Clinical Anatomy,Military Institute of Orthopaedics,Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.METHODS:All 30 adults upper limbs were dissected and observed under the microscope based on the pivot point of styloid process of ulna and radius.The following microdissection was emphasized:The origins,branches,distribution and anastomosis of nutrient vessels of the posterior antebrachial cutaneous nerve;The blood supply relationship of nutrient vessels of posterior antebrachial cutaneous nerve and vicinal bone and skin.MAIN OUTCOME MEASURES:The ordgins and branches of nutrient vessels of posterior antebrachial cutaneous nerve,and whose blood supply relationship with vicinal bone and skin.RESULTS:The origin of nutrient vessels of posterior antebrachial cutaneous nerve were as follows:Proximal part:2-6cutaneous perforators of radial collateral artery,with an outer diameter of (0.6±0.3) mm;Distal part:3-5 cutaneous perforators of dorsal carpal branch of anterior interosseous artery,with an outer diameter of (0.8±0.2) mm;Others:6-9cutaneous perforators of posterior nterosseous artery,with an outer diameter of (0.7±0.3) mm.Posterior interosseous artery sent out 6-8 muscle- periosteum branches with an outer diameter of (0.3-1.0) mm,which distribute on ulnar periosteum;Radial bone-skin perforators of posterior interosseous artery anastomosed with periosteal vessels of mid-inferior bare area of radial bone.All above mentioned cutaneous perforators gave off cutaneous branches,fascia branches,periosteal branches and nerve-nutrient vascular branches,all of which formed a vascular chain of cutaneous nervous stem and vascular networks of deep fascia,superficial fascia and periosteum.CONCLUSION:The nutrient vessels of posterior antebrachial cutaneous nerve have the same origins as the vicinal bone-skin nutrient vessels,and the rotation point of its distally based pedicled flap and compound flap can reach the plane of wrist joint,which may be helpful to design a kind of more convenient and simplified flap to repair the distal tissue defects of the hand.

BACKGROUND: Flap pedicled with nutritional vessels of the cutaneous antebrachial medialis nerve is beneficial for repairing injury of dorsum of hand; but it needs the support from systemic anatomic research on distally based compound flap.OBJECTIVE: To study the anatomic theory of the distally based compound flap pedicled with nutrient vessels of the cutaneous antebrachial medialis nerve -basilic vein.DESIGN: Single sample observation.SETTING: Research Center for Clinical Anatomy, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.MATERIALS: The experiment was carried out at the laboratory for Research Center for Clinical Anatomy, Military Institute of Orthopaedics, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from March to August 2003. A total of 30 upper limbs of adult cadavers perfused with red latex through arteries were provided by Research Center for Clinical Anatomy, Military Institute of Orthopaedics, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA. Each cadaver was injected with a mixture of red gelatin to show the branches, distributions and anastomosis of the lesser vessels on the stained small arteries of the finger tips.METHODS: All 30 adult upper limbs were dissected and observed under the microscope based on the pivot point of styloid process of ulna: ①The origins, branches, distribution and anastomosis of nutrient vessels of the inferior 1/3 segment of medial antebrachial cutaneous nerve-basilic vein, and whose blood supply relationship with ulnar periosteal vessels. ②The superficial and deep communicating branches of basilic vein.MAIN OUTCOME MEASURES: ①The origins, branches, distribution and anastomosis of nutrient vessels of medial antebrachial cutaneous nerve, and whose blood supply relationship with vicinal bone and skin. ② The superficial and deep communicating branches of basilic vein.RESULTS: ①The origins of nutrient vessels supplying the inferior 1/3 segment of the lateral antebrachial cutaneous nerve were 5-9 cutaneous branches of ulnar artery with an outer diameter of (0.7±0.3)mm. The ascending and descending branches of wrist superior branches sent out several cutaneous branches nourishing basilic vein, with an outer diameter of (0.6±0.3) mm. Between the position of (6.0-16.0) cm superior to ulnar malleolus, anterior interosseous artery sent out 2-3 perforating bone-skin branches, with an outer diameter of (0.7±0.2) mm; Posterior interosseous artery sent out 1-3 bone-skin perforating branches, with an outer diameter of (0.6±0.3) mm. Both of them were distributed on periosteum and skin of the corresponding area. All above-mentioned cutaneous perforators gave off cutaneous branches, fascia branches, periosteal branches and nutrient vascular branches of cutaneous nerve-superficial vein, and a vessel chain of cutaneous nerve-basilic vein and vascular networks of deep fascia, superficial fascia and periosteum formed. ②Ulnar artery had two accompanying veins, one was grossus, and the other thin. The two veins accompanied along two sides of artery, and the accompanying vein of arterial cutaneous branches infused. Superior wrist cutaneous branches of ulnar artery had two accompanying veins. One pumped into ulnar branch of ulnar vein, and the other one passed through deep fascia, then entered subcutaneous tissue, and straightly pumped into basilic vein. The injected position was (2.3±0.8) cm superior to ulnar malleolus, with an outer diameter of (2.8±0.5) mm.CONCLUSION: The nutrient vessels of medial antebrachial cutaneous nerve-basilic vein and the nutrient vessels of bone and skin are homologous. The rotation point of their distally based pedicled flap and compound flap can reach the plane of wrist joint, which can be applied to repair the distal tissue defects of the hand.

BACKGROUND: Flap pedicled with nutritional vessels of the lateral antebrachial cutaneous nerve is beneficial for repairing injury of dorsum; however, lacks of system researches of the lateral antebrachial cutaneous nerve compound flap.OBJECTIVE: To study the anatomic theory of the distally based compound flap pedicled with nutrient vessels of the lateral antebrachial cutaneous nerve - cephalic vein.DESIGN: A single sample observation.SETTING: Researching Center of Clinical Anatomy, Military Institute of Orthopaedics, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.MATERIALS: The experiment was carried out at the Researching Center of Clinical Anatomy, Military Institute of Orthopaedics, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from January to May in 2004.A total of 30 upper limbs of adult cadavers perfused with red latex through arteries were provided by the Researching Center of Clinical Anatomy, Military Institute of Orthopaedics, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.METHODS: The radial styloid process was taken as the mark for the microanatomical observation of the follows: ① the origins, branches, distribution and anastomosis of nutrient vessels of the inferior 1/3 segment of lateral antebrachial cutaneous nerve-cephalic vein; ② the relationship of nutrient vessels of lateral antebrachial cutaneous nerve-cephalic vein and radius periosteal vessels; ③ the superficial-deep communicating branches of cephalic vein.MAIN OUTCOME MEASURES: ① The origins, branches, distribution and anastomosis of nutrient vessels of the inferior 1/3 segment of lateral antebrachial cutaneous nerve-cephalic vein; ② The relationship of nutrient vessels of lateral antebrachial cutaneous nerve-cephalic vein and radius periosteal vessels.RESULTS: ① The origins of nutrient vessels supplying the inferior 1/3 segment of the lateral antebrachial cutaneous nerve were: 6 to 11 cutaneous branches of radial artery with an outer diameter of (0.7±0.3) mm, 2 to 6 cutaneous branches of superficial palmar branch, with an outer diameter of (0.5±0.3) mm, 1 to 2 cutaneous branches of recurrent branch nourishing the radial styloid process with an outer diameter of (0.8±0.1) mm, 1 to 2 cutaneous branches nourishing tabatiere anatomique with an outer diameter of (0.6±0.2) mm. 8.0 to 15.0 cm superior to the radial styloid process, there were 1 to 3 spatium intermuscular osteocutaneous perforators of radial artery with an outer diameter of (1.1 ±0.2) mm, and periosteal branches distributed in the denudate area of the middle-inferior radial bone with its cutaneous branches (0.8±0.2) mm in diameter. Above perforators gave rise to cutaneous branches, fascia branches,periosteal branches and nutrient vessels of nerve to superficial vein to form a vessel chain of cutaneous nerve-cephalic vein and vascular networks of deep fascia, superficial fascia and periosteum.CONCLUSION: The nutrient vessels of lateral antebrachial cutaneous nerve-cephalic vein and the nutrient vessels of muscle, bone and skin are homologous. The compound flap of lateral antebrachial cutaneous nerve-cephalic vein pedicled with the cutaneous branches of radial artery can be applied to repair the distal tissue defects of the hand by rotating the flap on the plane of wrist joint.

Objective To explore the effect of thoracic paravertebral block (TPVB)versus general anaesthesia (GA)on breast surgery.Methods Public databases were searched including Med-line and Springer,in order to collect randomized controlled trial (RCT)of the effects of TPVB and GA on breast surgery.The data of the meta-analysis was analyzed by Stata 11.0 and RevMan 5.1. Results There were five RCTs consisting of 295 patients with breast surgery,group TPVB (n=148) and group GA (n=147).The overall results of meta-analysis showed that pain scores of group TPVB were higher than those of group GA for breast surgery patients (SMD 2.59,95%CI 1.10-4.08),but the postoperative analgesic consumption and postoperative nausea or vomiting of group TPVB were lower than those of group G (RR 0.23,95%CI 0.15-0.37 and RR 0.27,95%CI 0.12-0.61,respec-tively).Conclusion Compared with GA,TPVB has higher pain scores,lower postoperative anal-gesic consumption and postoperative nausea or vomiting.

WAE. Conclusion SBE method is better than the other three methods in the extraction of SFD components.

Objective To assess the effects of three way catalytic converter on the health risk of benzene series.Methods After collecting the benzene series(benzene,toluene,xylene) in the air inlet and air outlet of the three way catalytic converser in 100% working loads,their health risk was assessed with the methods of health risk assessment.Results After catalysis,the hazard index of toluene and xylene decreased in all loads,the hazard index and carcinogenesis risk of benzene increased in 100% loads,the chronic long-term hazard index of benzene deceased in 100% loads.Conclusion The three way catalytic converter can change the health risk of benzene series emission by gasoline engine,the carcinogenesis risk of benzene series emission will be increased when the gasoline is treated with the three way catalytic converter.

60,≤60 year age groups and control group was 2.49?1.82,1.82?1.58,0.90?1.38, significantly decreased(P

AIM: To study the physiological function changes of the tears film after rebuilding ocular surface with corneal stem cells, and to discuss the validity and the estimate system of rebuilding ocular surface with the corneal stem cells. METHODS: The male New Zealand rabbits were used to establish the alkali burning model in the right eye. The corneal stem cells of the left eye were cultured on the amniotic membrane in vivo, and then transplanted to the right eye. Furthermore, the physiological function changes of the tear film were examined. RESULTS: Compared to the before alkali burning, the ocular surface cell morphology was similar after rebuilding ocular surface with the corneal stem cells, which were cultured on the amniotic membrane in vivo; The tear film breakup time test showed the a remarkable difference between after and before the alkali burning, but the cell modality after rebuilding had no remarkable difference compared to that before the alkali burning. CONCLUSIONS: It's an effective method to rebuild the ocular surface with the corneal stem cells in vivo, the cell framework and the physiological function of the tears film recover well after rebuilding. It may be a validity estimate system of rebuilding ocular surface to analyze framework and configuration of the ocular surface and test the tear film breakup time.

T lymphoblastic leukemia/lymphoma (T-ALL/LBL) is a kind of lymphoblastic malignant tumors orientated in T-cell line with poor clinical effects. Its molecular genetics is mainly correlated with antigen receptor gene, abnormal chromosome, oncogene activation, tumor-suppressor gene inactivation and molecular pathways. This article will review the latest research progress of the molecular genetics in T-ALL/LBL to explore the index of prognosis and possible therapeutic targets.